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All Journal E-Jurnal Medika Udayana ISM (Intisari Sains Medis) : Jurnal Kedokteran Bali Anatomy Journal Indonesian Andrology and Biomedical Journal
Made Oka Negara
Departemen Andrologi, Fakultas Kedokteran Universitas Udayana
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology

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Post-Preparation Total Motile Sperm Count in Infertile Men and The Success Rate of Intrauterine Insemination at Rumah Sakit Kasih Ibu Denpasar in 2022 Wibawa, I Kadek Sadam; I Gusti Ngurah Pramesemara; I Made Oka Negara; I Gusti Ayu Widianti
Indonesian Andrology and Biomedical Journal Vol. 5 No. 1 (2024): June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/iabj.v5i1.53777

Abstract

Background: Every married couple hopes to have children, but not all can fulfil that desire. A married couple finds themselves unable to achieve pregnancy after 12 months of regular unprotected sexual intercourse, which can be called infertility. Infertility can also occur in men. In dealing with infertility, intrauterine insemination (IUI) is a cost-effective and non-invasive Assisted Reproductive Technology (ART) procedure. The condition of male infertility can be detected through semen analysis. One of the parameters in semen analysis is the total motile sperm count (TMSC). Objective: This research seeks to establish the relationship between post-preparation TMSC and the success rate of the IUI procedure. Methods: This research adopts a descriptive-analytical design employing a cross-sectional methodology. Non-probability sampling was utilized as the sampling technique, and the data analyzed were secondary data from the medical records of male patients aged 25 to 45 years old diagnosed with infertility. The subjects had undergone IUI at Rumah Sakit Kasih Ibu Denpasar from January 1 to December 31, 2022. Results: From the 35 obtained samples, 12 individuals (34.3%) had a post-preparation TMSC of ≥10 í— 106, while 23 individuals (65.7%) had a post-preparation TMSC of <10 í— 106. The success rate of the Intrauterine Insemination (IUI) procedure was 17.1%, with 4 couples (33.3%) having a post-preparation TMSC of ≥10 í— 106 and 2 couples (8.7%) with a post-preparation TMSC of <10 í— 106. The Fisher's Exact test carried out has obtained a result (p=0.151), while the value of the odds ratio (OR) obtained (OR=5.250) Conclusion: Based on statistical analysis, it can be concluded that there is no significant association between post-preparation TMSC in infertile men and the success rate of IUI procedures (p > 0.05). The odds ratio (OR) obtained is 5.250 (95% CI 0.799-34.496).
The Association of E-Cigarettes with Hypogonadism in Adult Men in Denpasar Djuanda, Louis Felix; I Gusti Ngurah Pramesemara; I Made Oka Negara; Jaqueline Sudiman; Faisal Yusuf Azhari
Indonesian Andrology and Biomedical Journal Vol. 5 No. 2 (2024): December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/iabj.v5i2.65652

Abstract

Background : A decrease in testosterone level can cause a set of symptoms forming a clinical syndrome called hypogonadism. One of the factors that is known to disrupt testosterone levels is smoking habits. Currently, the use of e-cigarettes as an alternative product to conventional cigarettes is increasing rapidly in many countries, including Indonesia. The amounts of harmful substances in e-cigarette aerosols are similar to the smoke produced by conventional cigarettes, but the health hazards of e-cigarettes are still not well known. Objective : This study was conducted to determine the relationship between e-cigarettes and hypogonadism in adult men in the city of Denpasar. Method: This study uses a descriptive-analytical method in the form of a cross-sectional study. The research sample consisted of 49 e-cigarette users collected using consecutive sampling techniques. The data were then analyzed using SPSS® version 26. Results : Based on the research obtained from the ADAM score from a total of 49 samples, the prevalence of hypogonadism is higher in the group of people who smoked e-cigarettes, about 35,3%. Overall, this study found a fairly high prevalence of hypogonadism (26.5%) in Denpasar. The chi-square test shows a p-value of 0,032 and a prevalence ratio (PR) of 5.294. Conclusions : Active use of e-cigarettes showed a significant association with the incidence of hypogonadism experienced by adult men in Denpasar. Based on the PR calculation, the risk of hypogonadism in active e-cigarette users was 5.294 times higher than that of adult men who were not active e-cigarette users.
The Effect of Arak Bali Administration on Spermatogenic Cell Counts in Wistar Rats: A Preliminary Study Nudu, Stephanus Rahardian Hersacra; I Gusti Ngurah Pramesemara; I Made Oka Negara; I Gusti Ayu Dewi Ratnayanti
Indonesian Andrology and Biomedical Journal Vol. 6 No. 2 (2025): December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/iabj.v6i2.83948

Abstract

Background: Male infertility is widely recognized as major health problem, contributing to around 50% infertility cases. Chronic excessive alcohol consumption has been found to negatively affect sperm quality. Arak Bali, a traditional spirit from Indonesia, is widely consumed, yet its specific impact on the process of spermatogenesis remains scientifically uninvestigated. Objective: This study aimed to evaluate the effects of chronic Arak Bali administration on the counts of spermatogonia, primary spermatocytes, and spermatids in male Wistar rats. Methods: This study utilized a post-test only control group design. Fourteen male Wistar rats were analyzed, allocated into a Control group (n=6) which received aquades, and an Intervention group (n=8) which was administered Arak Bali (40% v/v) daily for 42 days. For each rat, the average cell count from three seminiferous tubules was used as the representative value. Group data were compared using an independent T-test, with significance set at p < 0.05. Results: Spermatogonia, primary spermatocytes, and spermatids showing no statistically significant differences between the Control and Intervention groups for the mean counts of spermatogonia (p = 0.162), primary spermatocytes (p =0.571), or spermatids (p = 0.628). Conclusion: Chronic administration of 40% v/v Arak Bali at 0.5mL per rat per day did not significantly show a reduction in cell counts in spermatogonium, primary spermatocytes, and spermatid in Wistar rats.
The Effect of Waste Cooking Oil Administration on Spermatogenic Cells in Male Mice (Mus musculus) Sari, Dewa Ayu Candranita Kerthy; I Made Oka Negara; I Gusti Ngurah Pramasemara
Indonesian Andrology and Biomedical Journal Vol. 6 No. 2 (2025): December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/iabj.v6i2.81306

Abstract

Background : The high consumption of fried foods in Indonesia leads to the common practice of repeatedly using cooking oil, transforming it into waste cooking oil (in Indonesian called “minyak jelantah). This oil undergoes chemical changes that generate compounds like reactive oxygen species (ROS), which are known to induce oxidative stress and are implicated in male infertility by disrupting spermatogenesis. Objective : This study aimed to investigate the effect of waste cooking oil administration on the number of spermatogenic cells (spermatogonia, primary spermatocytes, and spermatids) in the testes of male mice (Mus musculus). Methods : This experimental study used a post-test-only control group design. Fourteen male BALB/c mice were randomly divided into a control group (n=7), administered 0.2 ml/day of aquades, and a intevention group (n=7), administered 0.2 ml/day of waste cooking oil (palm oil heated 8 times at 180°C) for 36 days via oral sonde. After the intervention period, the mice were euthanized, and their testes were extracted for histological preparation with Hematoxylin-Eosin (H&E) staining. The number of spermatogenic cells was counted under a microscope across four fields of view. Data were analyzed using an Independent T-test. Results : Consecutively mean number of the spermatogonia, prymary spermatocyte and spermatids in the control group are 51,98 ± 1,60; 64,50 ± 2,14; 81,31 ± 3,04. Meanwhilem, Consecutively mean number of the spermatogonia, prymary spermatocyte and spermatids in the intervention group are 28,93 ± 3,20; 39,31 ± 3,57; 45,25 ± 3,97. Which mean, the mean number of spermatogenic cells was significantly lower in the intervention group compared to the control group Conclusion : The administration of waste cooking oil significantly reduces the population of spermatogenic cells including spermaogonia, prymary spermatocyte, and spermatid cells in male mice, indicating a detrimental effect on spermatogenesis. Keywords : Waste Cooking Oil, Spermatogenic Cells, Spermatogenesis, Oxidative Stress.
Co-Authors Djuanda, Louis Felix Faisal Yusuf Azhari Geitha Puspita Darmi I Gusti Ayu Dewi Ratnayanti I Gusti Ayu Widianti I Gusti Made Aman I Gusti Ngurah Pramasemara I Gusti Ngurah Pramesemara I Wayan Weta Ida Ayu Dewi Dhyani Ida Ayu Gde Wahyudevi Dharmika J. A. Pangkahila Jaqueline Sudiman Jivitha Munusamy Made Ayu Rusanti Ni Nyoman Ayu Dewi Nudu, Stephanus Rahardian Hersacra Putu Prathiwi Kemala Putri Sosiawan Sari, Dewa Ayu Candranita Kerthy Tifania Melucha Miguel Leong Wibawa, I Kadek Sadam Wimpie I Pangkahila Yukhi Kurniawan Yukhi Kurniawan Yukhi Kurniawan