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All Journal Jurnal Ilmu dan Kesehatan Hewan Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Kedokteran Hewan
Tjok Gde Oka Pemayun
Laboratorium Reproduksi Veteriner Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman Denpasar, Bali.
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : Jurnal Veteriner

 1 
Kadar dan Daya Luteolitik PGF2? Produksi Sel Monolayer Vesikula Seminalis dan Endometrium Sapi Bali (PROSTAGLANDIN F2? CONCENTRATIONS OF BALI CATTLE ENDOMETRIAL AND SEMINAL VESICLE MONOLAYER CELLS CULTURE PRODUCTS AND ITS IN VITRO TEST ON LUTEAL MONOLAYER Tjok Gde Oka Pemayun; I Gusti Ngurah Bagus Trilaksana; Laba Mahaputra
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aims of this research were to determine PGF2? concentration the produced by bali cattlesendometrial and seminal vesicle monolayer cell culture and in vitro luteolytic ability on luteal monolayercell culture. The endometrial and seminal vesicle epithelial cell of bali cattle were cultured in tissueculture medium (TCM) 199 growth medium supplemented with 10% fetal calf serum and 10% EstrusMare Serum. The cells were cultured at 1.9 x 106 density per ml medium. Then Followed by incubation at38.50 C in 5% CO2 atmosphere for 12 days. The level of PGF2? in the cell culture medium were assayed byRadioimmnuassay (RIA) technique. The luteal cells were cultured in 9 days incubation and divided into 2groups. Group I were added with 10% of cell culture product and group II were added with 1,25 mgdinoprost/ml. The level of progesterone produced by luteal cell culture was measured at day 9th and 11thincubation. The result showed concentration of PGF2? cell product of seminal vesicle cell culture wassignificantly higher (P < 0.05) compared to endometrial cell culture. There was no significant difference(P>0.05) in luteolytic ability between PGF2? cell culture product and dinoprost. In conclusion, the PGF2?could be produced by monolayer cell culture of bali cattle is endometrial and seminal vesicle epithelialcells more over they have similar ability with dinoprost in luteolytic ability.
Waktu Inseminasi Buatan yang Tepat pada Sapi Bali dan Kadar Progesteron pada Sapi Bunting (THE OPTIMUM TIME FOR ARTIFICIAL INSEMINATION IN BALI CATTLE AND THE PROGESTERONE LEVEL IN PREGNANT COW) Tjok Gde Oka Pemayun; I Gusti Ngurah Bagus Trilaksana; Made Kota Budiasa
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study aims were to determine the proper time for insemination and the progesterone level ofpregnant Bali cattle. Complete randomized design method was used in this study. The study consisted ofthree treatment groups i.e. Group I, animals were inseminated at 0 hour (on estrus), group II, inseminatedat 12 h post-estrus and group III inseminated at 24 h post-estrus. Estrus was observed two times a day ie.in the morning (6:00 to 8:00 am) and afternoon (16:00 to 18:00 pm) which was characterized by transparentvaginal discharge. The results showed that the highest percentage of pregnancy occurred when the cattlewere inseminated at 24 h post-estrus (100%), however, statistically this was not significant different (P>0.05) to animals that were inseminated at 12 h post-estrus (75%). Moreover, no pregnancy (0%) wasobserved in cattle that were inseminated at estrus. The progesterone level of pregnant bali cattle increasedas the period of gestation increased, being 15.43 ± 0.50 ng/mL at 30 days of gestation, 17.16 ± 0.34 ng/mLat 60 days of gestation and 20.78 ± 0.59 ng/mL at 90 days of gestation. In conclusion, the best time forinsemination in Bali cattle is at 24 h post-estrus and progesterone level seems to increase as the older thegestation period.
Kadar Prostaglandin F2? pada Cairan Vesikula Seminalis dan Produk Sel Monolayer Vesikula Seminalis Sapi Bali (CONCENTRATIONS OF PROSTAGLANDIN F2? IN SEMINAL VESICLE FLUID AND PRODUCT OF SEMINAL VESICLE MONOLAYER CELLS OF BALI CATTLE) Tjok Gde Oka Pemayun
Jurnal Veteriner Vol 8 No 4 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In this study, the concentration of prostaglandin F2 ? (PGF2?) in seminal vesicle fluid and seminal vesicle monolayer cell cultures of Bali cattle was determined. The seminal vesicle fluid was aspirated and the epithelial cells of the seminal vesicles were cultured in tissue culture medium (TCM) 199 growth medium containing 10% fetal calf serum (FCS) and 10% oestrus mares serum (EMS) with a density of 1.9 x 106 cells / ml medium. Following an incubation at 38.50 C in 5% CO2 atmosphere for 6 days and the level of PGF2 ? in the original seminal vesicle fluid and in the cell culture medium were determined by radioimmunoassay techniques (RIA). The results showed that the level of PGF2 ? in the non-extracted monolayer culture of seminal vesicle (1287,50 ± 3,39 pg/ml ) was significantly higher than that of detected in non-extracted seminal vesicle fluid (1,23 ± 0,79 pg/ml). In contrast, after extraction the level of PGF2 ? in seminal vesicle monolayer cell cultures (218,33 ± 2,87 pg/ml) significantly decreased as compared to seminal vesicle fluid (1750,83 ± 2,71 pg/ml). In conclusion the highest level of PGF2 ? was found in the extract of seminal vesicle fluid.
THE RESPONSE OF PGF2 ? EXTRACTION RESULT OF BALI CATTLE SEMINAL VESICLE FLUID ON PROGESTERONE LEVEL OF LUTEAL PHASE IN THE MARE Tjok Gde Oka Pemayun; Laba Mahaputra; Ismudiono -; Soetjipto -
Jurnal Veteriner Vol 9 No 4 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The study was carried out to determine the response of PGF2 ? result extraction of Bali cattle seminal vesicle fluid on the level of progesterone. The seminal vesicle fluid was aspirated and then were. extracted with methanol. This reseach was conducted by devided the mare in the luteal phase into two groups. The first group were treated with PGF2 ? result extraction of Bali cattle seminal vesicle fluid and the second group were treated with dinoprost as a patent product of PGF2 ? were administered intra uterine. The level of progesterone was measured before (0 hours) treatment and at 24, 48, 72 hours after treatment. The level of Progesterone were determined by radioimmunoassay (RIA) technique. The result showed that PGF2 ? result extraction of Bali cattle seminal vesicle liquid decreased the progesterone level 73.03% at 24 hours and 92.79% at 48 Hours. However, there was no significant different between PGF2 ? result extraction of Bali cattle seminal vesicle liquid with PGF2 ? of paten product on the progesterone level decreased. In conclusion, PGF2 ? extraction of Bali cattle seminal vesicle liquid can decreas the level of progesterone in the mare with luteal phase.
Co-Authors Aerawata, I Wayan Gede Anak Agung Sagung Kendran Gunawan, I Wayan Nico Fajar I Gusti Ngurah Bagus Trilaksana I Ketut Puja I Ketut Suardita I Nyoman Oka Widiarta I Putu Agus Kertawirawan I Wayan Bebas I WAYAN PUTRA ARIMBAWA Ismudiono - Khaza Aini Laba Mahaputra MADE KOTA BUDIASA Made Kota Busiasa Ni Ketut Suartini Pandia, Yoga Mahendra Sentana Putra Soetjipto - W. Puger