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Ovitrap use in epidemiology study of Aedes aegypti and Aedes albopictus in Kuta Alam sub-district Banda Aceh, Indonesia Athaillah Farida; Abu Hasan Ahmad; Upik K. Hadi; Muhammad Hambal; Yudha Fahrimal; Rimaya Shafitri
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

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Abstract

A study on the mosquitoes Aedes spp. has been conducted in Kuta Alam Sub-district, Banda Aceh, to find out the percentage and distribution of positive ovitrap with eggs of Aedes aegypti and A. albopictus, based on location of ovitrap, level of rainfall as well as different landuse of settlement areas. Sampling was conducted by setting up ovitrap monthly, from January to December 2010 in settlement area that have more trees versus less trees, both indoor and outdoor. Data of the ovitrap percentage that was proved to be positive with eggs of A. aegypti and A. albopictus was analized using Student T-Test. The study revealed that rainfall value did not significantly affect (P0.05) the percentage of positive A. aegypti and  A. albopictus ovitraps and neither  did the location of ovitrap (indoor versus outdoor). The settlement areas with more trees have significantly higher percentage of ovitraps containing A. albopictus than areas with less trees, but not significant for A. aegypti (P0.05). It was suggested that rainfall and location of ovitrap did not influence the number of ovitrap containing Aedes aegypti and Aedes albopictus eggs, while settlement landuse  influenced significantly the percentage of positive ovitraps for Aedes albopictus, but not for Aedes. aegypti. Between two villages in Kuta Alam sub-district, it was found out that Gampong Beurawe was dominated by Aedes albopictus while Gampong Kuta Alam by Aedes aegypti. Overall, the sensitivity of ovitrap in Kuta Alam subdistrict was 83,7%. The usage of ovitrap is thus recommended for monitoring of Aedes spp. control programs
Histopathological changes in intestine of chicken (Gallus domesticus) infected naturally by Ascaridia galli Ummu Balqis; Muhammad Hambal; . Darmawi; Cut S. Utami
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Abstract

This study was aimed at finding out the histopathological changes of chicken intestines that were naturally infected by Ascaridia galli. Ten intestines were obtained from wet market in Banda Aceh then measured in length and divided into three sections: duodenum, jejunum and ileum, of which histologicslides were made. The number of Ascaridia galli founded in the lumen and ingesta were counted. Parameter picture of histopathologycal changes were analized descriptively based on desquamation, hiperplasia, and villi fussion occure. Duodenum, jejunum and ileum infected by A. galli showed istopathological changes of villi the cover of desquamation, hyperplasia and fusion. Demage level of the small intestine chicken was largely determined by the number of infecting by A. galli. It was concluded that the more number of A galli infected to chickens, the higher level of damage would be occured in the small intestine of chicken
Enzyme activity of Ascaridia galli Llarvae was inhibited by Phenil Methanyl Methane Sulfonyl Fluoride Ummu Balqis; . Darmawi; Muhammad Hambal
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

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Abstract

Enzyme catalyse a broad spectrum of important biological reactions, including protein metabolism and immune reactions. A study was carried out to partial characterize enzyme from exretory/secretory of A. galli L3 stage based on influence of inhibitor. A. galli L3 were recovered from intestines of 100 heads chickens 7 days after oesophagus inoculation with 6000 L2. L3 recovered in this manner were cultured (5 – 10 ml-1) in flasks containing rosswell park memorial institute (RPMI) 1640 media, pH 6.8, without phenol red. Cultures were incubated at 370C in 5% CO2 and culture fluid was collected after 3 days in culture. Excretory/secretory was prepared from metabolic product of L3 released in culture medium. The enzyme activity was assayed against casein 2%. The amount of concentration was determined from the absorbance at 595 nm. Inhibitor sensitivity on enzyme activity was studied in phenil methanyl methane sulfonyl fluoride (PMSF) 0,5 and 1 mM.    The result showed that enzyme activity of A. galli larvae was inhibited by PMSF 0.5 and 1 mM, which enzyme activity remainded 1,8 and 0%, respectively. The results indicate that the excretory/secretory secreted by L3  A. galli contained serine protease
Mucosal Mast Cells Contribution in Intestinal Defense of Chickens (Gallus domesticus) Infected Naturally by Ascaridia galli Ummu Balqis; Masda Admi; Darmawi Darmawi; Muhammad Hambal
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

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Abstract

This study was aimed at finding out the investigation of mucosal mast cells   in intestines of chicken  that  were  naturally  infected  by  Ascaridia galli.  Amount  of  ten  intestine  of  freshly slaughtered chickens (Gallus domesticus) found from local abatoir in Banda Aceh were divided into two groups containing five intestines of each. Mucosal mast cells count were done of which histologic slides were made in stained serial histological sections with Alcian blue (pH 0,3) and Safranin-O (pH 0,1) of the intestines. The result showed that the mucosal mast cells increased significantly (P 0.05) in intestines of chickens infected naturally by survival A. galli adult worms. It was concluded that the intestinal defense of chickens against parasite infection is associated with the mucosal mast cells contribution by creating an environment hostile to the establishment and survival of intestinal nematodes, A. galli.
Poultry sellers’ perception and their effort for prevention strategy on avian influenza in Banda Aceh, Indonesia . Darmawi; Teuku R. Ferasyi; . Muthmainnah; Ummu Balqis; . Fakhrurrazi; . Darniati; Muhammad Hambal
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Abstract

A study was conducted to examine some poultry sellers’ perception of the Avian influenza (birdflu) in nine locations (Ulee Kareng, Batoh, Lamnyong, Kampong Baru, Peunayong, Setui, Kampong Ateuk, and Neusu) in Banda Aceh Municipality, Indonesia. Primary data were obtained with the use of a structured questionnaire administared to ninety nine (99) respondents selected using proportional stratified random sampling, between November to December 2011. Information was obtained using the questionnaire sheets contain questions: knowladge, attitude, availability of facilities, and the effort of Avian influenza prevention strategy. Data obtained were analyzed by descriptive statistics. The result showed that only 52% of the poultry sellers’ had good knowledge of early symptomps of Avian influenza. Amount 59% of the poultry sellers’ had good attitudes. Result also showed that 73% of respondents had good facilities that was available in live poultry market area, but only 70% of respondents were good awareness about the effort of Avian influenza prevention strategy. In addition, the respondents who had more knowledge, good attitudes, and/or good facilities were also those who actually acted more preventively. Based on the result we concluded that the poultry sellers’ perception on prevention strategy for infection of birdflu in Banda Aceh categorized as poor. It is suggested that compliance with preventive measures may be enhanced through behavioural modification and building awareness through communication, training, workshop programs to the live poultry market sellers’ in Banda Aceh
Inhibition of Salmonella sp. Growth in Comparison with Antibiotics and Pineapple (Ananas comosus) Squeeze Masda Admi; Erina Erina; Azhari Azhari; Ummu Balqis; Muhammad Hambal; Darmawi Darmawi
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
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Abstract

The purpouse of this study to examine antibiotics inhibition growth of Salmonella sp. isolated from feces of broiler chickens to chloramphenicol, gentamicin, and tetracycline in coparison with pineapple squeeze. Swab  sample  from  broiler  chicken  cloaca  was  cultured  to  nutrient  broth  media  and incubated at 37°C temperature for 24 hours. Culture was compared with Mc Farland 3 standardization, and separated on surface of Muller Hinton Agar (MHA). Antibiotic sensitivity was tested by mean of  chloramphenicol, gentamisin, and tetrasiklin in coparison with pineapple squeeze. The antibiotic disks (Oxoid) were put on the surface of MHA media which had inoculated with Salmonella sp. bacteria. Antibiotic sensitivity was determined based on formation of inhibition zone in surrounding of antibiotic disc. The result showed that Salmonella sp. bacteria growth was inhibited in ≥ 21 mm and ≥ 18 mm, ≥ 7 mm and ≥ 9 mm against chloramphenicol, gentamisin, tetrasiklin, and pineapple squeeze respectively. This research concluded that the Salmonella sp. cultured in this manner were sensitive to chloramphenicol and gentamisin. The inhibition growth of Salmonella sp. caused by chloramphenicol and gentamicin was significantly higher in comparison with pineapple (Ananas comosus) squeeze, meanwhile the Salmonella sp. bacteria was resistance against tetracycline. Our result suggest that both antibiotics chloramphenicol and gentamicin were recommended for antibiotic therapy in Salmonella sp. infection.
IDENTIFIKASI ENDOPARASIT PADA IKAN JEBONG (Abalistes stellaris) DI TEMPAT PELELANGAN IKAN (TPI) LAMPULO KOTA BANDA ACEH Muhammad Geraldy Zarry; Muhammad Hambal; Zuhrawaty N.A.
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 2 (2017): FEBRUARI - APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.774 KB) | DOI: 10.21157/jim vet..v1i2.2729

Abstract

ABSTRAK Penelitian ini bertujuan untuk mengidentifikasi endoparasit pada Starry Triggerfish (Abalistes stellaris) yang diperoleh dari Tempat Pelelangan Ikan (TPI) Lampulo Banda Aceh. Penelitian dilakukan pada Mei 2016 di Laboratorium Parasitologi, Fakultas Kedokteran Hewan Unsyiah. Sebanyak 25 Abalistes stellaris diperoleh dan kemudian diperiksa untuk mengetahui keberadaan endoparasit. Sebelum diperiksa, panjang ikan diukur dan berdasarkan pada keberadaan telur di perut, jenis kelamin ikan diidentifikasi. Pengamatan dari endoparasit dilakukan dengan mengamati organ internal ikan secara makroskopik kemudian mikroskopik. Hasil penelitian menunjukkan bahwa ikan terinfeksi oleh Anisakis spp. dan Tetraphylidea di perut dan usus ikan dengan tingkat prevalensi masing-masing endoparasit antara 12% dan 36%. Hasil juga menunjukkan bahwa semakin panjang tubuh ikan, semakin banyak parasit yang diperoleh.Kata Kunci: Abalistes stellaris, Anisakis spp., endoparasit ABSTRACT      This study aims to identify endoparasite on Starry Triggerfish (Abalistes stellaris) obtained from the Lampulo Fish Auction Site (TPI) Banda Aceh. The study was conducted in May 2016 at the Laboratory of Parasitology, Faculty of Veterinary Medicine Unsyiah. As many as 25 Abalistes stellaris were collected and then examined to find out endoparasites. Before examined, the length of fish were measured and based on the existence of spawn in the abdomen, the sex was identified. Observations of the endoparasites were done by observing internal organs of fish macroscopic then microscopic. The results revealed that the fish were infected by Anisakis spp. and Tetraphylidea in the stomach and intestines of fish with a prevalence rate of each endoparasite between 12% and 36%. Results also showed that the longer the fish length is, the more parasites were obtained.Keywords: Abalistes stellaris, Anisakis spp., endoparasites
GAMBARAN HISTOPATOLOGI OTOT FEMUR KELINCI (LEPUS SP.) SETELAH IMPLAN PLAT LOGAM (Histopathology Of Rabbit’s (Lepus sp.)Femur Muscle After Metal Plate Implantation) Muhammad Fathur Ridho; Erwin Erwin; Ummu Balqis; Muhammad Hambal; Syafruddin Syafruddin; Mulyadi Adam
JURNAL ILMIAH MAHASISWA VETERINER Vol 5, No 3 (2021): MEI-JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/jim vet..v5i3.12878

Abstract

ABSTRAKPenelitian ini bertujuan untuk mengetahui perbedaan gambaran histopatologi otot femur kelinci (Lepus sp.) setelah implan plat besi dan tantalum. Kelompok perlakuan 1 (K1) kelinci yang diimplantasi menggunakan plat tantalum dan kelompok perlakuan 2 (K2) kelinci yang diimplantasi menggunakan plat besi di antara Musculus Vastus Lateralis dan tulang femur selama 2 bulan. Perubahan histologi yang diamati adalah ada tidaknya nekrosis, fibrosis dan tissue cavities pada jaringan otot femur kelinci. Hasil pengamatan secara mikroskpois  ditemukan fibrosis, tetapi tidak ditemukan nekrosis dan tissue cavities. Terdapat perbedaan gambaran histopatologi otot kelinci (Lepus sp.) setelah penggunaan implan plat tantalum dan plat besi. Kata kunci : Kelinci, Tantalum, Besi, Histopatologi, Otot, Fibrosis, Nekrosis, Tissue Cavities. ABSTRACT                    The aim of this study was to determine the differences in the histopathology of the rabbit femur (Lepus sp.) Muscle after iron plate and tantalum implants. The first treatment group (K1) rabbits implanted using tantalum plates and treatment group 2 (K2) rabbits were implanted using an iron plate between the Musculus Vastus Lateralis and the femur bone for 2 months. The histological changes observed were the presence or absence of necrosis, fibrosis and tissue cavities in the muscle tissue of the rabbit femur. The results of the study showed that fibrosis was found, but necrosis and tissue cavities were not found. There are differences in the histopatological muscle of rabbit (Lepus sp.) after the use of tantalum plate and iron plate implants. Keywords: Rabbit, Tantalum, Iron, Histopathology, Muscle, Fibrosis, Nekrotic, Tissue Cavities.
PEMERIKSAAN KEBERADAAN TELUR DAN LARVA NEMATODA PASCA PEMBERIAN ANTHELMINTIK PADA GAJAH SUMATERA (Elephas maximus sumatranus) DI CONSERVATION RESPONSE UNIT (CRU) SAMPOINIET ACEH JAYA Ova and Nematode larvae examination after anthelmintics treatment on Sumatra Elephant (Elephas maximus sumatranus) in Conservation Response Unit (CRU) Aceh Jaya Sampoiniet syafriza harliyanda; Muhammad Hambal; Arman Sayuti
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 3 (2017): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (487.15 KB) | DOI: 10.21157/jim vet..v1i3.3461

Abstract

ABSTRAKPenelitian ini dilakukan untuk mengetahui keberadaan telur dan larva nematoda pasca pemberian anthelmintik pada gajah sumatera (Elephas maximus sumatranus) di Conservation Response Unit (CRU) Sampoiniet Aceh Jaya dan Pusat Konservasi Gajah (PKG) Saree. Sampel feses gajah sumatera diambil dari 4 ekor gajah yang ada di CRU Sampoiniet Aceh Jaya dan 1 ekor gajah yang ada PKG Saree. Pengambilan sampel feses gajah Sumatera dilaksanakan sebanyak 3 kali seminggu. Hasil penelitian menunjukkan tidak ditemukan telur maupun larva pada gajah-gajah yang berada di CRU Sampoiniet. Ditemukan telur cacing Ascaris sp dan Toxocara spp serta ditemukan juga larva dari cacing Rhabiditiform dan Ascaris sp pada gajah di PKG Saree. Efikasi obat cacing masih optimum selama 3 minggu pasca pemberian obat cacing.Kata kunci: Telur cacing nematoda Ascaris sp, Toxocara spp, Larva Rhabiditiform, Ascaris sp, Gajah sumatera (Elephas maximus sumatranus)ABSTRACT This research was conducted to determine the eggs and larvae of namatode after anthelmintic administation on sumatran elephant (Elephas maximus sumatranus) in Conservation Response Unit (CRU) Sampoinet Aceh Jaya and Conservation Center of Elephant (CCE) Saree. The  Sample feces of sumatran elephant takes from 4 elephant in CRU Sampoiniet Aceh Jaya and 1 elephant in CCE Saree. The results showed no eggs or larvae are found on the elephants residing in CRU Sampoiniet. Ascaris worm eggs found sp and Toxocara spp and found larvae of Ascaris sp and Rhabiditiform worms in CCE Saree. The feces sample of sumatran elephant was taken three times, conducted every week. It was conducted, the elephant in Sampoiniet could not be found the eggs of parasite in feces due to anthelmintic treatment.Keywords: Egg worm nematodes Ascaris sp, Toxocara spp, Rhabiditiform larvae, Ascaris sp, Sumatran elephant (Elephas maximus sumatranus)
PENGARUH EKSTRAK KUNYIT (Curcuma domestica) KONSENTRASI 75 % TERHADAP MOTILITAS DAN MORTILITAS CACING Ascaridia galli SECARA IN VITRO (Effect of turmeric extract (Curcuma domestica) 75% concentration against motility and mortality of Ascaridia galli in vitro) zena fisdiora; ummu balqis; muhammad hambal
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 2 (2018): FEBRUARI - APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.948 KB) | DOI: 10.21157/jim vet..v2i2.6860

Abstract

Penelitian ini bertujuan memberikan informasi tentang pengaruh ekstrak kunyit (Curcuma domestica) dengan konsentrasi 75% terhadap motilitas dan mortalitas cacing Ascaridia galli secara In vitro. Sampel yang digunakan adalah cacing Ascaridia galli betina dewasa yang aktif bergerak, sehat serta memiliki panjang 7-11 cm sebanyak 6 ekor. Ascaridia galli dibagi menjadi 2 kelompok perlakuan, masing-masing perlakuan terdiri atas 3 ekor. Untuk kelompok I Ascaridia galli direndam dalam larutan NaCl 0,9% sebagai kontrol , sedangkan untuk kelompok II Ascaridia galli direndam dalam ekstrak kunyit (Curcuma domestica) konsentrasi 75%. Parameter penelitian di ukur berdasakan nilai skor dengan kriteria, untuk nilai skor 3 diberikan apabila seluruh tubuh cacing bergerak, nilai skor 2 diberikan apabila hanya sebagian tubuh cacing yang bergerak, nilai skor 1 diberikan apabila cacing tidak bergerak (diam) tetapi masih hidup, skor 0 diberikan apabila cacing tidak bergerak (mati). Hasil penelitian menunjukkan bahwa cacing Ascaridia galli yang direndam dalam ekstrak kunyit (Curcuma domestica) konsentrasi 75% menunjukkan kematian pada jam ke-3, sedangkan cacing Ascaridia galli yang direndam dalam larutan NaCl 0,9% mati pada jam ke-8. Ekstrak kunyit (Curcuma domestica) dengan konsentrasi 75% menyebabkan kematian cacing Ascaridia galli 5 jam lebih cepat dibandingkan dengan NaCl 0,9% secara In vitro. Hasil Penelitian ini dapat disimpulkan bahwa ekstrak kunyit (Curcuma domestica) konsentrasi 75% memiliki potensi sebagai anthelmentik untuk cacing Ascaridia galli. (This study provides information on the use of turmeric extract (Curcuma domestica) with a concentration of 75% agains Ascaridia galli in vitro. Sample that was used were 6 female adult of Ascaridia galli that are still active, healthy and have a length of 7-11 cm, Ascaridia galli was divided into 2 groups of treatment, each consists of 3 Ascaridia galli. In group I Ascaridia galli was rinsed in 0.9% NaCl solution as control, while in group II Ascaridia galli was rinsed in turmeric extract (Curcuma domestica) with concentration of 75%. The parameters in this study were measured based on criteria, for score 3 given the chance of the whole body of the worm moves, the score of 2 was given while only part of the body moving,  score 1 was given when the worms were not moving but still shows living signs, the score 0 was given when worms were not moving at all (dead). The results showed that Ascaridia galli rinsed in turmeric extract (Curcuma domestica) with concentration 75% died with in 3 hours, while Ascaridia galli rinsed in 0.9% NaCl solution having the time of death after 8 hours. This research concluded that turmeric extract (Curcuma domestica) concentration of 75% had effect on motility and mortality of Ascaridia galli in vitro and has anthelmintic potentiates for Ascaridia galli)
Co-Authors . Darmawi . Darmawi . Darniati . Fakhrurrazi . Muthmainnah Abd. Rasyid Syamsuri Abdul Harris Abu Hasan Ahmad Abu Hassan Ahmad Agus Dermawan Amalia Sutriana Amiruddin A Anugrah Septian Arman Sayuti Athaillah Farida Atika Agusty Audi Maldini Aulanni'am, Aulanni'am Azhari Azhari Basuki B. Purnomo Budianto Panjaitan Cut Dahlia Iskandar Cut S. Utami Darmawi Darmawi Dewi Mahartina Dian Masyitha Dwina Aliza Dwinna Aliza Elia Wardani Ellyawardani Ellyawardani Elsa Mariane Ramadani Erdiansyah Rahmi Erina Erina Erwin Erwin Fadli A. Gani Fakhrurrazi Fakhrurrazi Farid Wajidi Farida Athailah Farida Athaillah Farida Farida Gholib Gholib Hamdani Budiman Hamny Sofyan Henni Vanda Ikwan Jamil Ismail Ismail Joharsyah J M Daud AK M Hasan M. Aris Widodo M. Nur Salim Maria Ferawati Magai Masda Admi Muhammad Fathur Ridho Muhammad Geraldy Zarry Muhammad Hanafiah Muhammad Ridhan Akbar Mulya Fahmi Mulyadi Adam Murhaban Murhaban Muslim Akmal Mustafa Sabri Muttaqien Bakri Muttaqien Bakrie Muttaqien Muttaqien Nellita Mutia NURLIANA NURLIANA Nuzul Asmilia Poedji Hastutiek Rastina Rastina Razali Daud Razali Razali Resty Fauzana Rahman Rimaya Shafitri Rinidar R Rizka Ayuni Roslizawaty R Siti Aisyah Soeharsono Soeharsono Sri Mulyati Sri Wahyuni Sugito Sugito Suriadi S Sutiman B. Sumitro syafriza harliyanda Syafruddin S Syafruddin Syafruddin t fadrial karmil T Reza Ferasyi T. Armansyah T. Reza Ferasyi Tapielaniari - tati maulidya Taufikah Rahmah Teuku R. Ferasyi Teuku Reza Ferasyi Tongku Nizwan Siregar Triva Murtina Lubis Ummu Balqis Upik K. Hadi Utari Desya Winaruddin Winaruddin Wulan Windasari Yudha Fahrimal Zainuddin Z Zainuddin Zainuddin zena fisdiora Zuhrawaty N.A. Zuhrawaty NA