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All Journal HAYATI Journal of Biosciences Jurnal Sumberdaya Hayati Jurnal Riset Biologi dan Aplikasinya BioEksakta : Jurnal Ilmiah Biologi Unsoed Jurnal Abdimas Berdaya : Jurnal Pembelajaran, Pemberdayaan dan Pengabdian Masyarakat TeknoKreatif: Jurnal Pengabdian kepada Masyarakat Bioeksperimen
Suryanti, Erma
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Aksi Bina Kesehatan Reproduksi dengan Potensi Tanaman Obat Keluarga di Kelurahan Way Dadi, Bandar Lampung Sitanggang, Fajar Islam; Riana, Elisa Nurma; Wijayanti, Hendry; Suryanti, Erma
TeknoKreatif: Jurnal Pengabdian kepada Masyarakat Vol 3 No 1 (2023): TEKNOKREATIF : Jurnal Pengabdian kepada Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M), Institut Teknologi Sumatera, Lampung, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35472/teknokreatif.v3i1.739

Abstract

Reproductive health is one of the knowledge that is still taboo in society. In fact, it is very important for reproductive development. In adolescents, physical, mental, and social changes must be balanced with good and correct knowledge. Thus, a healthy and quality generation can be created. Way Dadi Village, is one of the villages located in Sukarame District, Bandar Lampung City with a fairly high number of teenagers and adults. This Community Service (PKM) aims to provide knowledge to the public about the importance of reproductive health in maintaining the quality of generations and provide knowledge about the importance of knowing the potential of Medicinal Plants (TOGA) which can be used as prevention of reproductive diseases. This PKM is carried out with a face-to-face method in accordance with health protocols. The PKM method is carried out by evaluating the pre-instruction and post-instruction research questionnaires. Filling in is based on the weighting of understanding starting from not, enough, moderate, good, and very good. Thus, the final post-exam results are obtained with a very good average understanding of up to 86%.
In Vitro Evaluation of Phosphate-Solubilizing Bacteria as Antagonists for Bacterial Leaf Blight Disease (Xanthomonas oryzae pv. oryzae) Asril, Muhammad; Suryanti, Erma; Agus Rini, Ika
Jurnal Riset Biologi dan Aplikasinya Vol. 6 No. 1 (2024)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v6n1.p34-40

Abstract

Rice is an important food crop in Indonesia, where more than 50% of the Indonesian population consumes rice as a staple food. Rice has decreased productivity by 50-80% due to bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae (Xoo). The rapid spread and damaging effects of pesticide use on the environment are the main obstacles in the treatment of these bacteria. Alternative control is to use biological agents in the form of phosphate solubilizing bacteria (PSB). Phosphate-solubilizing bacteria are part of the growth-promoting bacteria that have other abilities, namely biocontrol. The purpose of this study was to develop the ability of acid soil phosphate solubilizing bacteria to inhibit growth in vitro. The test was carried out using the dual culture technique with the zone of inhibition method. The results showed that ten isolates of PSB had inhibition against Xoo with various diameters of inhibition. The four highest inhibitory isolates were EF.NAP 3 (5.267 ± 0.189 mm), Pseudomonas GSP 6 (4533 ± 0.772 mm), EF.NAP 4 (3,424 ± 0.161 mm) and EF. NAP 9 (3.167±0.136 mm). The ability of PSB to inhibit Xoo can be used as an alternative in controlling bacterial leaf blight in rice plants. This indicates that the PSB isolate acts as a biostimulant in the dissolution of phosphate in the soil and as a bioprotectant.
Isolasi dan Karakterisasi Bakteri Rhizobium asal Bintil Akar Tanaman Kacang Tanah (Arachis hypogaea) dan Koro Rawe (Mucuna bracteata): Isolation and Characterization of Rhizobium Bacteria from Root Nodules of Peanut Plants (Arachis hypogaea) and Koro Rawe (Mucuna bracteata) Nabilla, Alnovara Yuan; Prastya, Muhammad Eka ; anggria sari, Dian; Suryanti, Erma
Jurnal Sumberdaya Hayati Vol. 10 No. 4 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.4.175-182

Abstract

Nitrogen (N) is naturally available in the form of N2. Nitrogen fixation can be facilitated by microbes such as Rhizobium bacteria, which can establish symbiosis with the roots of legume plants to form root nodules that perform nitrogen fixation. This study aims to determine the characteristics of Rhizobium bacteria in the root nodules of peanuts (Arachis hypogaea) and koro rawe (Mucuna bracteata). The methods used in this research include collecting root nodules, isolating Rhizobium bacteria, and identifying and characterizing these bacteria for their nitrogen fixation and phosphate solubilization capabilities. Characterization results on YEMA + Congo red media indicated that 10 out of 16 isolates from peanut root nodules and 30 out of 55 isolates from koro rawe root nodules were positive for Rhizobium bacteria. The nitrogen fixation activity test revealed that only 4 isolates from peanut root nodules and 6 from koro rawe root nodules exhibited nitrogen fixation ability in Jensen's medium. The highest phosphate solubilization index was obtained from isolate KK.5.1.1 (0.5±0.08), while the lowest was from isolate KK.5.2.2 (0.09±0.06). This research underscores the significance of Rhizobium in enhancing nitrogen availability and phosphate solubilization for legume plants.
Pemeliharaan Lebah Tanpa Sengat Sebagai Usaha Peningkatan Imunitas Tubuh Masyarakat Bumisari, Natar Sari, Dian Anggria; Mulyana, Jeane Siswitasari; Riana, Elisa Nurma; Suryanti, Erma; Nurhayu, Winati; Maretta, Gres; Antonius, Antonius; Zahra, Anisa Mumtaz; Nurcahya, Gebriel
TeknoKreatif: Jurnal Pengabdian kepada Masyarakat Vol 4 No 2 (2024): TEKNOKREATIF : Jurnal Pengabdian kepada Masyarakat Volume 4 No 2
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M), Institut Teknologi Sumatera, Lampung, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35472/teknokreatif.v4i2.1121

Abstract

ABSTRAK Pandemi global mengharuskan masyarakat untuk memberikan perhatian lebih terhadap peningkatan daya tahan tubuh agar tidak mudah terpapar berbagai penyakit terutama Covid-19. Ada banyak cara yang dapat dilakukan untuk meningkatkan imunitas tubuh, salah satunya dengan mengkonsumsi makanan dari produk alami bebas bahan kimia berbahaya yang mengandung nutrisi seimbang. Salah satu produk alami yang dapat meningkatkan daya tahan tubuh adalah madu. Madu merupakan salah satu produk alami lebah yang dapat langsung dikonsumsi dan berkhasiat untuk kesehatan. Lebah yang umumnya dipelihara untuk menghasilkan madu adalah jenis lebah madu Apis sp. yang memiliki sengat. Hal ini yang menyebabkan madu hanya bisa diperoleh dari peternak lebah dan distributor madu. Namun, jenis lebah liar tanpa sengat dapat mempermudah masyarakat untuk memperoleh madu dan dapat dipelihara langsung dari rumah. Pengetahuan pemeliharaan lebah madu dapat membantu masyarakat untuk memastikan tersedianya asupan nutrisi yang dapat menjaga daya tahan tubuh keluarga. Pelaksanaan pelatihan pemeliharaan lebah tanpa sengat dilaksanakan dengan metoda ceramah di Desa Bumisari, Natar, Lampung Selatan. Tahapan pelaksanaan kegiatan ini meliputi pengisian kuesioner pre-test dan post-test untuk mengetahui pengetahuan masyarakat sebelum dan sesudah dilakasanakan pelatihan. Ketercapaian target diketahui dapat berdasarkan hasil analisis kuesioner yang dilakukan, dimana ditemukan peningkatan pemahaman masyarakat hingga 87.096%. Berdasarkan hasil akhir kegiatan, diharapkan masyarakat semakin baik mengelola lingkungan sekitar sehingga sumber tanaman penghasil nektar untuk madu lebah tanpa sengat tetap terus tersedia. Kata kunci: daya tahan tubuh, lebah tanpa sengat, madu, desa Bumisari ABSTRACT People need to focus more on strengthening their immune systems in order to avoid being easily exposed to numerous diseases, including COVID-19. There are many things that can be done to increase the body's immunity, one of which is by consuming foods derived from natural products that free of harmful chemicals and contain a balanced of nutrients. Honey is one of the natural bee products that can be consumed right away and is good for human health. Apis spp., is the common insect species of bees with sting, are typically kept to generate honey. Because of this, good quality honey is only available from professional beekeepers and honey suppliers. However, wild stingless bees can help people to obtain honey and can be kept at home. Knowledge of honey bee maintenance can help the community to ensure the availability of nutritional intake that can maintain the family's immune system. The implementation of stingless beekeeping training was carried out using the lecture method in Bumisari Village, Natar, South Lampung. The stages of implementing this activity include filling out pre-test and post-test questionnaires to determine community knowledge before and after the training. The achievement of the target can be known based on the results of the questionnaire analysis conducted, which found an increase in public understanding of up to 87.096%. Based on the final results of the activity, it is hoped that the community will better manage the surrounding environment so that sources of nectar-producing plants for stingless bee honey will continue to be available. Keywords: immune system, stingless bee, honey, Bumisari village
Evaluation of Antibacterial and Antibiofilm Effects from Soil Streptomyces spp. against Multidrug-Resistant Bacteria Prastya, Muhammad Eka; Simbolon, Sumihartati; Priyanto, Jepri Agung; Hasidu, La Ode Abdul Fajar; Permatasari, Vera; Primahana, Gian; Dewi, Rizna Triana; Kristiana, Rhesi; Suryanti, Erma
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.12-26

Abstract

The global increase in multidrug-resistant (MDR) bacterial infection has rapidly gained concern globally. This study aimed to investigate antibacterial and antibiofilm potential of 25 soil actinomycete strains against MDR strains including Escherichia coli strain M4, Pseudomonas aeruginosa strain M19, Klebsiella pneumoniae strain M19, Bacillus subtilis strain M18, and Methicillin Resistant Staphylococcus aureus (MRSA). In this study, three actinomycete isolates encoded APM-7, APM-11, and APM-21 showed a strong and broad antibacterial spectrum. The minimum inhibitory concentration (MIC) of extracts derived from these isolates was ranged from 78 μg/ml to 10,000 μg/ml. In addition, The extracts also displayed significant biofilm inhibition values ranging from 6.06 to 72.4%. Based on the results, APM-21 extract had the best antibacterial and antibiofilm activities with the strongest values against MRSA. According to the nucleotide sequencing of the 16S rRNA gene, APM-7, APM-11, and APM-21 strains possessed similar identities with Streptomyces cyaneus, Streptomyces coerulescens, and Streptomyces panayensis, respectively. Based on Liquid Chromatography Tandem-Mass Spectrometry (LC-MS/MS) analysis, two antibacterial compounds, namely rancimanycin III, and enteromycin were detected in all those three extracts. Interestingly, APM-21 extract also contained two prominent antibacterial substances including paramagnetoquinone C, and caerulomycin I, suggesting their contribution to the most potential activities. Moreover, new insights were provided into a promising candidate for use in an active compound combating strategy to control MDR bacterial strain infection.
Pelatihan Pemanfaatan Limbah Minyak Jelantah menjadi Produk Lilin Aromaterapi di Desa Pahawang, Pesawaran Khairani, Iffa Afiqa; Anbar Istiadi, Khaerunissa; Chusniasih, Dewi; Suryanti, Erma; Arliani Nur Anisa, Hida; Natalia Pardede, Desta
Jurnal Abdimas Berdaya : Jurnal Pembelajaran, Pemberdayaan dan Pengabdian Masyarakat Vol 8, No 1 (2025): Jurnal Abdimas Berdaya
Publisher : Universitas Islam Lamongan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30736/jab.v8i1.1040

Abstract

Isolasi dan Karakterisasi Bakteri Termofililk Penghasil Enzim Protease Asal Sumber Air Panas Way Belerang, Lampung Suryanti, Aulia Putri; Suryanti, Erma; Asagabaldan, Meezan Ardhanu
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 3 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.3.16255

Abstract

Protease merupakan enzim yang menghidrolisis ikatan peptida pada protein. Enzim ini biasa dimanfaatkan dalam industri pangan, pertanian, medis hingga membantu dalam proses pencernaan. Pencarian enzim protease dari bakteri diperlukan terutama bakteri yang memiliki ketahanan terhadap suhu tinggi, seperti bakteri termofilik. Bakteri termofilik dapat hidup pada suhu diatas 45ºC dan dapat hidup optimal pada suhu antara 50ºC-65ºC. Bakteri termofilik dapat diperoleh salah satunya dari Sumber Air Panas Way Belerang yang terletak di Kalianda, Lampung Selatan. Penelitian ini memiliki tujuan untuk mengisolasi bakteri termofilik asal Sumber Air Panas Way Belerang, mengkarakterisasi bakteri termofilik penghasil enzim protease, serta menguji aktivitas enzim protease secara kualitatif dan kuantitatif dari bakteri termofilik asal pemandian air panas Way Belerang. Metode penelitian ini terdiri atas isolasi sampel bakteri dari sedimen dan air dari Way Belerang, uji kualitatif enzim protease bakteri termofilik, karakterisasi makroskopik dan mikroskopik bakteri penghasil enzim protease, kurva pertumbuhan bakteri, pengujian aktivitas enzim protease secara kuantitatif, serta analisis data. Hasil penelitian diperoleh 26 isolat bakteri hasil isolasi dari Sumber Air Panas Way Belerang yang diperoleh dari sampel air dan sedimen. Isolat tersebut diujikan aktivitas protease secara kualitatif dan kuantitatif. Secara kualitatif diperoleh 11 dari 26 isolat yang terdapat aktivitas proteolitik pada pengujian di suhu 53 oC dan pada media NA + 1 % skim milk powder. Isolat S5.1 memiliki Indeks proteolitik tertinggi sebesar 3.59 yang ditandai dengan adantyz zona bening di sekitar koloni bakteri pada media media NA + 1 % skim milk powder. Aktivitas protease secara kuantitatif sebesar 0.0204 unit/ml pada suhu 53 oC Karakterisasi morfologi menunjukkan bakteri A9.5 adalah Gram positip. Bakteri termofilik S5.1 berpotensi sebagai penghasil enzim protease dan perlu dikarakterisasi lebih lanjut aktivitasnya dengan variasi pH dan suhu untuk mendapatkan aktivitas enzim yang optimal untuk aplikasi industri yang memerlukan enzim termostabil. Kata kunci : Bakteri termofililk, enzim protease, Sumber air panas Way Belerang
Isolation and screening of halotolerant protease-producing bacteria from Lampung fermented shrimp paste Istiadi, Khaerunissa Anbar; Ningsih; Suryanti, Erma; Imaniar, Lisana Husna
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.14593

Abstract

Protease are class of degradative enzymes that hydrolyze proteins, breaking its peptide bond, into smaller peptides and amino acids. Microbial proteases have gained increasing attention due to its productivity and utilization in various industries. Proteolytic bacteria derived from fermented shrimp paste from Lampung have great potential to meet this need. However, studies on these bacteria are still limited, so further research is needed to explore and optimally utilize their potential. This study aims to explore the diversity of proteolytic bacteria from Lampung terasi.. The methods employed included isolation stages using Nutrient Agar supplemented with 5% and 10% NaCl (w/v), followed by proteolytic screening properties using Skim Milk Agar media. A total of 20 bacterial isolates were obtained from terasi using Nutrient Agar supplemented with 5% (w/v) NaCl, and 15 isolates were obtained from Nutrient Agar (NA)  supplemented with 10% (w/v) NaCl. Screening results showed that 85% and 67% of the isolates obtained from NA supplemented with 5% and 10% NaCl, respectively, exhibited proteolytic activity. Halotolerant bacterial isolates isolated using NA + 5% NaCl exhibited a proteolytic index ranging from 0.3 – 3.0, while those isolated with NA + 10% NaCl showed values ranging from 0.18 to 3.06. The screening results revealed that isolates BTCL5-07 and BTCL10-05 exhibited the highest proteolytic activity, with proteolytic indices of 3.0 and 3.06, respectively. These findings indicate that halotolerant bacteria from Lampung terasi represent a promising source of potential proteolytic enzymes. Further research is required to optimize enzyme production.
Co-Authors Agus Rini, Ika Anbar Istiadi, Khaerunissa anggria sari, Dian Antonius Antonius Arliani Nur Anisa, Hida Asagabaldan, Meezan Ardhanu Asril, Muhammad Dewi Chusniasih Gres Maretta, Gres Hasidu, La Ode Abdul Fajar Iffa Afiqa Khairani Imaniar, Lisana Husna Jepri Agung Priyanto, Jepri Agung Khaerunissa Anbar Istiadi Mulyana, Jeane Siswitasari Nabilla, Alnovara Yuan Natalia Pardede, Desta Ningsih Nurcahya, Gebriel Nurhayu, Winati Permatasari, Vera Prastya, Muhammad Eka Prastya, Muhammad Eka  Primahana, Gian Rhesi Kristiana Riana, Elisa Nurma Rizna Triana Dewi Sari, Dian Anggria Simbolon, Sumihartati Sitanggang, Fajar Islam Suryanti, Aulia Putri Wijayanti, Hendry Zahra, Anisa Mumtaz