Rhesi Kristiana
Program Studi Budidaya Perairan, Jurusan Perikanan, Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

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PENGARUH PADAT TEBAR TINGGI TERHADAP KELANGSUNGAN HIDUP, KONSUMSI PAKAN DAN EFISIENSI PAKAN SERTA PERTUMBUHAN JUVENIL LOBSTER AIR TAWAR (Cherax sp.) Kristiana, Rhesi; Arini, Endang; Hastuti, Sri
Journal of Aquaculture Management and Technology Volume 3, Nomor 3, Tahun 2014
Publisher : Journal of Aquaculture Management and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.781 KB)

Abstract

Lobster air tawar (Cherax sp.) merupakan salah satu jenis lobster yang telah dibudidayakan di Indonesia. Padat penebaran merupakan salah satu faktor kunci untuk mencapai produksi yang optimal, terkait dengan budidaya intensif untuk memperoleh produksi yang tinggi. Penelitian ini bertujuan untuk mengkaji pengaruh padat tebar terhadap kelangsungan hidup, konsumsi pakan dan efisiensi pakan serta pertumbuhan lobster air tawar stadia juvenil (Cherax sp.). Penelitian ini dilaksanakan pada bulan Oktober – Desember 2013 di Laboratorium Basah Budidaya Perairan, Jurusan Perikanan, Fakultas Perikanan dan Ilmu Kelautan Universitas Diponegoro, Semarang. Hewan uji yang digunakan adalah Lobster Air Tawar (Cherax sp.) umur 40 hari. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 3 kali ulangan yaitu perlakuan A (padat penebaran 200 ekor/m2), B (padat penebaran 250 ekor/m2), C (padat penebaran 300 ekor/m2), D (padat penebaran 350 ekor/m2). Variabel yang diukur adalah glukosa darah, tingkat konsumsi pakan, efisiensi pemanfaatan pakan, pertumbuhan, kelulushidupan dan kualitas air. Hasil penelitian menunjukkan ada pengaruh (P<0,05) padat penebaran terhadap efisiensi pemanfaatan pakan, pertumbuhan dan kelulushidupan lobster air tawar. Perlakuan A (padat penebaran 200 ekor/m2)memiliki hasil tertinggi pada efisiensi pemanfaatan pakan yaitu 64,50±1,23%, pertumbuhan yaitu 1,81±0,02% dan kelulushidupan yaitu 90,28±2,41%. Sedangkan pada perlakuan D (padat penebaran 350 ekor/m2) memiliki hasil terendah yaitu efisiensi pemanfaatan pakan 41,80±0,56%, pertumbuhan 1,02±0,00% dan kelulushidupan 65,87±1,37%. Padat tebar yang terbaik untuk pertumbuhan dan kelulushidupan lobster air tawar stadia juvenil adalah 200 ekor/m2. Freshwater crayfish (Cherax sp.) is one type of  lobsters that has been cultivated in Indonesia. One obstacle is the lobster stocking density. Stocking density is one of the key factors to achieve optimal growth, it is associated with intensive cultivation to obtain high production. This study aimed to assess the effect of stocking density on survival rate, consumption and efficiency of feed utilization and growth rate of juvenile stadia freshwater crayfish (Cherax sp.). The research was implemented in October-December 2013 in the Wet Laboratory of Aquaculture, Department of Fisheries, Faculty of Fisheries and Marine Science, Diponegoro University. Semarang. Cultivan used in this research is Freshwater Lobster (Cherax sp.) aged 40 days. Using a completely randomized design (CRD) with 3 replications that treatment A (200 ekor/m2 stocking density), B (250 lobsters/m2 stocking density), C (300 lobsters/m2 stocking density), D (300 lobsters/m2 stocking density). The variables measured were blood glucose, the level of feed intake, feed utilization efficiency, growth, survival and quality of water. The results showed there was effect (P<0.05) stocking density against survival rate, feed utilization efficiency and growth rate juvenil Freshwater Lobster (Cherax sp.). Treatment A (stocking density 200 lobsters/m2) had the highest result on the efficiency of feed utilization is 64.50±1.23% , the growth rate of which is 1.81 ± 0.02 % and the survival rate is 90.28±2.41% . While on treatment D (stocking density 350 lobsters/m2) had the lowest result efficiency of feed utilization is 41.80±0.56%, 1.02±0.00% growth and survival 65.87±1.37%. The best stocking density for growth and survival of juvenile freshwater crayfish is 250 lobsters/m2.
Evaluation of Antibacterial and Antibiofilm Effects from Soil Streptomyces spp. against Multidrug-Resistant Bacteria Prastya, Muhammad Eka; Simbolon, Sumihartati; Priyanto, Jepri Agung; Hasidu, La Ode Abdul Fajar; Permatasari, Vera; Primahana, Gian; Dewi, Rizna Triana; Kristiana, Rhesi; Suryanti, Erma
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.12-26

Abstract

The global increase in multidrug-resistant (MDR) bacterial infection has rapidly gained concern globally. This study aimed to investigate antibacterial and antibiofilm potential of 25 soil actinomycete strains against MDR strains including Escherichia coli strain M4, Pseudomonas aeruginosa strain M19, Klebsiella pneumoniae strain M19, Bacillus subtilis strain M18, and Methicillin Resistant Staphylococcus aureus (MRSA). In this study, three actinomycete isolates encoded APM-7, APM-11, and APM-21 showed a strong and broad antibacterial spectrum. The minimum inhibitory concentration (MIC) of extracts derived from these isolates was ranged from 78 μg/ml to 10,000 μg/ml. In addition, The extracts also displayed significant biofilm inhibition values ranging from 6.06 to 72.4%. Based on the results, APM-21 extract had the best antibacterial and antibiofilm activities with the strongest values against MRSA. According to the nucleotide sequencing of the 16S rRNA gene, APM-7, APM-11, and APM-21 strains possessed similar identities with Streptomyces cyaneus, Streptomyces coerulescens, and Streptomyces panayensis, respectively. Based on Liquid Chromatography Tandem-Mass Spectrometry (LC-MS/MS) analysis, two antibacterial compounds, namely rancimanycin III, and enteromycin were detected in all those three extracts. Interestingly, APM-21 extract also contained two prominent antibacterial substances including paramagnetoquinone C, and caerulomycin I, suggesting their contribution to the most potential activities. Moreover, new insights were provided into a promising candidate for use in an active compound combating strategy to control MDR bacterial strain infection.