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All Journal JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Akademika Biologi Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Media Farmasi Indonesia
Endang Kusdiyantini
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Medicine & Pharmacology Other

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PRODUKSI SELULASE OLEH KAPANG Aspergillus sp. HASIL ISOLASI DARI LIMBAH PENGOLAHAN SAGU (Metroxylon sp.) DENGAN VARIASI KONSENTRASI INOKULUM PADA FERMENTASI TERENDAM STATIS Anisa Rachma Sari; Endang Kusdiyantini; MG Isworo Rukmi
Jurnal Akademika Biologi Vol. 6 No. 1 Januari 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Sago processing waste is one of agro-industry waste, which contains cellulose 19,55% and lignin 25%. Genus Aspergillus has been widely known as cellulase-producing fungi. Isolation of Aspergillus from sago processing waste is expected to get a potential isolates indegenous cellulolytic fungi. Cellulase (EC 3.2.1.4) is an enzyme that can break down cellulose into glucose monomer form, with consists of three components that work in synergy are endoglucanase, eksoglucanase and β-glucoside. This study aims to obtain isolates of Aspergillus sp. cellulolitic potential from sago processing waste and cellulase production of the cellulolytic isolates in various concentrations of inoculum. The study was conducted using completely randomized design (CRD) 1 factor of 4 levels of inoculum concentration treatments were 0%, 2,5%; 5%; and 10% with three replications. The observed variables are biomass, CMCase activity and FPase activity. Data were analyzed by ANOVA with a significance level 95%. The result of isolation, two isolates of Aspergillus namely A. flavus and A. paradoxus with cellulolitic index respectively 2,63 and 2,06. Cellulase activity isolate of A. flavus either endoglucanase (CMCase) or total cellulase (FPase) are not affected by the concentration of inoculum.Keywords: cellulase, sago waste, Aspergillus, inoculum
Pertumbuhan Tunas Tacca leontopetaloides L. Hasil Mikropropagasi Setelah Pemberian Radiasi Sinar Gamma Co60 dan Hormon Tumbuh yang Berbeda Darnia Astari Parastiti; Endang Kusdiyantini; Endah Dwi Hastuti; Betalini Hapsari; Tri Muji Ermayanti
Jurnal Akademika Biologi Vol. 4 No. 4 Oktober 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Taca  (Tacca  leontopetaloides  L.)  is  one  of  the  plant  family Dioscoreaceae  that  contain compounds   glikosida   flavonoid   called   taccalin   and   taccalonoides   who   potential   as antioxidants. Taka (Tacca  leontopetaloides L.) specifically has not been cultivated, grown only a limited area around the beach, it is necessary to plant vegetative propagation system faster with more results in tissue culture systems. This study aims to determine the growth and the antioxidant potential shoots Tacca leontopetaloides L. mikropropagasi results after administration  of  Co60  Gamma  ray  radiation  and  different  growth  hormones.  Research method  uses  completely  randomized  factorial  design  (RAL)  with  4  treatment  doses  of Gamma radiation Co60 is 0 Gy, 5 Gy, 20 Gy, and 30 Gy and 3 treatment of growth hormone that MSo, MSo + 0,5 BAP and MSo + 0,5 Kinetin with 3 replicates. Shoots taka from results mikropropagasi grown in  vitro and has been irradiated with Co60 Gamma rays. Parameters tested were growth of shoots, amount of leaves, till, roots and wet weight taka. The results showed  that  Gamma radiation  Co60  and  plant  regulator  cytokinin  effect  on  shoots  taka, amount of tillers, leaf, root and wet weight taka. Keywords: Taka (Tacca leontopetaloides L.), Mikropropagation, Growth Hormone.
Produksi dan Kestabilan Pigmen Merah Kapang Monascus sp. Menggunakan Media Tepung Kulit Singkong dengan Penambahan Bekatul pada Konsentrasi yang Berbeda Sona Fatimah; Agung Suprihadi; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The used of synthetic dyes have a various of negative effects on human health. Pigment produced by Monascus sp. can be used as an alternative natural coloring food. The purpose of this research is to produce pigment Monascus sp. using the substrate cassava peels flour with the addition of rice bran at various concentrations. The study was conducted by growing Monascus sp. on medium cassava peels flour with the addition of rice  bran in various concentrations (0%, 2.5%, 5% and 10%). Measured parameter is the production of red pigment and pigment stability against temperature, water content, solubility and pH. Red pigment production was measured using a spectrophotometer with a wavelength of 500 nm. Analysis of the data using One Way ANOVA and Duncan test. The results showed that the highest production of red pigment (5.6 CVU / gds) and water content of 47% was obtained on medium cassava peels flour with rice bran addition of 10%. Pigment stability towards heat are 0,39 at 300C, 0,35 at 600C, 0,27 at 1210C, and 0,22 at 1500C , stability pH 3 are 0,37 (0 hours incubation) dan 0,26 (12 hours incubation) whereas pH 7 are 0,38 (0 hours incubation) dan 0,36 (12 hours incubation).Keywords: Fermentation,Cassava peels flour, Rice bran, Monascus, Pigment intensity, stability
Produksi Pigmen Karotenoid oleh Khamir Phaffia rhodozyma yang Diperlakukan dengan Radiasi Sinar UV Sri Pujiyanto; Wijanarka Wijanarka; Endang Kusdiyantini; T. A. Lestari
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 2 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.2.146

Abstract

Carotenoid pigment is an essential element in aquaculture, since it gives characteristic of color on shrimp and fish. Carotenoid pigments can be produced microbiologically using Paffia rhodozyma. Genetic improvement of the yeast, one of which can be accomplished by radiation mutation, will increase the production of carotenoid pigments. The aims of this study were to mutate P. rhodozyma using UV irradiation and to figure out pigment production by the mutant strains resulting from 30 minute-irradiation. Irradiated culture was incubated in dark condition and plated onto YMA media. Grown mutant colonies were collected in order to test for their pigment production. Pigment production was measured on the basis of extinction coefficient of 1%. The results showed that mutant strain encoded with MUV-1 produced the highest pigment at 179.96 mg/g dry weight cell, higher than the wild type (63.20 mg/g dry weight cell).
Optimasi Starter dalam Memproduksi Inulinase dan Identifikasi Khamir Inulinolitik BAN - 1 dari Umbi Dahlia (Dahlia variabilis Willd) Wijanarka Wijanarka; Endang Kusdiyantini; Hermin Hermin
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 3 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.3.205

Abstract

Inulinolitic yeast isolated from dahlia tuber (Dahlia variabilis Willd) of Bandungan-Ambarawa, was found to produce inulinase enzyme (E.C.3.2.1.7).  Under tested fermentation condition, BAN-1 inulinase isolate showed enzymatic activity in dose dependent manner to the starter concentration. A maximum enzymatic activity of 0.582 IU was produced by adding starter concentration 10%. Based on morphological, physiological and biochamichal evaluation it was indentified that  the BAN-1 isolate is Pichia sp.
KARAKTERISASI MORFOLOGI SEL REKOMBINAN DAN PIGMEN HASIL FUSI PROTOPLAS INTERSPESIFIK Phaffia rhodozyma DAN Chlorella pyrenoidosaH Chick Indah Sulistyarini; Hermin Pancasakti Kusumaningrum; Endang Kusdiyantini
Media Farmasi Indonesia Vol. 8 No. 2 (2013): Media Farmasi Indonesia
Publisher : SEKOLAH TINGGI ILMU FARMASI YAYASAN PHARMASI SEMARANG

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Abstract

Protoplast fusion technique is a process offusion between protoplasts of theorganism to other organisms, so that the resulting recombinant cells with varying character derived from its parent. This study did Phaffia rhodozyma interspesies fusion and Chlorella pyrenoidosa. The purpose of this study was to analyze the morphological characteristics of cells results protoplast fusion recombinant yeast P. rhodozyma and microalgae C. pyrenoidosa. Protoplast fusion technique performed in 4 phases, namely the isolation of protoplasts, protoplast fusion, and analysis of recombinant cell morphological characters. The results showed that the recombinant cells have morphological characters derived from the parent P. rhodozyma and of the parent C. pyrenoidosa. But was character of the parent P. rhodozyma more dominant. Recombinant cells capable of producing pigment and lutein astaksanthin. Astaksanthin pigment levels in recombinant cell height in a shorter time than the parent P. rodozyma.
Co-Authors A Heru Prianto AB SUSANTO Agung Suprihadi Ahmad Qi Sahlan Aninditia Sabdaningsih Aniza Rachmawati Anto Budiharjo Antonius Sarjiya Arina Aisyah Arina Tri Lunggani Barri Pratama Betalini Hapsari Bintoro Rudi Saputro Budi Raharjo Choirul Rizka Putri Dani Ika Purwaningsih Darnia Astari Parastiti Desi Arisanti Deverina Mergypta Devi Ayu Ningsih Devia Kusmawati Arfina Doni Usman Dwi Kristyaningrum Dwina Mulyaningtyas Dyah Palupi Endah Dwi Hastuti Erma Prihastanti Fauziah Citra Rahmawati Hadi Endrawati Hermin Hermin Hermin Pancasakti Kusumaningrum Indah Sulistyarini JOEDORO SOEDARSONO Khrisna Lazuardi Budi Laila Nur Faizah Lasria Pardede Linda Safitri Luthfy AN M Eka Prastya Mangasa Tua Pandapotan Siregar MG Isworo Rukmi Mia Tri Wardani Muhamad Fikri Zulfikar Muhammad Zaenuri Nabila Swarna Puspa Hermana Neny Nuraini Octavia Dewi Christiningrum Olivia Nisa Mumtianah Rejeki Siti Ferniah Risky Panji Nugroho Rully Rahadian Sari, Anisa Rachma Setiawan Wicaksono Sigit Hananto Siti Nur Jannah Sofyan Fauzi Larosa Sona Fatimah Soni Nugraha Anwar Sri Pujiyanto Susiana Purwantisari Syawal Nurangga Kodhatin T. A. Lestari Tri Muji Ermayanti Triwibowo Yuwono Vivi Suryaningsih w wijanarka Wijanarka Wijanarka Yadi Suryadi Yeti Darmayati Yoni Anggun Endah Kurniati