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All Journal Jurnal Ilmiah Farmasi Jurnal Ilmu Dasar e-Journal Pustaka Kesehatan Jurnal Kimia Terapan Indonesia JSFK (Jurnal Sains Farmasi & Klinis) Jurnal Jamu Indonesia Jurnal Tumbuhan Obat Indonesia INSAN CENDEKIA : Jurnal Pengabdian Kepada Masyarakat Jurnal Kefarmasian Indonesia Journal of Agropharmacy Indonesian Journal of Jamu
Lestyo Wulandari
Fakultas Farmasi Universitas Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Economics, Econometrics & Finance Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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Journal : Journal of Agropharmacy

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Penentuan Kadar Flavonoid Total dan Klasifikasi Serbuk Daun Murbei (Morus alba L.) Berbeda Ketinggian Tempat Tanam Syaifullah, Abim; Kristiningrum, Nia; Pangaribowo, Dian Agung; Wulandari, Lestyo
Journal of Agropharmacy Vol. 1 No. 1 (2024)
Publisher : Faculty of Pharmacy, University of Jember

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Abstract

Mulberry (Morus alba L.) is a plant from the Moraceae family which has a high flavonoid content. This research aims to determine the ability of NIR spectroscopy and chemometrics to classify mulberry leaf powder based on different planting heights and determine the total flavonoid content using the Uv-Vis spectrophotometric method. The classification of mulberry leaf powder originating from the lowlands, midlands, and highlands provides valid results using NIR spectra and chemometric data from the LDA and SIMCA models with an accuracy value of 100%. The average results of determining total flavonoid content using Uv-Vis spectrophotometry showed that the highest levels were in the highlands, followed by the medium and lowlands with total flavonoid levels respectively, namely 6.453 ± 1.194 mg QE/g powder; 5.889 ± 0.944 mg QE/g powder; and 5.177 ± 0.998 mg QE/g powder. The results of the One Way ANOVA test showed that the total flavonoid content of mulberry leaf powder from different altitude areas did not have a significant difference.
Validasi Metode Penentuan Kadar Asam Klorogenat dan Kafein secara Simultan pada Serbuk Daun Kopi Robusta (Coffea Canephora) dengan Metode KLT Densitometri Putri Aditama, Tiara Sagita; Kristiningrum, Nia; Wulandari, Lestyo; Holidah, Diana; Alishlah, Tanfidz; Puspitasari, Endah
Journal of Agropharmacy Vol. 2 No. 1 (2025)
Publisher : Faculty of Pharmacy, University of Jember

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Coffee contains bioactive compounds such as chlorogenic acid and caffeine. Chlorogenic acid is recognized as a natural antioxidant, while caffeine is widely used to reduce fatigue and drowsiness. This study aims to simultaneously determine the content of chlorogenic acid and caffeine in robusta coffee leaf powder (Coffea canephora) using Thin Layer Chromatography (TLC) densitometry. The study identified an optimal eluent composition of ethyl acetate:formic acid:distilled water (18:1:1), with maximum wavelengths of 330 nm for chlorogenic acid and 274 nm for caffeine. High linearity was demonstrated with correlation coefficients of 0.993 for chlorogenic acid and 0.994 for caffeine. Sensitivity was validated with LOD values of 100.664 ng for chlorogenic acid and 297.232 ng for caffeine, and LOQ values of 301.994 ng for chlorogenic acid and 891.697 ng for caffeine. Selectivity was confirmed as the method effectively separated chlorogenic acid, caffeine, and other compounds with a resolution (Rs) ≥ 1.5, while specificity was evidenced through purity and identity values exceeding 0.99. Precision was proven through repeatability (%RSD for chlorogenic acid: 2.94%; caffeine: 2.27%) and intermediate precision (%RSD for chlorogenic acid: 2.26%; caffeine: 2.18%). Accuracy was achieved with mean recovery rates of 102.22% for chlorogenic acid and 99.73% for caffeine. The determination of chlorogenic acid and caffeine concentrations in robusta coffee leaf powder revealed variations between samples: PTPN XII Renteng yielded 2.278% ± 0.209% for chlorogenic acid and 1.171% ± 0.821% for caffeine, while Puslitkoka Indonesia samples showed 1.238% ± 0.206% for chlorogenic acid and 0.917% ± 0.823% for caffeine. Based on validation parameter assessments, the method is deemed valid as it meets all acceptance criteria for each parameter. This research provides an efficient and accurate analytical approach for simultaneous determination of bioactive compounds in robusta coffee leaves.
Co-Authors Amin, Muhammad Zainal Roisul Ani Mubayinah Ari Satia Nugraha Ari Satya Nugraha Arjun Nurfawaidi Arrofita Ani Sandiya Azhari, Nuri Putri Bambang Kuswandi Bambang Kuswandi Bambang Sri Kaloko Dewi Enggar Fitriani Dewi Kusumaningrum Pamungkas Dian Agung Pangaribowo Diana Holidah Diyanul Mustafidah Dwi Citra Nur Utami Endah Puspitasari Fadhila, Nadila Ikhda Ramadhani Fauzan Arrozi Fracilia Arinda Ratnasari Hilmia Lukman, Hilmia Himmah, Ulfa Aliyatul Irwin Ulil Hidayah Jazila, Yusrin Jazila, Yusrin Nur Lukmanto, Lukmanto Marizka Wimala Nia Kristiningrum Nugraha, Ari Satya Nuri Putri Azhari Putri Aditama, Tiara Sagita Reny Diastri Noviriana Syaifullah, Abim Tanfidz Alishlah, Tanfidz Ulfa Aliyatul Himmah Yuni Retnaningtyas