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Iswendi iswendi
Dosen Kesehatan Masyarakat, Fakultas Kedokteran Universitas Andalas, Padang
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Physics Social Sciences Other

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Journal : Sainstek : Jurnal Sains dan Teknologi

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PENENTUAN AKTIVITAS AMILASE DARI UMBI BENGKUANG (Pachyrrizus arosus L.Urb) HASIL EKSTRAKSI DENGAN ETANOL DAN AMMONIUM SULFAT Iswendi, Iswendi
Sainstek : Jurnal Sains dan Teknologi Vol 2, No 2 (2010)
Publisher : IAIN Batusangkar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (520.783 KB) | DOI: 10.31958/js.v2i2.13

Abstract

This research aimed to determine the optimum amylase activity at the variations of temperature and pH, and the effectiveness of extractors.  The extraction of the enzyme can be done in two ways, with an organic solvent and inorganic salt. We have conducted research on amylase activity of yam tubers extracted with ethanol and ammonium sulfate. This research is an experimental research using complete randomized design with two factorials which are pH and temperature. pH variations were 4, 4.5, 5.0, 5.5, and 6, while the variation of temperature are 300 C, 400 C, 500C, and 600C. Isolation of amylase-was carried out by multilevel fractionation using saturated ammonium sulphate 40, 60. 80, and 90% w/v and cold ethanol 95%. Amylase activity was determined based on the concentration of glucose which is result of hydrolysis of starch by Nelson-Somogy method. The highest activity was found in fraction I, pH 4 and temperature of 500C with 34.55 units of activity for ethanol solvent and 30.72 units for ammonium sulfate. Key words: amylase, activity, isolation, yam tubers extracted
Co-Authors Amanda, Additya Ana, Aziza Putri Azzara, Salsabilla ‘Aini, Faizah Qurratul Desy Kurniawati FARISI, AHMAD IHSAN Febriani, Yona Fitri Amelia Fitriza, Zonalia Hidayat, Basultan Hikmah, Chofizah Nurul Husni, Hikmah Ibnu Iryani Iryani Lantusia, Marina Lovia, Mutiara Maharani, Denisa Ningsih, Puji Aptriyani Anggelia Putri, Salsabila Hasna Putri, Widya Rahayu Rahmadani, Ananda Ramadhani, Nurul Fitri Rasyid, Irfan Aminullah Rinata, Taufik Maulana Sari, Indah Fedya vauzia, wiwin Wini Rahma Yuda Yuliana, Yusi