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The Effect of Carica Pubescens Lenne and K. Koch Fruit Extract from Dieng Plateau and Cangar to the Amount of Fibroblasts Cells on the Healing of Oral Mucosal Inflammation Kristanti, Risma Aprinda
Journal of Islamic Pharmacy Vol 1, No 1 (2016): J. Islamic Pharm.
Publisher : Universitas Islam Negeri Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/jip.v1i1.3457

The purposes of this research are to know the effect of C.pubescens fruit extract on the amount of gingival fibroblasts in wound healing of Rattus norvegicus mouth cavityâ€™s mucosal and to know the effect of C.pubescens comes from two different areas (Dieng and Cangar) on the amount of gingival fibroblasts in wound healing of Rattus norvegicus oral mucosa. Twenty eight rats are divided to be four groups (K1, K2, K3, and K4), each rat is wounded 1 cm on the gingival mucosa of lower jaw (specifically on the apical region of incisive teeth). K1 is the control group with aquadest treatment on the wound. The wound in the K2 is treated with C.pubescens fruit extract from Dieng. The wound in the K3 is treated with C.pubescens fruit extract from Cangar. And the treatment for K4 is medicated by policresulen (common medicine for oral mucosal wound). On the fifth day of the treatment, all rats are sacrificed, and the gingival tissue is taken up for the next step. Gingival tissue is smeared by Haematoxylin Eosin (HE) to analyze the amount of gingival fibroblasts histologically. The result of this research shows that the highest average amount of gingival fibroblasts comes from K4 (policresulen treatment). And there is no significant difference on the number of Rattus norvegicus gingival fibroblasts from all of the groups (K1, K2, K3, and K4).Â Keywords: fibroblast, gingival, wound, mucosa, mouth, C.pubescens

PENGARUH PEMBERIAN EKSTRAK DAUN CARICA PUBESCENS Lenne & K. koch TERHADAP KETEBALAN EPITEL GINGIVA MENCIT BETINA YANG DIPAPAR ASAP ROKOK Aprinda Kristanti, Risma
Journal of Islamic Medicine Vol 1, No 1 (2017): Journal of Islamic Medicine
Publisher : Faculty of Medicine and Health Science, Universitas Islam Negeri Maulana Malik Ibrahim

The exposure from cigarette smoke in long term can destroy oral mucous, thickening of the epithel gingival tissue, and keratotic white spot that sign leukoplakia and oral cancer. Several components in the C.pubescens leaf for example atsiri oil, polifenol, and flavonoid are kown has antioxidant acivity. The major component in C.pubescens leaf that have antioxidant activity is vitamin C. The aim of this study is to examine the effect of C. pubescens leaf extract to the thickness of gingival tissue from female mices that got cigarette smoke exposure. Twenty five adult female mices were devided into five group and they exposed by cigarette smoke for twenty one day. K1 group as negative control were given by cmc, K2 group as positive control were given by 0,2 mg vitamin C, K3 group were given by 0,5 g/KgBW C. pubescens extract, K4 group were given by 1 g/KgBW C. pubescens extract, and K5 group were given by 2 g/KgBW C. pubescens extract. At the end of 22nd day, the mices were sacrificed and the gingival mucosa were collected for histologist preparations.The result of this study shows that K2 group has the thickest gingival epithel layers while there is significant different among five group for the thikness gingival epithel layers.

FRUCTOSE ALTERS THE EXPRESSION OF TYPE B LEPTIN RECEPTOR IN HYPHOTALAMUS AND INTESTINUM OF Rattus Novergicus Rachmawati, Ermin; Kristanti, Risma Aprinda; Susanti, Nurlaili
Journal of Islamic Medicine Vol 2, No 2 (2018): Journal of Islamic Medicine
Publisher : Faculty of Medicine and Health Science, Universitas Islam Negeri Maulana Malik Ibrahim

Background: Several epidemiological dataâ€™s reported significant correlation between fructose consumption and diabetes mellitus type 2 by inducing insulin resistance. Leptin resistance induced by fructose was proposed as one novel mechanism that induce insulin resistance but the exact mechanism remains unclear. Â We hypothesize that fructose diminish the type b leptin receptors in hypothalamus and intestine.Aim: This study was aimed to elucidate fructose effect on the expression of leptin receptor type b in hypothalamus and intestine of Rattus novergicus.Method: twenty eight rats were used and divided into 4 groups: Group 1 was control, group 2 was given fructose 10%, group 3 was given fructose 30% and group 4 was given fructose 55% for 2 months. At the end of treatment, the animal were sacrificed and then the hypothalamus and intestine were collected. The expression of type b leptin receptor were measured by immunohistochemistry technique with primary antibody from Bioss antibodies type Leptin receptor polyclonal antibody bs-0109R using Staining kit Skytec Laboratories and DAB chromogen. A positive expression can be seen as a brown colour in cell cytoplasm and counted in 100 cell. The expression then analysed using SPSS 18 with anova one way tes (p0,05) followed by post hoc test after the data showed normality and homogeneity using Saphiro wilk and Levene test (p0,05).Result: There was significant differences in type b leptin receptors found in hypothalamus between each group (p0.05). The significant differences also could be seen in the expression between control and group fructose 30 and 50% in intestinal cell (p0.05).Conclusion: the consumption of Fructose 55% for 2 months attenuates the expression of type b leptin receptors in hypothalamus and intestine of Rat novergicus.Â

PENGARUH PEMBERIAN DIET TINGGI LEMAK TERHADAP KONSENTRASI SECRETORY IMMUNOGLOBULIN A (sIgA) SALIVA TIKUS PUTIH Rattus norvegicus YANG DIINDUKSI BAKTERI Aggregatibacter actinomycetemcomitans (Aa) Kristanti, Risma Aprinda
Journal of Islamic Medicine Vol 2, No 2 (2018): Journal of Islamic Medicine
Publisher : Faculty of Medicine and Health Science, Universitas Islam Negeri Maulana Malik Ibrahim

Hiperlipidemia menyebabkan perubahan pada sel imun dan proses penyembuhan luka, sehingga juga meningkatkan kemungkinan untuk terserang periodontitis yang terutama disebabkan oleh bakteri Aggregatibacter actinomycetemcomitans (Aa) atau infeksi lainnya. Asam lemak yang berlebihan dapat menghambat proliferasi sel karena hasil peroksidasi lipid yang bersifat toksik bagi sel. Asam lemak juga ikut mempengaruhi produksi dan modulasi sitokin karena asam lemak dapat menyebabkan reduksi proliferasi limfosit. Diet tinggi lemak telah menyebabkan penurunan jumlah limfosit B. Pada perkembangannya, limfosit B membentuk sel B memori dan sel plasma yang memproduksi antibodi, termasuk IgA. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian diet tinggi lemak dan induksi bakteri Aa terhadap konsentrasi secretory Immunoglobulin A (sIgA) saliva. Sebanyak 28 tikus Rattus norvegicus Strain Wistar dibagi dalam empat kelompok secara random, kelompok K0 diberi pakan standar, kelompok K1 diberi pakan standar kemudian diinduksi bakteri Aa, kelompok K2 diberikan diet tinggi lemak, dan kelompok K3 diberikan diet tinggi lemak kemudian diinduksi bakteri Aa. Pada akhir penelitian seluruh tikus diambil cairan saliva dan diukur konsentrasi sIgA saliva menggunakan metode ELISA. Dari hasil penelitian menunjukkan bahwa terdapat perbedaan yang signifikan konsentrasi sIgA saliva antara kelompok pakan standar tanpa induksi bakteri Aa (K0) dengan kelompok diet tinggi lemak tanpa induksi bakteri Aa (K2) dan antara kelompok pakan standar dengan induksi bakteri Aa (K1) dengan kelompok diet tinggi lemak dengan induksi bakteri Aa (K3)Â

PENGARUH OKSITOSIN TERHADAP KONTRAKSI OTOT POLOS UTERUS Kristanti, Risma Aprinda
El-Hayah : Jurnal Biologi Vol 5, No 1 (2014): EL-HAYAH (VOL 5, NO 1, September 2014)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v5i1.3036

Stimulus yang memicu sebagian besar kontraksi otot polos adalah adanya peningkatan ion kalsium intra sel. Peningkatan ini dapat ditimbulkan pada jenis otot polos yang berbeda oleh perangsangan saraf pada serabut otot polos, stimulasi hormon, regangan serabut, atau bahkan perubahan pada lingkungan kimiawi serabut. Suatu hormon dapat menimbulkan kontraksi otot polos bila membran sel otot mengandung reseptor perangsang bergerbang hormon untuk hormon tertentu. Beberapa reseptor hormon pada membran otot polos akan membuka kanal ion kalsium dan natrium serta menimbulkan depolarisasi membran. Kadang timbul potensial aksi, atau justru mungkin memperkuat potensial aksi yang telah terjadi. Pada keadaan lain, terjadi depolarisasi tanpa disertai dengan potensial aksi, dan depolarisasi ini membuat ion kalsium masuk ke dalam sel sehingga terjadi kontraksi. Oksitosin merangsang kontraksi uterus melalui mekanisme Ca2+ dependent dan Ca2+ independent. Pada jalur Ca2+ dependent, beberapa reseptor hormon pada membran otot polos akan membuka kanal ion kalsium dan natrium serta menimbulkan depolarisasi membran. Jalur Ca2+ independent adalah dengan melalui jalur Rho kinase. Rho yang telah teraktivasi meningkatkan fosforilasi rantai ringan miosin pada konsentrasi kalsium yang konstan, ini menunjukkan bahwa Rho memiliki peran pada mekanisme pengaturan â€œCa2+ sensitizationâ€

PENGARUH EKSTRAK BUAH Carica pubescens Lenne & K. Koch YANG TUMBUH DI BEBERAPA TEMPAT DI INDONESIA TERHADAP PENYEMBUHAN LUKA MUKOSA RONGGA MULUT Kristanti, Risma Aprinda
El-Hayah : Jurnal Biologi Vol 5, No 3 (2015): EL-HAYAH (VOL 5, NO 3,September 2015)
Publisher : Department of Biology Science and Technology Faculty UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v5i3.3099

The aim of this study is to examine the effect of Carica Â pubescens Lenne K. Koch extract to the epihtelialization on the wound healing of gingival mucosa and to examine the effect of C. pubescens extract from different region (Dieng and Cangar) to the epithelialization process.Twenty eight adult male albino rats were devided into four group, we created cutlet along 1 cm on the mandibular gingival mucosa below insisiv teeth. K1 group were treated with aquadest while K2 group were treated with C. pubescens extract from Dieng, K3 group were treated with C. pubescens extract from Cangar, and K4 group were treated with policresulen preparations. At the end of fifth day, the rats were sacrificed and the mandibular gingival mucosa were collected for histologist preparations. The result of this study shows that K3 group has the thickest gingival epithel layers while there is no significant different among four group for the thikness gingival epithel layers

PENGGUNAAN DOKSISIKLIN HYCLATE SEBAGAI INHIBITOR MATRIKS METALLOPROTEINASE PADA TERAPI TAMBAHAN PERIODONTITIS Risma Aprinda Kristanti
SAINSTIS SAINSTIS (Vol 1, No 2
Publisher : Lembaga Penelitian dan Pengembangan Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Terbit.com Daily has reported that the prevalence of periodontitis increases related with age person. Found that 35,7% of patients with periodontitis in the group 30-39 years old to 66,5% in the group 50-59 years old, increase 89,2% in the group 80-90 years old. Periodontitis that commonly found is a chronic periodontitis which is occurs in individuals older than 45 years, but can also be found in children. The main characteristic of periodontitis is the damage of the periodontal connective tissue, alveolar bone, and the movement of the junctional epithelium to the apical. Bacterial components may directly or indirectly cause tissue damage. Inflammatory mediators product such as proteinases, cytokines, and prostaglandins are part of the host response that can also cause tissue damage. Matrix metalloproteinase (MMP) is a proteinase that can cause periodontal tissue damage by disrupting the extracellular matrix molecules in periodontal tissues. Since is known that MMP has a role in various pathological processes, therapy to inhibit activity MMP in pathological processes has developed. MMP inhibitors will be a useful adjunct therapy for the treatment of periodontal disease. Many products have been introduced as an MMP inhibitor, for example: tetracycline and various derivatives, such as doxycycline and minocycline which are capable of inhibiting the activity of several classes of MMPs. Doxycycline has the ability to inhibit the increase in collagenase activity that is pathological in subgingival and prevent periodontitis to become more severe.

Synergistic Effects of Doxorubicin and Cardenolid Glycosides of Calotropis Gigantea Root on Cervical Cancer Hela Cell Line Roihatul Mutiah; Risma Aprinda Kristanti; Siti Maimunah
Majalah Obat Tradisional Vol 22, No 2 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Cardenolid glycosides (CGs) has been known has high anticancer activity against several types of cancers, for example, breast cancer, lung cancer, prostate cancer, melanoma, neuroblastoma, myeloma and leukemia in the in vitro and in vivo research. The aim of our study was to know the synergistic potency of CGs improving the efficacy of doxorubicin in cervical cancer. Activities from combination of doxorubicin and CGs was measured by MTT colorimetric methode. Combination Index was used as combination efficacy parameter. The results showed that the IC50 of CGs was 1,023 μg/mL. CGs demonstrated selective activity in inhibiting the growth of HeLa cells by selectivity index > 3 (241.9). Combination test for CGs and Doxorubicin showed strong synergistic effect in doses 200 ug/mL Doxorubicin and 1.79 ug/mL CGs. The synergistic effect has been shown by 10 combination doses for the doses below the IC50. CGs increases the efficacy of Doxorubicin for the doses below the IC50, thus CGs can be recommended as co chemotherapy in cervical cancer treatment.

Efek Diet Tinggi Fruktosa terhadap Profil Lipid Tikus Rattus Rattus norvegicus Strain Wistar Nurlaili Susanti; Ermin Rachmawati; Risma Aprinda Kristanti
Journal of Islamic Medicine Vol 3, No 1 (2019): Journal of Islamic Medicine
Publisher : Faculty of Medicine and Health Science, Universitas Islam Negeri Maulana Malik Ibrahim

Sejak beribu tahun yang lalu, orang mengonsumsi fruktosa dalam jumlah terbatas dari buah-buahan segar dan sayuran. Fruktosa yang terdapat dalam bahan alami ini tidak membahayakan kesehatan. Akan tetapi saat ini, fruktosa banyak digunakan sebagai pemanis oleh industri makanan dan minuman dalam bentuk high fructose corn syrup (HFCS). Fruktosa sangat efisien menginduksi lipogenesis. Penelitian ini dilakukan untuk membuktikan pengaruh konsumsi fruktosa terhadap profil lipid pada hewan coba. Penelitian ini merupakan penelitian eksperimental laboratoris dengan desain postest only controle group. Sampel dibagi dalam 4 kelompok perlakuan dengan masing-masing 6 ekor tikus terdiri dari tikus diet normal (K), tikus diet fruktosa 10% (P1), tikus diet fruktosa 30% (P2), dan tikus diet fruktosa 60% (P3). Fruktosa diberikan secara peroral dengan menggunakan sonde selama 8 minggu. Pada akhir penelitian, dilakukan pengambilan sampel darah untuk pengukuran profil lipid dengan spektrofotometer. Hasil penelitian pada tikus yang diberikan diet fruktosa menunjukkan kadar kolesterol total, LDL, dan trigliserida yang signifikan lebih tinggi dibandingkan kontrol, sedangkan kadar HDL lebih rendah dibandingkan kontrol. Dapat disimpulkan bahwa diet tinggi fruktosa dapat menginduksi terjadinya dislipidemia. Kata Kunci: fruktosa, profil lipid.

Compound Identification and Anticancer Activity of Ethyl Acetate Fraction from Bawang Sabrang (Eleutherine palmifolia (L.) Merr.) on HeLa Cervical Cancer Cell Line Mutiah, Roihatul; Minggarwati, Trian Sidha; Kristanti, Risma Aprinda; Susanti, Erna
Indonesian Journal of Cancer Chemoprevention Vol 10, No 3 (2019)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev10iss3pp131-139

Eleutherine palmifolia (L.) Merr. is a typical plant found in Central Kalimantan that has been used empirically by the Dayak people as medicine for various diseases, including cancer. The plant contains flavonoid compounds that potentially used as an anticancer. The purpose of this study is to find the most active fraction, indicated by its cytotoxic potency on HeLa cervical cancer cell line, and to identify compounds in E. palmifolia bulbs fraction. E. palmifolia bulbs was extracted by maceration. The extraction with ultrasonic bath and partition fractionation was conducted by using n-hexane, chloroform, and ethyl acetate. Each fraction was tested for toxicity level on HeLa cells using MTT assay. The identification of active compounds was carried out by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS). The result showed that based on the IC50 value, the ethyl acetate fraction had the highest bioactivity. IC50 values of n-hexane, chloroform, and ethyl acetate fractions were 250.77±19.01; 720.46±42.38; and 44.34±9.45μg/mL, respectively. The identification of the active compound in ethyl acetate fraction resulted 28 chemical compounds. Compounds with the highest percentage area were isoliquiritigenin and oxyresveratrol. The ethyl acetate fraction of E. palmifolia bulbs is potential to be developed as an anticancer candidate (phytopharmaceutical).Keywords: Compound identification, Anticancer activity, Eleutherine palmifolia (L.) Merr., cervical cancer

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