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All Journal JPMS (Jurnal Pendidikan Matematika dan Sains) MAJALAH ILMIAH PEMBELAJARAN Jurnal Penelitian Saintek INOTEKS : Jurnal Inovasi Ilmu Pengetahuan, Teknologi, dan Seni EKSAKTA: Journal of Sciences and Data Analysis Jurnal Pendidikan Kimia Indonesia Jurnal Sains Dasar Jurnal Matematika & Sains Indonesian Journal of Biotechnology Indonesian Journal of Science and Technology Jurnal Penelitian Pendidikan IPA (JPPIPA) Indonesian Journal of Chemistry Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Molekul: Jurnal Ilmiah Kimia Musamus Journal of Primary Education Chemistry Education Practice Jurnal Inovasi Pembelajaran Kimia (Journal Of Innovation in Chemistry Education) Indonesian Journal Of Educational Research and Review Molekul: Jurnal Ilmiah Kimia Jurnal Riset Pembelajaran Kimia Jurnal Pendidikan MIPA

Retno Arianingrum

Universitas Negeri Yogyakarta

Author-ID : 231928

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics Social Sciences Other

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Silikat dan Titanium Silikat Mesopori-Mesotruktur Berbasis Struktur Heksagonal dan Kubik Hari Sutrisno; Retno Arianingrum; Ariswan Ariswan
Jurnal Matematika & Sains Vol 10, No 2 (2005)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Silicalite (MCM-41 & MCM-48) and titanium silicalite (Ti-MCM-41 & Ti-MCM-48) mesoporous-mesostucture material have been synthesized and studied. Its have been obtained from hydrolysis and condensation of reactant : tetraethyl orthosilicate (TEOS) as silicium source and cetyltrimethyl ammonium bromide (CTAB) surfactant as template, NaOH as base and water solution. Ti-MCM-41 & Ti-MCM-48 mesoporous-mesostucture material have been synthesized from crystal of [Ti8O12(H2O)24]Cl8.HCl.7H2O as titanium source. Surfactant was removed by calcinations at 5500C for 5 hours. MCM-41 and Ti-MCM-41 are crystallized on the hexagonal structure, plane group p6 with unit cell parameter: a = 44.09 and 49.48 Çº respectively, meanwhile MCM-48 and Ti-MCM-48 are crystallized on the cubic structure, space group Ia3d with unit cell parameter: a = 87.46 and 94.42 Çº respectively.

IDENTIFICATION AND BIOLOGICAL ACTIVITY TEST SOME ISOLATED COMPOUNDS FROM STEM BARK OF MELINJO (GNETUM GNEMON) Atun, Sri; Arianingrum, Retno; Masatake, Niwa
Jurnal Sains Dasar Vol 1, No 1 (2012): April 2012
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Isolation and structure elucidation of two compounds, namely resveratrol (1), and 3- methoxyresveratrol (2) from stem bark of Melinjo (Gnetum gnemon) had been done. The isolation of those compounds was carried out by chromatographyc method and structure elucidation was performed by interpretation of spectroscopic data, including UV, IR, 1H and 13C NMR 1D and 2D, and FABMS. The result of this study showed that activity each compounds as radical hydroxyl scavenger of resveratrol (1), and 3-methoxyresveratrol (2), with an IC50 45,17 and 60,12; g/ml respectively. Each compound showed significant activity as UV-B protection. Activity test as UV-B protection showed that resveratrol and methoxyresveratrol have maximum protections (SPF 8,03 and 12,34 respectively), each compounds on 50 g/ml. Key word : melinjo; Gnetum gnemon; natural antioxidant; UV-B protection

PEMANFAATAN EKSTRAK DAUN JARAK PAGAR DAN LIMBAH MINYAK GORENG SEBAGAI SABUN ANTIBAKTERI Aris, Asmi; Gistiana, Ilyas; Hanafia, Hafiizhoh; Arianingrum,M.Si., Retno
Jurnal Penelitian Saintek Vol 26, No 2 (2021)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v26i2.40594

Penelitian ini bertujuan untuk mengetahui karakteriktik sabun dari limbah minyak goreng dengan bahan aditif ekstrak daun jarak pagar (Jatropha curcas Linn) danÂ menguji aktivitas bakterinya terhadap bakteri Staphylococus aureus dan Escherichia coli. Sabun dibuat melalui reaksi saponifikasi NaOH dengan limbah minyak goreng.antibakteri dengan bahan tambahan ekstrak daun jarak pagar (Jatropha curcas Linn) berdasarkan persyaratan SNI dan aktifitas antibakteri sabun dengan ekstrak daun jarak pagar terhadap bakteri Staphylococus aureus dan Escherichia coli. Metode yang digunakan dalam penelitian ini yaitu ekperimen laboratorium, dengan melakukan ekstraksi, pembuatan sabun, analisis karakteristik sabun dan uji aktifitas antibakteri. Hasil percobaan menunjukkan bahwa ektrak daun jarak pagar memiliki kandungan senyawa aktif fenol, saponin, tanin, dan flavonoid. Berdasarkan analisis ANOVA variasi konsentrasi ekstrak memberikan pengaruh sgnifikan pada pH dan kadar akali bebas sabun. Sabun menunjukan akivitas anti bakteri Staphylococus aureus dan Escherichia coli yag diketahui melalui pengukuran zona bening. Penelitian ini dapat diaplikasikan dimasyarakat sehinga memiliki prospek yang baik dari segi lingkungan dan ekonomi.

Synergistic effects of para-hydroxy meta-methoxy chalcone (pHmMC)- doxorubicin treatments on T47D breast cancer cells Retno Arianingrum; Retno Sunarminingsih; Edy Meiyanto; Sofia Mubarika
Indonesian Journal of Biotechnology Vol 20, No 2 (2015)
Publisher : Universitas Gadjah Mada

Resistance to some cancer chemotherapeutic drugs has been identifed. One strategy to overcome that problem is by combining two or more of the drugs to get co-chemotherapeutic effects. A derivate chalcone, 3 - (4’-hydroxy-3’-methoxyphenyl)-1-phenyl-2-propene-1-on or para hydroxy meta methoxy chalcone (pHmMC), has been reported to have cytotoxic activity on some cancer cells through some pathways. The aim of this study was to investigate the effects of combinations of pHmMC and Doxorubicin (Dox) on the cytotoxicity, anti-proliferation, apoptosis, and the cell cycle of T47D (breast cancer cell-lines) in vitro. The cytotoxic and antiproliferative activity were determined by MTT (3-[4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide] assay. The combination index (CI) was used to determine the synergistic, additive or antagonistic effects of the combinations. Flowcytometry method was performed to determine the combination effects on the apoptosis and cell cycle. The results indicated that the combinations had a higher inhibitory effect on the cell growth compared to those of single treatments of pHmMC and Dox. All the doses used in the combinations were lower of the single doses at their IC50s. The results showed all the combinations gave synergistic (CI: 0.3 – 0.7) up to strong synergistic (CI: 0.1 – 0.3) effects. The synergistic effects of the combinations were due to increased apoptosis and induced cell cycle arrest in S and G2/M phases on the cancer cell lines.

PENGARUH MEDIA TERHADAP EFEKTIVITAS PEMBELAJARAN PRAKTIKUM BIOKIMIA Eddy Sulistyowati; Retno Arianingrum; Togu Gultom; Das Salirawati
MAJALAH ILMIAH PEMBELAJARAN No 2 (2006): Jurnal Majalah Ilmiah Pembelajaran Edisi Oktober 2006
Publisher : MAJALAH ILMIAH PEMBELAJARAN

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.144 KB)

The objectivity of this research is to determine media that effective to studying in biochemistry practical work to improve student achievement.The sample of this research is all students of biochemistry practical work at odd semester in 2003/2004 in Chemistry Department in Faculty of Mathematics and Natural Science, at Yogyakarta State University. The student’s number was 28 people. The research was carried out using 3 media, i.e. Over Head Transparent presented by Over Head projector (OHP) at carbohydrate topic; Power Point presented by LCD projector at protein topic, and Audio-Visual presented by LCD projector at enzyme topic. The instruments of this research are observation sheet, pre-test and post-test, and evaluation questionnaire. The increase of post-test to pre-test was evaluated using t-test. The analyzed of observation data was carried out by qualitative descriptive using calculation percentage continued by qualitative analyzed. The mean of observation data was analyzed by ANAVA-A test followed by LSDT test. The data of evaluation questionnaire was calculated as percentage of student choice at this questionnaire.The result of observation data showed that there was the increasing in practical work skilled using OHT, Power Point, and Audio Visual media. There were 18.16 (very low), 36.39 (medium); and 49.31 (very high) respectively. The ANAVA-A test and LSDT indicated that there was significant difference between three score of the practical work skilled with three different media at three topics. The result of pre-test and post-test showed that there was the increasing score for all topics. The great increasing was enzyme topic when using Audio-visual media. The t-test indicated that there was the differentia of the increase score at three topics. The evaluation questionnaire showed that Audio-Visual media presented by LCD was effective

EFFECT OF TIME FERMENTATION OF SOYBEAN TEMPE TO TRIPSIN ACTIVITY Retno Arianingrum; Eddy Sulistyowati; Das Salirawati
Jurnal Penelitian Saintek Vol 12, No 2: Oktober 2007
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

The aim of the research is to study the effect of the duration of fermentation process toward the content of soluble protein in tempe from soybean, and the activity of trypsin enzym toward that protein.The content of soluble protein was analyzed by Lowry method using casein as solution standard, and the tripsin activity was determined by Anson method. The duration offermentation process were 0,24,48,72, 96, and 120 hours. The research indicated that the content ofsoluble protein in tempe duringfermentation process for 0, 24, 48, 72,96, and 120 hours were 0.172; 0.212; 0.217; 0.212; 0.197, and0.158% (wlw) respectively, and the activity of trypsine toward the that soluble protein were 1.35; 2.33; 2.73; 2.13; 1.17 and o. 78 unit. It was indicated that the duration of fermentation process influenced thecontent of soluble protein and tripsin activity. The highest of trypsin activity was infermentation process for 48 hours.

EFFECT OF MALELO SANDAL SALT WITH HN03 TO THE EFFICIENCY OF CRYSTAL IMPLEMENTATION Siti Sulastri; Susila Kristianingrum; Retno Arianingrum
Jurnal Penelitian Saintek Vol 9, No 1: April 2004
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

The objective of this study was to investigate the effect of soaking nitrate acid of Malelo sand to adsorption efficiency of chromium (Cr). The Malelo sand was prepared by soaking in nitric acid with variation of concentration were 17,5: 35 and 70% for 24 hours at room temperature. The adsorption efficiency of chromium was determined based on concentration of chromium residue after adsorption. which measured using Atomic Absorption Spectrophotometer (AAS). The characterization of Malelo sand determined using FTIR and XRD. The result indicated that the mean ofadsorption efficiency of chromium on Malelo sand without preparation and soaking in nitrate acid with variation concentration 17.5: 35 and 70% were 91,36: 92,40: 92,72 and 94,004% respectively.

ANTIOXIDANT ACTIVITY AND ANTIKANKER DERIVATING BENZALASETON Sri Handayani; Retno Arianingrum; Winarto Haryadi
Jurnal Penelitian Saintek Vol 18, No 1: April 2013
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Modification of vanillin structure to vanililacetone and divanililaseton and its activity test as antioxidant and anticancer have been done. The structural modification is carried out by condensation of the crosslinked aldol in an alkaline atmosphere with the aqueous ethanol solvent for 3 hours at 10 ° C. The antioxidant activity test on the synthesis compound was done by deoxyribose degradation method, while the anticancer activity test was done by MTT method. The results of antioxidant activity test show that the divanililaseton with IC50 is 873 ug / ml. more active than vanililacetone with IC50 of 11,765 ug / ml .. IC50 data for anticancer activity against cancer cell HeLa vanilinaseton and divanilinaseton respectively of 51.68 and 10.26 ug / ml. These results show that both compounds are very toxic to HeLa cancer cells.

TECHNOLOGY DEVELOPMENT OF LEATHER PRESERVATION FOR CRAFT HANDICRAFT PRODUCTS Zulfi Hendri; Retno Arianingrum
Jurnal Penelitian Saintek Vol 15, No 2: Oktober 2010
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

The purpose of this research is developing preservation technology of the Pondoh zalacca and Zalacca Gading peel for craft products material. The study begins with analyzing content, water, carbohydrates, and proteins in the peel of zalacca that is the cause of the growth of fungus and microorganisms. The next step is preservation using borax, formalin, and chlorine at concentrations: 2, 4, 6, 8, and 10%. The activity of fungal growth on the peel were observed in microscopic, and the characteristics of materials for craft products made by observing the characteristics of the peel including the texture, softness, andfracture. The results showed that the bark peel has high content of water which is 74.67% in Pondoh zalacca and 30.06% in zalacca gading. The carbohydrate content of Pondoh zalacca peel at 3.8%, and zalacca gading peel at 5.5%. Protein content at 0.565% in Pondoh zalacca and 1.815% in zalacca gading. The observation for 3 (three) months showed preservation with borax, formalin, and chlorine at all concentrations effectively prevent fungal growth. The good peel elasticity of Pondoh zalacca and Gading produced by using borax, formalin, and chlorine in the concentration of below 6%, while giving preservative chlorine above 4% make the peel a -little whiter, and preservation of over 4% borax makes chapped peel so it is difficult to be attached to the craft media. Keywords: Technology, Preservation, Zalacca peel, and Art Crafts

THE EFFECT OF p-HIDROXY m-METHOXY CHALCONE (pHmMK) ON Bcl-2 AND Bax PROTEIN EXPRESSION IN MCF-7 BREAST CANCER CELL LINES Retno Arianingrum
Jurnal Penelitian Saintek Vol 21, No 1: April 2016
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Chalcone derivate compound [3-(4’-hydroxy-3’-methoxyphenyl) -1-phenyl-2- propene-1-on)] or para hydroxyl meta methoxy chalcone (pHmMC) has been observed. It showed that it is able to induce apoptosis (cell death) in MCF-7 breast cancer cell. This study was aimed at ﬁ nding out the apoptosis mechanism pathway of pHmMC on MCF-7 cells by observing its effect on Bcl-2 and Bax proteins. The protein expression was observed by using immunocytochemistry (ICC) and analyzed using ImageJ software. The results showed that the pHmMC induce apoptosis through mechanism that decrease expression of Bcl-2 and increase expresion of Bax proteins.

Co-Authors Alya Rizkita Irwan Arbain, Cipto Aris, Asmi Ariswan Ariswan Astuti Lestari Azeeza, Sabira Nurul Bian Ihda An Naila Chaniago, Muhammad Akbar Das Salirawati Das Salirawati Eddy Sulistyowati Eddy Sulistyowati Edy Meiyanto Eli Rohaeti Fajar Naqsyahbandi Fapriyan Wijoyo Mulyopratikno Gistiana, Ilyas Hanafia, Hafiizhoh Hari Sutrisno Heri Retnawati Indyah Sulistyo Arty Isana Supiah Yosephine Louise Ixora Sartika Mercuriani Kun Sri Budiasih Latifah Kurnia Devani Marpaung, Dewi Natalia Mary Garson Masatake Niwa Masatake Niwab Mulyopratikno, Fapriyan Wijoyo Nadiya Fitri Anisa Naqsyahbandi, Fajar Nida Qurbaniah Juhara Niwa Masatake Nova Irawati Simatupang Nova Irawati Simatupang, Nova Irawati Nur Aini Purnamaningsih Nur Fitriyana Nurfina Aznam Prastika, Farera Nareswari Kirana Putri, Rizka Maylaffayza Retno Sunarminingsih Sudibyo Ridani, Maulida Rudyansah Rudyansah S. Senam Sangal, Aditi Sani, Ida Nurjelita Senam Senam Shiddiqi, Muhammad Habib Ash Siti Sulastri Siti Sulastri Sofia Mubarika Haryana Sri . Handayani Sri Atun Sri Atun Sri Atun SRI ATUN Sri Handayani Sri Handayani Sri Nurestri Sri Nurestri Susila Kristianingrum Togu Gultom Wijoyo Mulyopratikno, Fapriyan Wika Rinawati Winarto Haryadi Winarto Haryadi Zulfi Hendri

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