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All Journal The Journal of Experimental Life Sciences (JELS) Biotropika Advance Sustainable Science, Engineering and Technology (ASSET)
Agung Pramana Warih Marhendra
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Neuroscience

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The Effect of Alpinia Purpurata K. Schum Rhizome Extract Supplementation in Tris-Egg Yolk Extender on the Quality of Cryopreserved Bali Bull Sperm Firlina Laila Putri; Sri Rahayu; Agung Pramana Warih Marhendra
The Journal of Experimental Life Science Vol. 13 No. 2 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.02.03

Abstract

This study aimed to analyze the effect of Alpinia purpurata K. Schum rhizome extract (APRE) supplementation into tris-egg yolk extender on the sperm quality of Bali bull after freezing. This study used experimental method with a completely randomized design (CRD), consisting of five treatments, denoted as P0 (semen diluted using 100% Tris-Egg Yolk (TEY)), P1 (semen diluted using 98% TEY and 2% APRE), P2 (semen diluted using 96% TEY extender + 4% APRE), P3 (semen diluted using 94% TEY + 6% APRE), and P4 (semen diluted using 92% TEY + 8% APRE). Semen quality was assessed both before and after freezing, using several indicators such as motility, viability, and abnormality of sperm. The antioxidant activity of RGE was determined using the DPPH (1,1-diphenils-2-picrylhydrazils) technique. The data were analyzed using ANOVA with further analysis using post hoc tests in the SPSS 25 program. The results showed that APRE has strong antioxidant activity, with an IC50 value 38.004 μg.mL-1. Meanwhile, this research has shown that the addition of 6% RGE in the tris-Tris-egg yolk extender was able to maintain sperm quality before and after freezing. Keywords: Alpinia purpurata K. Schum, sperm quality, tris-egg yolk.
Effect of Combination between the Extract of Marsilea crenata and Alpinia purpurata K.Schum Rhizome on Sperm Quality of Hyperglycemia Mice Shofi Nur Aliyah; Sri Rahayu; Agung Pramana Warih Marhendra
The Journal of Experimental Life Science Vol. 13 No. 3 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.03.06

Abstract

This study aims to analyze the effect of the combination between water clover (Marsilea crenata) and red galangal rhizome (Alpinia purpurata) on sperm quality of hyperglycemia model mice. Mus musculus were randomly divided into eight groups: non-hyperglycemia (P0), hyperglycemia (P1), metformin 0.375 mg.kg-1 (P2), hyperglycemia with M. crenata extract dose 0.09 mg.g-1 (P3), A. purpurata dose 0.20 mg.g-1 (P4), combination of M. crenata (0.09 mg.g-1) - A. purpurata (0.20 mg.g-1) (P5), combination of M. crenata (0.09 mg.g-1) - A. purpurata (0.40 mg.g-1) (P6), and combination of M. crenata (0.18 mg.g-1) - A. purpurata (0.20 mg.g-1) (P7). Hyperglycemia mice were induced by streptozotocin 145 mg.kg-1. STZ induction and oral extract addition were carried out for 20 days. Sperm quality parameters observed in this study included motility, viability, and concentration. Sperm quality data were statistically analyzed with One-Way ANOVA followed by the Tukey HSD test using the SPSS 23 for Windows program (p<0.05). This research demonstrates that adding M. crenata, A. purpurata, and their combination for 20 days can significantly increase motility, viability, and sperm concentration in hyperglycemia-model mice. The combination of M. crenata and A. purpurata in the ratio of 1:1 was considered effective in improving the sperm quality of hyperglycemia model mice. Keywords: A. purpurata, hyperglycemia, M. crenata, oxidative stress, sperm quality.
Predicting Habitat Suitability of Mahseer Fish (Tor spp.) in Tropical River Systems Using MaxEnt and Google Earth Engine: A Geospatial Modeling Approach Jefri Permadi; Nia Kurniawan; Diana Arfiati; Agung Pramana Warih Marhendra
Advance Sustainable Science Engineering and Technology Vol. 7 No. 3 (2025): May - July
Publisher : Science and Technology Research Centre Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/asset.v7i3.2201

Abstract

Rivers are vital freshwater habitats that face threats of degradation and climate change. Mahseer fish, a key species, is in decline. This study predicted Mahseer fish habitats in Central Java using the Google Earth Engine and the MaxEnt machine learning algorithm. Environmental predictors, including NDVI, elevation, slope, river order, temperature, and rainfall, were extracted from Sentinel, SRTM, MODIS, and CHIRPS data. The model identified river order as the most influential variable (73%), followed by elevation (18%) and rainfall (8%), with an AUC score of 0.7, indicating fair accuracy. Suitable habitats were located in upstream river orders (1–3), typically at higher elevations. These findings provide spatial guidance for conservation planning, such as identifying critical habitats, prioritizing upstream areas, and establishing seasonal fishing ban. This approach supports biodiversity protection and aligns with the Sustainable Development Goals by offering a scalable tool for freshwater ecosystem management. Using MaxEnt with GEE shows promise for rapid, and cost-effective species distribution modeling in data-limited tropical regions.
Effect of Aqueous Basil Extract (Ocimum sanctum) on Monosodium Glutamate-Induced Mice Sperm Aris Soewondo; Sri Rahayu; Agung Pramana Warih Marhendra; Nurul Shafa Paramita; Anaqoh Roudhotul Jannah Winarso
Biotropika: Journal of Tropical Biology Vol. 13 No. 2 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.02.01

Abstract

This study aims to analyse the protective effect of aqueous extract of basil leaves (Ocimum sanctum) on the sperm quality of monosodium glutamate (MSG)-induced mice. This study used 30 male mice (aged 4-5 weeks old and weighing 25-35 grams), which were then randomly divided into five groups: P0 (healthy male mice), P1 (male mice induced with 4mg/kg BW MSG), P2, P3, P4 (male mice induced with MSG and given aqueous extract of basil leaves (O. sanctum) 300mg/kgBB, 500mg/kgBB, 700mg/kgBB for 14 days). At the end of treatment, all mice will be sacrificed and cauda epididymis was isolated. The obtained semen was analysed for sperm quality, including motility, viability, concentration, and spermatozoa abnormalities. Data analysis used one-way ANOVA and continued with the Duncan test using SPSS for Windows. In this study, the administration of 700mg/kg BW aqueous extract of basil leaves (O. sanctum) could improve sperm quality by increasing motility, viability, and concentration of sperm and also alter the sperm abnormality of MSG-induced mice.
Co-Authors Anaqoh Roudhotul Jannah Winarso Aris Soewondo Diana Arfiati Firlina Laila Putri Jefri Permadi Nia Kurniawan Nurul Shafa Paramita Shofi Nur Aliyah SRI RAHAYU