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All Journal Jurnal Ilmu dan Kesehatan Hewan Buletin Veteriner Udayana Jurnal Veteriner Indonesia Medicus Veterinus Jurnal Kedokteran Hewan

Tjok Gde Oka Pemayun

Laboratorium Reproduksi Veteriner Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman Denpasar, Bali.

Author-ID : 2925606

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Gambaran Hormon Progesteron Sapi Bali selama Satu Siklus Estrus ARIMBAWA, I WAYAN PUTRA; TRILAKSANA, I GUSTI NGURAH BAGUS; OKA PEMAYUN, TJOK GDE
Indonesia Medicus Veterinus Vol 1 (3) 2012
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (334.061 KB)

Banyaknya masalah yang dilaporkan mengenai kurang optimalnya fungsi reproduksi sapi bali sehingga dipandang perlu dilakukan penelitian lebih lanjut mengenai status reproduksi dari sapi bali tersebut. Penelitian ini bertujuan untuk mengetahui gambaran hormon progesteron sapi bali selama satu siklus estrus. Sampel berupa serum darah diambil dari sentra peternakan sapi bali Sobangan dengan menggunakan 5 ekor sapi bali yang telah pernah beranak, menunjukkan siklus estrus secara normal dan secara reproduksi dinyatakan sehat. Sampel darah diambil setiap hari dimulai dari saat munculnya estrus sampai dengan munculnya estrus kembali. Pengukuran kadar progesteron dilakukan dengan metode ELISA dan data yang diperoleh disajikan secara deskriptif dalam bentuk grafik. Hasil penelitian menunjukkan kadar progesteron pada sapi bali mulai hari ke- 0 (estrus) : 0,52 ± 0,03, mulai meningkat pada hari ke-4 pada kadar 2.03 ± 0,06, mencapai puncak pada hari ke-14 dengan kadar 9,52 ± 0,06 dan mulai menurun pada hari ke-15 dengan kadar 7,96 ± 0,07.

Induksi Berahi dengan PGF2 Alfa dan Penyuntikan Gn-RH Setelah di Inseminasi Buatan pada Sapi Bali Budiasa, Made Kota; Pemayun, Tjok Gde Oka
Indonesia Medicus Veterinus Vol 8 (5) 2019
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.615 KB)

Tujuan penelitian ini adalah untuk mengetahui munculnya berahi setelah penyuntikan PGF2 alfa dan angka kebuntingan setelah penyuntikan Gn-RH (Gonadotrophin Releasing Hormone) pada sapi bali. Materi penelitian adalah 20 ekor sapi bali betina yang sudah dua kali beranak, dan kondisi sehat serta mempunyai siklus berahi normal. Sapi-sapi penelitian dibagi menjadi dua kelompok perlakuan. Kelompok I disuntik PGF2 alfa (LutalyseTM, Pharmacia & Upjohn Company, Prizer Inc.) Sebanyak 25 mg/ml/ekor secara intramuskuler. Kelompok II disuntik PGF2 alfa 25 mg/ml/ekor secara intrauterin dengan masing – masing kelompok terdiri dari 10 kali ulangan. Sapi-sapi yang menunjukkan gejala berahi dibagi menjadi 2 kelompok. Kelompok I diinseminasi tanpa penyuntikan Gn-RH (kontrol), dan kelompok II disuntik Gn-RH 250 ug/ekor (Fertagyl, Intervet. Inc) secara intramuskuler, 3 hari setelah inseminasi buatan, dengan masing-masing kelompok terdiri dari 10 kali ulangan. Hasil penelitian menunjukan bahwa rataan munculnya berahi setelah penyuntikan PGF2 alfa secara intramuskuler adalah 65.,60 ± 8.26 jam dan 40.80 ± 6.19 jam secara intrauterin, dan secara statistik menunjukkan perbedaan yang nyata. Persentase kebuntingan pada penyuntikan Gn-RH adalah 100% dan tanpa Gn-RH (adalah 70%). Hasil penelitian ini dapat disimpulkan bahwa pemberian PGF2 alfa secara intrauterin mampu menginduksi munculnya berahi lebih cepat dan penyntikan Gn-RH mampu meningkatkan angka kebuntingan.

Motilitas dan Daya Hidup Sperma Ayam Cemani dalam Pengencer Susu Skim Fosfat pada Penyimpanan Suhu Ruang Aerawata, I Wayan Gede; Bebas, Wayan; Pemayun, Tjok Gde Oka
Indonesia Medicus Veterinus Vol 10 (1) 2021
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2021.10.1.94

Ayam cemani merupakan unggas asli Indonesia yang berasal dari wilayah Kedu, Temanggung, Jawa Tengah. Ayam cemani mempunyai populasi dan produksi yang rendah. Penelitian ini bertujuan untuk mengetahui pengaruh penyimpanan semen terhadap motilitas dan daya hidup spermatozoa ayam cemani dengan pengencer susu skim fosfat yang disimpan pada suhu ruang atau 29°C. Rancangan yang digunakan yaitu Rancangan Acak Lengkap (RAL) dengan tujuh perlakuan dan lama waktu penyimpanan spermatozoa sebagai berikut: T0 = penyimpanan selama 0 menit; T1 = penyimpanan selama 30 menit; T2 = penyimpanan selama 60 menit; T3 = penyimpanan selama 90 menit; T4 = penyimpanan selama 120 menit; T5 = penyimpanan selama 150 menit; T6 = penyimpanan selama 180 menit. Masing-masing kelompok perlakuan dilakukan pengulangan sebanyak empat kali. Parameter yang diamati adalah motilitas progresif (%) dan daya hidup spermatozoa (%) selama 180 menit. Hasil penelitian menunjukkan penyimpanan semen ayam cemani dengan menggunakan pengencer susu skim fosfat mampu mempertahankan kualitas spermatozoa dengan waktu penyimpanan hingga 150 menit dengan motilitas progresif mencapai 41,75±1,70% dan daya hidup 47,5±1,29%. Simpulan dari hasil penelitian ini menunjukan bahwa penyimpanan semen ayam cemani menggunakan pengencer susu skim fosfat pada suhu 29°C layak digunakan untuk IB hingga 150 menit waktu penyimpanan.

Motilitas dan Daya Hidup Spermatozoa Ayam Cemani dalam Pengencer Ringer Laktat Kuning Telur yang Disimpan pada Suhu 4ºC Pandia, Yoga Mahendra; Bebas, Wayan; Pemayun, Tjok Gde Oka
Indonesia Medicus Veterinus Vol 10 (1) 2021
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2021.10.1.105

Penelitian bertujuan untuk mengetahui pengaruh lama penyimpanan semen ayam cemani terhadap moltilitas dan daya hidup spermatozoa pada pengencer ringer laktat kuning telur yang disimpan pada suhu 4o C. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan tujuh kelompok perlakuan lama penyimpanan masing masing: T0 = penyimpanan selama 0 jam, T1= penyimpanan selama 12 jam, T2 = penyimpanan selama 24 jam, T3 = penyimpanan selama 36 jam, T4 = penyimpanan selama 48 jam, T5 = penyimpanan selama 60 jam, T6 = penyimpanan selama 72 jam. Variabel yang diamati berupa motilitas progresif (%) dan daya hidup spermatozoa (%). Motilitas diamati secara subyektif di bawah mikroskop dengan pembesaran 400x, sedangkan daya hidup diamati dengan pengecatan eosin-negrosin. Masing masing perlakuan dilakukan pengulangan sebanyak empat kali. Data yang diperoleh dianalisis dan apabila terdapat perbedaan yang nyata dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa lama penyimpanan berpengaruh nyata (p<0,05) terhadap motilitas dan daya hidup sperma. Pada penelitian ini dapat disimpulkan bawa lama penyimpanan semen ayam cemani menggunakan pengencer ringer laktat kuning telur pada suhu 4º C masih layak digunakan selama 60 jam penyimpanan dengan motilitas 46,00±0,81% dengan daya hidup 50.75±0,96%.

Kadar Estrogen dan Munculnya Estrus setelah Pemberian Buserelin (Agonis GnRH) pada Sapi Bali yang Mengalami Anestrus Postpartum Akibat Hipofungsi Ovarium Ni Ketut Suartini; I Gusti Ngurah Bagus Trilaksana; Tjok Gde Oka Pemayun
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.489 KB)

The purpose of this study was to measure the levels of estrogen and the onset of estrous after administration of Buserelin on bali cattle experiencing anestrous postpartum due to ovarian hypofunction. This research used twenty seven bali cattles which were divided evenly into three groups of treatment, namely group-one as control group (P0, without treatment), group-two, receiving a single injection of Buserelin at dose of 0.02 mg (P1) and group three, receiving repeated injection of Buserelin at dose of 0.02 mg at 24 hours interval (P3). Serum estrogen levels were measured at a time off before estrous, a time of estrous, and five days after the onset of estrous using ELISA method. Data were analyzed using statistical analysis of variance and a Duncan’s Multiple Range Test for a further analysis. The results showed that at the stage of estrous, the estrogen level of P0 was lower significantly (p<0.05) than two others treatments (P1 and P2), but those of P1 and P2 were statistically the same (p>0.05). The onset of estrous on P2 was shorter than those of P1 (p<0.05). It can be concluded that the administration of Buserelin can trigger an estrous on Bali cattle that experiencing anestrous postpartum due to ovarian hypofunction and repeated administrations of Buserelin can shorten the onset of estrous.

Bioaktivitas Gel Aloe vera pada Gonad Tikus Putih Jantan (Rattus norvegicus) I Ketut Suardita; I Ketut Puja; Tjok Gde Oka Pemayun
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.684 KB)

The purpose of this study was to determine Bioactivity of Aloe vera gel on gonad of male white rats (Rattus norvegicus). This research used complately random design, with three treatments namely T0, received placebo as a control; T1, received gel of Aloe vera 300 mg/kg body weight orally; and T2, received gel of Aloe vera 400 mg/kg body weight orally. Aloe vera gel was administrated every day for 21 days. All the treatments repeated three times, and each experimental unit used one white rat. Data found was analyzed using one way of Anova, and the Least Significant Differences (LSD) Test was applied for further analysis. Results showed that Aloe vera gel administration with a dose of 300 mg/kg body weight had effected significantly on the number of spermatogonia cells, live-daed rasio of spermatozoa, and the percentage of sperm abnormality. The result of the research indicates that Aloe vera gel is potentially cytotoxic to testes cells, and so, it’has a high possibilities as an alternative herbal contraceptive agent for animals

Profil Glukosa Darah Dan Urea Plasma Pada Sapi Bali Yang Menderita Anestrus Post Partum (THE BLOOD GLUCOSE PROFILE AND PLASMA UREA IN POSPARTUM ANESTRUS OF BALI CATTLE) Made Kota Busiasa; Tjok Gde Oka Pemayun
Buletin Veteriner Udayana Vol. 7 No. 1 Pebruari 2015
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (168.441 KB)

The aim of this study was to determine the levels of blood glucose and plasma urea in postpartum anestrus bali cattle. The research design used to determine both blood glucose andblood plasma urea concentration is completely randomized design consisting of two groups: groupI, cows have a normal cycle and group II, postpartum anestrus cows experiencing with each groupconsisting of 4 repetitions . The results in the study showed that glucose levels did not appear toshow significant differences between glucose levels during estrus and cattle anestrus postpartum. While the plasma urea levels appear to show significant differences between urea levels duringestrus and cattle anestrus postpartum. Time significantly for glucose on day- 0 and the day to-14.The same thing seems urea levels that increase significantly on day- 0 and to 14. The results canbe concluded that urea levels influence the emergence of postpartum estrus in bali cattle, whereasglucose levels had no effect.

Perkembangan Folikel dan Munculnya Estrus setelah Penyuntikan GnRH pada Sapi Bali yang Mengalami Anestrus Postpartum dengan Body Condition Score Berbeda I Nyoman Oka Widiarta; Tjok Gde Oka Pemayun; I Gusti Ngurah Bagus Trilaksana
Buletin Veteriner Udayana Vol. 12 No. 1 Pebruari 2020
Publisher : The Faculty of Veterinary Medicine, Udayana University

This study aim to determine the development of follicles and onset of estrus due to GnRH induction in bali cattle which has anestrus postpartum more than three month with differnt body condition score in the Badung regency of Bali.The designd used in this study was a complete random design were divided into three treatment groups, namely Group 1 : cattle with BCS 2, Group 2 : cattle with BCS 3, Group 3 : cattle with BCS 4, each group consisted of 9 replication. All of group of bali cattle are induced by GnRH (Fertagyl, Intervet,Inc) at a dose of 500µg/IM/cattle. The result of this study indicate the development of follicles averages before induction of GnRH is 4.03 + 0.21 mm, 4.60 + 0.18 mm, 4.56 + 0.22 mm, each for BCS2, BCS 3 and BCS 4 and development of follicles averages after induction of GnRH is 7.08 + 0.42mm, 11.06 + 0.40 mm and 11.99 + 0.33 each for BCS 2, BCS 3 and BCS 4 and statistically shows that there are significant differences (P<0,05). The averages onset of estrus in the three treatment group is 7.75 + 0.89 day, 4.63 + 0.52 day dan 3.63 + 0.52day, each for BCS 2, BCS 3 and BCS 4 and statistically shows that there are significant differences (P<0,05). The largest follicular diameter in BCS 4 is 11,99 + 0,33 mm with the time of onset of estrus 3,63 + 0,52 day. The result can be concluded that the induction of GnRH 500ug in bali cattle can increase follicular development and accelerate the oset of estrous.

Efektivitas Prostaglandin F2? dalam Menginduksi Berahi, Non Return Rate dan Conception Rate pada Sapi Bali Anestrus Postpartum I Putu Agus Kertawirawan; I Gusti Ngurah Bagus Trilaksana; Tjok Gde Oka Pemayun
Buletin Veteriner Udayana Vol. 13 No. 2 Agustus 2021
Publisher : The Faculty of Veterinary Medicine, Udayana University

Induction of heat is one of the technologies in the field of reproduction to accelerate the appearance of heat in cattle. Many methods are used to induction of heat, one of them is by giving prostaglandin F2?. This study aims to determine the effectiveness of the administration of PGF2? in bali cattle anestrus postpartum with corpus luteum functioning and different body weights to induce the appearance of heat, non-return rate, and conception rate after insemination once and twice at 12-hour intervals. This research was conducted using a randomized block design based on body weight and the number of insemination. Group I (P1) is a group with a bodyweight of less than 250 kg and received one insemination when the appearance of lust, Group II (P2) with a bodyweight of less than 250 kg and received two inseminations at 12-hour intervals, group III (P3) with body weights above 250 kg with one insemination and group IV (P4) with body weights above 250 kg and received twice insemination at 12-hour intervals. Statistical analysis and testing are done by the analysis of variance. The results showed that the administration of PGF2? caused the emergence of estrus in all experimental animals and there was no significant difference (P> 0.05) to the appearance of heat between the four treatment groups, and the insemination treatment twice intervals 12 hours also had no significant effect to enhance the number of non-return rate and conception rate.

Pengukuran Folikel Ovarium dan Temperatur Vagina Sapi Bali yang Mengalami Silent Tjok Gde Oka Pemayun; Anak Agung Sagung Kendran; I Wayan Nico Fajar Gunawan
Buletin Veteriner Udayana Vol. 13 No. 1 Pebruari 2021
Publisher : The Faculty of Veterinary Medicine, Udayana University

This study aimed to improve Bali cattle's efficiency and productivity by measuring ovarian follicles and vaginal temperature in Bali cattle that experienced a silent heat using ten bali cattle with normal estrus and bali cattle that experience a silent heat. The research samples were divided into two groups: Bali cattle that showed normal estrus (grup I) and Bali cattle that experienced a silent heat (grup II), with each group consisted of 5 replications. Furthermore, all the cows were measured for vaginal measurements at the time of estrus and 24 hours after estrus. While ovarian follicles are measured at the time of estrus. The results showed that the average vaginal temperature of Bali cattle was 38.40 ± 0.130 C and 38.04 ± 0.130 C respectively for Bali estrus cattle and Bali cattle which experienced a silent heat and statistically showed a significant difference (P<0.05). The Bali cattle follicles' average diameter is 10.60 ± 1.14 and 7.32 ± 0.37 mm, respectively, for the normal estrus and which experience a silent heat, and statistically show a significant difference (P<0.05). From the results of the study, it can be concluded that the vaginal temperature of bali cattle with normal estrus is higher than that of bali cattle which experience a silent heat, and ovarian follicle diameter in normal estrus is greater than the diameter of the ovarian follicles of bali cattle that experience silent heat.

Co-Authors Aerawata, I Wayan Gede Anak Agung Sagung Kendran Gunawan, I Wayan Nico Fajar I Gusti Ngurah Bagus Trilaksana I Ketut Puja I Ketut Suardita I Nyoman Oka Widiarta I Putu Agus Kertawirawan I Wayan Bebas I WAYAN PUTRA ARIMBAWA Ismudiono - Khaza Aini Laba Mahaputra MADE KOTA BUDIASA Made Kota Busiasa Ni Ketut Suartini Pandia, Yoga Mahendra Sentana Putra Soetjipto - W. Puger

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