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All Journal Jurnal Veteriner Berkala Perikanan Terubuk Jurnal Ilmu Lingkungan Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Tapian Nauli : Jurnal Penelitian Terapan Perikananan dan Kelautan Unri Conference Series: Community Engagement
Iesje Lukistyowati
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Agriculture, Biological Sciences & Forestry Humanities Earth & Planetary Sciences Environmental Science Industrial & Manufacturing Engineering Social Sciences Veterinary Other

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Journal : Jurnal Veteriner

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Pelacakan Gen Aerolysin dari Aeromonas hydrophila pada Ikan Mas yang Diberi Pakan Ekstrak Bawang Putih (DETECTION OF AEROLYSIN GEN FROM AEROMONAS HYDROPHILA IN COMMON CARP FED WITH GARLIC EXTRACT) Iesje Lukistyowati; Kurniasih .
Jurnal Veteriner Vol 13 No 1 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Aeromonas hydrophila is a gram negative and opportunistic bacteria, which could cause fish mortalityin a short time from 80%-100%. One virulent factor of A. hydrophila on common carp (Cyprinus carpio L)that could cause fish mortality is aerolysin. This research used a synthetic primers of oligonukleotide todetect aerolysin, a specific genomes of A. hydrophila on common carp (Cyprinus carpio L). The commoncarps have been feed a woof that contain garlic extract during 30 days before they challenged with A.hydrophila. Polymerase Chain Reaction (PCR) was used to detect an aerolysin gen from A. hydrophila. Theelectrophoresis result showed aerolysin gene of Aeromonas hydrophila from Veterinary Faculty of GadjahMada University (FKH-UGM) isolate was amplified with 462 bp of molecule weight. While the aerolysingen was detected in the fish kidney with 900 bp of molecule weight. Further, DNA sequence analysis of thePCR product of A. hydrophila from FKH – UGM isolate showed homolog with isolate A. hydrophila subsphydrophila ATCC 7966 complete genome with score 55.4 (71%).
Co-Authors Aan Deni Masrudy Abd. Rasyid Syamsuri Adelina Adelina Ahmad Sabri Ahmad Sabri Alfian ' Amraini Fitri Anzila Rizki W Muharram Aras Mulyadi Atika Talitha Erman Ayu Lestari Hasibuan Chairani Irawan Chandra Chandra Dea Switzelin H Dede Kurnia Islami Deny Triyatna Dessy Yoswaty Dian Fitria M Fabri Putra Nugraha Feliatra Feri Yulistia Hanna Pardede Henni Syawal Ilham Fajri Pohan Juandi Riki Umbara Siagian Jumaida ' Kurniasih . Lola Pitaloka br Barus M. Rizki Maulana Mardiana Siregar Margaret Viviani Mayda Susana Nelli Karolina Sitorus Niken Ayu Pamukas Nur Asiah Nursyirwani, Nursyirwani Pebri Radayani Saragih Rebeka Valensia Rini Mastuti Rubianto ' Sarbaini ' Suci Wulandari T. Ersti Yulika Sari Tri Emrinelson Wiwik Yuliana Yulyawati Yulyawati Yunita Sari Yusni Ikhwan Siregar