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PENGARUH LAMA APLIKASI DAN WAKTU PERENDAMAN GIGI DENGAN JUS BUAH PIR TERHADAP PERUBAHAN WARNA DAN KEKERASAN MIKRO Dewi Ratma Utami; Andina Rizkia Putri Kusuma; Welly Anggarani
ODONTO : Dental Journal Vol 3, No 2 (2016): December 2016
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.3.2.111-117

Background: Teeth discoloration is considered disturbing performance,therefore bleaching become an option for teeth whitening. Hydrogen Peroxide (H2O2) is commonly used, it is beneficial for teeth whitening but in other hand it has adverse effectin lowering micro hardness of the tooth. Hydrogen Peroxide can be found in pears. The objectives of this research were to determine the influences of application duration and immersion time of tooth when immersed in pear juice toward its discoloration and micro hardness.Method: An experimental study with pre-post test controlled group design were conducted using 28 premolars teeth which divided into four groups. Group A and Bwere immersed in pear juice with concentration of 100% for 30 and 60 minutes/day. Group C and D were immersed in H2O2 3% for 30 and 60 minutes/day each for 14 days. Tooth color and micro hardness of each tooth sample were recorded before and on 7thand 14thdays after immersion. The data was analyzed by friedman test, repeated anova, one-way anova and post hoc.Result: The average of tooth color index based on the duration of application in all four groups showed significant changes, while the average change in micro hardness index based on the duration of applications were shown significant difference only in group C and D. The difference of time immersion in pear juice 100% for 30 and 60 minutes had no effect on tooth discoloration and micro hardness.Conclusion: It can be concluded that pear juice can be used as an alternative material for bleaching process without deteriorating it’s micro hardness.

PERBEDAAN EFEKTIVITAS CHLORHEXIDINE GLUKONAT 0,2% DENGAN TEH HIJAU (CAMELLIA SINENSIS) TERHADAP JUMLAH PORPHYROMONAS GINGIVALIS Devi Dwipriastuti; R. Rama Putranto; Welly Anggarani
ODONTO : Dental Journal Vol 4, No 1 (2017): July 2017
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.4.1.50-54

Background: Periodontitis is a periodontal inﬂammation caused by plaque that contains pathogens, Porphyromonas gingivalis is one of them. Chlorhexidine therapy used to reduce the number of pathogenic bacteria causing periodontitis. Green tea contains polyphenols such as epigallocatechin-3-gallic as antibacterial agent that can kill the bacteria Porphyromonas gingivalis. This study aims to determine diﬀerences in the eﬀectiveness of chlorhexidine gluconate 0.2% with green tea (Camellia sinensis) various concentrations to decrease the amount of bacteria Porphyromonas gingivalis.Method: This research was experimental with post test only design. Method used in antibacterial test was a drop plate misra. The experimental group consisted of six groups: chlorhexidine gluconate 0.2% and green tea group with a concentration of 100%, 75%, 50%, 25% and 12.5%. minimum bactericidal concentrations againts Porphyromonas gingivalis was evidenced by counting the number of colonies that formed on agar. Analysis data was using One Way ANOVA continued by Post hoc tests Tamhane.Result: The results showed that chlorhexidine gluconate 0.2% with green tea eﬀective to decrease the amount of bacteria Porphyromonas gingivalis(ANOVA p <0.05). The results between the groups showed green tea 100%, 75% and 25%, have same eﬀect compared to chlorhexidine gluconate 0.2% in reducing Porphyromonas gingivalis.Conclusion: In this experiment showed that chlorhexidine gluconate 0.2% and green tea extract 100%, 75%, 50%, 25% and 12.5% were able to decrease the amount of Porphyromonas gingivalis.

THE EFFECT OF PREGNANCY MILK ON THE EXPRESSION OF KALLIKREIN RELATED PEPTIDASE-4 (KLK-4) AND COLLAGEN TYPE 1 (Coll-1) IN AMELOGENESIS Sandy Christiono; Welly Anggarani
Dentino : Jurnal Kedokteran Gigi Vol 6, No 2 (2021)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v6i2.11993

Background: Tooth development during embryonic period is a complex process and requires adequate nutrients for the formation of healthy dental tissues. Kallikrein-related peptidase-4 (KLK-4) and collagen type 1 (Coll-1) are serine proteinases secreted by ameloblast during the transition and maturation stages of the amelogenesis processes, functioning to degrade the protein matrixes, so that the enamel can reach its final hardness. Pregnancy milk contains various nutrients expected to increase the KLK-4 expression of ameloblast cells in tooth development processes Purpose: This study aimed at determining the influence of pregnancy milk on the KLK-4 and collagen type 1 (Coll-1) expression of ameloblast cells in the tooth development processes.study Method The research subjects comprised of 10 pregnant female mice (Mus Musculus L.) that were divided into: control group (given sterile aquadest) and treatment group (given pregnancy milk + sterile aquadest) for 18 days followed by the collection of the tooth germ. The specimens were then stained using Imunnohistochemistry to see the KLK-4 and Coll-1 expressions. The data were analyzed using a pathway analysis. Result: The average KLK-4 and Coll-1 expression in the treatment group were higher than those in the control group. Based the pathway analysis, there were direct correlation of Pregnancy milk with Coll-1 expression and that with KLK-4 and Coll-1 expression as well as indirect correlation of pregnancy milk with KLK-4 expression. Conclusion: Pregnancy milk influences the Kallikrein-related peptidase-4 (KLK-4) and Coll-1 expression of ameloblast cells in the tooth development of the mice’s fetusesKeywords: Coll-1 pregnancy milk, Kallikrein-related peptidase-4 (KLK-4), Tooth development

THE EFFECT OF SALTWATER FISH NANOPARTICLE POWDER CONSUMPTION ON TOOTH ENAMEL DENSITY In Vivo Study of Mice (Mus musculus) Sandy Christiono; Fera Putri Ardiani; Welly Anggarani; Fourier Dzar Eljabbar
Dentino : Jurnal Kedokteran Gigi Vol 6, No 1 (2021)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v6i1.10724

ABSTRACTBackground: Dental caries is rarely found in children who eat saltwater fish. Overfished fish can be used as powder of saltwater fish nanoparticles, which is useful as an alternative material for the prevention of dental caries in dentistry can be used as a powder for saltwater fish nanoparticles, which is useful as an alternative material for preventing dental caries in dentistry. A Cone Beam Computed Tomography (CBCT), commonly known as a μ-CT Scanner, is a device used to quantify the increase in tooth enamel density Purpose: This study aimed to determine the effectiveness of saltwater fish nanoparticle powder consumption on tooth enamel density of Mus musculus. Method This study used an experimental research method with a randomized posttest only control group design. The research subjects were 16 mice taken from 2 pregnant female mice which were divided into two groups, namely the treatment group with 2.17 mg / 0.5 mL of saltwater fish nanoparticle powder and the control group which was given distilled water. Administration was carried out during the intrauterine period until the teeth of the mice grew, then observed the tooth enamel density with CBCT OP 3D Pro (KaVo, Germany). The data obtained were analyzed by Levene and continued with the Independent T-test. Result: The average value of enamel density in mandibular incisors in the treatment group was greater than the control group (p <0.05). Conclusion: Saltwater fish nanoparticle powder can increase tooth enamel density of Mus musculus. Keywords: CBCT, CT nanoparticle powder, saltwater fish, tooth enamel density,

THE IMPACT OF CONSUMING SALMON FISH (SALMO SALAR) ON DENTAL CALCIUM LEVELS IN MICE (MUS MUSCULUS) Sandy Christiono; Naila Salsabila; Rizki Amalina; Welly Anggarani; Islamy Rahma Hutami
Dentino : Jurnal Kedokteran Gigi Vol 8, No 1 (2023)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v8i1.16086

ABSTRACTBackground: Pregnant women require calcium as a vital vitamin. Insufficient calcium intake during pregnancy can result in enamel hypoplasia and cavities in deciduous teeth. It can boost pregnant women's calcium intake with calcium-containing pill formulations and vitamin D; however, this has negative effects such as kidney stone issues. Salmon can be used as a substitute because it has no negative side effects and contains omega 3, which can enhance calcium absorption. Purpose: This study aimed to determine the effect of salmon (Salmo salar) consumption on calcium levels in the teeth of mice (Mus musculus). Method: This study used pregnant mice and a post-test-only control strategy. Pregnant mice were fed salmon combined with CMC (Carboxy Methyl Cellulose) and CMC (Carboxy Methyl Cellulose) alone as a control. The salmon dose administered is 2.17 mg/0.5 ml. Using atomic absorption spectrophotometry, mice dental calcium levels can be determined (AAS). Result: The findings of hypothesis testing with an unpaired t-test of 0.041 revealed a significant difference between the salmon-eating and CMC-consuming groups at p 0.05. (Carboxy Methyl Cellulose). Conclusion: Consuming salmon can significantly increase the calcium content of mouse teeth. Keywords: Dental calcium, Mice (Mus Musculus, Pregnant mice, Salmon (salmo nalar)

PENGARUH LAMA APLIKASI DAN WAKTU PERENDAMAN GIGI DENGAN JUS BUAH PIR TERHADAP PERUBAHAN WARNA DAN KEKERASAN MIKRO Dewi Ratma Utami; Andina Rizkia Putri Kusuma; Welly Anggarani
Odonto : Dental Journal Vol 3, No 2 (2016): December 2016
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Teeth discoloration is considered disturbing performance,therefore bleaching become an option for teeth whitening. Hydrogen Peroxide (H2O2) is commonly used, it is beneficial for teeth whitening but in other hand it has adverse effectin lowering micro hardness of the tooth. Hydrogen Peroxide can be found in pears. The objectives of this research were to determine the influences of application duration and immersion time of tooth when immersed in pear juice toward its discoloration and micro hardness.Method: An experimental study with pre-post test controlled group design were conducted using 28 premolars teeth which divided into four groups. Group A and Bwere immersed in pear juice with concentration of 100% for 30 and 60 minutes/day. Group C and D were immersed in H2O2 3% for 30 and 60 minutes/day each for 14 days. Tooth color and micro hardness of each tooth sample were recorded before and on 7thand 14thdays after immersion. The data was analyzed by friedman test, repeated anova, one-way anova and post hoc.Result: The average of tooth color index based on the duration of application in all four groups showed significant changes, while the average change in micro hardness index based on the duration of applications were shown significant difference only in group C and D. The difference of time immersion in pear juice 100% for 30 and 60 minutes had no effect on tooth discoloration and micro hardness.Conclusion: It can be concluded that pear juice can be used as an alternative material for bleaching process without deteriorating it’s micro hardness.

PERBEDAAN EFEKTIVITAS CHLORHEXIDINE GLUKONAT 0,2% DENGAN TEH HIJAU (CAMELLIA SINENSIS) TERHADAP JUMLAH PORPHYROMONAS GINGIVALIS Devi Dwipriastuti; R. Rama Putranto; Welly Anggarani
Odonto : Dental Journal Vol 4, No 1 (2017): July 2017
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Periodontitis is a periodontal inﬂammation caused by plaque that contains pathogens, Porphyromonas gingivalis is one of them. Chlorhexidine therapy used to reduce the number of pathogenic bacteria causing periodontitis. Green tea contains polyphenols such as epigallocatechin-3-gallic as antibacterial agent that can kill the bacteria Porphyromonas gingivalis. This study aims to determine diﬀerences in the eﬀectiveness of chlorhexidine gluconate 0.2% with green tea (Camellia sinensis) various concentrations to decrease the amount of bacteria Porphyromonas gingivalis.Method: This research was experimental with post test only design. Method used in antibacterial test was a drop plate misra. The experimental group consisted of six groups: chlorhexidine gluconate 0.2% and green tea group with a concentration of 100%, 75%, 50%, 25% and 12.5%. minimum bactericidal concentrations againts Porphyromonas gingivalis was evidenced by counting the number of colonies that formed on agar. Analysis data was using One Way ANOVA continued by Post hoc tests Tamhane.Result: The results showed that chlorhexidine gluconate 0.2% with green tea eﬀective to decrease the amount of bacteria Porphyromonas gingivalis(ANOVA p <0.05). The results between the groups showed green tea 100%, 75% and 25%, have same eﬀect compared to chlorhexidine gluconate 0.2% in reducing Porphyromonas gingivalis.Conclusion: In this experiment showed that chlorhexidine gluconate 0.2% and green tea extract 100%, 75%, 50%, 25% and 12.5% were able to decrease the amount of Porphyromonas gingivalis.

The Effect of Adding Propolis to Formula Milk on The Streptococcus Sobrinus Inhibition Zone (In Vitro) Welly Anggarani; Adisty Restu Poetri; Dinda Nabila Alfani
Denta Journal Kedokteran Gigi Vol 18 No 1 (2024): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v18i1.5

Background: Caries is one of the dental and oral health problems that are often suffered by children. One of the main causes is the habit of children consuming formula milk as it contains sucrose. Streptococcus sobrinus is an oral pathogenic bacteria that has a role in the initial process of dental caries. One of the natural ingredients that function as anti-caries is propolis. Objective: Therefore, this study aims to determine the effect of adding propolis to formula milk on the inhibition zone of Streptococcus sobrinus bacteria. Methods: This type of research is a Laboratory Experiment with a Post-Test control Group Design. It consists of 4 groups, namely the formula milk group without propolis as a negative control, the formula milk treatment group with propolis concentrations of 5%, 10%, and 20% carried out with 7 replications of each group. The sample is a pure isolate of Streptococcus sobrinus bacteria. Results: The results showed the average inhibition zone was the formula milk group without propolis was 0.00±0.00 mm, the formula milk group with propolis concentration of 5% was 12.87±1.18 mm, the formula milk group with propolis concentration of 10% was 16.65±1.51 mm, and the formula milk group with propolis concentration of 20% was 24.87±0.39 mm. Conclusion: It means the addition of propolis to formula milk has been proven to be effective in inhibiting the growth of Streptococcus sobrinus bacteria.

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