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All Journal Jurnal Ilmu dan Kesehatan Hewan Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Kedokteran Hewan
Tjok Gde Oka Pemayun
Laboratorium Reproduksi Veteriner Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman Denpasar, Bali.
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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Gambaran Hormon Progesteron Sapi Bali selama Satu Siklus Estrus ARIMBAWA, I WAYAN PUTRA; TRILAKSANA, I GUSTI NGURAH BAGUS; OKA PEMAYUN, TJOK GDE
Indonesia Medicus Veterinus Vol 1 (3) 2012
Publisher : Faculty of Veterinary Medicine, Udayana University

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Abstract

Banyaknya masalah yang dilaporkan mengenai kurang optimalnya fungsi reproduksi sapi bali sehingga dipandang perlu dilakukan penelitian lebih lanjut mengenai status reproduksi dari sapi bali tersebut. Penelitian ini bertujuan untuk mengetahui gambaran hormon progesteron sapi bali selama satu siklus estrus. Sampel berupa serum darah diambil dari sentra peternakan sapi bali Sobangan dengan menggunakan 5 ekor sapi bali yang telah pernah beranak, menunjukkan siklus estrus secara normal dan secara reproduksi dinyatakan sehat. Sampel darah diambil setiap hari dimulai dari saat munculnya estrus sampai dengan munculnya estrus kembali. Pengukuran kadar progesteron dilakukan dengan metode ELISA dan data yang diperoleh disajikan secara deskriptif dalam bentuk grafik. Hasil penelitian menunjukkan kadar progesteron pada sapi bali mulai hari ke- 0 (estrus) : 0,52 ± 0,03, mulai meningkat pada hari ke-4 pada kadar 2.03 ± 0,06, mencapai puncak pada hari ke-14 dengan kadar 9,52 ± 0,06 dan mulai menurun pada hari ke-15 dengan kadar 7,96 ± 0,07.
Induksi Berahi dengan PGF2 Alfa dan Penyuntikan Gn-RH Setelah di Inseminasi Buatan pada Sapi Bali Budiasa, Made Kota; Pemayun, Tjok Gde Oka
Indonesia Medicus Veterinus Vol 8 (5) 2019
Publisher : Faculty of Veterinary Medicine, Udayana University

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Abstract

Tujuan penelitian ini adalah untuk mengetahui munculnya berahi setelah penyuntikan PGF2 alfa dan angka kebuntingan setelah penyuntikan Gn-RH (Gonadotrophin Releasing Hormone) pada sapi bali. Materi penelitian adalah 20 ekor sapi bali betina yang sudah dua kali beranak, dan kondisi sehat serta mempunyai siklus berahi normal. Sapi-sapi penelitian dibagi menjadi dua kelompok perlakuan. Kelompok I disuntik PGF2 alfa (LutalyseTM, Pharmacia & Upjohn Company, Prizer Inc.) Sebanyak 25 mg/ml/ekor secara intramuskuler. Kelompok II disuntik PGF2 alfa 25 mg/ml/ekor secara intrauterin dengan masing – masing kelompok terdiri dari 10 kali ulangan. Sapi-sapi yang menunjukkan gejala berahi dibagi menjadi 2 kelompok. Kelompok I diinseminasi tanpa penyuntikan Gn-RH (kontrol), dan kelompok II disuntik Gn-RH 250 ug/ekor (Fertagyl, Intervet. Inc) secara intramuskuler, 3 hari setelah inseminasi buatan, dengan masing-masing kelompok terdiri dari 10 kali ulangan. Hasil penelitian menunjukan bahwa rataan munculnya berahi setelah penyuntikan PGF2 alfa secara intramuskuler adalah 65.,60 ± 8.26 jam dan 40.80 ± 6.19 jam secara intrauterin, dan secara statistik menunjukkan perbedaan yang nyata. Persentase kebuntingan pada penyuntikan Gn-RH adalah 100% dan tanpa Gn-RH (adalah 70%). Hasil penelitian ini dapat disimpulkan bahwa pemberian PGF2 alfa secara intrauterin mampu menginduksi munculnya berahi lebih cepat dan penyntikan Gn-RH mampu meningkatkan angka kebuntingan.
Motilitas dan Daya Hidup Sperma Ayam Cemani dalam Pengencer Susu Skim Fosfat pada Penyimpanan Suhu Ruang Aerawata, I Wayan Gede; Bebas, Wayan; Pemayun, Tjok Gde Oka
Indonesia Medicus Veterinus Vol 10 (1) 2021
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2021.10.1.94

Abstract

Ayam cemani merupakan unggas asli Indonesia yang berasal dari wilayah Kedu, Temanggung, Jawa Tengah. Ayam cemani mempunyai populasi dan produksi yang rendah. Penelitian ini bertujuan untuk mengetahui pengaruh penyimpanan semen terhadap motilitas dan daya hidup spermatozoa ayam cemani dengan pengencer susu skim fosfat yang disimpan pada suhu ruang atau 29°C. Rancangan yang digunakan yaitu Rancangan Acak Lengkap (RAL) dengan tujuh perlakuan dan lama waktu penyimpanan spermatozoa sebagai berikut: T0 = penyimpanan selama 0 menit; T1 = penyimpanan selama 30 menit; T2 = penyimpanan selama 60 menit; T3 = penyimpanan selama 90 menit; T4 = penyimpanan selama 120 menit; T5 = penyimpanan selama 150 menit; T6 = penyimpanan selama 180 menit. Masing-masing kelompok perlakuan dilakukan pengulangan sebanyak empat kali. Parameter yang diamati adalah motilitas progresif (%) dan daya hidup spermatozoa (%) selama 180 menit. Hasil penelitian menunjukkan penyimpanan semen ayam cemani dengan menggunakan pengencer susu skim fosfat mampu mempertahankan kualitas spermatozoa dengan waktu penyimpanan hingga 150 menit dengan motilitas progresif mencapai 41,75±1,70% dan daya hidup 47,5±1,29%. Simpulan dari hasil penelitian ini menunjukan bahwa penyimpanan semen ayam cemani menggunakan pengencer susu skim fosfat pada suhu 29°C layak digunakan untuk IB hingga 150 menit waktu penyimpanan.
Motilitas dan Daya Hidup Spermatozoa Ayam Cemani dalam Pengencer Ringer Laktat Kuning Telur yang Disimpan pada Suhu 4ºC Pandia, Yoga Mahendra; Bebas, Wayan; Pemayun, Tjok Gde Oka
Indonesia Medicus Veterinus Vol 10 (1) 2021
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2021.10.1.105

Abstract

Penelitian bertujuan untuk mengetahui pengaruh lama penyimpanan semen ayam cemani terhadap moltilitas dan daya hidup spermatozoa pada pengencer ringer laktat kuning telur yang disimpan pada suhu 4o C. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan tujuh kelompok perlakuan lama penyimpanan masing masing: T0 = penyimpanan selama 0 jam, T1= penyimpanan selama 12 jam, T2 = penyimpanan selama 24 jam, T3 = penyimpanan selama 36 jam, T4 = penyimpanan selama 48 jam, T5 = penyimpanan selama 60 jam, T6 = penyimpanan selama 72 jam. Variabel yang diamati berupa motilitas progresif (%) dan daya hidup spermatozoa (%). Motilitas diamati secara subyektif di bawah mikroskop dengan pembesaran 400x, sedangkan daya hidup diamati dengan pengecatan eosin-negrosin. Masing masing perlakuan dilakukan pengulangan sebanyak empat kali. Data yang diperoleh dianalisis dan apabila terdapat perbedaan yang nyata dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa lama penyimpanan berpengaruh nyata (p<0,05) terhadap motilitas dan daya hidup sperma. Pada penelitian ini dapat disimpulkan bawa lama penyimpanan semen ayam cemani menggunakan pengencer ringer laktat kuning telur pada suhu 4º C masih layak digunakan selama 60 jam penyimpanan dengan motilitas 46,00±0,81% dengan daya hidup 50.75±0,96%.
Kadar Estrogen dan Munculnya Estrus setelah Pemberian Buserelin (Agonis GnRH) pada Sapi Bali yang Mengalami Anestrus Postpartum Akibat Hipofungsi Ovarium Ni Ketut Suartini; I Gusti Ngurah Bagus Trilaksana; Tjok Gde Oka Pemayun
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

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Abstract

The purpose of this study was to measure the levels of estrogen and the onset of estrous after administration of Buserelin on bali cattle experiencing anestrous postpartum due to ovarian hypofunction. This research used twenty seven bali cattles which were divided evenly into three groups of treatment, namely group-one as control group (P0, without treatment), group-two, receiving a single injection of Buserelin at dose of 0.02 mg (P1) and group three, receiving repeated injection of Buserelin at dose of 0.02 mg at 24 hours interval (P3). Serum estrogen levels were measured at a time off before estrous, a time of estrous, and five days after the onset of estrous using ELISA method. Data were analyzed using statistical analysis of variance and a Duncan’s Multiple Range Test for a further analysis. The results showed that at the stage of estrous, the estrogen level of P0 was lower significantly (p<0.05) than two others treatments (P1 and P2), but those of P1 and P2 were statistically the same (p>0.05). The onset of estrous on P2 was shorter than those of P1 (p<0.05). It can be concluded that the administration of Buserelin can trigger an estrous on Bali cattle that experiencing anestrous postpartum due to ovarian hypofunction and repeated administrations of Buserelin can shorten the onset of estrous.
Bioaktivitas Gel Aloe vera pada Gonad Tikus Putih Jantan (Rattus norvegicus) I Ketut Suardita; I Ketut Puja; Tjok Gde Oka Pemayun
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

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Abstract

The purpose of this study was to determine Bioactivity of Aloe vera gel on gonad of male white rats (Rattus norvegicus). This research used complately random design, with three treatments namely T0, received placebo as a control; T1, received gel of Aloe vera 300 mg/kg body weight orally; and T2, received gel of Aloe vera 400 mg/kg body weight orally. Aloe vera gel was administrated every day for 21 days. All the treatments repeated three times, and each experimental unit used one white rat. Data found was analyzed using one way of Anova, and the Least Significant Differences (LSD) Test was applied for further analysis. Results showed that Aloe vera gel administration with a dose of 300 mg/kg body weight had effected significantly on the number of spermatogonia cells, live-daed rasio of spermatozoa, and the percentage of sperm abnormality. The result of the research indicates that Aloe vera gel is potentially cytotoxic to testes cells, and so, it’has a high possibilities as an alternative herbal contraceptive agent for animals
Kadar dan Daya Luteolitik PGF2? Produksi Sel Monolayer Vesikula Seminalis dan Endometrium Sapi Bali (PROSTAGLANDIN F2? CONCENTRATIONS OF BALI CATTLE ENDOMETRIAL AND SEMINAL VESICLE MONOLAYER CELLS CULTURE PRODUCTS AND ITS IN VITRO TEST ON LUTEAL MONOLAYER Tjok Gde Oka Pemayun; I Gusti Ngurah Bagus Trilaksana; Laba Mahaputra
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aims of this research were to determine PGF2? concentration the produced by bali cattlesendometrial and seminal vesicle monolayer cell culture and in vitro luteolytic ability on luteal monolayercell culture. The endometrial and seminal vesicle epithelial cell of bali cattle were cultured in tissueculture medium (TCM) 199 growth medium supplemented with 10% fetal calf serum and 10% EstrusMare Serum. The cells were cultured at 1.9 x 106 density per ml medium. Then Followed by incubation at38.50 C in 5% CO2 atmosphere for 12 days. The level of PGF2? in the cell culture medium were assayed byRadioimmnuassay (RIA) technique. The luteal cells were cultured in 9 days incubation and divided into 2groups. Group I were added with 10% of cell culture product and group II were added with 1,25 mgdinoprost/ml. The level of progesterone produced by luteal cell culture was measured at day 9th and 11thincubation. The result showed concentration of PGF2? cell product of seminal vesicle cell culture wassignificantly higher (P < 0.05) compared to endometrial cell culture. There was no significant difference(P>0.05) in luteolytic ability between PGF2? cell culture product and dinoprost. In conclusion, the PGF2?could be produced by monolayer cell culture of bali cattle is endometrial and seminal vesicle epithelialcells more over they have similar ability with dinoprost in luteolytic ability.
Waktu Inseminasi Buatan yang Tepat pada Sapi Bali dan Kadar Progesteron pada Sapi Bunting (THE OPTIMUM TIME FOR ARTIFICIAL INSEMINATION IN BALI CATTLE AND THE PROGESTERONE LEVEL IN PREGNANT COW) Tjok Gde Oka Pemayun; I Gusti Ngurah Bagus Trilaksana; Made Kota Budiasa
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study aims were to determine the proper time for insemination and the progesterone level ofpregnant Bali cattle. Complete randomized design method was used in this study. The study consisted ofthree treatment groups i.e. Group I, animals were inseminated at 0 hour (on estrus), group II, inseminatedat 12 h post-estrus and group III inseminated at 24 h post-estrus. Estrus was observed two times a day ie.in the morning (6:00 to 8:00 am) and afternoon (16:00 to 18:00 pm) which was characterized by transparentvaginal discharge. The results showed that the highest percentage of pregnancy occurred when the cattlewere inseminated at 24 h post-estrus (100%), however, statistically this was not significant different (P>0.05) to animals that were inseminated at 12 h post-estrus (75%). Moreover, no pregnancy (0%) wasobserved in cattle that were inseminated at estrus. The progesterone level of pregnant bali cattle increasedas the period of gestation increased, being 15.43 ± 0.50 ng/mL at 30 days of gestation, 17.16 ± 0.34 ng/mLat 60 days of gestation and 20.78 ± 0.59 ng/mL at 90 days of gestation. In conclusion, the best time forinsemination in Bali cattle is at 24 h post-estrus and progesterone level seems to increase as the older thegestation period.
Kadar Prostaglandin F2? pada Cairan Vesikula Seminalis dan Produk Sel Monolayer Vesikula Seminalis Sapi Bali (CONCENTRATIONS OF PROSTAGLANDIN F2? IN SEMINAL VESICLE FLUID AND PRODUCT OF SEMINAL VESICLE MONOLAYER CELLS OF BALI CATTLE) Tjok Gde Oka Pemayun
Jurnal Veteriner Vol 8 No 4 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In this study, the concentration of prostaglandin F2 ? (PGF2?) in seminal vesicle fluid and seminal vesicle monolayer cell cultures of Bali cattle was determined. The seminal vesicle fluid was aspirated and the epithelial cells of the seminal vesicles were cultured in tissue culture medium (TCM) 199 growth medium containing 10% fetal calf serum (FCS) and 10% oestrus mares serum (EMS) with a density of 1.9 x 106 cells / ml medium. Following an incubation at 38.50 C in 5% CO2 atmosphere for 6 days and the level of PGF2 ? in the original seminal vesicle fluid and in the cell culture medium were determined by radioimmunoassay techniques (RIA). The results showed that the level of PGF2 ? in the non-extracted monolayer culture of seminal vesicle (1287,50 ± 3,39 pg/ml ) was significantly higher than that of detected in non-extracted seminal vesicle fluid (1,23 ± 0,79 pg/ml). In contrast, after extraction the level of PGF2 ? in seminal vesicle monolayer cell cultures (218,33 ± 2,87 pg/ml) significantly decreased as compared to seminal vesicle fluid (1750,83 ± 2,71 pg/ml). In conclusion the highest level of PGF2 ? was found in the extract of seminal vesicle fluid.
THE RESPONSE OF PGF2 ? EXTRACTION RESULT OF BALI CATTLE SEMINAL VESICLE FLUID ON PROGESTERONE LEVEL OF LUTEAL PHASE IN THE MARE Tjok Gde Oka Pemayun; Laba Mahaputra; Ismudiono -; Soetjipto -
Jurnal Veteriner Vol 9 No 4 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The study was carried out to determine the response of PGF2 ? result extraction of Bali cattle seminal vesicle fluid on the level of progesterone. The seminal vesicle fluid was aspirated and then were. extracted with methanol. This reseach was conducted by devided the mare in the luteal phase into two groups. The first group were treated with PGF2 ? result extraction of Bali cattle seminal vesicle fluid and the second group were treated with dinoprost as a patent product of PGF2 ? were administered intra uterine. The level of progesterone was measured before (0 hours) treatment and at 24, 48, 72 hours after treatment. The level of Progesterone were determined by radioimmunoassay (RIA) technique. The result showed that PGF2 ? result extraction of Bali cattle seminal vesicle liquid decreased the progesterone level 73.03% at 24 hours and 92.79% at 48 Hours. However, there was no significant different between PGF2 ? result extraction of Bali cattle seminal vesicle liquid with PGF2 ? of paten product on the progesterone level decreased. In conclusion, PGF2 ? extraction of Bali cattle seminal vesicle liquid can decreas the level of progesterone in the mare with luteal phase.
Co-Authors Aerawata, I Wayan Gede Anak Agung Sagung Kendran Gunawan, I Wayan Nico Fajar I Gusti Ngurah Bagus Trilaksana I Ketut Puja I Ketut Suardita I Nyoman Oka Widiarta I Putu Agus Kertawirawan I Wayan Bebas I WAYAN PUTRA ARIMBAWA Ismudiono - Khaza Aini Laba Mahaputra MADE KOTA BUDIASA Made Kota Busiasa Ni Ketut Suartini Pandia, Yoga Mahendra Sentana Putra Soetjipto - W. Puger