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Journal : Berkala Perikanan Terubuk

IDENTIFICATION OF LACTIC ACID BACTERIA (BAL) FROM BIOFLOC from TECHNOLOGY WITH TAPIOCA CARBON SOURCES IN RED TILAPIA IN CULTIVATION MEDIA (Oreochromis sp.) Suci Wulandari; Henni Syawal; Iesje Lukistyowati; Iskandar Putra
Berkala Perikanan Terubuk Vol 47, No 2 (2019): Juli 2019
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (382.588 KB) | DOI: 10.31258/terubuk.47.2.158-164

Abstract

This study aims to determine the types of lactic acid bacteria grown at different pH from floc (clots) which are given carbon sources of tapioca and can increase the number of bacteria in the medium of red tilapia cultivation. The method used is the survey method and experiment by growing bacteria on different pH treatments, namely pH 2, 4 and 6, growing colonies were identified based on morphological and biochemical characteristics. The results of identification found 13 isolates of lactic acid bacteria (BAL) which only grew at pH 4 and 6 of these isolates consisted of the genus Bacillus and Streptococcus. The total bacteria in the cultivation medium ranged from 2x108 to 7x108 CFU / mL.
TREATMENT OF Pangasionodon hypophthalmus INFECTED BY BACTERIA Edwarsiella tarda WITH SOLUTION OF APEL MANGO LEAF( Mangifera indica ) Atika Talitha Erman; Henni Syawal; Iesje Lukistyowati
Berkala Perikanan Terubuk Vol 49, No 2 (2021): Juli 2021
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/terubuk.49.2.945-956

Abstract

Apel Mango leaf is a natural ingredient that can be used as an alternative medicine because it has phytochemical content in the form of tannins, flavonoids, saponins and alkaloids. This study aims to analyze the effect of giving a solution of apel mango leaf (Mangifera Indica) in the treatment of Pangasionodon hypophthalmus, and get the best dose for the treatment of diseases caused by E. tarda bacteria, seen from total leukocytes , leukocyte differentiation, phagocytic activity and survival of Pangasionodon hypophthalmus. This study uses a completely randomized design (CRD) with 5 treatments and 3 replications. The dosage of apel mango leaf solution used was Kn (not infected and not treated) Kp (infected and not treated), P1 (1.2%), P2 (1.4%) and P3 (1.6%) . Immersion is carried out in 5 liters of water with a mixture of mango leaf solution in accordance with different doses for 5 minutes 3 times soaking interval of 2 days , then maintained for 14 days after treatment . The results showed that soaking with apel mango leaf solution significantly affected the image of leukocytes of P. hypophthalmus (P <0.05) . Best dose contained in treatment P2 (1.4%) with an average total leukocytes ( 8,33 × 104 cells / mm 3 ) , differentiation of leukocytes (lymphocytes 84.33%, monocytes 9,00 and 6,67 neutrophils %), phagocytic activity ( 36.00% ) and survival rate of Pangasionodon hypophthalmus ( 86.67% ) . 
IDENTIFICATION OF LACTIC ACID BACTERIA FROM BIOFLOK TECHNOLOGY WHICH HAS BEEN GAVE MOLLASES ON RED TILAPIA (Oreochromis sp.) AQUACULTURE Nelli Karolina Sitorus; Iesje Lukistyowati; Henni Syawal; Iskandar Putra
Berkala Perikanan Terubuk Vol 47, No 1 (2019): Februari 2019
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.013 KB) | DOI: 10.31258/terubuk.47.1.83-92

Abstract

The aim of this research was to  determine the types of lactic acid bacteria from biofloc technology with the addition of mollase in red tilapia aquaculture containers and to determine the optimum growth of lactic acid bacteria at pH 2, 4, and 6, and also to observe the density of bacteria in the biofloc container water during maintenance. The methods used in this research are survey and experiments by taking flok from maintenance container and then identifying the lactic acid bacteria. The results of lactic acid bacteria inoculation obtained 16 isolates isolated from floc which can only grow at pH 4 and pH 6. Identification based on physical tests, chemical tests, and biochemical tests showed that the isolates were classified into Bacillus and Streptococcus. The amount of bacterial density water from containers of biofloc technology were given mollase during research reached the highest point in the third week, which averaged 109 CFU/mL.
HEMOTOLOGICAL OF Clarias gariepinus THAT WERE GIVEN WITH VACCINE TO PREVENT Motile Aeromonas Septicemia DISEASE Deny Triyatna; Iesje Lukistyowati; Morina Riauwaty
Berkala Perikanan Terubuk Vol 47, No 3 (2019): NOVEMBER 2019
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (469.128 KB) | DOI: 10.31258/terubuk.47.3.217-223

Abstract

The vaccine is one of the alternative to improve infection of the immunity system. The purpose of this research was to understand the hematological of (Clarias gariepinus) that were with given Aeromonas hydrophila vaccine. This research was conducted on 14 April until 2 June 2019. The method was an experimental method with a Completely Randomized Design (CRD) five treatments and three times replication. The treatments appliced were: Kn: (The fishes that were not given with vaccine and infected by Aeromonas hydrophila), Kp: (The fishes that were not given vaccine and was infected by Aeromonas hydrophila, P1: Soaked with vaccine dose 1 ml/10 L, P2: Soaked with vaccine dose 1 ml/10 L, P3: (Dipping with vaccine dose 1 ml/10 L. Dipping with the vaccine is done for 15 minutes. Clarias gariepinus at average 8-10 cm TL. After 23 day, the fishes were infected with Aeromonas hydrophila, the purpose of which was to determine imumunity of fish. The results show that the vaccine for 15 minutes is able to prevent the Motile Aeromonas Septicemia (MAS) disease. The were range total erythrocyte is 2,29×106 sel/mm3, hemoglobin 12,60 g/dl, Leukocytes Differensial (Lymphocytes 71,33%, Monocytes 16,66% and Neutrophils 12,00%) and Survival Rate 63,3%.