Article Per Year (5 Year)
p-Index From 2021 - 2026
0.61
P-Index
This Author published in this journals
All Journal Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) JSFK (Jurnal Sains Farmasi & Klinis) Journal of Stem Cell Research and Tissue Engineering Narra J Proceedings Book of International Conference and Exhibition on The Indonesian Medical Education and Research Institute
Adisti Dwijayanti
Departemen Ilmu Farmasi Kedokteran, FK Universitas Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Veterinary

Published : 5 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 5 Documents
Search

 1 
Follicular Development of Aged Rats Ovarian Injected Human Umbilical Cord Mesenchymal Stem Cells Resti Rahma Dianti; Alif Iman Fitrianto; Adkhilni Utami; Wining Astini; Adisti Dwijayanti; Frans Dhyanagiri Suyatna; Kelvin Yaprianto; Indra Bachtiar; Aryani Sismin Satyaningtijas; Adi Winarto; Arief Boediono
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 4 No. 1, JANUARY 2020
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v4i1.6889

Abstract

Female reproductive system showing the fastest signs of aging. The ovarian aging characterized by a decrease in follicular development. Stem cells are undifferentiated cells and can form a variety of different cells as the foundation of tissues and organs. Previous studies reported that Bone Marrow Mesenchymal Stem Cells (BM-MSCs) transplantation can restore follicular development in damaged ovarian rats. This study aimed to analyze the number of follicular development in aged rats and to analyze the capability of human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs) to improving follicular development in aged rats. This study used 3 mature rats (4 months old), and 9 nine aged rats (22-24 months old), Spraque Dawley (SD) strain. They were divided into four groups. The first and the second group was mature rats and aged rats without injection. The third and the fourth group was aged rats injected hUC-MSCs dose 106 cells/kgBW and hUC-MSCs dose 107 cells/kgBW. The injection carried out 4 times at 3-month intervals. The parameters observed were follicular development and homing image of hUC-MSCs in ovarian tissue. The results showed that the number of follicular developments in aged rats 22-24 months decreased significantly compared to mature rats 4 months old. Injection of hUC-MSCs at dose 106 cells/kgBW and 107 cells/kgBW did not increase follicular development in aged rats. hUC-MSCs did not found in ovarian tissue. It could be concluded that aged rats 22-24 months old no longer productive indicated from the number of follicular developments and corpus luteum decreased. The injection of hUC-MSCs intravenously did not indicate an improvement of follicular development in aged rats 22-24 months old.
Perubahan Parameter Biokimia, Histopatologi Ginjal Tikus Spraque Dawley Pascahipoksia Oleh Ekstrak Akar Acalypha indica dan Herba Centella asiatica Nurfitri Nurfitri; Erni Hernawati Purwaningsih; Vivian Soetikno; Adisti Dwijayanti; Novi Silvia Hardiany
Jurnal Sains Farmasi & Klinis Vol 5, No 3 (2018): J Sains Farm Klin 5(3), Desember 2018
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (731.281 KB) | DOI: 10.25077/jsfk.5.3.160-168.2018

Abstract

Hipoksia kronik merupakan salah satu penyebab penyakit ginjal akibat peningkatan pembentukan Reactive Oxygen Species (ROS)  dalam sel. Kombinasi ekstrak akar Acalypha indica 250 mg/KgBB (AI250) dan Centella asiatica 150 mg/kgBB (CA150) memiliki efek neuroterapi pada tikus Spraque Dawley pascahipoksia. Penelitian dilakukan untuk membuktikan manfaat kombinasi ekstrak etanol dan/atau ekstrak tunggalnya dapat memperbaiki kerusakan ginjal tikus pascahipoksia melalui mekanisme antioksidan. 28 tikus jantan dikelompokkan dalam 7 kelompok: kontrol normal; kontrol hipoksia+air; hipoksia+(AI200+CA150); hipoksia+(AI250+CA100); hipoksia+AI250; hipoksia+CA150; hipoksia+vitamin C. Hipoksia selama 7 hari dalam hypoxic chamber berisi O2 10% dan N2 90%, 1 atm. Setiap kelompok diberi perlakuan selama 7 hari. Pada akhir studi hewan diterminasi. Darah dan organ ginjal diambil untuk pemeriksaan biokimia dan histopatologi.Kombinasi  (AI250+CA100) menurunkan kadar MDA ginjal dan plasma secara bermakna dibandingkan kontrol hipoksia (p=0,001 dan p=0,021) dan AI250 (p=0,003 dan 0,043). Kombinasi AI250+CA100 terjadi penurunan ekspresi relatif mRNA HIF-1α (p=0,014), kadar urea plasma (p=0,001) dan perbaikan lesi intra-glomerulus p=0,013.Kesimpulan: Kombinasi (AI250+CA100) dan tunggal AI250 memiliki aktivitas antioksidan terbaik dalam mencegah kerusakan ginjal pascahipoksia, secara biokimiawi dan histopatologinya.
Beetroot (Beta vulgaris) potential in preventing colorectal cancer using in-silico analysis Dwijayanti, Adisti; Azizah, Norma N.; Erlina, Linda; Kusmardi, Kusmardi; Ningsih, Sri S.; Fadilah, Fadilah; Hashim, Najihah M.
Narra J Vol. 5 No. 2 (2025): August 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i2.1578

Abstract

Colorectal cancer (CRC) is one of the leading causes of cancer-related mortality worldwide, necessitating the need for an effective therapeutic strategy. Beta vulgaris (beetroot) possesses active compounds that exert anti-cancer properties. The aim of this study was to evaluate the potential of beetroot as a preventative agent against the progression of CRC using differentially expressed gene (DEG) analysis and network pharmacology approaches. The protein-protein interaction network and molecular docking analyses were employed to assess the key interactions of beetroot active compounds with CRC-related target protein. Cytotoxicity of beetroot extract was experimentally evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay on the HT29 cell line. The result of this study showed that protein in the cell cycle was significantly enriched in CRC, with cyclin-dependent kinase 4 (CDK4) gene as one of the specific genes. Quercetin, galangin, hesperidin, farrerol, and betanin were the most typical compounds of beetroot based on the Comparative Toxicogenomics Database (CTD). Molecular docking studies revealed the strong binding affinity between quercetin (-7.04 kcal/mol) and bentanin (-8.11 kcal/mol) with CDK4. Beetroot demonstrated anticancer properties against the HT29 cell line with IC50 value of 39.03±1.4 µg/mL. In conclusion, the beetroot extract has inhibitory activity against HT29 cell line proliferation, highlighting its potential in preventing the development of CRC through the substantial suppression of gene expression within the cell cycle pathway.
Brain Derived Neurotrophic Factor Levels in Aged Rats Post-Systemic Human Mesenchymal Stem Cell Administration dwijayanti, adisti
Journal of Stem Cell Research and Tissue Engineering Vol. 2 No. 2 (2018): JOURNAL OF STEM CELL RESEARCH AND TISSUE ENGINEERING
Publisher : Stem Cell Research and Development Center, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.863 KB) | DOI: 10.20473/jscrte.v2i2.11895

Abstract

Brain-Derived Neurotrophic Factor (BDNF) levels were affected by aging. Brain BDNF levels were known to decrease along with advanced age thus correlated with any diseases such as cognitive impairment and Alzheimer. Mesenchymal Stem Cell (MSC) is one of the potential modalities actively investigated against age-related diseases. This study evaluated the effect of human MSC administration to brain BDNF levels in aged rats. Intravenous injection of 10 million per body weight human MSC were given four times in 3 months interval to 22-24 months old female and male Spraque–Dawley rats. As control group, aged rats were injected by normal saline at the same volume and frequencies. Moreover, young 3-6 months rats also examined as negative control.  By the end of the experiment, we analyzed three rats from each group. Brain BDNF levels were measured by enzyme-linked immunosorbent assay and normalize to the protein levels. One-way ANOVA and LSD post hoc analysis was performed to compare the differences between groups. BDNF levels in male appeared similar between young, aged, and MSC treated groups. Meanwhile, control aged female groups had significantly lower BDNF levels compared to young (p = 0.019) and MSC-treated aged rats (p = 0.001). There was no difference of BDNF levels between young and MSC-treated aged in female rats (p = 0,068). Both sex had similar BDNF levels (p = 0.249) in control-aged groups. In contrast, female young and MSC-treated aged rats achieved significantly higher BDNF levels (p = 0.009 and p <0.001) compared to the male groups, respectively. These results suggest that human mesenchymal stem cell intravenous injection can increase brain BDNF levels in female aged rats.
Quantitative Flavonoids and Antioxidant Profiles by DPPH Assay of Centella Asiatica Serum Cosmetic Products Versus Ethanol Extract Habibie, Sutan Farrell; Dwijayanti, Adisti; Fachri, Wilzar
Proceedings Book of International Conference and Exhibition on The Indonesian Medical Education Research Institute Vol. 9 No. - (2025): Proceedings Book of International Conference and Exhibition on The Indonesian M
Publisher : Writing Center IMERI FMUI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69951/proceedingsbookoficeonimeri.v9i-.326

Abstract

The cosmetic industry is currently leaning toward herbal skincare products due to strong consumer preference for natural ingredients. Centella asiatica (CA) is frequently used as it contains flavonoids and centelloids known for their beneficial antioxidant properties. This study aimed to assess the efficacy of CA extract when incorporated into cosmetic formulations by comparing the total flavonoid content (TFC) and antioxidant activity of five commercial serums (A-E) against a pure CA ethanol extract. The experimental study was conducted in vitro, utilizing the CA extract, Vitamin C (as a positive control), and the five commercial serum products. TFC was determined quantitatively using UV-Visible spectrophotometry and quercetin standard. Antioxidant activity was measured using DPPH assay to calculate the IC50 value. Pure CA extract showed a mean TFC of 37.10 ± 0.21 mg QE/g, with serum samples showing high variability; the highest being Sample C with a TFC of 216.6 (99.89 - 246.1) mg QE/g and the lowest being sample E with a TFC of 24.75 ± 1.88 mg QE/g. Crucially, the pure CA extract demonstrated strong antioxidant activity with a mean IC50 of 52.45 ± 4.96 ppm. In contrast, all serum products exhibited significantly weaker antioxidant activities. Samples C and D resulted in extremely high IC50 values of 244100 ± 116700 ppm and 319300 ± 95700 ppm respectively, categorized as lower than weak, while samples A, B, and E were unable to reach 50% inhibition, indicating an antioxidant capacity below the measurable range. In conclusion, despite the pure Centella asiatica ethanol extract showing robust antioxidant activity, the commercial cosmetic serums demonstrated a significantly lower range of activity and highly inconsistent TFC, suggesting that the raw extract's potency does not reliably transfer to the final product formulation.
Co-Authors A.S. Satyaningtijas Achmad Zaki Maulidzy Adi Winarto Adkhilni Utami Adkhilni Utami Agnes Frethernety Alif Iman Fitrianto Alif Iman Fitrianto Anggi Pohan Arief Boediono Azizah, Norma N. Desak Gede B. Krisnamurti Elpita Tarigan Erlina, Linda Erni H Purwaningsih Erni H. Purwaningsih Erni H. Purwaningsih Erni Hernawati Purwaningsih, Erni Hernawati Fachri, Wilzar Fadilah Fadilah, Fadilah Frans Dhyanagiri Suyatna Frans Dhyanagiri Suyatna Frans Dhyanagiri Suyatna Habibie, Sutan Farrell Hashim, Najihah M. Hibattuloh, Ilham Abid Indra Bachtiar Iqbal, Yuri Annisa Kelvin Yaprianto Kelvin Yaprianto Ketut Adnyane Mudite Kusmardi Kusmardi Melva Louisa Novi S. Hardiany Novi Silvia Hardiany Nurfitri Nurfitri Putri, Feristia Audia Rani Wardani Hakim Resti Rahma Dianti Sandy Qlintang Sari, Grace Natalia Siti Farida Sri S. Ningsih, Sri S. Vivian Soetikno Wining Astini Wining Astini Zada, Nadzira