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All Journal JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Akademika Biologi Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Media Farmasi Indonesia
Endang Kusdiyantini
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Medicine & Pharmacology Other

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OPTIMASI PEMBERIAN PUPUK GRAMAFIX DALAM DEGRADASI CEMARAN MINYAK BUMI OLEH BAKTERI INDIGENOUS SECARA IN VITRO Linda Safitri; Agung Suprihadi; Endang Kusdiyantini; Yeti Darmayati
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Petroleum pollution has bad impact for life in marine ecosystems, particularly in coastal areas. One method of handling the pollution is bioremediation using bacteria that are able to degrade and utilize petroleum hydrocarbons as carbon source. One technique of bioremediation is biostimulation, that is the addition of nutrients that can improve the process of oil degradation by degrading bacteria. Nutrient slow-release fertilizer are used, one of which is Gramafix. The purpose of this study was to determine the optimal concentration of Gramafix in increasing degradation of petroleum contaminants by indigenous bacteria. Four concentrations of Gramafix as treatments are P1 (0,085 g), P2 (0,171 g), P3 (0,341 g) and P4 (0,682 g), as well as the negative control treatment (no fertilizer and bacteria) and positive control (with bacteria, without fertilizer). Observations were carried out  four times, on 0, 7, 14 and 28 days of incubation. The parameters used are heavy oil using the gravimetric method, the total number of bacterial cells using Acridine Orange Direct Counting and environmental factors such as nitrogen contents, phosphorus contents, temperature, dissolved oxygen, pH and salinity. Analysis of the data using a completely randomized design (CRD) with ANOVA and Duncan test. The results showed the P3 can improve the process of oil degradation by bacteria that degrade the highest percentage of 65,91% in the 28 days of incubation, it also has more bacterial cells than other treatments. The result of this study is the addition of 0,341 g Gramafix is optimum for enhancing the degradation of petroleum hydrocarbons by degrading bacteria. Keywords: Pollution, petroleum, bioremediation, biostimulation, slow-release fertilizer.
ANALISIS MIKOFLORA DALAM MAKANAN FERMENTASI TRADISIONAL KEMPONG DI DESA KARANGPUCUNG KIDUL, LINGGAPURA BUMIAYU JAWA TENGAH Devia Kusmawati Arfina; Endang Kusdiyantini; MG Isworo Rukmi
Jurnal Akademika Biologi Vol. 2 No. 1 Januari 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Kempong is a traditional fermented foods which is traditionally made of palm kernel cake substrate from the South Karangpucung Linggapura Bumiayu village, Central Java. This study is aimed to identify a mold which has a role in the fermentation process and testing in an activity of Kempong’s enzyme from mycoflora obtained. Proximate analysis of the samples of mold and palm kernel cake are conducted to determine the nutrient content of the substrate and fermentation products. The Results isolation, from the environment, substrate, laru and product, show 14 isolates of  molds, there are R.oryzae, A. niger Van Tieghem, A. carbonarius, Geotrichum candidum, A. ochraseus, Rhizomucor sp, A. chevalieri, A. tamarii, A. oryzae, A. flavus, A. nidulans, A. fumigatus, A. glaucus, and A. parasiticus. R. oryzae is a mold found in every material examined. It indicates that the fermentation is done mainly soothers by    R. oryzae. Proximate analysis of the kempong, shows a levels of carbohydrate 16.67%, 74.03% water, 0.75% ash, 2.80% fat and 5.77% protein. Nutrients content except water are lower than the substrate palm kernel cake. Decreasing of protein, fat, and carbohydrate fermentation are caused by R. oryzae. Keywords: kempong, mycoflora, enzyme activity, proximate analysis.
ISOLASI DAN IDENTIFIKASI MOLEKULER KHAMIR DARI MOLASE SERTA KEMAMPUANNYA DALAM PRODUKSI ETANOL Fauziah Citra Rahmawati; Endang Kusdiyantini; Anto Budiharjo
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The need of petroleum is increasing while the availability is limited. Petroleum is an alternative energy source which is produced by fermentation using a sugar-containing substrate with the help of yeast. This research aims to isolate the yeast that can ferment molasses into ethanol and identify morphologically, biochemically, and molecular so the species can be known. The isolation is done into the PDA media by streak method. Morphological identification involves macroscopic and microscopic observation. Biochemical identification involves growth tests on 50% glucose concentration and sugar fermentation. Molecular identification is done by looking at its genome based on Internal Transcribed Spacer (ITS) DNA base order. From Molasses isolation was obtained four isolates of yeast, that are isolates 1C1, 1C2, 2C1, and 3C2. Isolates 2C1 which is used for ethanol fermentation produces 0,9876 g/cm3 or 9,5% of ethanol. 2C1 Isolates was amplified using PCR and Phylogenetic Analysis using Neighbor Joining method. Sequencing results show that Isolates 2C1 has 616 bp Query Length. Based on Homology equation through BLAST method and phylogenetic tree analysis, isolates 2C1 is a Debaryomyces Hansenii.Keywords: Identification, Yeast, Molasses, Molecular, Ethanol
OPTIMASI PERTUMBUHAN DAN POTENSI ANTAGONISTIK Bacillus pumilus TERHADAP PATOGEN Xanthomonas campestris SERTA IDENTIFIKASI MOLEKULER GEN PENYANDI PKS DAN NRPS Laila Nur Faizah; Anto Budiharjo; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Xanthomonas campestris is one of the broccoli pathogen that difficult to be eradicated. B. pumilus is a prospective biocontrol agent due to its ability to produce antibiotical subtances that inhibit the growth of pathogenic bacteri and fungi. Beside that, B. pumilus have a great endurance to extreme environment by its ability to form spores. Some species of Bacillus genus have been reported to have PKS (Polyketide Synthetase) and NRPS (Non-Ribosomal Peptide Synthetase) coding gene which is a mega shyntetase that have a role in the production of antibiotic subtances. This reseach aims to test the inhibitory potency of B. pumilus toward X. campestris, identify the PKS and NRPS coding gene and to knwing the optimum growth of B. pumilus on the medium with different pH and carbon source. Reseach is conducted with Completely Randomized Design (CRD) with three replications on two growth parameters that is pH and carbon source. pH treatment is conducted with pH 5, 6, 7, and 8, while carbon source treatment is conducted with glucose, fructose, and sucrose as the carbon source. The obtained result is analized with ANOVA with significant level of 95%. The result shows B. pumilus ability to inhibits the growth of X. campestris producing inhibitory zone with 1,84 mm of diameter. The reseach showed negative result in NRPS coding gene detection and a positive result in PKS coding gene. PKS coding gene identification which is analized with BLAST shows 98% homology with PKS coding gene of Bacillus subtilis HNS005. Growth optimization shows the optimum groth of B. pumilus at pH 6 and glucose as the carbon source.Keywords: biocontrol, B. pumilus, PKS, NRPS, growth optimization
PRODUKSI INULINASE DAN KECEPATAN PERTUMBUHAN SPESIFIK ISOLAT KHAMIR DUCC Y-015 PADA BERBAGAI KONSENTRASI TEPUNG UMBI DAHLIA Dani Ika Purwaningsih; Arina Tri Lunggani; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 2 No. 3 Juli 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Inulin is an abundant compound in the nature after starch. Hydrolytic enzyme called inulinase is able to catalyse hydrolysis reaction of inulin polysaccharides into fructose and fructooligosaccharide (FOS). Yeast isolateDUCC Y-015 is one of the microbes that is able to produceinulinase. The action of inulinase enzyme associated with the growth of the isolate. Specific growth rate is one of the measurement of growth parameters; itis defined as the increase in cell biomass per unit time in the growth phase of the microbes. This study aims to examine theinulinase production and specific growth rate of yeast isolate DUCC Y-015 on the medium with various concentrations of dahlia tuber flour. The measurement of growth was done by weighing the cell dry weight and inulinase activity test was done by calculating the reduction sugar using DNS method. The used concentrations of dahlia tuber flourin the medium were 2%, 3%, 4% and 5%. Inulinase activity in each treatment was 0,111 IU/ml, 0,147 IU/ml, 0,113 IU/ml and 0,119 IU/ml, respectively, whereas the specific growth rate was 0,0062 hour-1, 0,0081 hour-1, 0,0077 hour-1, 0,0052 hour-1, respectively. The resultswere tested using the Mann-Whitney Test and theKruskal-Wallis Test. According to the results, the most optimuminulinase production and specific growth rate (µ) of yeast isolate DUCC Y-015 on dahlia tuber flour medium was treated with 3% concentration of dahlia tuber flour.Keywords: Dahlia Tuber Flour, Yeast Isolate DUCC Y-015, Specific Growth Rate, Inulinase Activity
IDENTIFIKASI KANDUNGAN SENYAWA FITOKIMIA MINYAK BIJI MIMBA (Azadirachta Indica, A. Juss) Dyah Palupi; Endang Kusdiyantini; Rully Rahadian; A Heru Prianto
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Neem seeds oil (Azadirachta indica, A. Juss) have phytochemical compounds which act as antifeedant, repellent, antibacterial, and antifungal. The neem seeds oil were important identified their phytochemical compounds. Identifications were done with screening phytochemical and infrared spectrophotometer. Neem seed oils were obatained with pressing method. The group of compounds tested were alkaloid, flavonoid, triterpenoid, steroid, saponin and tannin. The results of phytochemical showed that neem seeds oil consist of alkaloid, flavonoid, triterpenoid, steroid, saponin and tannin. This results were strengthned with spectrum in infrared spectrophotometer that showed the functional groups of each those compounds. Keywords: neem seeds oil, phytochemical compounds, pressing method, functional groups
ISOLASI DAN KARAKTERISASI MORFOLOGI KOLONI BAKTERI ASOSIASI ALGA MERAH (RHODOPHYTA) DARI PERAIRAN KUTUH BALI Aninditia Sabdaningsih; Anto Budiharjo; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 2 No. 2 April 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Kutuh Beach in Bali is an area established as the Minapolitan algae with a high diversity. Microorganisms associated with marine organisms, usually have secondary metabolites that can be used as a source of drugs, antibiotics, enzymes, and cosmetics. The aimed of this research was to isolate and characterize colony morphology of bacteria associated with red algae based on colony morphology. Seven bacteria were isolated from three samples of red algae that were Kappapycus alvarezii, Gelidiella acerosa and Eucheuma spinosum. The isolates had pigmentation of beige, white and orange. Based on Gram's staining, seven isolates were Gram positive with bacilli and cocci in shape. Keywords : associated bacteria, morphological colony, pigmentation, red algae
IDENTIFIKASI ISOLAT MONASCUS SP. HASIL ISOLASI ANGKAK BERDASARKAN GEN INTERNAL TRANSCRIBED SPACER (ITS) DAN PENGUKURAN KANDUNGAN PIGMEN Mia Tri Wardani; Endang Kusdiyantini; Anto Budiharjo
Jurnal Akademika Biologi Vol. 6 No. 2 April 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The Identification of Monascus based on their morphological characteristic, this methods is very problematic due to several  specimens share similarity on morphology and colours. Nowadays, the identification of molecular based organisms has been done to complement morphological identification, for example mold identification using the Internal Transcribed Spacer (ITS) gene. The objectives of study were to identify of Monascus isolates the result of angkak isolation using Internal Transcribed Spacer (ITS) gene, phylogenetic analyzing,  determination cell growth and determination of production pigment content. Sequences of  ITS Monascus were amplified using PCR and the molecular phylogenetic  analyses was using Neighbor Joining (NJ) method. Based on homology search by Basic Local Alignment Search Tool (BLAST) program and phylogenetic tree analyses, mold of isolates were identified as the Monascus purpureus (100%). The amplified DNA fragments were about 553 bp. Determination of Monascus mold cell growth were carried out by the method of dry weight cell. Determination of pigment by using spectrophotometer at 390 nm wavelength for yellow pigment and 500 nm for red pigment. The result of determination of Monascus mold pigment content show that have increased during fermentation time until day 13. The amount of yellow extracellular pigment production is 37.358 U / g; While the red color pigment of 2.6545 U / g. The amount of red intracellular pigment production is 7.4175 U / g, while the yellow pigment is 30.176 U / g.Keywords: Monascus, angkak, molecular phylogenetic, pigment
Pertumbuhan Mikroalga Botryococcus braunii Sebagai Penghasil Lipid Pada Medium Campuran Antara Air Kelapa Dan Air Laut Bintoro Rudi Saputro; Endang Kusdiyantini; Hermin Pancasakti Kusumaningrum
Jurnal Akademika Biologi Vol. 4 No. 4 Oktober 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Energy needs in fuel sector tend to increase for supporting human life. Green microalgae (Chlorophyceae) can be used as an alternative solutions  for bioenergy.  Botryococcus braunii is lipid producer microalgae which can be processed further into biodiesel. Microalgae growth medium such as Walne can be subtituted with coconut water that reducing economical cost. This research aims to determine the growth of B. braunii using coconut water and its effect on lipid production. The experiment conducted into five treatments of coconut water consist of  P0 (0%), P1 (10%), P2 (7.5%), P3 (5%), and P4 (2.5%) for five days incubation and three repetitive step. Microalgae growth was calculated according to cell count using haemocytometer. The results showed that 2.5% coconut water subtitution exhibited the best growth rate and lipid production, ie the amount of 547 cells / ml and produced lipid level according to these treatment increased 179% comparing with control. Keywords: Growth, Botryococcus braunii, Lipid, Coconut water, Seawater.
AKTIVITAS ENZYM SELULASE YANG DIHASILKAN OLEH BAKTERI Serratia marcescens PADA SUBSTRAT JERAMI Khrisna Lazuardi Budi; W Wijanarka; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 7 No. 1 Januari 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Cellulose (EC 3.2.1.4) is enzyme complex consisting of some enzymes which together decomposing cellulose into glucose by hydrolizes the β-1,4 bond in cellulose. The purpose of this study is to determine cellulose activity which produced by Serratia marcescens in different substrate concentration and at the time of incubation T4, T8, T12. This research uses Randomized Block Design (RBD) factorial pattern with two factors. The first factor was variation of straw substrate which had been delignificated (V0, V1, V2, V3). The second factor is the variation of time incubation (T4, T8, T12). Each factor is repeated 3 times. The data obtained were analyzed using Analysis  of  Variance (ANOVA)  (α  =  0.05). The result  shown that  variation concentration  of straw,  and the interaction (combination) between the straw substrate and the incubation time substrate was not significantly different. The result treatment of incubation time was significantly different of the cellulase activity. The result of anova analyzed is obtained that F count(α = 0.05) value from straw substrate, interaction (combination) between the straw substrate and the incubation time substrate, and incubation time was 0.53; 2.18; 8.00. F table(α = 0.05) value of straw substrate, interaction (combination) between the straw substrate and the incubation time substrate, and incubation time was 2.99; 2.20; 3.39. The result of anova, is continued by BNT 5% test. The result of BNT test shown that the highest incubation time of cellulase activity was in incubation time 12 hours with the average value 0.26 U/mL. Key Word : cellulose,  Serratia marcescens,  straw substrate, incubation time
Co-Authors A Heru Prianto AB SUSANTO Agung Suprihadi Ahmad Qi Sahlan Aninditia Sabdaningsih Aniza Rachmawati Anto Budiharjo Antonius Sarjiya Arina Aisyah Arina Tri Lunggani Barri Pratama Betalini Hapsari Bintoro Rudi Saputro Budi Raharjo Choirul Rizka Putri Dani Ika Purwaningsih Darnia Astari Parastiti Desi Arisanti Deverina Mergypta Devi Ayu Ningsih Devia Kusmawati Arfina Doni Usman Dwi Kristyaningrum Dwina Mulyaningtyas Dyah Palupi Endah Dwi Hastuti Erma Prihastanti Fauziah Citra Rahmawati Hadi Endrawati Hermin Hermin Hermin Pancasakti Kusumaningrum Indah Sulistyarini JOEDORO SOEDARSONO Khrisna Lazuardi Budi Laila Nur Faizah Lasria Pardede Linda Safitri Luthfy AN M Eka Prastya Mangasa Tua Pandapotan Siregar MG Isworo Rukmi Mia Tri Wardani Muhamad Fikri Zulfikar Muhammad Zaenuri Nabila Swarna Puspa Hermana Neny Nuraini Octavia Dewi Christiningrum Olivia Nisa Mumtianah Rejeki Siti Ferniah Risky Panji Nugroho Rully Rahadian Sari, Anisa Rachma Setiawan Wicaksono Sigit Hananto Siti Nur Jannah Sofyan Fauzi Larosa Sona Fatimah Soni Nugraha Anwar Sri Pujiyanto Susiana Purwantisari Syawal Nurangga Kodhatin T. A. Lestari Tri Muji Ermayanti Triwibowo Yuwono Vivi Suryaningsih w wijanarka Wijanarka Wijanarka Yadi Suryadi Yeti Darmayati Yoni Anggun Endah Kurniati