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All Journal JURNAL ILMU DAN TEKNOLOGI PETERNAKAN Prosiding Animal Production : Indonesian Journal of Animal Production ANGON: Journal of Animal Science and Technology Farmers: Journal of Community Services
Titin Widiyastuti
Faculty Of Animal Science, Jenderal Soedirman University, Purwokerto
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Social Sciences Veterinary Other

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THE USAGE OF FERMENTABLE CARBOHYDRATES AND LEVEL OF LACTIC ACID BACTERIA ON PHYSICAL AND CHEMICAL CHARACTERISTICTS OF SILAGE Hidayat, Nur; Widiyastuti, Titin; , Suwarno
Prosiding Vol 3, No 1 (2012)
Publisher : Prosiding

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Abstract

Tujuan penelitian ini adalah untuk mengetahui pengaruh penggunaan tetes, katul dan onggok serta level bakteri asam laktat terhadap kandungan protein kasar, serat kasar, dan gross energi serta tekstur, pH, dan suhu silase rumput gajah. Penelitian ini menggunakan rancangan acak lengkap dengan Sembilan perlakuan dan tiga ulangan. Hasil analisis ragam menunjukkan bahwa perlakuan berpengaruh sangat nyata (P0,05) terhadap tekstur silase. Kesimpulannya adalah pada pembuatan silase rumput gajah (kadar air ± 60 %) penggunaan tetes 2 % baik dengan ataupun tanpa tambahan bakteri asam laktat lebih baik dibanding penggunaan 10 % katul dan 10 % onggok ditinjau dari karakteristik fisik dan kandungan nutrisinya.
Amino Acid and Mineral Supplementation in Fermentation Process of Concentrate Protein of Jatropha Seed Cake (Jatropha curcas L.) Widiyastuti, Titin; Sutardi, Tri Rahardjo
ANIMAL PRODUCTION Vol 18, No 3 (2016): September 2016
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (695.496 KB) | DOI: 10.20884/1.anprod.2016.18.3.574

Abstract

The purpose of this study is to assess the optimization of fermentation process by adding a minerals and amino acids so that the potential of protein  of Concentrate Protein-Jatropha seed cake (CP-JSC) can be optimally used as a substitute for soybean meal. The method used was completely randomized design. The treatment consisted of F1: Fermentation CP-BBJ + methionine-lysine (0.25%: 0.25%), F2: Fermentation CP-JSC + methionine-lysine (0.5%: 0.5%), F3: F1 + 0.45% Dicalsium Phosphate, F4: F2 + 0.45% Dicalsium Phosphate. Each treatment was repeated four times, When treatment significantly continued by Least Significant Difference (LSD), variables observed are the levels of antinutrients (phorbolester, antitrypsin), the levels of nutrients (fat, protein, crude fiber, Ca, P and gross energy) and amino acid. Results of analysis of variance showed that the addition of amino acids and minerals Ca, P in the fermentation process was highly significant effect on the levels of crude fiber and phosphorus (P <0.01) and significantly affected the gross energy content of CP-JSC post-fermentation (P <0.05). Dry matter, crude protein, crude lipid and calcium  are not affected by supplementation of methionine and lysine as well as calcium and phosphorus. Supplementation of methionine and lysine in the fermentation substrate showed good levels of essential amino acids and non essential higher than previous studies although not statistically significant (P> 0.05). While the levels obtained phorbolester range of 0.055% - 0.08%. It was concluded that the optimization of fermentation can be done without adding the amino acid supplementation of minerals calcium and phosphorus. Supplementation significantly affect a significant increase or decrease in some nutrients (crude fiber, gross energy, phosphor) and capable of suppressing a decrease in amino acids. Supplementation of amino acids Lysine and Methionin 0.05% is the best treatment.
Utilization of Pseudomonas fluorescens as Antimicrobial agent on Lipolysis Inhibitition of Rice Bran Widiyastuti, Titin; Prayitno, Caribu Hadi; Munasik, Munasik
ANIMAL PRODUCTION Vol 4, No 2 (2002): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Feedstuff was limited by fat content, certainly, if the material feedstuff was storage on long time periods. Fat was oxidized or hydrolyzed, which was decreased nutritional quality. A Research on Inhibited of  Lypolysis Rice Bran with anti-microbial compound of Pseudomonas fluorescens was conducted during ten month. The research used experiment methods, with Randomized Completely Block Design, storage as block and concentration of anti-mikrobial compound as treatment. Fat and polyunsaturated fatty acids content during storage was observed. Result of experiment showed anti-microbial compound inhibited oxidize and hydrolyze process of fat rice bran during six month storage. (Animal Production 4(2): 89-93 (2002) 
Fat and Cholesterol Content of Breast and Thigh Meat of Local Fowls Ismoyowati, Ismoyowati; Widiyastuti, Titin
ANIMAL PRODUCTION Vol 5, No 2 (2003): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The research was carried out to evaluate the influence of breed on fat and cholesterol content of breast and thigh meat of local fowls.  Tree breeds of local fowl consist of six male kampong chickens, Tegal ducks and muscovy ducks were applied.  An experimental method was used in this research.  Nested design was used.  The breeds of local fowl were as treatment, the part of carcass was as sub treatment and sample was as replication.  The result of the experiment showed that the part of carcass (breast and thigh) influenced meat fat and cholesterol content.  Breast meat had higher fat and cholesterol content than thigh meat.  Meat fat content had positive correlation with meat cholesterol. (Animal Production 5(2): 79-82 (2003) Key Words: Kampung Chicken, Duck, Muscovy, Breast, Thigh, Meat Fat and Meat Cholesterol
Nutrient Content of Napier Grass (Pennisetum purpureum) Silage Made with Various Additive and Modified Atmosphere in The Silo Widiyastuti, Titin; Hidayat, Nur; Indrasanti, Diana
ANIMAL PRODUCTION Vol 16, No 1 (2014): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Abstract. During ensilage, anaerob condition must be controlled. Some methods of modified atmosphere in silo were analyzed to compare ensilage characteristics and silage product. So far, there is not been information on the atmosphere condition in the process of silage production. It encourages the researchers to evaluate the condition of ensilage process of Pennisetum purpureum by studying atmosphere modification in the silo and the effect of the usage of various additives in the process of silage production.   Elephant grass (Pennisetum purpureum), molasses, L. acidophillus were used. The study was conducted with a Completely Randomized Design (CRD) 3x2 factorial pattern.  Atmosphere modification as the first factor consist of : (A0: silage with compaction (conventional) A1: silage with vacuum method, A2: silage with modified C02) and two kinds of silage additives as the second factor (B1: indirect additive (molasses); B2: direct additive (Lactic Acid Bacteria).  Each treatment combination was repeated 4 times. The objective of the research was to evaluate changes in nutrient content (protein, crude fiber, gross energy). The data obtained were analyzed by analysis of variance, then continued by Honest Significant Differences (HSD) test.  Based on the research results it can be concluded that the optimum ensilage can take place, either by compaction methods (conventional), vacuum and the addition of CO2. While the addition of molasses additive produces silage with better quality than the addition of L. Acidophillus inoculant. Key words: Modified atmosphere, additives, silage Abstrak. Kondisi anaerob harus dikontrol selama ensilase. Beberapa metode modifikasi atmosfir silo dianalisa untuk membandingkan ciri dan hasil ensilase. Sejauh ini belum ada informasi kondisi atmosfir dalam proses produksi ensilase. Hal ini mendorong peneliti untuk mengevaluasi kondisi proses ensilase Pennisetum purpureum dengan mempelajari modifikasi atmosfir silo dan dampak penggunaan bermacam zat tambahan dalam proses pembuatan ensilase. Rumput gajah (Pennisetum purpureum), molase, L. acidophillus digunakan dalam penelitian dengan Rancangan Acak Lengkap (RAL)  3x2 pola  faktorial. Modifikasi atmosfir sebagai faktor pertama terdiri dari: (A0: silase dengan pemadatan (konvensional), A1: silase dengan metode vakum, A2: silase dengan modifikasi CO2) dan dua jenis silase tambahan sebagai faktor kedua: B1: tambahan langsung (molase); B2: tambahan langsung (Bakteri Asam Laktat). Setiap kombinasi perlakuan diulang 4 kali. Tujuan penelitian adalah mengevaluasi perubahan dalam kandungan nutrisi (protein, serat kasar, energi kasar). Data yang diperoleh dianalisis dengan analisis variansi, dilanjutkan uji Beda Nyata Jujur (BNJ). Kesimpulan penelitian adalah ensilase optimal tercapai baik dengan metode pemadatan (konvensional), vakum, maupun penambahan CO2. Sedangkan tambahan molase menghasilkan silase dengan kualitas yang lebih baik daripada dengan  L. Acidophillus inoculant. Kata kunci: modifikasi atmosfir, zat tambahan, silase
Forage Business at Breed Source Area of Ruminansia in Central Java Province Muatip, Krismiwati; Widiyastuti, Titin; Hidayat, Nunung Noor; Purwaningsih, Hermin; Purwanto, Eko; Setya, Ghanis Ginanjar
ANIMAL PRODUCTION Vol 19, No 2 (2017)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (720.426 KB) | DOI: 10.20884/1.jap.2017.19.2.609

Abstract

Kebumen district is one of beef development centers in Central Java that requires numerous forage supplies (HPT). Farmers without personal foraging area have to buy forages almost on a daily basis. Forage business is an emerging and prospective business sector to improve farmers’ welfare particularly the income. Government is therefore expected to support in terms of facility and infrastructure to maintain a sustainable business practice.  The aim of this study was to investigate the necessity of forage business in cattle development center in Kebumen district, forage marketing channel, forage business revenue, profit margin, R/C ratio and government support in terms of infrastructure or policy related to forage business.
Optimization on Fermentation Process of Protein Concentrate of Jatropha Seed Cake with N Sources and Minerals Supplementation Widyastuti, Titin; Hidayat, Nur
ANIMAL PRODUCTION Vol 19, No 3 (2017)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (941.279 KB) | DOI: 10.20884/1.jap.2017.19.3.610

Abstract

The objective of this research is to produce alternative food sources of protein by optimizing the potential of jatropha curcas which is agroindustry waste. This study is planned in two years and is a series of jatropha seed exploration through fermentation using Lactobacillus acidophilus. Specific targets in the first year of study were to assess the optimization of the fermentation process by supplementing the source of N soybean meal and fish meal. Experiments using Completely Randomized Design (RAL) factorial pattern with first factor was supplementation (F) and second factor was incubation time (W), fermentation optimization consisted of: F1 (F0 + 2.5% soybean meal flour), F2 (F0 + 2.5% fish meal), F3 (F1 + 0.45% Dicalsium Phosphat) and F4 (F2 + 0.45% Dicalsium Phosphat). The incubation time is differentiated W1: 3 days, W2: 5 days and W3: 7 days. It can be concluded that: dry matter, gross energy, calcium and phospor are influenced by interaction between type of supplementation of source of N + DCP with fermentation time, whereas fat content is only influenced by fermentation time with optimal time decrease of fat content is 5,92 days. Total protein and amino acid levels are only influenced by different types of supplementation. Phorbolester antinutrition levels are influenced by the duration of the fermentation.  Based on antinutritive as a limiting factor. F4W5 is the best treatment and can used as a feed ingredient.
KADAR NDF DAN ADF KULIT BUAH KAKAO YANG DIFERMENTASI SECARA BERTINGKAT MENGGUNAKAN Trichoderma viride DAN Saccharomyces cerevisiae (NDF and ADF Levels of Cocoa Pod Husk Gradually Fermented Using Trichoderma viride and Saccharomyces cerevisiae) Andrik Lesmana; Ning Iriyanti; Titin Widiyastuti
Jurnal Ilmu dan Teknologi Peternakan Vol. 8 No. 2 (2020)
Publisher : Fakultas Peternakan, Universitas Hasanuddin, Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jitp.v8i2.6825

Abstract

The research aimed to examine the effect of gradual fermentation using Trichoderma viride and Saccharomyces cerevisiae and its most optimal level of use on lowering NDF and ADF levels of cocoa pod husks. The experiment was conducted experimentally according to a completely randomized design (CRD) consisted of four treatments and five replications. The treatments were  R0: cocoa pod husks without fermentation, R1: cocoa pod husk fermentation (T. viride 4% and S. cerevisiae 4%), R2: cocoa pod husk fermentation (T. viride 8% and S. cerevisiae 8%), R3: cocoa pod husk fermentation (T. viride 12% and S. cerevisiae 12%). Data were analyzed using analysis of variance (ANOVA) with the orthogonal polynomial test. The results showed that the gradual fermentation using T. viride and S. cerevisiae was highly significant in reducing levels of NDF and ADF of cocoa pod husks, following  the equation Y = 78.926 – 0.087X – 0.092 X2 + 0.007 X3,  (R2) = 93.4% and Y = 75.274 – 5.698X + 1.277 X2 – 0.073 X3, (R2) = 99.5%, respectively. In conclusion, the optimal level of using T. viride and S. cerevisiae in lowering the levels of NDF of cocoa pod husk was 9.21%, which can reduce the NDF level as much as 3.98%, while the optimal level of using  T. viride and S. cerevisiae in lowering the levels of NDF of cocoa pod husks was 3.01%, which can decrease the ADF level as much as 10.01%.
The Study of Protein Hydrolysis and Peptide Antioxidants Activity of Chicken Slaughterhouse Waste and Its Potential for Feed Additives Bambang Hartoyo; Titin Widyastuti; Sri Rahayu; Raden Singgih Sugeng Santoso
ANIMAL PRODUCTION Vol. 24 No. 2 (2022)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2022.24.2.133

Abstract

Ensuring food safety in livestock requires specific feeding technology in agriculture by using feed additives in the form of antibiotics, prebiotics, probiotics, acidifiers, hormones and enzymes. Bioactive peptides improve the health status of humans and animals. Protein hydrolysis produce peptides that are safe, quickly metabolizable, less risky for livestock products to have contaminant residue. Bioactive peptides are still bound to the original protein, so they need to be released through an enzymatic process. This research explored the potential of chicken slaughterhouse waste to produce biopeptides by hydrolyzing proteins using various proteolytic enzymes. The slaughterhouse waste included chicken feet, intestines, filleting waste and blood plasma. The proteolytic enzymes used were papain, bromelain, protease by Rhizopus oligosphorus, probiotic protease. The observed variables were dissolved protein content with and without precipitation, protein hydrolyzate content, and the degree of enzyme hydrolysis. The research was conducted using exploratory methods. The results showed that the dissolved protein content in the chicken slaughterhouse waste protein concentrate was 1,585 mg/ml (feet), 2,361 mg/ml (intestines), 1,787 (filleting waste) and 2,372 mg/ml (blood plasma). Blood plasma protein concentrate showed the highest yield among other chicken slaughterhouse waste protein concentrates, namely 0.14 mg/ml (hydrolysis of papain), 0.18 mg/ml (hydrolysis of bromelain), 0.56 mg/ml (hydrolysis of R.oligosphorus protease) 0.68 mg/ml (hydrolysis of probiotic proteases). The highest degree of hydrolysis was shown in blood plasma hydrolyzates using probiotic protease enzymes, namely 28.72%. The highest antioxidant activity was 92.92% as observed in chicken feet protein hydrolyzate which was hydrolyzed using papain. Therefore, chicken feet, intestines and fillet waste can produce protein concentrates through precipitation using ammonium sulfate, and plasma using acetone. The highest protein concentration was in blood plasma protein which also produced the highest hydrolysis from hydrolyzing blood plasma proteins with hydrolyzed probiotic protease. The highest antioxidant activity was observed in chicken feet protein hydrolyzate which was hydrolyzed using papain enzyme and incubated for 6h.
Efek Suplementasi Tepung Rumput Laut Merah (Gracilaria sp.) Terhadap Kecernaan Bahan Kering Dan Kecernaan Bahan Organik Pakan Domba Secara In Vitro Adi Nugroho; Caribu Hadi Prayitno; Titin Widiyastuti
ANGON: Journal of Animal Science and Technology Vol 1 No 2 (2019): JURNAL ANGON
Publisher : Fakultas Peternakan Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (847.235 KB) | DOI: 10.20884/1.angon.2019.1.2.p122-128

Abstract

Background. The aims of this research were to determine the best level of Gracilaria sp. Seaweed meal based on its effect on dry matter digestibility and organic matter digestibility of sheep feed on in vitro. Materials and methods. The materials used were rumen fluid from 3 sheeps that were kept in Greenhouse of Faculty of Animal Science Jenderal Soedirman University had been adapted with the feed to be tested. The treatments were supplementation of Gracilaria sp. seaweed meal at the level of 0% as control feed, P0 + 2% (P1), P0 + 4% (P2), P0 + 6% (P3), dan P0 + 8% (P4) of dry matter feed on a ration composed of 40% concentrate and 60% Cynodon dactylon forage. The method used was the experiment method using a Completely Randomized Design (CRD).The each treatment was repeated 4 times, so there were 20 experimental units. The variables measured were dry matter digestibility and organic matter digestibility. Results. The results of dry matter digestibility were 65.8 ± 1.83% (P0); 63.42 ± 0.92% (P1), 66.29 ± 1.37% (P2), 69.35 ± 1.4% (P3) and 71.04 ± 2.44% (P4). The results of organic material digestibility were 63.96 ± 2.22% (P0); 60.17 ± 1.69% (P1), 65.69 ± 3.29% (P2), 68.33 ± 1.61% (P3) and 69.72 ± 2.77% (P4). The results showed that the treatment had highly significant effect (P <0.01) on the digestibility of dry matter and organic matter. The results showed that higher supplementation of seaweed meal (Gracilaria sp.) could increased digestibility (dry and organic matter). Conclusion. Based on these results it can be concluded that, supplementation of Gracilaria sp. seaweed meal at the level of 8% gives a significant effect on the dry matter digestibility and organic matter digestibility.
Co-Authors Adi Nugroho Adipatya Wisnuwardhana Ady Trimo Wijoyo Raharjo Agustinah Setyaningrum, Agustinah Andrik Lesmana Anton Budi Prasetyo Bambang Hartoyo Caribu Hadi Prayitno Caribu Hadi Prayitno Diana Indrasanti Eko Hendarto Eko Purwanto Elly Tugiyanti Fajar Arafadi FM. Suhartati Hermin Purwaningsih Herry Suprapto Ismoyowati Kasman Suherman Krismiwati Muatip Munasik Munasik Ning Iriyanti Nunung Noor Hidayat Nur Hidayat Nur Hidayat Prasetyo Prasetyo Raden Singgih Sugeng Santoso Setya, Ghanis Ginanjar Siska Nopitasari SRI RAHAYU Sri Rahayu Suparwi Suparwi Suwarno Tri Rahardjo Sutardi Utomo, Dzulfikar Satrio Wardhana Suryapratama Widyastuti, Titin