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All Journal Jurnal Kimia (Journal of Chemistry) Buletin Udayana Mengabdi

N. L. P. V. Paramita

Program Studi Farmasi Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Udayana

Author-ID : 2921941

Chemistry Education

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OPTIMASI METODE PURIFIKASI EKSTRAK DAUN SIRIH HIJAU (Piper betle Linn) YANG MEMILIKI AKTIVITAS ANTIBAKTERI TERHADAP BAKTERI Propionibacterium Acnes W. A. Wijaya; N. L. P. V. Paramita; N. M. P. Susanti
Jurnal Kimia (Journal of Chemistry) Vol. 12 No.1 Januari 2018
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Propionibacterium acnes merupakan bakteri utama penyebab jerawat, dimana dilaporkan dalam suatu penelitian bahwa persentase ditemukannya bakteri P. acnes pada lesi jerawat sebesar 78,8%. Daun sirih hijau telah banyak dilaporkan memiliki aktivitas sebagai antibakteri. Kemampuan antibakteri daun sirih hijau disebabkan karena adanya senyawa golongan fenol yang terdiri dari kavikol, hydroxychavicol, chavibetol, estragol, eugenol, carvacrol dan golongan senyawa seskuiterpen. Penelitian ini bertujuan untuk mengetahui metode optimum yang menghasilkan aktivitas antibakteri terhadap bakteri P. acnes dari enam fraksi yang diperoleh. Metode purifikasi yang digunakan untuk mendapatkan ke-6 fraksi tersebut adalah LLE dengan penggunaan pelarut polar etanol-air yang tidak bercampur dengan pelarut heksan, kloroform dan dietileter. Ke-6 fraksi uji tersebut selanjutnya diuji aktivitas antibakterinya dengan metode difusi disk dan dilanjutkan dengan metode tambahan yaitu KLT bioautografi kontak. Analisis data yang dilakukan secara deskriptif terhadap nilai diameter zona hambat dengan mengkategorikannya berdasarkan CLSI dan terhadap hasil skrining fitokimia. Dari hasil penelitian ini diperoleh hanya dua fraksi yaitu fraksi n-heksan dan fraksi dietileter yang mampu menghambat pertumbuhan bakteri P. acnes dengan nilai diameter zona hambat sebesar 8 mm dan 9 mm. Metode purifikasi ekstrak daun sirih hijau yang merupakan gabungan metode maserasi dan LLE yang dilakukan belum optimal karena dilihat dari 6 fraksi yaitu fraksi etanol-air (FEA I), etanol-air (FEA II), etanol-air (FEA III), kloroform, dietil eter dan fraksi n- belum mampu menghambat pertumbuhan bakteri P. acnes dimana ke-6 fraksi tersebut termasuk dalam kategori resistant.

KARAKTERISTIK SIMPLISIA TEH HITAM DARI TANAMAN Camelia sinensis Var. assamica DARI PERKEBUNAN TEH BALI CAHAYA AMERTA, DESA ANGSERI, KECAMATAN BATURITI, KABUPATEN TABANAN, BALI N. L. P. V. Paramita; N. M. D. Andani; I. A. P. Y. Putri; N. K. S. Indriyani; N. M. P. Susanti
Jurnal Kimia (Journal of Chemistry) Vol.13 No.1 Januari 2019
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Black tea is a type of tea in such a way the polyphenols undergo a high degree of oxidation. The characteristics of simplicia are strongly influenced by the environment. In order to use a simplicia as an active ingredient, characterization test are required for maintaining the quality and safety. This study aimed to find out the characteristic of black tea simplicia from tea leaves (Camellia sinensis var. assamica) which were taken from Bali Cahaya Amerta Tea Plantation, Angseri Village, Baturiti Sub-district, Tabanan Regency, Bali. The characterization of the simplicia was done based on standard parameters of tea plant in Farmakope Herbal Indonesia including macroscopic test, microscopic test, the loss on drying, the contents of water soluble compound, ethanol soluble compound, total ash, acid insoluble ash, total phenol and the identification of cathecin. The results showed that black tea simplicia elongated shaped with blackish brown, odorless, tasteless, and there were fragments identifier such as sclerenkim fibers, epidermis with stomata, macrosklerida, leaf mesophyll with vascular tissue, and rosette calcium oxalate crystals. The result of the compounds content that dissolve in water was 31.84% ± 0.6446%, compounds content that dissolve in ethanol was 16.64% ± 0.414%, the loss on drying was 5.19% ± 0.0902%, total ash content was 4.67% ± 0.0392%, ash content that was insoluble in acid was 0.19% ± 0.0025%, and the total fenol content was 0.9733% ± 0.0189%. From the results, the black tea simplicia fulfills the requirements of simplicia characteristic based on Farmakope Herbal Indonesia parameter.

PENETAPAN KADAR FENOL TOTAL DAN KATEKIN DAUN TEH HITAM DAN EKSTRAK ASETON TEH HITAM DARI TANAMAN CAMELLIA SINENSIS VAR. ASSAMICA N. L. P. V. Paramita; N. P. T. W. Andari; N. M. D. Andani; N. M. P. Susanti
Jurnal Kimia (Journal of Chemistry) Vol.14 No.1 Januari 2020
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Indonesia is quite well known as one of the countries that has tea plantations and uses its own production of tea. As a tea producing country, Indonesia has been able as a tea exporter. The most tea production in Indonesia is black tea product. Black tea products are produced from species Camellia sinensis Var. Assamica which undergoes an enzymatic oxidation process to fresh tea leaves. The most phenolic compounds contained in the black tea leaf are catechins, theaflavins, and thearubigin. The purpose of this study was to determine the phenolic total and catechin content of black tea products and acetone extracts produced by D'wan Tea (DT) and Bali Cahaya Amertha (BCA) plantations which located in Tabanan district, Bali. Determination of total phenol was carried out on black tea leaf products and acetone extract using the Folin-Ciocalteu method compared with gallic acid standards. Determination of catechin content was performed on fresh (before oxidized) tea leaves, black tea leaf products, and acetone extracts using the UV-Vis Spectrophotometry method at a maximum wavelength of 257 nm. The results showed that total phenol content of D'wan Tea black tea products (SDT) and Bali Cahaya Amerta (SBCA) were 1.50 ± 0.02 % mg GAE/g dan 0.97 ± 0.02 % mg GAE/g, respectively. Results of the total phenol content of D'Awan Tea (EADT) black tea acetone extract was 6.92 ± 0.12 % mg GAE/g, while the Bali Cahaya Amerta (EABCA) black tea acetone extract was 7.38 ± 0.07 % mg GAE/g. Catechin content from fresh leaf samples of D'wan Tea (SSDT), SDT, and EADT were 15.30 ± 0.22 % mg/g, 8.37 ± 0.13 % mg/g, and 37.45 ± 0.74 % mg/g. Catechin content of fresh leaf samples of Bali Cahaya Amerta (SSBCA), SBCA, and EABCA were 14.71 ± 0.28 % mg/g, 15.16 ± 0.58 % mg/g, and 35.66 ± 0.89 % mg/g. Total phenol content of DT black tea products is greater than BCA black tea products. DT black tea products appear to have decreased catechins in processing. In the processing of black tea it is necessary to decrease catechins content due to the formation of catechin polymers, namely theaflavin and thearubigin as a determinant of the quality of black tea. Keywords: black tea, Camellia Sinensis, cathecin, total phenol.

PERBANDINGAN UJI AKTIVITAS ANTIBAKTERI MINYAK ATSIRI DAUN, TANGKAI BUNGA DAN BUNGA CENGKEH BALI (Syzygium aromaticum L.) TERHADAP BAKTERI Propionibacterium acne DENGAN METODE DIFUSI DISK I. P. S. T. Lova; W. A. Wijaya; N. L. P. V. Paramita; A. A. R. Y. Putra
Jurnal Kimia (Journal of Chemistry) Vol. 12 No.1 Januari 2018
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Propionibacterium acnes adalah bakteri anaerob gram positif yang merupakan bakteri paling dominan pada lesi jerawat. Minyak atsiri telah dibuktikan memiliki aktivitas antibakteri gram positif maupun bakteri gram negatif. Selain bunga cengkeh, minyak atsiri juga dapat diperoleh dari bagian tangkai bunga dan daun dari tanaman cengkeh. Oleh karena pemanfaatan bunga cengkeh masih terhitung mahal, maka pada penelitian ini peneliti ingin melihat potensi aktivitas antibakteri minyak atsiri dari tangkai bunga dan daun cengkeh. Tujuan penelitian ini adalah untuk membandingkan aktivitas antibakteri minyak atsiri dari bagian bunga, tangkai bunga dan daun cengkeh terhadap bakteri P.acnes. Minyak atsiri dari bunga, tangkai bunga dan daun diperoleh dengan menggunakan metode destilasi air. Konsentrasi larutan uji yang digunakan 200 ?l/mL dengan metode uji difusi disk, kontrol negatif etanol 96% v/v, kontrol positif doksisiklin 30?g/disk, media uji MHA, dan suspensi bakteri P. acnes sebesar 0,5 McFarland. Aktivitas antibakteri ditentukan berdasarkan diameter zona hambat yaitu daerah bening diantara latar keruh setelah diinkubasi selama 24 jam. Analisis data dilakukan dengan menggunakan analisis deksriptif untuk melihat klasifikasi respon hambatan berdasarkan NCCLS dan analisis statistik menggunakan ANOVA one way. Aktivitas antibakteri minyak atsiri bunga, tangkai bunga dan daun cengkeh terhadap bakteri P. acnes berbeda signifikan secara statistik dengan taraf kepercayaan >95%. Minyak atsiri bunga cengkeh menghasilkan aktivitas antibakteri terbesar yaitu 25,85 mm - 26,75 mm sedangkan minyak atsiri tangkai bunga menghasilkan aktivitas dengan zona hambat 20,60 mm - 21,20 mm dan minyak atsiri daun cengkeh menghasilkan zona hambat sebesar 18,04 mm - 18,58 mm. Hal ini membuktikan bahwa minyak atsiri dari bunga cengkeh memiliki aktivitas yang paling baik terhadap P.acnes s dibandingkan dengan minyak atsiri dari tangkai bunga dan daun cengkeh. Dengan demikian, minyak atsiri dari tangkai bunga dan daun cengkeh belum sebanding dengan yang berasal dari bunga cengkeh apabila dimanfaatkan sebagai anti bakteri P. Acnes.

PENGOLAHAN HASIL PERTANIAN BUNGA GEMITIR SEBAGAI MASKER WAJAH KAYA ANTIOKSIDAN L.P.F. Larasanty; N.L.P.V. Paramita; N.P.E. Leliqia; N.P.L. Laksmiani
Buletin Udayana Mengabdi Vol 22 No 4 (2023): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/BUM.2023.v22.i04.p05

Gemitir flower cultivation is one of the agricultural commodities in Apuan village, Susut district, Bangli. The demand for gemitir flowers fluctuates from time to time. When prices are low, farmers will generally experience losses. It is necessary to carry out community service activities that aim to diversify gemitir flower agricultural products into cosmetic products in the form of natural face masks with high antioxidant content. Gemitir flower petals are known to contain tagetin and hydroxyflavones which are naturally antioxidants and are useful in the process of skin regeneration. This face mask product can become a processed product that provides more economic value for farmer groups in Apuan Bangli Village. Community service activities are carried out in stages starting with a survey at the location of the service to see when the gemitir flowers are harvested. At harvest time, the flowers that have bloomed are then collected as needed to be used as samples for processing into face masks. In the first step, the activities carried out were the process of selecting and drying flowers in a natural way and with the help of an oven, then they were made into powder. Product manufacturing activities begin with the pre-formulation of mask preparations. Then with the existing formula, training activities on how to make masks were carried out, including mask packaging. The results of monitoring and evaluation activities showed an increase in public knowledge with an initial average value of 28 to 71 after the final monitoring evaliation was carried out. Keywords: gemitir flower, face mask, natural, antioxidant, training.

Co-Authors A. A. R. Y. Putra A.A.G.R.Y. Putra Adiluhur M. A. Anak Agung Gede Rai Yadnya Putra Budiningrum, N.W. Coky N. W. C. Dewantari A. A. I. S. H Dewi L. R. Diarini A. S. I G.A.A. Trisnadewi I Made Agus Gelgel Wirasuta I. A. P. Y. Putri I. N. K. Widjaja I. P. S. T. Lova Ketut Widyani Astuti L.P.F. Larasanty N. K. S. Indriyani N. M. D. Andani N. M. D. Andani N. M. P. Susanti N. M. P. Susanti N. P. T. W. Andari N.L.P.P. Ardiyanti N.M.P. Dwijayanti N.M.P. Susanti N.P.C. Pratiwi Ni Made Pitri Susanti Ni Putu Eka Leliqia Ni Putu Linda Laksmiani Ni Putu Sinta Mahasuari Oka M. Putra, I.M.D.S Putri I G.A.A.R.C. Rasmita, L.D. Sari, D. R. A. P. Suastini I G.A.N. Utami N.P.P. W. A. Wijaya W. A. Wijaya Widyaningtias N. M. S. R Wintari L.K.S. Wulansari, I. A. R. Yustiantara, Putu Sanna

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