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All Journal HAYATI Journal of Biosciences Indonesian Journal of Biotechnology KLOROFIL: Jurnal Ilmu Biologi dan Terapan Jurnal Ilmu Sosial Politik dan Humaniora (Jisora) KLOROFIL: Jurnal Ilmu Biologi dan Terapan Current Research on Biosciences and Biotechnology Environmental and Materials
Husna Nugrahapraja
School of Life Sciences and Technology, Institut Teknologi Bandung, Jl. Ganesha No. 10, Bandung, 40132, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Control & Systems Engineering Earth & Planetary Sciences Energy Engineering Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Social Sciences

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Revealing disease‐specific endogenous target mimic of microRNA from long non‐coding RNA identification and characterization in Musa spp. Audie Masola Putra; Husna Nugrahapraja; Fenny Martha Dwivany
Indonesian Journal of Biotechnology Vol 28, No 1 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.49368

Abstract

Banana (Musa spp.) is one of the most widely consumed fruits in the world. Unfortunately, the plants are at risk from many disease problems, which mainly derive from microorganism. It is a little known about the relationship between disease‐inducing microorganisms and plants, particularly at the molecular level. This research aimed to characterize long non‐coding RNA (lncRNA) from bananas that may have roles in regulating gene expression related to the disease response mechanism in banana derived from transcriptomic libraries. Furthermore, the detected transcripts were analyzed to identify the endogenous target mimics (eTMs) interaction between lncRNA and microRNA (miRNA) using computational approaches. Data from Cavendish banana (AAA group), Berangan (AAA group), Yunnan Banana (Itinerans), Dajiao (ABB group), and Klutuk (BB group) were used in this research. We found that lncRNA tends to be unsustainable, and most sizes are below 1000 bp (≥ 75%). Based on this result, we investigated the eTMs to determine lncRNA transcripts and miRNA, such as miR397 in Cavendish and miR444 in Klutuk. This transcript would be regulated following exposure to extreme temperatures and disease, indicating the possibility of disease‐specific interaction between bananas and their environment at the molecular level.
Diversity analysis of moray eel (muraenidae) on artificial reef structure in mengiat beach, Nusa Dua, Bali Wilbert, Nathanael; Husna Nugrahapraja
Environmental and Materials Vol. 1 No. 1: (June) 2023
Publisher : Institute for Advanced Science, Social, and Sustainable Future

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61511/eam.v1i1.2023.62

Abstract

Optimal coral growth has a positive impact on increasing biodiversity. This can be observed through the increase in the number of marine species as the transplanted coral reefs age. One natural indicator in coastal ecosystems is the presence of moray eels. Within the coral reef ecosystem, the diversity of moray eels as natural predators belonging to the category of reef fish can serve as an indication of a healthy and balanced ecosystem. The method used in this research is visual counting or visual observation of several species of marine eels on three different major installed reef star media, as part of coral reef restoration efforts. The data collection locations include three points of reef star media installation: BTN, Coral Garden, and Cluster 5. The water conditions in these locations, such as salinity, temperature, pH, and conductivity, indicate the adequate quality to support coral reef restoration efforts. In this study, five species of marine eels frequently encountered in the three data collection locations identified as: Echidna nebulosa, Gymnothorax thyrsoideus, Gymnothorax fimbriatus, Gymnothorax pictus, and Gymnothorax richardsonii. The highest abundance and diversity were found in Coral Garden, followed by Cluster 5, with BTN being the lowest.
Study of the sea urchins (echinoidea) influence on the coral reef communities in the Nusa Dua Bali conservation area Angellia, Vonny; Husna Nugrahapraja
Environmental and Materials Vol. 1 No. 1: (June) 2023
Publisher : Institute for Advanced Science, Social, and Sustainable Future

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61511/eam.v1i1.2023.63

Abstract

Coral reef ecosystems are crucial for marine ecosystems as they support various other marine organisms. Sea urchins, such as Diadema setosum and Echinometra mathaei influence the coral reef communities by consuming algae that disrupt coral growth. This study aims to determine reef star coral media quantity, identify the dominant coral species at three points (BTN, Coral Garden, and Cluster 5) in Nusa Dua Beach, and assess the influence of sea urchins on coral reefs. The sampling method used was Purposive sampling, and visual observations which were conducted from June 27 to June 30, 2022. Results showed the varying sea urchin populations, with Acropora sp. and Pocillopora sp. dominating at BTN, Acropora sp. and Pectinia sp. at Coral Garden, and Acropora sp. at Cluster 5. The findings suggest that sea urchins play a key role in maintaining coral reefs by consuming macroalgae and supporting coral survival.
Co-Authors Adi Pancoro Adriana Hiariej, Adriana Agung Kurniawan Agus Sutanto Agus Sutanto Angellia, Vonny Annisa Ratna Nurillah Audie Masola Putra Aulia Marwah Mumtaza Bentang Arief Budiman Dadang Sumardi Dinda Syafira Edoardo Bertolini FENNY MARTHA DWIVANY Giasintha Stefani Hana Verdiani Hari Purnama I NYOMAN RAI Ima Mulyama Zainuddin Intan Fatmawati Karlia Meitha Ketut Wikantika Mario Enrico Pe Muhammad Abdur Rasyid Natas Setiabudhi Daryono Putra Natas Setiabudhi Daryono Putra Nathanael Wilbert Nisrina Sukriandi Raden Aswin Rahadi Rijanti Rahaju Maulani Rindia Maharani Putri Sigit Nur Pratama Sony Suhandono Sri Aswin Suryani Tati Suryati Syamsudin Topik Hidayat Vonny Angellia Wervyan Shalannanda Wilbert, Nathanael