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All Journal JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Akademika Biologi Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Media Farmasi Indonesia
Endang Kusdiyantini
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Medicine & Pharmacology Other

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ISOLASI, KARAKTERISASI BAKTERI ASAM LAKTAT, DAN ANALISIS PROKSIMAT DARI PANGAN FERMENTASI “TEMPOYAK” Arina Aisyah; Endang Kusdiyantini; Agung Suprihadi
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Tempoyak is a food made from fermented durian flesh with the addition of salt and brooded for seven days. Tempoyak quality is strongly influenced by the presence of microbes involved during the process of fermentation. This study aimed to isolate and characterize lactic acid bacteria (LAB) that play a role in the tempoyak fermentation process, and to perform proximate analysis of the durian and tempoyak. Isolation was done on 0, 3rd, 5th, and 7th day fermentation with streak methods. The isolates then underwent morphology and motility observation, biochemical tests, and proximate analysis.  The isolation of tempoyak resulted seven isolates of bacteria that had different colony and cell morphology. Gram staining of the bacterial cell produced a purple color with the rod and spherical shape. Motility test resulted non motile bacteria. Catalase test of bacteria isolates produced negative catalase. Isolates showed positive results in test for acid production and carbohydrate fermentation. pH decrease from 6.88 to 5.74 on the last day of brooding. Seven isolates obtained had characteristics that were similar with the characteristic of LAB, which was Gram positive, rod shaped or spherical, non motile, negative catalase, and produce acid. Tempoyak nutrient contained, ie 15.12% moisture content, 27.03% ash content, 2.69% fat content, 6.37% protein content, and 48.79% carbohydrate content. Keywords: tempoyak, LAB, morphology, biochemistry, proximate
ISOLASI DAN KARAKTERISASI ISOLAT KONSORSIUM BAKTERI LAHAN PERTANIAN SEBAGAI POTENSI DEGRADASI PESTISIDA PROPOXUR Ahmad Qi Sahlan; Endang Kusdiyantini; Sri pujiyanto
Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Propoxur pesticide is a pesticide used widely enough by the Indonesian agricultural community. This pesticide is used as a systemic pesticide in agricultural areas to deal with pests as well as insecticides, herbicides, and bactericide. Study of the degradation of propoxur has long performed using the bacterial bioremediation agent. Bacterial consortium is a combination of several bacteria in a medium which that has a complementary function in the environment. The purpose of this study was the isolation and characterization of a consortium of farms that use propoxur pesticide. Isolation was done by spread plate and was observed characteristics of colony morphology. The obtained isolates were tested against propoxur and its potential role in the consortium. Degradation of propoxur observations using High Performance Liquid Cromatography (HPLC). Isolation results obtained 8 (eight) bacterial isolates and one that have an important role in the consortium is K1 isolate. K1 isolate were able to work up a consortium to degrade Propoxur in 2 isolates with K2 isolate. The interaction of these bacteria are natural and have a tendency to vary in nature after a process of isolation. K1 isolate are not able to work alone in a mineral medium, and worked with synergistic and complementary that in the bacterial consortium. KSR consortium optimum growth occurs in an environment with temperature 35 °C and pH 6.Keywords: Isolation, Propoxur, Bioremediation, Bacterial Consortium, HPLC
PENGARUH KOMPOSISI MEDIUM PERENDAM TERHADAP MASA KESEGARAN BUNGA POTONG KRISAN (Chrysanthemum morifolium R.) Desi Arisanti; Erma Prihastanti; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 2 No. 4 Oktober 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Chrysanthemum morifolium is a cut flower with high economic value, however this flower has relatively short freshness period, thus it is necessary to use soaking solution by adding shredded basil leaves extract solution and carrageenan hydrogel material in it. Hydrogel serves as the water provider and energy substrate for respiration process of chrysanthemum. The basil leaves extract works as anti-bacterial and anti-fungal. The soaking is done to extend the freshness period of the chrysanthemums. This study aims to describe the effect ofthe composition of basil leaves extract and carrageenan hydrogel material on soaking medium to the freshness period of chrysanthemum, also to study the microorganism growth on the soaking medium. This research uses Completely Randomize Design on factorial pattern with two factors. First, Hydrogel Concentration (H): without hydrogel, 500 ml of water (H0); 10 g of carrageenan hydrogel, 400 ml of basil solvent (H1); 10 g of carrageenan hydrogel, 500 ml of basil solvent (H2); 10 g of carrageenan hydrogel, 600 ml of basil solvent (H3). Second, basil leaves extract (K): without basil leaves extract, without hydrogel (K0); 125 g/l of basil leaves extract (K1); 250 g/l of basil leaves extract (K2); 375 g/l of basil leaves extract (K3). This research uses 16 treatments 3 replications. The analysis is done by applying ANOVA, followed by Duncan’s test at the significance level of 95%. The research parameter includes the blooming flowers percentage, freshness period of spray chrysanthemum, and the condition of the soaking medium. The findings show that the composition of 400 ml of  250 g/l concentration of basil leaves extract, and 10 g of carrageenan hydrogel can extend the freshness period of chrysanthemum by 12.67 days, and prove that some microorganisms grow in the soaking medium. Keywords: spray chrysanthemums, soaking medium, basil leaves extract, carrageenan hydrogel, freshness period.
IDENTIFIKASI JENIS PIGMEN DAN UJI POTENSI ANTIOKSIDAN EKSTRAK PIGMEN BAKTERI Rhodococcus sp HASIL ISOLASI DARI SEDIMEN SUMBER AIR PANAS GEDONG SONGO Muhamad Fikri Zulfikar; Endang Kusdiyantini; Siti Nur Jannah
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Pigments are the coloring agents commonly used in the pharmaceutical, cosmetic, and food industries. This compound has the ability as antibacterial, anticancer, antibiotic and antioxidant. Pigment can be obtained synthetic and natural, natural pigments can be produced by plants, animals and microbes, one of which microbes is Rhodococcus sp. This study was conduct to identify the pigment found in Rhodococcus sp and measure the antioxidant activity of pigment produced by Rhodococcus sp. Growth and pigments production Rhodococcus sp was  grown on NB (Nutrient broth) medium with 96 hours incubation. This Growth was measured by dry weight cell. Identification of pigment using Thin Layer Chromatography and spectrophotometer UV-Vis. Measured of antioxidant activity Rhodococcus sp pigment using Carotene Bleaching methods. The results of pigments obtained from the identification by separation of pigment samples by thin layer chromatography resulted in 2 fractions on silica gel plate GF 250 with Rf values of 0.75 and 0.90. and analysis of UV-Vis spectrophotometers obtained optimum wavelengths 395 and 420 nm is beta-carotene pigments. Rhodococcus sp pigment measured its antioxidant activity with carotene bleaching method with the antioxidant activity value 51,24%.Keywords: Pigment, Rhodococcus sp, Pigment identification, antioxidant activity
Bioprospeksi Bakteri Yang Berasosiasi Dengan LamunEnhalus acoroidesdan Syringodium isoetifoliumSebagai Penghasil Senyawa Antibakteri Luthfy AN; Endang Kusdiyantini; Anto Budiharjo
Jurnal Akademika Biologi Vol. 4 No. 4 Oktober 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Bacteria can grow in variety of environments including those associated with marine organisms such as seagrasses, sponge, algae and soft corals. Bacteria association has the ability to produce bioactive compounds that can be used as a defense against pathogens and their hosts by producing secondary metabolites such as antibacteria compounds. This study aimed to asses the potential of bacteria associated with seagrasses Enhalus acoroides and Syringodium isoetifolium. This study was conducted with bacterial isolation from seagrass E. acoroides and S. isoetifolium, characterization of bacterial isolatesin morphology, antibacterial test, and biochemcal activity test. The result obtained four bacterial isolates, two isolates of Syringodium isoetifolium and two isolates of Enhalus acoroides. Two isolates from Syringodium isoetifolium had the largest ability inhibitory zone of inhibtion against the bacteria P. Aeruginosa (5.7mm) and against the bacteria E. coli (6.65mm).Key word : Antibacterial Compound, Seagrass-Associated Bacteria, Vibrio, Enhalus acoroides,      Syringodium isoetifolium
ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT PADA PANGAN FERMENTASI MANDAI Mangasa Tua Pandapotan Siregar; Endang Kusdiyantini; MG Isworo Rukmi
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

East Kalimantan has a lot of processed food products traditionally, one of them is mandai which is fermented food made from fruit leather Cempedak (Arthocarphus champeden). Lactic acid bacteria involved in the fermentation mandai greatly affect the quality of the final product. This study aimed to perform the isolation and characterization of lactic acid bacteria in the mandai fermentation process. MRS medium was used in the isolation of bacteria for 14 days. Isolation of bacteria during the fermentation process mandai obtaining 17 isolates, nine isolates Gram negative isolates, and eight isolates Gram positive. Eight isolates showed positive results against some of the morphological and biochemical characterization. Including biochemical characterization, acid formation and gas production from glucose, motility test, catalase test, growth at 160C and 480C, hydrolysis of starch, fat hydrolysis, and hydrolysis of casein and acid formation test. Eight isolates bacteria made mandai into processed food products that had a sour taste. Keywords: lactic acid bacteria, BAL ,bacterial isolates, cempedak skin.
EKSPLORASI DAN KARAKTERISASI BAKTERI POTENSIAL PENGHASIL SENYAWA ANTIFOULING YANG BERASOSIASI DENGAN ALGA COKLAT (PHAEOPHYTA) DI PERAIRAN KEPULAUAN KARIMUNJAWA JEPARA Barri Pratama; Endang Kusdiyantini; Agung Suprihadi; Anto Budiharjo; AB Susanto
Jurnal Akademika Biologi Vol. 3 No. 4 Oktober 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Biofouling is a settlement process from marine organisms initiated by biofilm (microbial layers). Corrosion on the vessels, pipelines under the sea, oil plants, etc are big problems in the industrial today cause adversely affects. Antifouling paints have been developed to prevent settlement of organisms, however antifouling paints contain heavy metals and biocides which have toxic effects on marine ecosystems. Antifouling compound which environmental friendly is a solution for one of the paint component. The aim of this research was to isolate bacteria associated with brown algae which had potential to produce antifouling compound from Karimunjawa islands, Jepara.Isolate KS1-1 showed antifouling activity against Pseudomonas aeruginosa as biofilm-forming bacteria.This isolate had been characterized in morphology, moleculer and biochemichal identification.Moleculer analyses of 16S rRNA sequence, KS1-1 showed similarity 94% from 287 bp with Vibrio sp. W-137-16S ribosomal RNA gene, partial sequence. Keywords : Antifouling, Phaeophyta,Biofouling, 16S rRNA, Vibrio
KERAGAMAN GENETIK JAHE (Zingiber officinale Roscoe) MENGGUNAKAN TEKNIK PENANDA MOLEKULER Random Amplified Polymorphic DNA (RAPD) Choirul Rizka Putri; Hermin Pancasakti Kusumaningrum; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 5 No. 2 April 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Ginger plant in Indonesia is known there are three kinds of varieties, namely red ginger, emprit ginger and big ginger. Ginger (Zingiber officinale Roscoe) is one of the medicinal plants that could potentially be produced in increasing quantities and wide distribution in Indonesia that may affect patterns of genetic diversity. This study aims to determine patterns of genetic diversity of three varieties of ginger (red ginger, big ginger and emprit ginger) obtained from BALITRO, Bogor and BPTP, Ungaran using two primers (OPA1 and OPA2). Techniques of molecular markers RAPD (Random Amplified Polymorphic DNA) is one of the techniques used for the analysis of genetic diversity. The study begins by isolating DNA from 30 samples of ginger leaves, then amplification with primers OPA1 and OPA2 which then results of electrophoresis converted into the form of binary data and phylogenetic tree construction with 2:02 NYTSYS program. The results obtained are worth a total of 20 polymorphic band (86.9%) and 3 pita monomorphic (13%) with primary OPA1 and OPA2. Heterozygosity values in a row is worth 0.9026 and 0.889. Distance genetic similarities in this sample has the highest value is 1 and the lowest is 0.3. The relationship between the three varieties of big ginger are distant relationship with red ginger and emprit ginger. Red ginger and emprit ginger still have a close genetic relationship. Key Word               : Genetic Diversity, Zingiber officinale, RAPD
IDENTIFIKASI SENYAWA BIOAKTIF PADA ISOLAT BAKTERI BUAH BELIMBING WULUH (Averrhoa bilimbi L.) SEBAGAI AGENSIA HAYATI Xanthomonas oryzae pv. oryzae Aniza Rachmawati; Agung Suprihadi; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Bilimbi (Averrhoa bilimbi L.) is one of original fruit plants from Indonesia. Bilimbi fruits contain flavonoid, saponin, triterpenoid and tanin that have ability as anti-microbial. Bacteria was isolated from bilimbi fruits expected to be able produce bioactive compounds which can kill pathogenic bacteria such as Xanthomonas oryzae pv. oryzae which caused Bacterial Leaf Blight (BLB). Bacterial Leaf Blight in Indonesia caused harvest losses of 18 – 36 %. Infection was caused by X. oryzae caused the leaf symptoms to turn pale yellow, white, withered, and finally die. The purpose of this research are get bacteria isolate from bilimbi fruits (Averrhoa bilimbi L.) and testing bioactive compounds on bacteria isolate from bilimbi fruits which can inhibit Xanthomonas oryzae pv. oryzae (Xoo) growth. This research use methods, that are isolation of bacteria from bilimbi fruits and isolation of Xanthomonas oryzae pv. oryzae, characterization biochemically, inhibition test, and Thin Layer Chromatography. Obtained two bacteria isolate from bilimbi fruits. They are IBW1 dan IBW2, inhibition zona of IBW1 is 0,15 mm and IBW2 0,35 mm. Both of them have potential in antibacteria of X. oryzae bacteria eventhough in weak catagory. Metabolite secondary compound which play a role in antibacteria of X. oryzae is flavonoid compound. Kata kunci: antibacteria, bilimbi, Thin Layer Chromatography, X. oryzae
EKSPLORASI RHIZOBAKTERI INDIGENOUS TANAMAN CABAI RAWIT (Capsicum frustescens Linn.) DARI PERTANIAN SEMI ORGANIK DESA BATUR KABUPATEN SEMARANG SEBAGAI AGEN HAYATI PENGENDALI PERTUMBUHAN JAMUR Fusarium oxysporum f.sp capsici M Eka Prastya; Agung Suprihadi; Endang Kusdiyantini
Jurnal Akademika Biologi Vol. 3 No. 3 Juli 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Rhizobacteria is a group of bacteria that live in the around of plant roots. This type of bacteria known have the ability to stimulate plant growth by producing growth hormone, as well as to inhibit the growth of plant pathogens synthesize compounds with antibiotics or extracellular enzymes. The purpose of this study was to obtain and describe the morphological, biochemical and genetic isolates rhizobacteria of semi-organic farmland Semarang District village Batur which has the ability as a biological control agent of fungal pathogens Fusarium oxysporum f.sp capsici. The results obtained fifteen isolates the majority rhizobacteria bacilus shaped gram-positive and classified. Rhizobacteria inhibition test capabilities against pathogenic fungi was performed using dual culture test and test biomass. Dual culture test results showed that the inhibition of isolates E1 has a 3.77%, 1.88% isolates E3 and E15 isolates 22%. The biomass tests show E15 isolates capable of inhibiting the growth of pathogenic fungi best with the smallest weight of fungal biomass 0.0386 grams. The results of the molecular characterization based on 16S rRNA gene sequences to known that E15 isolates has similar with Bacillus cereus strain ATCC 14579 with similarity of 97%. The results of the biochemical characterization of isolates E15 has similarities with B. cereus species that is catalase positive, motile, have endospores, is able to hydrolyze starch and ferment glucose.Keywords: rhizobacteria, Fusarium oxysporum f.sp capsici, gen 16S rRNA, Bacillus cereus
Co-Authors A Heru Prianto AB SUSANTO Agung Suprihadi Ahmad Qi Sahlan Aninditia Sabdaningsih Aniza Rachmawati Anto Budiharjo Antonius Sarjiya Arina Aisyah Arina Tri Lunggani Barri Pratama Betalini Hapsari Bintoro Rudi Saputro Budi Raharjo Choirul Rizka Putri Dani Ika Purwaningsih Darnia Astari Parastiti Desi Arisanti Deverina Mergypta Devi Ayu Ningsih Devia Kusmawati Arfina Doni Usman Dwi Kristyaningrum Dwina Mulyaningtyas Dyah Palupi Endah Dwi Hastuti Erma Prihastanti Fauziah Citra Rahmawati Hadi Endrawati Hermin Hermin Hermin Pancasakti Kusumaningrum Indah Sulistyarini JOEDORO SOEDARSONO Khrisna Lazuardi Budi Laila Nur Faizah Lasria Pardede Linda Safitri Luthfy AN M Eka Prastya Mangasa Tua Pandapotan Siregar MG Isworo Rukmi Mia Tri Wardani Muhamad Fikri Zulfikar Muhammad Zaenuri Nabila Swarna Puspa Hermana Neny Nuraini Octavia Dewi Christiningrum Olivia Nisa Mumtianah Rejeki Siti Ferniah Risky Panji Nugroho Rully Rahadian Sari, Anisa Rachma Setiawan Wicaksono Sigit Hananto Siti Nur Jannah Sofyan Fauzi Larosa Sona Fatimah Soni Nugraha Anwar Sri Pujiyanto Susiana Purwantisari Syawal Nurangga Kodhatin T. A. Lestari Tri Muji Ermayanti Triwibowo Yuwono Vivi Suryaningsih w wijanarka Wijanarka Wijanarka Yadi Suryadi Yeti Darmayati Yoni Anggun Endah Kurniati