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All Journal e-GIGI e-CliniC Jurnal e-Biomedik Jurnal Kedokteran Klinik Science Midwifery Medical Scope Journal (MSJ) Jurnal Impresi Indonesia Cerdika: Jurnal Ilmiah Indonesia
Heriyannis Homenta
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Religion Humanities Biochemistry, Genetics & Molecular Biology Dentistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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Journal : e-CliniC

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Identifikasi Pola Bakteri dan Uji Sensitivitas Antibiotik di Ruang Intensive Care Unit RSU GMIM Pancaran Kasih Manado Togas, Bellanty C.; Homenta, Heriyannis; Rares, Fredine E. S.
e-CliniC Vol. 12 No. 3 (2024): e-CliniC
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/ecl.v12i3.53546

Abstract

Abstract: Nosocomial infections or Healthcare Associated Infections (HAIs) are infections acquired during the process of receiving health care in hospitals, which appear within >48 hours. Patients in the Intensive Care Unit (ICU) are twice as likely to contract nosocomial infections compared to the general hospital population, with a fairly high and increasing burden of antimicrobial resistance. This study aimed to identify bacterial patterns and antibiotic sensitivity tests of bacteria in the ICU of RSU GMIM Pancaran Kasih Manado. This was a descriptive study with a cross sectional design. Bacterial pattern identification was performed on 10 samples, then samples were cultured in nutrient agar and Mac Conkey at the Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Sam Ratulangi. Identification of bacteria was conducted using the Gram stain. The results obtained Bacillus sp. (42%); Staphylococcus sp. (33%); Neisseria sp. (17%); and Staphylococcus aureus (8%). All bacteria were sensitive to amikacin, and nearly all of them were resistant to ceftriazone except Bacillus sp and Staphylococcus sp. In conclusion, the most common bacteria at the Intensive Care Unit of RSU GMIM Pancaran Kasih Manado is Bacillus sp. which is sensitive to amikacin and meropenem, but it is resistant to ampicilin. Keywords: bacterial pattern; nosocomial infection; antibiotic sensitivity test   Abstrak: Infeksi nosokomial (Healtcare Associated Infections - HAIs) adalah infeksi yang diperoleh selama proses menerima perawatan kesehatan di rumah sakit, yang muncul dalam waktu >48 jam. Pasien di Intensive Care Unit (ICU) dua kali lebih berisiko tertular infeksi nosokomial dibandingkan dengan populasi rumah sakit umumnya dengan beban resistensi antimikroba di ICU yang cukup tinggi dan terus meningkat. Penelitian ini bertujuan untuk mengidentifikasi pola bakteri yang ditemukan dan uji sensitivitas antibiotik pada bakteri di ruang ICU RSU GMIM Pancaran Kasih Manado. Penelitian ini menggunakan metode deskriptif dan desain potong lintang. Identifikasi pola bakteri dilakukan pada 10 sampel, kemudian dikultur menggunakan nutrient agar dan Mac Conkey di Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Sam Ratulangi, dan identifikasi bakteri dengan pewarnaan Gram. Hasil penelitian mendapatkan bakteri Bacillus sp. (42%); Staphylococcus sp. (33%); Neisseria sp. (17%); serta Staphylococcus aureus (8%). Semua bakteri sensitif terhadap antibiotik amikacin dan hampir semua bakteri resisten terhadap antibiotik ceftriazone kecuali Bacillus sp dan Staphylococcus sp. Simpulan penelitian ini yaitu bakteri terbanyak yang ditemukan di Ruang ICU ialah Bacillus sp. yang sensitif terhadap amikacin dan meropenem, namun resisten terhadap ampicilin. Kata kunci: pola bakteril infeksi nosokomiall; uji kepekaan antibiotik
Identifikasi Pola Bakteri dan Uji Sensitivitas Antibiotik di Kamar Bedah Mata Rumah Sakit Mata Provinsi Sulawesi Utara Benjamin, Estelina I.; Homenta, Heriyannis; Waworuntu, Olivia A.
e-CliniC Vol. 12 No. 3 (2024): e-CliniC
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/ecl.v12i3.53622

Abstract

Abstract: Healthcare-associated infections (HAIs) are infections of pathogenic microorganisms into the human body within 48-72 hours after a patient's hospital admission. These infections are commonly attributed to ventilator-associated pneumonia (VAP), surgical site infections (SSI), central line-associated bloodstream infections (CLABSI), and catheter-associated urinary tract infections (CAUTI). Increased HAI cases is also associated with an increase in Multiple Drug-Resistant (MDR) strains due to inappropriate antibiotic selection for treating diseases resulting from HAIs. This study aimed to identify bacterial patterns and sensitivity tests at the ophthalmic surgical room of Rumah Sakit Mata Provinsi Sulawesi Utara. Thus was an observational and descriptive study with a cross-sectional design. The results showed seven Bacillus sp., four Staphylococcus sp., and one Staphylococcus aureus. Sensitivity tests using 18 types of antibiotics revealed antibiotic resistance patterns of the identified bacteria: seven strains of XDR in Bacillus sp., four strains of MDR in Staphylococcus sp., and the presence of Methicillin-Resistant Staphylococcus aureus (MRSA). In conclusion, Bacillus sp. is the most abundant bacteria found. Bacillus sp. is sensitive to the antibiotic meropenem, while it is resistant to ceftriaxone and sulfamethoxazole/trimethoprim. Keywords: healthcare-associated infections; identification of bacteria; antibiotic sensitivity test    Abstrak: Healthcare-associated infections (HAIs) adalah infeksi mikroorganisme patogen ke dalam tubuh manusia yang terjadi 48-72 jam setelah pasien masuk rumah sakit, umumnya disebabkan oleh ventilator-related pneumonia (VAP), surgical site infections (SSI), central line associated blood stream infections (CLABSI), dan catheter-associated urinary tract infections (CAUTI). Peningkatan kasus HAIs sejalan dengan peningkatan multiple drug-resistant (MDR) karena ketidaktepatan pemilihan antibiotik dalam mengobati HAIs. Penelitian ini bertujuan untuk mengidentifikasi pola bakteri dan uji sensitivitas antibiotik di kamar bedah mata Rumah Sakit Mata Provinsi Sulawesi Utara. Jenis penelitian ialah observasional deskriptif dengan desain potong lintang. Hasil penelitian mendapatkan Bacillus sp. tujuh bakteri, Staphylococcus sp. empat bakteri, dan Staphylococcus aureus satu bakteri. Uji sensitivitas terhadap 18 antibiotik menunjukkan gambaran resistensi, yaitu: Bacillus sp. terdapat tujuh bakteri Extensively Drug-Resistant (XDR), Staphylococcus sp. terdapat empat bakteri MDR, dan Methicillin-Resistant Staphylococcus aureus (MRSA). Simpulan penelitian ini, yaitu: Bacillus sp., merupakan bakteri terbanyak yang ditemukan. Bacillus sp. sensitif terhadap antibiotik meropenem namun resisten terhadap antibiotik ceftriaxone, dan sulfamethoxazole/trimethoprim. Kata kunci: healthcare-associated infections; identifikasi bakteri; uji sensitivitas antibiotik
Identifikasi Pola Bakteri dan Uji Sensitivitas Antibiotik di Ruangan Instalasi Gawat Darurat RSU GMIM Pancaran Kasih Manado Sandjaya, Sulaiman F.; Waworuntu, Olivia A.; Homenta, Heriyannis
e-CliniC Vol. 12 No. 3 (2024): e-CliniC
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/ecl.v12i3.54050

Abstract

Abstract: Incidents of infection originating from hospitals can be referred to as Healthcare-Associated Infections (HAIs). Generally, hospitals have implemented policies and clinical practices in control of HAIs and the appropriate use of antibiotics to reduce the risk of infection spread and the development of antibiotic resistance. However, antibiotic administration in the Emergency Room (ER) significantly contributes to the excessive use of antibiotics, which may pose a problem of antibiotic resistance. This study aieds to determine the bacterial pattern and antibiotic sensitivity in the ER of RSU GMIM Pancaran Kasih Manado. This was an observational and descriptive study with a cross-sectional design. Identification of bacterial pattern and antibiotic sensitivity test on bacteria in the ER of RSU GMIM Pancaran Kasih Manado used the standard procedure in Microbiology Laboratory of Faculty of Medicine, Universitas Sam Ratulangi. The antibiotic sensitivity test for Bacillus sp. (53.84%), Staphylococcus sp. (38,46%), and Streptococcus sp. (7,69%) revealed the highest sensitivity to the antibiotics meropenem and gentamicin, while the antibiotics ampicillin/sulbactam and vancomycin showed the highest resistance levels. In conclusion, Gram-positive bacteria dominates the bacteria in the emerfency room of RSU GMIM Pancaran Kasih Manado, with Bacillus sp. being the most prevalent. Keywords: bacterial pattern; antibiotic sensitivity test; emergency room   Abstrak: Kejadian infeksi yang berasal dari rumah sakit termasuk dalam Healthcare-Associated Infections (HAIs). Rumah sakit telah menerapkan kebijakan dan praktik klinis dalam pengendalian HAIs dan penggunaan antibiotik yang tepat untuk mengurangi risiko penyebaran infeksi dan perkembangan resistansi antibiotik. Namun, ditemukan bahwa pemberian antibiotik di Instalasi Gawat Darurat (IGD) secara signifikan berkontribusi terhadap penggunaan berlebihan dari obat antibiotik yang kemungkinan menimbulkan masalah resistansi. Penelitian ini bertujuan untuk mengidentifikasi pola bakteri dan sensitivitas antibiotik di ruangan IGD RSU GMIM Pancaran Kasih Manado. Jenis penelitian ialah deskriptif observational dengan desain potong lintang. Identifikasi pola bakteri dan uji sensitivitas pada bakteri di ruangan IGD RSU GMIM Pancaran Kasih Manado menggunakan prosedur standar di Laboratorium FK Unsrat. Hasil identifikasi diperoleh bakteri Bacillus sp., Staphylococcus sp., dan Streptococcus sp. Uji sensitivitas antibiotik dari bakteri Bacillus sp. (53,84%), Staphylococcus sp. (38,46%), serta Streptococcus sp. (7,69%), mendapatkan tingkat sensitivitas tertinggi ditunjukkan terhadap antibiotik meropenem dan gentamicin, sedangkan hasil antibiotik ampicillin/sulbactam dan vancomycin memiliki tingkat resistensi tertinggi. Simpulan penelitian ini ialah bakteri Gram positif mendominasi bakteri di ruangan IGD RSU GMIM Pancaran Kasih Manado, dengan ditemukannya Bacillus sp. terbanyak. Kata kunci: pola bakteri; uji sensitivitas antibiotik; Instalasi Gawat Darurat
Persepsi Mahasiswa terhadap Objective Structured Clinical Examination (OSCE) Fakultas Kedokteran Universitas Sam Ratulangi 2023 Dolot, Jecky F.; Wungouw, Herlina I. S.; Homenta, Heriyannis
e-CliniC Vol. 12 No. 3 (2024): e-CliniC
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35790/ecl.v12i3.54913

Abstract

Abstract: Objective Structured Clinical Examination (OSCE) is a case scenario-based assessment method expected to be carried out by medical students as a benchmark for their future professional readiness. This study aimed to identify medical students' perceptions about OSCE. This was a retrospective and descriptive study with a cross-sectional design, employing a questionnaire designed by Fisseha and Desalegn. The results showed that based on a total of 85 students who took the UKMPPD OSCE exam in August 2023, 71 respondents (87.65%) completed the questionnaire. Students expressed that the OSCE exam was one of the triggers for stress. The OSCE exam structure received the highest score in the statement regarding students' readiness to take the OSCE exam. Organization of OSCE exam also received the highest score in the statement related to the conducive location of the OSCE exam, free from disturbances. The validity and reliability of OSCE exam were rated highest in the statement that the conduct of the OSCE exam was considered fair, without regard to ethnicity, race, culture, and gender. In conclusion, students' perceptions of the OCSE exam are considered good, covering characteristics, structure, management, validity, and reliability of the OSCE exam. The implementation of the OSCE exam has been standardized according to the OSCE exam implementation standards. Keywords: student perception; OSCE examination; medical education   Abstrak: Objective Structured Clinical Examination (OSCE) merupakan salah satu metode penilaian berbasis skenario kasus yang diharapkan dapat dikerjakan oleh mahasiswa rumpun kesehatan sebagai tolok ukur kesiapan profesi, yang bertujuan untuk menguji mahasiswa dari segi keterampilan komuni-kasi, pengetahuan klinis serta keterampilan klinis lainnya. Diperlukan evaluasi bagi setiap metode penilaian pendidikan kedokteran, baik dari tenaga pendidik maupun peserta didik. Penelitian ini bertujuan untuk mengidentifikasi persepsi mahasiswa Fakultas Kedokteran terhadap ujian OSCE. Jenis penelitian ialah deskriptif retrospektif dengan desain potong lintang, menggunakan kuesioner yang didesain oleh Fisseha dan Desalegn. Hasil penelitian memperlihatkan bahwa dari total 85 mahasiswa yang mengikuti ujian UKMPPD OSCE periode agustus 2023, didapatkan sebanyak 71 (87,65%) responden yang mengisi kuesioner. Mahasiswa berpendapat ujian OSCE salah satu pemicu stres, struktur ujian OSCE baik dengan pernyataan skor terbanyak yaitu kesiapan mahasiswa mengikuti ujian OSCE, pengelolaan ujian OSCE baik dengan pernyataan skor terbanyak yaitu lokasi pelaksanaan ujian OSCE kondusif, dan bebas dari gangguan, validitas dan reliabilitas ujian OSCE baik dengan pernyataan skor terbanyak yaitu pelaksanaan ujian OSCE dinilai adil, tanpa memandang suku, ras, budaya, dan jenis kelamin. Simpulan penelitian ini ialah persepsi mahasiswa terhadap ujian OCSE dinilai baik meliputi karakteristik, struktur, pengelolaan, validitas dan reliabilitas ujian OSCE. Pelaksanaan ujian OSCE sudah terstandarisasi sesuai dengan standar pelaksanaan ujian OSCE. Kata kunci: persepsi mahasiswa; ujian OSCE; pendidikan kedokteran
Co-Authors Alouw, Christy Natalia Arlita F. Sofyan Beatrix A. Lumentut, Beatrix A. Benjamin, Estelina I. Berhimpon, Siemona L. E. Bujung, Anggriana H. Dolot, Jecky F. Elmadany, Mujahida S Erling David Kaunang Fredine Rares Gabriella F.A. Kambey, Gabriella F.A. Gobel, Sitti N. Herlina I. S. Wungouw Johanna A. Khoman Manoppo, Firginia P. Maykel Sondak, Maykel Michael A. Leman Mohamad, Ramalia P. Nur Dinah Mamonto Olivia Waworuntu Pomandia, Natasha M. Pontororing, Aveva V. Porotu'o, John Porotu’o, John Porotuo, John Porotuo, John Porutu'o, John Prilly V. Londok Sandjaya, Sulaiman F. Sarlin, Hilary G. Sekeon, Helen N. Silvana Rares, Fredine Esther Standy Soeliongan Tesalonika Palit, Preisilia Tindas, Kezia A. Togas, Bellanty C. Tompodung, Linda Maya Ume, Eka Julistri Velma Buntuan Waani, Gebriela Naomi Waworuntu, Marshall C. Waworuntu, Patrick Johanes Winengko, Livia C. Yobee, Fransiska E.A.