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All Journal Medicina Sport and Fitness Journal E-Jurnal Medika Udayana WMJ (Warmadewa Medical Journal) Sari Pediatri Majalah Kesehatan FKUB ISM (Intisari Sains Medis) : Jurnal Kedokteran Neurologico Spinale Medico Chirurgico IJAAM (Indonesian Journal of Anti-Aging Medicine) Cermin Dunia Kedokteran
Ida Sri Iswari
Department Of Clinical Pathology, Faculty Of Medicine, Udayana University
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Social Sciences

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Surveilans Influeza pada Pasien Rawat Jalan Putu Siadi Purniti; Ida Bagus Subanada; Ida Sri Iswari
Sari Pediatri Vol 12, No 4 (2010)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.688 KB) | DOI: 10.14238/sp12.4.2010.278-82

Abstract

Latar belakang. Influenza merupakan infeksi yang sering terjadi dan memberikan dampak yang besar padakelompok usia anak. Informasi mengenai tingkat kunjungan pasien seasonal influenza diperlukan untukmenilai besarnya dampak yang ditimbulkan penyakit influenza Rapid influenza test dapat membantu diagnosisdan penanganan pasien yang datang dengan manifestasi klinis seasonal influenza.Tujuan. Untuk mengetahui tingkat kunjungan pasien dengan seasonal influenza dan nilai diagnostik pemeriksaanrapid influenza test.Metode. Surveilans seasonal influenza dilaksanakan di Poliklinik Rawat Jalan Bagian//SMF Ilmu KesehatanAnak RSUP Sanglah Denpasar Bali selama periode 1 Januari 2005 sampai 31 Desember 2006. Pasien usia1 bulan sampai dengan 12 tahun dengan manifestasi klinis influenza like illness berdasarkan kriteria WHOdiikutsertakan dalam penelitian. Pada subyek penelitian dilakukan apusan hidung untuk pemeriksaan rapid influenzadan apusan tenggorok untuk pemeriksaan reverse transcriptase polymerase chain reaction (RT-PCR).Hasil. Didapatkan prevalensi seasonal influenza pada anak di RSUP Sanglah Denpasar Bali 16,8%. Duapuncak kejadian terjadi pada periode bulan Januari sampai April dan September sampai Desember. Pemeriksaanrapid influenza memiliki sensitivitas 12,87% (IK 95%: 12,54-12,89%), spesifisitas 99,6% (IK 95%:99,59-99,61%), nilai duga positif 86,67% (IK 95%: 86,47-86,94%), nilai duga negatif 84,98% (IK 95%:82,18-87,78%), rasio kemungkinan positif 32,18% (IK 95%: 28,45-35,91%), dan rasio kemungkinannegatif 0,88% (IK 95%:62,1-113,8%).Kesimpulan. Prevalensi seasonal influenza pada penderita rawat jalan di Poliklinik anak RSUP SanglahDenpasar Bali 16,8%. Dua puncak kejadian terjadi pada periode bulan Januari–April dan September–Desember. Pemeriksaan rapid influenza memiliki sensitivitas yang rendah tetapi spesifisitas dan nilai dugapositif yang tinggi
Administration of Etanol White Turmeric (Curcuma zedoaria) Extracts Increased Superoxide Dismutase (SOD) And Decreased Malondialdehide (MDA) In Cigarette Smoke-Exposed Male Mice (Mus musculus) Strain Balb/C Yuliana Tjua; Ida Sri Iswari; I Gusti Made Aman
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 2 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i2.92

Abstract

Background: Cigarette smoke is free radical which decrease superoxide dismutase (SOD) and increased malondialdehyde (MDA) levels. Etanol white turmeric (Curcuma zedoaria) extract contains flavonoids, polyphenols, tannins, and antioxidant activity. This study aimed to prove white turmeric increased SOD and decreased MDA in cigarette smoke-exposed male mice (Mus musculus) strain Balb/c. Methods: This study used a randomized pre-test and post-test control group design. The subjects were 12 male mice and divided into two groups (n = 6). The control group was exposed to cigarette smoke 1 stick/day and given aquadest 1 ml as a placebo. The treatment group was exposed to cigarette smoke 1 stick/day and given white turmeric extract of 4 mg/20gram BW in one-hour cigarette smoke exposure. The blood was drawn through canthus medial sinus orbital to examine the level of SOD by ELISA and MDA level by TBARS method. Results: There was no significant differences in SOD levels (p = 0.930) and MDA levels (p= 0.984) between groups in pre-test. However, the mean SOD levels (p <0.001) and MDA levels (p<0.001) between groups were significant in post-test. In control group, there was no change in SOD (from 0.555±0.156 to 0.595±0.233 ng/mL; p = 0.650) and MDA (from 14.3±0.614 to 14.3±0.513 μM; p = 0.958). In contrast, the treatment group has increase SOD (from 0.565±0.273 to 2.28±0.110 ng/mL; p <0.001) and decrease MDA (from 14.3±0.931 to 5.78±1.06 μM; p <0.001). Conclusion: Administration of Etanol white turmeric extracts increased SOD and decreased MDA in cigarette smoke-exposed male mice strain Balb/c.
Oral administration of palm oil (elaeis) increases abdominal fat more than oral administration of coconut oil (cocos nucifera) in wistar male rats Cynthia Sofyanti Sugiharto; I Gusti Made Aman; Ida Sri Iswari
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 2 No 1 (2018): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (175.382 KB)

Abstract

Introduction: Coconut oils contain many medium-chained fatty acids (MCFA) which are quickly oxidized to produce more energy than long-chained fatty acids (LCFA) in palm oil which is to be accumulated as body fat. The purpose of this study was to determine that oral administration of palm oil (Elaeis) in wistar male rats increased abdominal subcutaneous fat and visceral fat compared to coconut oil (Cocos nucifera). Methods: An experimental post-test only control group study was conducted using 30 male Wistar rats, 2.5-3 months old, with body weight 130-135 grams, which then treated with distilled water in [Control group (P0)], palm oil [Treatment group 1] (P1)] and coconut oil [Treatment group 2 (P2)] with a dose of 2x0.4 ml daily for 45 days. After treatment, the rat was dissected and the visceral abdominal and subcutaneous fats were weighted. All data were analyzed by One Way ANOVA test. Result: According to post-test analysis, the mean weight of abdominal subcutaneous fat was found at 1.18 ± 0.27 grams, 1.60 ± 0.20 grams, and 0.90 ± 0.36 grams in groups P0, P1 and P2 respectively. The mean visceral fat weight was 0.88 ± 0.45 grams, 1.46 ± 0.34 grams, and 0.69 ± 0.35 grams in groups P0, P1 and P2 respectively. One Way ANOVA test analysis found that there were significant differences in mean weight of visceral fat and abdominal subcutaneous fat between the three groups (p <0.05). Conclusion: It was concluded that, with the same dose and timeframe, the administration of oral palm oil in Wistar male rats has more tendency to increase abdominal fat than coconut oil.
Topical administration of red ginger (Zingiber officinale Rosc) extract gel increased the number of fibroblast, neovascularization, and epitelization of wound healing in old male mice (Mus musculus) Hayyu Kusumaningtyas; I Gusti Made Aman; Ida Sri Iswari
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 5 No 1 (2021): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v5i1.72

Abstract

Introduction: Wound healing is slower with advancing age. Red ginger extract is a natural compound that contains flavonoids and polyphenols. This study aims to prove that the topical administration of red ginger (Zingiber officinale Rosc) extract gel increases the number of fibroblasts, neovascularization, and epithelialization of wound healing in old male mice (Mus musculus). Methods: This study was an experimental study using randomized posttest only control group design using 32 male mice, aged 12 months, weighing 20 grams, which was divided randomly into 2 groups. The control and treatment group was further divided into 4-day group and 8-day group. The control group was applied with placebo gel twice a day and given oral amoxicillin for three days (3 times a day) in the wound site, while the treatment group was applied with 30% red ginger extract gel twice a day and given oral amoxicillin. Results: The average number of neovascularization in the fourth day of the control group was 31.37±3.98, while in the treatment group was 68.17±11.56 (p< 0.001). The average number of fibroblasts on the fourth day of the control group was 77.04±5.57, and in the treatment group was 103.99±10.68 (p< 0.001). On the fourth day there was no epithelialization observed, and on the eight day the mean epithelialization in the control group observed was 30.35±6.01 μm, while in the treatment group was 78.25±18.26 μm (p<0,001). Conclusion: Topical administration of red ginger extract gel increased the number of fibroblasts, neovascularization, and epithelialization of wound healing in old male mice.
Administration of Acacia (Acacia mangium) Leaves Ethanol Extract Cream 5% Prevented The Increase of Melanin Number in Ultraviolet B-Exposed Guinea Pig (Cavia Porcellus) Skin Olivia Julita; Ida Sri Iswari; Anak Agung Gede Putra Wiraguna
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 4 No 2 (2020): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/ijaam.v4i2.101

Abstract

Introduction: Ultraviolet exposure stimulate oxidative stress-mediated melanogenesis, which is the sign of photoaged skin. Acacia (Acacia mangium) leaves extract contains high levels of antioxidants. The aim of this study was to prove the effectiveness of the Acacia leaves ethanol extract cream in preventing an increase of melanin. Methods: This experimental study used randomized posttest only control group design. The research subjects were 36 male guinea pigs (Cavia porcellus), healthy, local strains, aged 3 months, weighing 300-350 grams and had never received prior treatment. Subjects were divided into 2 groups (18 individuals each), namely the control group (treatment with ultraviolet B exposure and basic cream) and the treatment group (treatment with ultraviolet B exposure and acacia leaves ethanol extract cream 5%). After 2 weeks of treatment, the amount of melanin was examined histopathologically using Masson-Fontana staining. Results: The average number of melanin in the control group was 19.33±2.59% while in the treatment group was 2.21±1.25%. Comparative analysis using independent t-test showed that the value of p<0.001. Conclusion: This study suggesting that administration of acacia (Acacia mangium) leaves ethanol extract cream 5% prevented increasing amount of melanin in ultraviolet B-exposed guinea pig (Cavia porcellus) skin. The results showed that there was number of melanin in the control group (19.33±2.59%) were higher than the treatment group (2.21±1.25%) (P<0.001).
Ekstrak Teh Putih (Camellia sinensis) Oral Mencegah Dislipidemia pada Tikus (Rattus novergicus) Jantan Galur Wistar yang Diberi Diet Tinggi Lemak Delly Dahlia; Wimpie I Pangkahila; I Gusti Made Aman; J Alex Pangkahila; N. Tigeh Suryadhi; Ida Sri Iswari
IJAAM (Indonesian Journal of Anti-Aging Medicine) Vol 1 No 1 (2017): Indonesian Journal of Anti-Aging Medicine
Publisher : Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (745.119 KB)

Abstract

Dislipidemia adalah kelainan metabolisme lipid yang ditandai terjadinya peningkatan kolesterol total, trigliserida, kolesterol LDL dan penurunan kolesterol HDL. Pada saat ini banyak penelitian untuk mencegah dan mengobati dislipidemia dengan bahan alami. Teh putih merupakan teh tanpa proses fermentasi yang berasal dari daun teh (camellia sinensis) yang sangat muda dan masih menggulung serta terlindung dari sinar matahari sehingga mencegah degradasi polifenol. Ekstrak teh putih mengandung derivat katekin tertinggi dibanding teh lainnya , ECGC (Epigalocathecin 3-Gallate) dan kafein ini dapat memperbaiki profil lipid darah dan memiliki efek vasoprotektif, juga memiliki kemampuan untuk menginhibisi (Cholesteryl ester transfer protein) CETP, yang bisa meningkatkan kadar kolesterol HDL dan menurunkan kadar kolesterol total ,trigliserida dan kolesterol LDL. Penelitian ini bertujuan untuk mengetahui kegunaan Ekstrak Teh Putih (camellia sinensis) sebagai alternatif untuk mencegah dislipidemia dan mengetahui dosis pemberian ekstrak teh putih untuk mencegah dislipidemia pada tikus jantan galur wistar. Penelitian ini adalah penelitian eksperimental murni dengan randomized posttest only control group design. Tikus putih jantan dipilih secara random dan dibagi menjadi 3 kelompok, masing-masing berjumlah 10 ekor tikus, yaitu kelompok kontrol diberikan diet tinggi lemak dan plasebo yang berupa akuades , kelompok perlakuan I diberi diet tinggi lemak dan ekstrak teh putih 14,4 mg, dan kelompok perlakuan II diberi diet tinggi lemak dan ekstrak teh putih 28,8 mg masing-masing 1 kali sehari. Setelah perlakuan selama 28 hari sampel darah diambil dari medial kantus sinus orbitalis, untuk pemeriksaan kadar kolesterol total, trigliserida, kolesterol LDL dan kolesterol HDL. Hasil penelitian menunjukkan bahwa pada kelompok pemberian ekstrak teh putih 14,4 mg dan pemberian ekstrak teh putih 28,8 mg terdapat penurunan kolesterol total secara bermakna masing-masing 137,31% dan 156,65% (p < 0,05), penurunan trigliserida secara bermakna masing-masing 77,29% dan 101,01%(p < 0.05) dan penurunan kolesterol LDL masing-masing 53,58 % dan 75,12%(p < 0,05), serta peningkatan kolesterol HDL secara bermakna masing-masing 44,31% dan 66,39% (p < 0,05). Penelitian ini menyimpulkan ekstrak teh putih mencegah peningkatan kolesterol total, trigliserida, kolesterol LDL dan mencegah penurunan kolesterol HDL, jadi ekstrak teh putih mencegah dislipidemia.
Terminalia ferdinandiana inhibited the increased of matrix metalloproteinase-1 and prevent collagen decreased in mice skin exposed to UV-B Shalihaty Emy; Ida Sri Iswari; Ni Wayan Winarti
Neurologico Spinale Medico Chirurgico Vol 4 No 1 (2021)
Publisher : Indoscholar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36444/nsmc.v4i1.136

Abstract

Background: Kakadu cream contained 0,1% Kakadu plum (Terminalia ferdinandiana) extract with a high content of phenolic compound, flavonol, tocopherol, luthein, chlorophyll, and ellagic acid has potential to prevent the Ultraviolet-B (UV-B) effect on skin aging acceleration. To date, there’s no in vivo experiment on the effect of kakadu plum extract on collagen nor matrix metalloproteinase-1 (MMP-1). This research aims to evaluate the effectiveness of Kakadu cream administration in inhibiting the increase of MMP-1 expressions and prevent the decrease of collagen amount in mice (Mus musculus) skin exposed to UV-B. Methods: An experimental study with a post-test only control group design was employed in 36-male-mice, 6-8 weeks old, weighing 20-25 grams. The samples were divided randomly into two groups, a control group given base cream and the study group, given Kakadu cream 0,1% on their shaved backs, 1 cm2 in size as the UV-B exposure's location. The UV-B irradiation was done three times a week for 4-weeks. The base and Kakadu cream were given twice a day. Comparative analysis was carried out to compare MMP-1 expression and collagen amount in both groups. Results: The results show that the mean of MMP-1 expression on the study group was significantly lower compared to control group (p<0.001). The mean collagen amount was significantly higher in the study group than in the control group (p< 0,001). Conclusion: From the results, can be concluded that Kakadu cream inhibited the increase MMP-1 expression and prevent the decrease of collagen amount in mice skin exposed to UV-B.
Eucalyptus globulus essential oil increased neovascularization and vascular endothelial growth factor expression in male wistar rats exposed to UVB Noviana Indrawati; Ida Sri Iswari; Ni Wayan Winarti
Neurologico Spinale Medico Chirurgico Vol 4 No 3 (2021)
Publisher : Indoscholar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36444/nsmc.v4i3.187

Abstract

Background: Eucalyptus globulus essential oil contained 1,8-cineol, which may accelerate wound healing. This study aimed to prove Eucalyptus globulus essential oil's effect on increasing neovascularization and Vascular Endothelial Growth Factor (VEGF) expression in Wistar rats skin exposed to ultraviolet B (UVB). Methods: A Post-test only controls group study design was applied on 36 male Wistar rats, aged 3-4 months old, weighing 150-200 grams. The animal subject was exposed to UVB (65 mJ/cm2, 65 seconds for seven days), injured on their back using a punch biopsy with a diameter of 10 mm, and divided into two groups. For seven days, on the wound, the control group was given 2 cc of 0.9% NaCl twice a day, while the treatment group was given Eucalyptus globulus essential oil (2 cc). Both groups were given oral Amoxicillin three times a day. The skin tissue was taken on day eight to assess neovascularization and VEGF expression. Results: The results show that neovascularization in the treatment group was higher than in the control group (p < 0.05). Meanwhile, the median VEGF expression in the treatment group was also higher than in the control group (p < 0.05). Conclusions: Topical administration of Eucalyptus globulus essential oil increased neovascularization and VEGF expression in the skin of male Wistar rats injured and exposed to UVB. Further research is needed to assess the effectiveness of the essential oil in enhancing neovascularization and VEGF expression in humans with multiple exposures at different doses to optimize the wound-healing effect.
Prevalensi gen penyandi resisten Colistin mcr-1 pada isolat Klebsiella pneumoniae dan Escherichia coli Extended-Spectrum Beta-Lactamases (ESBL) di RSUP Sanglah, Bali tahun 2018 Gusti Ayu Dianti Violentina; Ni Nyoman Sri Budayanti; Ida Sri Iswari
Intisari Sains Medis Vol. 11 No. 3 (2020): (Available online: 1 December 2020)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.734 KB) | DOI: 10.15562/ism.v11i3.820

Abstract

Background: Antimicrobial resistance (AMR) is a serious threat to health, both human and animal health. Antibiotic resistance in the Enterobacteriaceae family, especially the bacteria Klebsiella pneumoniae and Escherichia coli, is a special focus because it is caused by pathogenic Gram-negative bacteria found in humans. This study aims to study the prevalence of Colistin mcr-1 resistant coding gene in Klebsiella pneumoniae and Escherichia coli Extended-Spectrum Beta-Lactamases (ESBL) isolates in Sanglah Hospital, Bali, in 2018.Methods: This study was conducted in 2 stages, namely examination by PCR and telephone by sequencing. This study's object was the bacteria Klebsiella pneumoniae and Escherichia coli ESBL in clinical isolates of the Clinical Microbiology Laboratory of Sanglah Hospital Denpasar-Bali. Data were analyzed using SPSS version 20 for Windows.Results: The results of this study indicate that the results of clinical examination of 300 isolates, most of the K. pneumoniae and E. coli specimens were found in urine (32.00%), followed by pus (24.00%), sputum (20.00%), blood (12.00%), and others (11.00%). Most of the K. pneumoniae infections were found in sputum (70.49%), pus (52.05%), and blood (70.27%) specimens. Of the 300 isolates obtained from the results, no isolates had the gene encoding colistin mcr-1 resistance in clinical isolates tested molecularly.Conclusion: From the results of this study, it can be concluded that the colistin mcr-1 resistance coding gene was not found in the tested isolates.  Latar Belakang: Antimicrobial resistance (AMR) merupakan ancaman serius di dunia kesehatan baik kesehatan manusia maupun hewan. Resistensi antibiotika pada family Enterobacteriaceae khususnya bakteri Klebsiella pneumoniae dan Escherichia coli menjadi fokus khusus karena keduanya merupakan bakteri Gram negatif patogen yang sering ditemukan pada manusia. Penelitian ini bertujuan untuk mengevaluasi prevalensi gen penyandi resisten Colistin mcr-1 pada isolat Klebsiella pneumoniae dan Escherichia coli Extended-Spectrum Beta-Lactamases (ESBL) di RSUP Sanglah, Bali tahun 2018Metode: Penelitian ini dilakukan dalam 2 tahap yaitu identifikasi dengan PCR dan identifikasi dengan sekuensing. Objek dari penelitian ini ada bakteri Klebsiella pneumoniae dan Escherichia coli ESBL pada isolat klinik Laboratorium Mikrobiologi Klinik RSUP Sanglah Denpasar-Bali. Data dianalisis dengan SPSS versi 20 untuk Windows.Hasil: Hasil penelitian ini menunjukkan bahwa hasil pemeriksaan terhadap 300 isolat klinis sebagian besar specimen K. pneumoniae dan E. coli ditemukan pada urin (32,00%), diikuti dengan pus (24,00%), sputum (20,00%), darah (12,00%), dan lain-lain (11,00%). Sebagian besar infeksi K. pneumoniae ditemukan pada spesimen sputum (70,49%), pus (52,05%), dan darah (70,27%). Dari 300 isolat yang diperiksa didapatkan hasil bahwa tidak ada isolat yang memiliki gen penyandi resistensi colistin mcr-1 pada isolat klinik yang diujikan secara molekuler.Kesimpulan: Dari hasil penelitian ini dapat disimpulkan bahwa gen penyandi resisten colistin mcr-1 tidak ditemukan pada isolat yang diuji
Kesesuaian pewarnaan gram dengan kultur darah sebagai prediktor nilai kritis kasus bakteremia di Rumah Sakit Umum Pusat Sanglah, Denpasar Putu Yoska Arya Harindana; Ida Sri Iswari; Indramawan Setyojatmiko; Ni Nengah Dwi Fatmawati
Intisari Sains Medis Vol. 12 No. 2 (2021): (Available Online: 1 August 2021)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (481.58 KB) | DOI: 10.15562/ism.v12i2.1038

Abstract

Background: One of the considerations for giving empiric antibiotics in bacteremia cases is gram staining (GS) results. Accurate and fast results are required in distinguishing infection-caused bacteria. However, the data on how much the corresponding gram stain results with bacterial growth in blood cultures are still insufficient.Aim: The study aims to compare Gram stain results with bacterial growth in positive blood cultures.Methods: A cross-sectional analytic study obtained data from the VITEK® 2 Compact (bioMérieux) results for six months (January - June 2020). Data involved all blood cultures examined as many as 509.Results: Of the 509 blood samples, 46 were reported as critical values for bacteremia. Gram-negative bacillus bacteria were identified in 39.13% of the gram staining (GS) and 45.65% of the blood culture (BC) samples. Gram-positive bacteria appeared in 56.52% of GS and 52.17% of BC. MBRO (multidrug-resistant organisms) bacteria were identified in the proportion of 11%, then 13% from ESBL (extended-spectrum beta-lactamase) bacteria, and they remain as 4% MRSA (methicillin-resistant Staphylococcus aureus) bacteria. The higher result, 76% of the data was confirmed from the non-ICU patients.Conclusion: GS can be used as a reference for empiric antibiotic therapy due to its effectiveness, and it has a high degree of similarity with positive blood culture results.  Latar belakang: Salah satu pertimbangan pemberian antibiotika empiris pada kasus bakteremia adalah berdasarkan hasil pewarnaan gram. Dibutuhkan hasil yang akurat dan cepat dalam membedakan bakteri penyebab infeksi. Namun sedikit data tentang berapa besar kesesuaian hasil pewarnaan gram dengan pertumbuhan bakteri pada kultur darah. Untuk membandingkan hasil pewarnaan gram dengan pertumbuhan bakteri pada kultur darah yang positif.Metode: Penelitian ini merupakan penelitian analitik cross-sectional. Data diperoleh dari hasil VITEK® 2 Compact (bioMérieux) selama enam bulan (Januari – Juni 2020). Sampel penelitian adalah semua kultur darah yang diperiksa pada periode penelitian berjumlah 509.Hasil: Dari 509 sampel spesimen darah, 46 sampel dilaporkan sebagai nilai kritis prediktor bakteremia. Bakteri batang gram negatif teridentifikasi pada 39,13% sampel pewarnaan gram dan 45,65% sampel hasil kultur darah. Bakteri gram positif muncul pada 56,52% sampel pewarnaan gram dan 52,17% sampel hasil kultur darah. Bakteri MDRO (multidrug resistant organisms) teridentifikasi sebanyak 11%, 13% bakteri ESBL (extended spectrum beta lactamase), dan 4% bakteri MRSA (methicillin-resistant Staphylococcus aureus). Proporsi 76% sampel berasal dari pasien non-ICU.Simpulan: Hasil pewarnaan gram dapat digunakan sebagai acuan terapi antibiotika empiris karena memiliki tingkat kesesuaian yang tinggi dengan hasil kultur darah positif.
Co-Authors Agus Eka Darwinata Anak Agung Gde Putra Wiraguna Arisandy Achmad Bagus Ngurah Putu Arhana Cynthia Sofyanti Sugiharto Dady Iskandar Delly Dahlia Desak Made Wihandani Gede Bagus Gita Pranata Gede Bagus Gita Pranata, Gede Bagus Gita Gusti Ayu Dianti Violentina Hayyu Kusumaningtyas I Dewa Made Sukrama I Dewa Putu Sutjana I Gusti Made Aman I Ketut Badjra Nadha I Ketut Suada I Komang Kari I Made Gustama Heryawan I Made Jawi I Made Reza Pramudya I Putu Adiartha Griadhi I Putu Gede Septiawan Saputra I Wayan Juli Sumadi Ida Ayu Santhi Pertiwi Manuaba Ida Bagus Subanada Indramawan Setyojatmiko J. A. Pangkahila Ketut Erna Bagiari, Ketut Erna Ketut Rina, Ketut Komang Januartha Putra Pinatih Kusumajaya, I Gusti Bagus Ari Adi Luh Putu Ratna S Made Agus Hendrayana Muhammad Ali Imron Ni Made Adi Tarini Ni Nengah Dwi Fatmawati Ni Nyoman Sri Budayanti Ni Putu Siadi Purniti Ni Wayan Winarti Noviana Indrawati Olivia Julita Paramyta, I Gusti Ayu Cintya Putu Yoska Arya Harindana Saraswati, Ni Ketut Rake Putri Shalihaty Emy Sugijanto - Susy Purnawati Wimpie I Pangkahila Yuliana Tjua