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All Journal Jurnal Ilmu Informasi Perpustakaan dan Kearsipan Jurnal Kesehatan Andalas IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Jukema (Jurnal Kesehatan Masyarakat Aceh) Jurnal Basicedu Jurnal EDUTECH Undiksha Jurnal Ilmu Manajemen, Ekonomi dan Kewirausahaan Jurnal Basicedu Jurnal Flyover Optimal: Jurnal Ekonomi dan Manajemen Jurnal Pelayanan dan Pengabdian Masyarakat Indonesia Heca Journal of Applied Sciences
Mifthahul Jannah
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Study of Mangan Peroxidase (Mnp) Enzymes from Pleurotus Ostreatus Produced using Coffee Grounds and Rice Bran as Substrates through Response Surface Methodology Jannah, Mifthahul; Hermansyah, Hermansyah; Hariani, Poedji Loekitowati
IJFAC (Indonesian Journal of Fundamental and Applied Chemistry) Vol 10, No 1 (2025): February 2025
Publisher : IJFAC (Indonesian Journal of Fundamental and Applied Chemistry)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24845/ijfac.v10.i1.68

Abstract

This study aimed to optimize the production of Manganese Peroxidase (MnP) enzyme from Pleurotus ostreatus using agricultural waste substrates such as coffee grounds (CG) and rice bran (RB) through Response Surface Methodology (RSM) approach. Optimization was conducted using a Central Composite Design (CCD) with three main variables, namely MnSO₄ concentration (0.5-1.5) mM, incubation temperature (25-35) °C, and reaction time (20-30) min. The enzyme fractionation process was carried out by ammonium sulfate saturation (0-20%), (20-40%), (40-60%), followed by dialysis, and SDS-PAGE analysis. The results showed that the 0-20% fraction of CG:RB (50:50) substrate had the highest enzyme activity, which was 20.588 ± 0.141 U/mL, with the highest specific activity of 76.967 ± 0.325 U/mg. The highest protein content was found in CG:RB (0:100) substrate at 0.825 ± 0.016 mg/mL. However, the enzyme activity was lower due to the presence of non-enzymatic proteins and natural inhibitors. This study successfully demonstrated that the substrate combination of CG:RB (50:50) provided optimal results for MnP production. The Response Surface Methodology proved effective in optimizing process variables, providing great potential for biotechnology applications using agricultural waste as an environmentally friendly raw material.Keywords: Pleurotus ostreatus, Manganese Peroxidase, Coffee Dregs, Rice Bran, RSM, SDS-PAGE
Enhancing TiO2 Photoelectrode Performance: The Role of La Doping Using the Sol-Gel Process Jannah, Mifthahul; Azmi, Nurul; Lala, Andi; Jalil, Zulkarnain; Mursal, Mursal
Heca Journal of Applied Sciences Vol. 3 No. 1 (2025): March 2025
Publisher : Heca Sentra Analitika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.60084/hjas.v3i1.266

Abstract

Due to growing interest in renewable energy technologies, dye-sensitized solar cells (DSSCs) have emerged as promising alternatives to conventional photovoltaics. This study explores the enhancement of titanium dioxide (TiO2) photoelectrodes through doping with lanthanum (La), a rare earth element known for its ability to modify semiconductors' structural and electronic properties. La-doped TiO2 was synthesized using the sol-gel method with La concentrations ranging from 0 to 4 mol%. The resulting films were deposited onto substrates and sintered at 600 °C for 1 hour. Characterization techniques included X-ray diffraction (XRD), scanning electron microscopy with energy-dispersive spectroscopy (SEM-EDS), UV-Vis spectrophotometry, and Fourier-transform infrared spectroscopy (FTIR). XRD confirmed the anatase phase of TiO2 and the appearance of La2O₃ phases with doping. The smallest crystallite size (8.20 nm) and lowest bandgap energy (3.31 eV) were achieved at 1 mol% La, compared to 3.52 eV for undoped TiO2. SEM-EDS indicated uniform La distribution, while FTIR revealed changes in surface chemistry. These results suggest that La doping, particularly at 1 mol%, can effectively enhance the optical and structural properties of TiO2, making it a promising candidate for improved DSSC performance.
Pengaruh Parkir di Badan Jalan Terhadap Kinerja Jalan: Studi Kasus : Mall Town Square, Jalan Perintis Kemerdekaan Makassar Jannah, Mifthahul; Badaron, Sitti Fauziah; Syarkawi, Mukhtar Thahir
Jurnal Flyover Vol. 4 No. 1 (2024): Februari - Juli
Publisher : Magister Teknik Sipil PPs UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52103/jfo.v4i1.2156

Abstract

Salah satu pusat perbelanjaan yang ada di pusat kota Makassar adalah Mall Town Square yang terletak di Jalan Perintis Kemerdekaan merupakan salah satu jalan yang termasuk jalan Nasional dan berbagai kegiatan ekonomi yang harus mampu melayani kebutuhan perparkiran pengunjung. Dengan demikian, hal ini diperkirakan akan menimbulkan suatu pergerakan yang tinggi, dan juga membutuhkan kapasitas ruang parkir yang tersedia agar tidak menimbulkan kelebihan volume parkir yang dapat membuat pengunjung harus parkir di badan jalan. Penelitian ini bertujuan untuk mengetahui kebutuhan dan ketersediaan ruang parkir serta pengaruh parkir di badan jalan terhadap kinerja ruas jalan depan Mall Town Square. Metode penelitian yang digunakan terdiri dari pengumpulan data, analisis kuantitatif dan analisis kualitatif dan bersumber dari beberapa survey ke instansi terkait dengan Mall Town Square. Hasil Penelitian menunjukkan bahwa parkir di badan jalan mempengaruhi dampak lalu lintas terhadap kinerja ruas Jalan Mall Town Square Printis Kemerdekaan.
Co-Authors Armitha Silalahi Aulia Rahma Badaron, Sitti Fauziah Cristina Efrinauli Sinaga Delmi Sulastri Dian G. Purba Elva Rahmah Emmi Juliati Sinaga Febrianti Situmorang Ferdian Admil Sandika Ferial Farouk Damanik Fernaendi Girsang Fernaendy Girsang Hapzi Ali Hermansyah Hermansyah Juwika Kristina Purba Lala, Andi Marzuki Marzuki Melda Rotua Simanjuntak Mursal Mursal Nurul Azmi, Nurul Pawer Darasa Panjaitan Poedji Loekitowati Hariani Reinita Reinita Rika Ardhana Roma Ulewi Simanjuntak Samyel Saragih Syarkawi, Mukhtar Thahir Yuniar Lestari Zulkarnain Jalil