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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Jurnal Keperawatan Soedirman (The Soedirman Journal of Nursing) Indonesian Journal of Biotechnology INDONESIAN JOURNAL OF PHARMACY Sari Pediatri Paediatrica Indonesiana Belitung Nursing Journal Jurnal Kesehatan Terpadu (Integrated Health Journal) Journal of Community Empowerment for Health JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Berita Kedokteran Masyarakat
Hera Nirwati
Departemen Mikrobiologi, Fakultas Kedokteran, Kesehatan Masyarakat, Dan Keperawatan, Universitas Gadjah Mada
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Journal : INDONESIAN JOURNAL OF PHARMACY

 1 
IDENTIFICATION OF THE VIRUS DENGUE–3 EPITOPE’S IMMUNODOMINAN USING THE SYNTHETIC PEPTIDE Nirwati, Hera; ., Sutaryo; Wahyono, Djoko
Indonesian Journal of Pharmacy Vol 13 No 1, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.512 KB) | DOI: 10.14499/indonesianjpharm0iss0pp26-33

Abstract

Dengue virus infection has been known as an important health problem in many tropical countries, because of increasing number of patients, expansion of epidemic areas and emergence of severe clinical manifestations. Dengue virus consists of 3 structural and 7 nonstructural proteins. The major virion surface protein, the envelope protein, is the most important antigen with regards to virus biology and humoral immunity. Synthetic peptide derived from the envelope protein gene sequence can be used to identify region of the envelope protein that elicits antibodies. One hundred and sixty-one synthetic peptides were synthesized based on sequence of protein envelope dengue-3 virus published by Osatomi and Sumiyoshi. Each peptide consists of 15 amino acids with the last 12 amino acids overlapped. Peptide synthesizer, auto-spot robot ASP 222 (Abimed), was used for synthesis using solid spot method developed by Ronald Frank. Synthetic peptides attached to nitrocellulose membrane were used for spot immunoassay of 6 normal human sera, 6 dengue infected human sera and  6 non dengue-infected. Nine peptides were specific for dengue infected sera and these might be candidate immunodominat epitopes of dengue virus-3. They were EGLSGATWVDVVLEH (amino acids number 13-27), VCKHTYVDRGWGNGC (91-105),SIEGKVVQHENLKYT (124-138), TVHTGDQHQVGNETQ (142-156), TLGLECSPPTGLDFN (178-192), KGEDAPCKIPFSTED (325-339), PFSTEDGQGKAHNGR (334-348), GARRMAILGDTAWDF (406-420) and KIGIGVLLTWIGLNS (454-468). Based on spot immunoassay, 11 peptides were synthesized and used them for ELISA of 15 normal sera, 22 non-dengue infected sera and 37 dengue sera. Peptides BTLDDIELQKTEATQLA, BPFSTEDGOGKAHNGR and BKKEEPV NIEAEPPFG showed significantly different in their reactivities to the three groups. Combinations of two peptides were used for ELISA. Only one of them (BKGEDAPOKIPFSPED and BRMAILGD TAWDFGSV) showed significantly different in their reactivi- ties to the three groupsKey words: synthetic peptide, envelope protein dengue-3, spot immunoassay
Quantitative Analysis of Multi-components in Curcuma xanthorrhiza by Single Marker Badrunanto Badrunanto; Wulan Tri Wahyuni; Hera Nirwati; Mohamad Rafi
Indonesian Journal of Pharmacy Vol 30 No 4, 2019
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (936.617 KB) | DOI: 10.14499/indonesianjpharm30iss4pp301

Abstract

A new simple and effective routine analytical method for quantification of curcuminoids in Curcuma xanthorrhiza was developed by high-performance liquid chromatography. This method based on chromatographic fingerprint combined with a quantitative analysis of multi-components by single marker (QAMS). Curcumin was selected as an internal marker for the determination of two other similar compounds, i.e. bisdemethoxycurcumin and demethoxycurcumin, by using the relative coefficient factor (RCF). Excellent linearity was obtained for each component (r2 > 0.9998), and the recovery of extraction methods were within 100.23-103.95%. The precision of the method was good at inter-day and intra-day analysis (RSD < 4.0%). The stability of RCFs was good under various chromatographic conditions with RSD < 1%, and the ratio of retention time was used to locate each compound. The quantification of curcuminoids between QAMS and external standard method (ESM) proved the consistency and similarity of the two method (RSD < 2%). This study demonstrated that QAMS could be used as a routine method for quality control of curcuminoids in C. xanthorrhiza. This method successfully proved accurate, stable, more effective and simple than external standard method.
Co-Authors Abu Tholib Aman Achirul Bakrie Achmad, Bayu Fandi Aris Haryanto Aris Haryanto Badrunanto Badrunanto Chandra, Lukman Ade Dibtyanusa, Ajib Dwi Aris Agung Nugrahaningsih Dwiprahasto, Iwan Ema Damayanti Eti Nurwening Sholikhah Fatwa Sari Tetra Dewi Handi Virawan Haryani - Ibrahim Rahmat Ika Puspitasari JAKA WIDADA Khudazi Aulawi Kian Sinanjung Kumara, Intan Fatah Lutfan Lazuardi Lutfan Lazuardi M. Rafi Mahatma Sotya Bawono Meri Afridayani Mustofa Mustofa Nastiti Wijayanti Nastiti Wijayanti Nenny S Mulyani Raden Sumiharto Ramadhanti, Ahijrah Rinaldi Daswito Ririn Pakaya Rukmono Siswishanto S. Sutaryo Santri, Ichtiarini Nurullita Setyawan Bekti Wibowo Siswanto Siswanto Sutaryo Sutaryo Sutaryo1 S, Sutaryo1 Sutono Sutono Titien Budhiaty Titik Nuryastuti Titis Widowati Titis Widowati Tri Wibawa Wahyono, Djoko Wulan Tri Wahyuni Yati Soenarto Yohana Ika Prastiwi