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All Journal Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Bioma : Berkala Ilmiah Biologi JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA Biosaintifika: Journal of Biology & Biology Education Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Spizaetus: Jurnal Biologi dan Pendidikan Biologi Biosaintifika: Journal of Biology & Biology Education

Sri Isdadiyanto

Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali

Author-ID : 288951

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Public Health Veterinary Other

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Endotelium Arteria Coronaria Tikus Putih Sprague Dawley Hiperlipidemia setelah diberi Chitosan Cangkang Udang Laut (Penaeus monodon F.) Isdadiyanto, Sri
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 24, No 1 (2016): Volume 24, nomor 1, tahun 2016
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

The objective of this study was to analyze the influence of chitosan on arteria coronaria endothelium of Sprague Dawley rats induced by high fat ration. The animals for this study were twenty adult male rats divided into four groups, i.e. group I as the control was fed with basal ration containing normal fat for 3 months, group II was fed ration containing high fat for 3 months, group III was fed ration containing high fat and given chitosan 180 mg per kg body weight per day orally in 2 ml aquadest for 3 months, group IV was fed ration containing high fat for 3 months and after 1 month given chitosan 180 mg per kg body weight per day orally in 2 ml aquadest for 2 months. Each group consisted of five animals. After 90 days, the rats were necropsied and the heart were collected to histopathological. CRP expression on coronary artery of rats that given normal diet were negative, whereas rats that given high fat diet and no treatment were positive. CRP expression of coronary artery for rats that given high fat diet and given chitosan were negative, but rats that given chitosan after one month were positive. Based on the result of this study, it was concluded that high fat ration was a major factor induced inflammation in coronary artery and chitosan was able to prevent disfunction of arteria coronaria endothelium cel. Â Keywords: Atherosclerosis, arteria coronaria endothelium, chitosan, high fat ration.

KADAR APOPROTEIN A DAN APOPROTEIN B SERUM DARAH TIKUS PUTIH Sprague Dawley HIPERLIPIDEMIA SETELAH DIBERI CANGKANG UDANG LAUT (Penaeus monodon F.) Isdadiyanto, Sri
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 23, No 2 (2015): Volume XXIII, Nomor 2, Oktober 2015
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Â The objective of this study was to analyze the Â apoproteinÂ AÂ and apoprotein B level of Sprague Dawley rats induced by high fat ration after given of sea shrimp carapace (Penaeus monodon F.). The animals for this study were twenty adult male rats divided into four groups, i.e. group I as the control was fed with basal ration containing normal fat for 3 months, group II was fed ration containing high fat for 3 months, group III was fed ration containing high fat and given sea shrimp carapace 180 mg per kg body weight per day orally in 2 ml aquadest for 3 months, group IV was fed ration containing high fat for 3 months and after 1 month given sea shrimp carapace 180 mg per kg body weight per day orally in 2 ml aquadest for 2 months. Each group consisted of five animals. After 90 days, the rats were necropsied and the blood were collected to analyzes Â of apoprotein A and apoprotein B level. Apoprotein A level and Apoprotein B level were measured using colorimetric enzymatic method and Roche/Hitachi cobas c systems automatically calculate. Â Â The result showed that high fat diet increased Â of apoprotein B level, sea shrimp carapace increased Â of apoprotein A level and decreased Â of apoprotein B level. Â Keywords : Sea shrimp carapace, Sprague Dawley rats, apoprotein A and apoprotein B levelÂ

PENGARUH PEMBERIAN MIKROMINERAL (Fe, Co, Cu, Zn), VITAMIN (A, B1, B12, C) DAN JUS MENGKUDU (Morinda citrifolia L.) TERHADAP KONSUMSI PAKAN, BOBOT LEMAK ABDOMINAL DAN JUMLAH FOLIKEL OVARIUM YANG BERKEMBANG PADA PUYUH (Coturnix coturnix japonica L.) Kurnia, Shinta Dwi; Saraswati, Tyas Rini; Isdadiyanto, Sri
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 23, No 2 (2015): Volume XXIII, Nomor 2, Oktober 2015
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

The purpose of the research was evaluated feed consumption, abdominal fat and amount mature folliclesfrom cultivated with combination solution of microelements (Fe, Co, Cu, Zn), vitamins (A, B1, B12, C) and morinda juice (Morinda citrifolia L.) as drinking water to optimization the productincultivation of the quail.Â Sixty quails (Coturnix coturnix japonica L.) fourteen days were divided into four treatments of combination solution microelements, vitamins and morinda juice with 15 quails in each treatments. The treatments were control, microelements and vitamins, morinda juice, and minroelements, vitamins and morinda juice. Experimental data were analyzed by using ANOVA based on completely randomized design. The result showed that combination solution of microelements, vitamins and morinda juice significant in feed consumption. Morinda juice increased feed consumption so that solution must not be used in cultivation technique of quail. Microelements and vitamins combination decreased feed consumption but not influential to mature follicles. Keywords: microelements, vitamins, morinda juice, Coturnix coturnix japonica L.

EFEK CHITOSAN PADA HISTOPATOLOGIS AORTA TIKUS PUTIH YANG DIBERI PAKAN LEMAK TINGGI Isdadiyanto, Sri
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 23, No 1 (2015): VOLUME XXIII, NOMOR 1, MARET 2015
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

The objective of this study was to analyze the influence of chitosan on aorta histopatologic of Sprague Dawley rats induced by high fat ration. The animals for this study were twenty adult male rats divided into four groups, i.e. group I as the control was fed with basal ration containing normal fat for 3 months, group II was fed ration containing high fat for 3 months, group III was fed ration containing high fat and given chitosan 180 mg per kg body weight per day orally in 2 ml aquadest for 3 months, group IV was fed ration containing high fat for 3 months and after 1 month given chitosan 180 mg per kg body weight per day orally in 2 ml aquadest for 2 months. Each group consisted of five animals. After 90 days, the rats were necropsied and the aortas of heart were collected to histopathological. Histopathologic analysis of aortas using hematoxylin-eosin staining method and were analysis by descriptive. The rats given normal diet did not induce atheroma plaque. The rats given high fat diet induced atheroma plaque. The rats given high fat and given chitosan simultaneously did not induce atheroma plaque. The rats given high fat and after 1 month given chitosan 40% did not induce atheroma plaque and 60% induced atheroma plaque. Based on the result of this study, it was concluded that high fat ration was a major factor able to cause atherosclerosis and chitosan was able to prevent atheroma plaque formation. Â Key words: Atherosclerosis, aorta, atheroma plaque, chitosan, high fat ration.

Kadar Kolesterol Kuning Telur dan Daging Puyuh Jepang (Coturnix-coturnix japonica L.) setelah Pemberian Suplemen Serbuk Kunyit (Curcuma longa L.) Putra, Sukarman Hadi Jaya; Saraswati, Tyas Rini; Isdadiyanto, Sri
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 24, No 1 (2016): Volume 24, nomor 1, tahun 2016
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

This study aims to determine the effect of supplementation powder turmeric ( Curcuma longa ) before sexuality aging periods on cholesterol levels of eggs and meat Japanese quail ( Coturnix japonica coturnic ). Completely Randomized Design was applied in this research. Test animals used were 45 female Japanese quails were divided into three groups, P0 : Japanese quail are not given turmeric powder , P1 : Japanese quail given turmeric powder at a dose of 54 mg / individu / day , P2 : Japanese quail given turmeric powder at a dose of 108 mg / individu / day . The treatmant was repeated in 15 times. The treatment was given every day on 14th-44th day quail. The eggs were collected and analyzed the levels of cholesterol in the yolk , whereas cholesterol levels of meat taken after the Japanese quail was depicated . Data were analyzed using analysis of variance ( ANOVA ) followed by Duncan test with 95% significantly level ( Î± = 0.05). Analysis software is used Minitab software 16. Results showed that supplementationÂ of turmeric powder before sexuality aging periods on cholesterol levels Japanese quail eggs and meat a significant effect ( PË‚0.05 ) lower than the control. The results showed that doses up to 108 mg of turmeric powder / head / day can lower cholesterol Japanese quail eggs and meat . Â Keywords : Curcuma longa, Coturnix coturnix japonica, meat cholesterol, egg cholesterol

Indeks Kuning Telur dan Nilai Haugh Unit Telur Puyuh (Coturnix coturnix japonica L.) Hasil Pemeliharaan dengan Penambahan Cahaya Monokromatik Lestari, Wulan Tri; Tana, Silvana; Isdadiyanto, sri
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 24, No 1 (2016): Volume 24, nomor 1, tahun 2016
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

The aims of this study was to analyze the quality of quail eggs after treatment with the addition of monochromatic red, green and blue light reviewed from the yolk index and Haugh unit values. Treatment started from 4 to 12 weeks. This study used 5 Watts voltage as light resources in the night Â during 12 hours / day. Parameters in this study were index ofÂ egg yolk (IKT), the Haugh Unit value (HU), feed intake, drink intake, and the weight of the egg. The data of this study was analyzed by Analysis of variance (ANOVA), and if significantly differences continued with Duncan test at 5% significance level. The data researched with blue light treatment showed the IKT and HU was highest value, but the lowest showed on the red light. The highest feed intake on control, but the drink intake didnâ€™t show significant differences. The highest weight of egg showed on the red light treatment. The conclusions of this study was the addition of monochromatic blue light was increased IKT and HU value, therefor had potent to enhance the quality of eggs. Keywords: quail (Coturnix coturnix japonica L.), monochromatic light, egg productivity, IKT, HU

PENGARUH PEMBERIAN LARUTAN KOMBINASI MIKROMINERAL (Cu, Fe, Zn, Co), VITAMIN (A, B1, B12, C) DAN JUS BUAH MENGKUDU (Morinda citrifolia L.) SEBAGAI AIR MINUM TERHADAP KONSUMSI MINUM DAN BOBOT KARKAS PUYUH (Coturnix coturnix japonica L.). Arumsari, Dina; Saraswati, Tyas Rini; Isdadiyanto, Sri
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 23, No 2 (2015): Volume XXIII, Nomor 2, Oktober 2015
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Quail (Coturnix-coturnix japonica L.) was becoming more popular in Indonesia because of the product that produce was substantial enough like the meat and egg. This animal often used as an experiment in order to enhance itâ€™s productivity. This research was to find out the potential of giving combination of micromineral (Cu, Fe, Zn, Co), vitamin (A, B1, B12, C) and morinda juice (Morinda citrifolia L.) solution as drinking water to drink consumption and weight of carcass. The treatment was begun when the quail was in age of 4 to 16 weeks. The experiment in this research was nonfactorial experimentation with complete random plan (RAL). The obtained data was analyzed by using the analysis of varian (ANOVA) and if there was a real difference then it will be done an advanced testing by using the Duncan test in confidence level 95%. According to the analysis result towards the drink consumption and the weight of carcass indicate any real difference inter cluster of quail whose itâ€™s drink was added by combination of micromineral (Cu, Fe, Zn, Co), vitamin (A, B1, B12, C) and morinda juice with control, in conclusion that giving combination of micromineral (Cu, Fe, Zn, Co), vitamin (A, B1, B12, C) and morinda juice has the potency in the drinking management. Keywords : Coturnix coturnix japonica L., micromineral, vitamin, morinda juice

MIKROANATOMI HEPAR MENCIT (Mus musculus) SETELAH PEMBERIAN KITIN PER-ORAL Isdadiyanto, Sri
JURNAL SAINS DAN MATEMATIKA Volume 17 Issue 2 Year 2009
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2956.474 KB)

ABSTRACT--TheÂ objective ofÂ the experimentÂ was determinesÂ liverÂ microanatomyÂ and diet consumptionÂ nÂ the Mus muscalusÂ after give ofÂ chitin. This researchÂ usedÂ completelyÂ randomizedÂ design. ThereÂ wereÂ fowÂ concentrationÂ of chitin, 0 mg chitin per day; 1,3 mg chitin per day; 1,95Â mg chitin per day; 2,6 mg chitin per day. AnovaÂ wasÂ usedÂ in dataÂ analysisÂ andÂ LSDT testÂ in 57oÂ level. TheÂ resultÂ of experiment indicatedÂ thatÂ theÂ chitin wasÂ not cause alterationÂ onÂ liver microanatomy.Keywords:Â chittin, Mus musculus, liver microanatomy

STRUKTUR HEPAR DAN RASIO BOBOT HEPAR TERHADAP BOBOT TUBUH MENCIT (MUS MUSCULUS L.) JANTAN SETELAH PEMBERIAN EKSTRAK ETANOL DAUN MIMBA (AZADIRACHTA INDICA A.JUSS) Fitriani, Ratih Nur; Sitasiwi, Agung Janika; Isdadiyanto, Sri
Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Volume 5, Nomor 1, Tahun 2020
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.5.1.2020.%p

Penelitian ini bertujuan untuk mengetahui struktur hepar dan rasio bobot hepar terhadap bobot tubuh mencit (Mus musculus L) jantan setelah pemberian ekstrak etanol daun mimba (Azadirachta indica A.Juss). Penelitian ini menggunakan 2 kelompok perlakuan yaitu Kontrol (akuades) dan Perlakuan (mimba). Perlakuan diberikan secara oral selama 21 hari. Hepar ditimbang dan dibuat preparat menggunakan metode parafin dengan pewarnaan Hematoksilin Eosin. Pengukuran diameter hepatosit dilakukan dalam 4 bidang pandang. Bobot hepar dan bobot tubuh digunakan untuk menghitung nilai HSI. Data dianalisis dengan uji t. Hasil analisis menunjukan adanya pengaruh nyata terhadap diameter hepatosit tetapi menunjukan pengaruh tidak nyata terhadap bobot tubuh, bobot hepar dan nilai Hepatosomatik indek (HSI). Hal ini dapat disimpulkan bahwa pemberian ekstrak etanol daun mimba (Azadirachta indica) sebagai obat tradisional dengan dosis 14 mg/KgBB tidak mempengaruhi struktur hepar dan rasio bobot hepar terhadap bobot tubuh mencit (Mus musculus) jantan. Kata kunci : Daun Mimba, Hepar, Diameter Hepatosit dan HSI.

JUMLAH SEL LEYDIG DAN STRUKTUR MIKROANATOMI TESTIS TIKUS PUTIH (RATTUS NORVEGICUS) SETELAH PEMBERIAN TEH KOMBUCHA KONSENTRASI 50% WAKTU FERMENTASI 6, 9 DAN 12 HARI Isdadiyanto, Sri; Tana, Silvana
Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Volume 5, Nomor 1, Tahun 2020
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.5.1.2020.%p

Tujuan penelitian ini untuk mengkaji potensi teh Kombucha kadar 50% dengan variasi waktu fermentasi dalam mempengaruhi jumlah sel leydig dan struktur mikroanatomi testis tikus putih (Rattus norvegicus). Penelitian ini menggunakan Tikus putih jantan sebanyak 16 ekor umur 2 bulan, dengan perlakuan Teh kombucha yang difermentasi selama 6, 9 dan 12 hari pada suhu 25oC per oral. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 4 perlakuan (selama 28 hari) dan 4 ulangan, yaitu : P0 = kontrol, tanpa tambahan teh kombucha, P1 = air minum + 1,8 ml teh kombucha pagi dan sore fermentasi 6 hari, P2 = air minum + 1,8 ml teh kombucha pagi dan sore fermentasi 9 hari, P3 = air minum + 1,8 ml teh kombucha pagi dan sore fermentasi 12 hari. Variabel yang diukur adalah diameter tubulus seminiferus dan jumlah sel Leydig. Data yang diperoleh dianalisis menggunakan ANOVA yang dilanjutkan dengan Uji Duncan dengan taraf kepercayaan 95% dengan menggunakan perangkat lunak SPSS 16.0. Penelitian ini menunjukkan bahwa pemberian teh kombucha fermentasi 6, 9, dan 12 hari menurunkan diameter tubulus seminiferus sehingga berpotensi mengganggu proses spermatogenesis, tetapi tidak mempengaruhi jumlah sel Leydig tikus putih (Rattus norvegicus). Kata kunci: Rattus norvegicus, teh kombucha , tubulus seminiferus, sel Leydig

Co-Authors Aetin, Entin Nur Agung Janika Sitasiwi Agus Subagio Alfatinnisa, Zalfa Andriyan, Mohammad Wahyu Baiq Miftahul Jannah Budiwardani, Dwinanda Chatarina Umbul Wahyuni Dina Arumsari, Dina Djaelani, M Anwar Djati, Astrid Laksmita Endah Puji Astuti, Endah Puji Enny Yusuf Wachidah Yuniwarti Enny Yusuf Wachidah Yuniwarti Ermanjaya, Laode Fajar, Stefanus Fathoni, Nasrul Febriani, Lili Fitriani, Ratih Nur Jaya, La Ode Irman Kasiyati Kasiati Khairunnisa, Najla Athallah Kustopo Budiraharjo Nuke Nur Hidayati, Nuke Nur Nursabrina, Azra Batrisyia Rohman, Jihadul Hanif Fadlur Saputra, Muhammad Ragil Savitri, Ajeng Fatria Shinta Dwi Kurnia Sijabat, Agave Chintya Gabriella Silvana Tana Siti Muflichatun Mardiati Sunarno , Sunarno Sunarno Sunarno Syakera, Cinta Etna Tyas Rini Saraswati Wulan Tri Lestari, Wulan Tri

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