Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Nexus Biomedika Nexus Kedokteran Translasional
Endang Listyaningsih Suparyanti
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
The Effect of Strawberry (Fragaria x ananassa) Fruit Juice on Renal Cell Histological Damage of Mice Induced by Rhodamine B Dea Saufika Najmi; Endang Listyaningsih Suparyanti; Yulia Sari
Nexus Biomedika Vol 4, No 1 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.309 KB)

Abstract

Background:Strawberry (Fragaria x ananassa) contains many antioxidant phytochemicals such as vitamin C, anthocyanins and ellagic acid that may exhibit significant protection of kidney cells from free radicals. In present study, Strawberry (Fragaria x ananassa) fruit juice was evaluated for its nephroprotector effect on mice renal damage induced by Rhodamine B. Methods:This research was an experimental laboratory study with the posttest only controlled group design. Samples were 28 male mice, Swiss webster type, 2-3 months old and 20 g of each weight. Samples were divided into 4 groups of 7 mice each. This research used consecutive sampling. Strawberry juice was given for 16 days in a row, while Rhodamine B (0.2 ml/20 gr of mice body weight) was given on 10th to 16th day. Negative Control Group (KK-) was given distilled water only. Positive Control Group (KK+) was given Rhodamine B. First Treatmennt Group (KP1) and Second Treatment Group (KP2) were given 0.4 ml and 0.8 ml of Strawberry fruit juice per 20 gr of mice body weight and Rhodamine B. On the 17th day, mice were sacrificed by neck dislocation. After that, the kidney was dissected out and made for preparation with paraffin block method and stained with Hematoxilin Eosin (HE). The score of renal cells damage was determined by pyknosis, karyorrhexis, and karyolysis. The data were analyzed with One-Way ANOVA test (? = 0.05) and Least Significant Differences (LSD) test (? = 0.05). Results:The result of One-Way ANOVA test showed that there was a significant difference in score of renal cells (p < 0.05). LSD test showed a significant difference in score of renal cells (p < 0.05) across all pair groups. Conclusions:Strawberry (Fragaria x ananassa) fruit juice showed nephroprotector effect against renal cell histological damage in mice induced by Rhodamine B. Keywords:Strawberry fruit juice, Rhodamine B, renal cell histological damage.
The Effect of Soybean Powder (Glycine max) to the Histological Structure of Mices (Mus musculus) Liver Cells Induced by Used Cooking Oil Selvia Anggraeni; Endang Listyaningsih Suparyanti; Ratih Puspita Febrinasari
Nexus Kedokteran Translasional Vol 3, No 1 (2014): Nexus Kedokteran Translasional
Publisher : Fakultas Kedokteran Universitas Sebelas Maret Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.207 KB)

Abstract

Background: Soybean contain antioxidants which have hepatoprotective effect; one of them is isoflavone. Soybean powder has the highest isoflavone among soybean products. This research aims to prove that the use of soybean powder can prevent damage of the histological structure of the mices liver cells induced by used cooking oil. Methods: This study was an experimental laboratory with a post-test only control group design. Twenty eight Swiss Webster male mice were divided into 4 groups. Negative control group (KN) was given distilled water, the positive control (KP) was given 0.06 ml/ 20 g BW used cooking oil, dose 1 (KD1) was given soybean powder 5.6 mg/ 20 g BW and used cooking oil, and dose 2 (KD2) was given soybean powder 11.2 mg/ 20 g BW and used cooking oil. Soybean powder was given for 14 days, while the used cooking oil was given orally on 8th-14th day. On the 15th day, mice were sacrificed, taken their liver, and made ??preparations with HE staining. Liver cell damage was observed by counting the number of cells with pyknosis, karyorheksis, and karyolysis of 100 cells in liver centrolobular zone. Data were analyzed by statistical tests One-way ANOVA (Analysis of Variant) (? = 0.05). Results: The highest number of liver cells damage is on KP, followed by KD1, KD2, and at least on KN. Results of One-way ANOVA test showed significant differences among the four groups with p = 0.000 (p < 0.05). Results of Post Hoc Test of Multiple Comparasions using LSD test also showed significant differences between KN-KP, KN-KD1, KD2-KN, KP-KD1, KD2-KP, KD1-KD2 with respectively p = 0.000 (p < 0, 05). Conclusion: Soybean powder can prevent damage of the histological structure of the mices liver cells induced by used cooking oil. Keywords: soybean powder, used cooking oil, liver histological structure
The Influence of Broccoli Extract (Brassica oleracea var. Italica) to Liver Cells Damage in Mice (Mus musculus) Induced by Paracetamol Shinta Retno Wulandari; Endang Listyaningsih Suparyanti; Ratih Puspita Febrinasari
Nexus Biomedika Vol 5, No 2 (2016): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Introduction : Broccoli is known to contain antioxidants such as vitaminC, flavonoids and sulforaphane. Antioxidants can protect liver from damage caused by free radicals. This study aims to prove that broccoli extract protect liver cell from damage in mice induced by paracetamol in toxic dose and an increased doses of broccoli extract enhance the effects of protection to liver cell in mice. Methods : This study was an experimental laboratory with post test only controlled group design. Samples (30 mice) were taken by incidental sampling. Samples divided randomly into 5 groups, each group consisted of 6 mice. K1 was given distilled water. K2 was given paracetamol in toxic dose. KP1, KP2 and KP3 were given broccoli extract dose I, II and dose III respectively for 14 days and also were given paracetamol in toxic dose on day 12, 13 and 14. On day 15, the livers of mice were taken for making preparations with HE staining. The liver cell damages were observed by counting the nuclei of cells undergoing pyknosis, karyorrhexis and karyolysis. Data were analyzed by Oneway ANOVA and Post Hoc LSD analysis test (? = 0.05). Result : K2 had the most damaged cells (157.17 7.834 cells) and the least damaged cells were at K1 (1.50 1.049 cells). Oneway ANOVA test result was p = 0.00 (p < 0.05). Result of Post Hoc LSD analysis test showed a significant difference between groups on all pairs with p = 0.00 (p < 0.05). Conclusions : Broccoli extract protect liver cells from damage in mice induced by paracetamol. Increased doses of broccoli extract were proven to increase the protection effect on liver cells. Keywords: broccoli extract, paracetamol, liver cell damage
Co-Authors Dea Saufika Najmi Ratih Puspita Febrinasari Selvia Anggraeni Shinta Retno Wulandari Yulia Sari