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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Dasar Jurnal Sumberdaya Hayati Molekul: Jurnal Ilmiah Kimia
Permatasari, Vera
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Earth & Planetary Sciences Immunology & microbiology Materials Science & Nanotechnology Mathematics

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Antioxidant and Cytotoxic Properties of Extract from Soil Bacteria Isolated from Muna Island, Southeast Sulawesi Priyanto, Jepri Agung; Hening, Egiyanti Nur Widhia; Permatasari, Vera; Prastya, Muhammad Eka; Hasidu, La Ode Abdul Fajar; Primahana, Gian
Jurnal ILMU DASAR Vol 25 No 1 (2024)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v25i1.39244

Abstract

Soil bacteria could be the promising source of secondary metabolites with diverse pharmacological activities. Bacteria coded as P1 and M7 were isolated from soil obtained from Muna Island, Southeast Sulawesi. This research aimed to evaluate the antioxidant activity, cytotoxic property, and identify the chemical profile of the extract derived from soil bacteria P1 and M7. Based on 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, extract derived from P1 isolate was moderately active against ABTS free radical with IC50 of 126.22±10.82 µg/mL, and less active on DPPH (IC50: 721.95±16.59 µg/mL), while M7 extract was less active towards DPPH and ABTS. Interestingly, these extracts in the concentration of 100 µg/mL were cytotoxic to breast cancer cell (MCF-7), as tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. MCF-7 viability was decreased up to 69.83±1.46 % and 61.49±0.76 % after treated with P1 and M7 extract, respectively. The extracts were also induced membrane disruption and apoptosis of MCF-7 cells. The extracts contained phenol (9.52±0.22 and 10.41±0.09 mg EAG/g extract, for P1 and M7, respectively) and flavonoids (9.16±0.16 and 9.29±0.24 mg EQ/g extract, for P1 and M7, respectively). According to Gas Chromatography Mass Spectrometry (GC-MS) analysis, Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) that previously well known as antioxidant and cytotoxic compounds, were found in the M7 and P1 extract. This compound may play a vital role in the radical scavenging and cytotoxic properties of P1 and M7 extracts. The results of this study indicate that P1 and M7 extracts could be the potential source of antioxidant and cytotoxic compounds and need to be further developed for pharmaceutical use.
Uji Aktivitas Antibakteri dan Antioksidan dari Metabolit Ekstraseluler Bakteri Endofit Tumbuhan Mahang (Macaranga bancana): Antibacterial and Antioxidant Activity Test of Extracellular Metabolites of Endophytic Bacteria of Mahang Plants (Macaranga bancana) Chang, Helen; Pujiyanto, Sri; Prastya, Muhammad Eka; Primahana, Gian; Yuswan, Apriza; Retnowati, Dwi; Permatasari, Vera
Jurnal Sumberdaya Hayati Vol. 10 No. 4 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.4.190-197

Abstract

The increasing pollution which promotes accumulation of free radicals and a significant number of bacterial resistances to antibiotics have prompted various efforts to search for new sources of bioactive compounds. The aim of this study was to investigate the antibacterial and antioxidant properties of bioactive compounds produced by endophyte bacteria from M. bancana. The most potential bacterial isolate was extracted its active constituents until obatined crude extract and tested using disc diffusion technique and detemined MIC and MBC values. Antioxidant properties using DPPH radicals were performed followed by GC-MS analysis of its crude extract. We obtained that MB3.1 isolate exhibited antibacterial activity against all four tested bacteria including Escherichia coli strain ATCC 8739, Pseudomonas aeruginosa strain ATCC 9027, Staphylococcus aureus strain ATCC 25923, and Bacillus subtilis strain ATCC 6633. The best MIC values of this extract were shown against B. subtilis with an MIC value of 39.06 μg/ml. Further, MB3.1 extract showed a moderate antioxidant activity with an IC50 value of 248.19 μg/ml. GC-MS analysis of MB3.1 extract revealed 10 dominant compounds, including phenylethyl alcohol, benzeneethanol, 4-hydroxy, and tryptophol, which might be responsible for the antibacterial and antioxidant properties.
Evaluation of Antibacterial and Antibiofilm Effects from Soil Streptomyces spp. against Multidrug-Resistant Bacteria Prastya, Muhammad Eka; Simbolon, Sumihartati; Priyanto, Jepri Agung; Hasidu, La Ode Abdul Fajar; Permatasari, Vera; Primahana, Gian; Dewi, Rizna Triana; Kristiana, Rhesi; Suryanti, Erma
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.12-26

Abstract

The global increase in multidrug-resistant (MDR) bacterial infection has rapidly gained concern globally. This study aimed to investigate antibacterial and antibiofilm potential of 25 soil actinomycete strains against MDR strains including Escherichia coli strain M4, Pseudomonas aeruginosa strain M19, Klebsiella pneumoniae strain M19, Bacillus subtilis strain M18, and Methicillin Resistant Staphylococcus aureus (MRSA). In this study, three actinomycete isolates encoded APM-7, APM-11, and APM-21 showed a strong and broad antibacterial spectrum. The minimum inhibitory concentration (MIC) of extracts derived from these isolates was ranged from 78 μg/ml to 10,000 μg/ml. In addition, The extracts also displayed significant biofilm inhibition values ranging from 6.06 to 72.4%. Based on the results, APM-21 extract had the best antibacterial and antibiofilm activities with the strongest values against MRSA. According to the nucleotide sequencing of the 16S rRNA gene, APM-7, APM-11, and APM-21 strains possessed similar identities with Streptomyces cyaneus, Streptomyces coerulescens, and Streptomyces panayensis, respectively. Based on Liquid Chromatography Tandem-Mass Spectrometry (LC-MS/MS) analysis, two antibacterial compounds, namely rancimanycin III, and enteromycin were detected in all those three extracts. Interestingly, APM-21 extract also contained two prominent antibacterial substances including paramagnetoquinone C, and caerulomycin I, suggesting their contribution to the most potential activities. Moreover, new insights were provided into a promising candidate for use in an active compound combating strategy to control MDR bacterial strain infection.
Potensi Aktivitas Antibakteri dan Antioksidan dari Senyawa Bioaktif Bakteri Tanah Asal Samarinda, Kalimantan Timur: The Antibacterial and Antioxidant Potential of Bioactive Metabolites from Soil-Derived Bacteria in Samarinda, East Kalimantan Atwita, Syelziva Yonda; Malau, Jekmal; Permatasari, Vera; Primahana, Gian; Dewijanti, Indah D.; Yuswan, Apriza; Prastya, Muhammad Eka
Jurnal Sumberdaya Hayati Vol. 11 No. 2 (2025): 2025
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.11.2.63-73

Abstract

Antibiotic resistance is a global challenge, especially in the treatment of bacterial infections. This study aims to explore the antibacterial and antioxidant potential of bioactive compounds isolated from soil bacteria in Samarinda, East Kalimantan. Of the 30 isolates tested, 1 potential isolate was obtained, namely isolate code T1.16. This potential bacterial isolate was further cultured and fermented in Tryptic Soybean and Luria Bertani Broth medium to obtain its crude secondary metabolite. Interestingly, its crude extract showed antibacterial activity with MIC values of 100.4-3,211 µg/ml against Escherichia coli strain ATCC 8739, Pseudomonas aeruginosa strain ATCC 9027, Bacillus subtilis strain ATCC 6633, and Staphylococcus aureus strain ATCC 25923. Based on 1,1-diphenyl-2-picrylhydrazyl (DPPH) method was used to identify antioxidant activity, IC50 values obtained ranged from 990-1730 µg/ml. Ultimately, by using Gas Chromatography Mass Spectrometry (GC-MS) analysis, bioactive compounds in the form of Cyclo(L-prolyl-L-valine), 2-methylpropyl and phenylmethyl in bacterial extract T1.16 have potential antioxidant and antibacterial bioactivities. These results support the potential development of new antibacterial and antioxidant compounds from soil bacteria, as well as the importance of further exploration for pharmacological applications.
Antioxidant, Anti-Aging and Antibacterial Activity from Dewa Leaves Ethanolic Extract (Gynura japonica (Thunb.) Juel) Fatmawati, Umi; Zienitha, Anggie Meilinda; Anggraini, Meisi; Rosita, Lisa; Indrowati, Meti; Harlita, Harlita; Santosa, Slamet; Permatasari, Vera; Primahana, Gian; Yati, Indri; Prastya, Muhamad Eka
Molekul Vol 20 No 2 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.2.13792

Abstract

ABSTRACT. Due to their phytochemical constituents, Dewa leaves (Gynura japonica (Thunb.) Juel.) are often used in traditional medicinal herbs. However, in vitro and in vivo of antioxidative and anti-aging studies of Dewa leaves on yeast as a eukaryotic cell model have not been widely carried out. This study aims to determine the antioxidant, anti-aging, and antibacterial activities derived from G. japonica leaves extract. Extraction was conducted using 70% and 96% ethanol solvents, total phenolic content (TPC) was assayed using Folin-Ciocalteu method, flavonoid contents (TFC) was assayed using aluminum chloride method, antioxidant activity was tested using DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2-azinobis-3-Ethylbenzothiazoline)-6-sulfonic acid) radicals. Subsequently, an anti-aging activity test was performed on the yeast Saccharomyces cerevisiae as a model organism following antibacterial activity. The antibacterial activity test was carried out using well diffusion agar, and the Minimal Inhibitory Concentration (MIC) was determined using the microplate method. We obtained that G. japonica leaves extracted from 70% and 96% methanol solvents, have TPC of 13.14 and 22.11 mg GAE/gr extract, and TFC of 8.04 and 14.09 mg QE/gr extract, respectively. As for DPPH and ABTS antioxidant activity, D70 showed the best activity with IC50 values of 1411.36±56.35 µg/mL and 2516.10±18.77 µg/mL, respectively. The anti-aging test showed that both 70% and 96% ethanol extracts were able to maintain the yeast cell viability under H2O2 oxidative stress. Further, 70% and 96% ethanol extract also showed antibacterial activity at the best value against Staphylococcus aureus with a MIC value of 390.62 µg/mL, it means that Gynura leaves extract has a potency as antibacterial agent. The results of this study indicate that the ethanol extract of G. japonica leaves can be developed for further investigation as an antioxidant and antibacterial therapeutic agent. Keywords: Anti-aging, antibacterial, antioxidant, Gynura japonica, Saccharomyces cerevisiae
Evaluasi Aktivitas Antioksidan dan Antibakteri Dari Daun Empat Tumbuhan Suku Baduy Sebagai Sumber Bahan Alam Potensial Permatasari, Vera; Riskiana, Angger Arfi; Saefurohman, Asep; Prastya, Muhammad Eka; Ridha, Rafifa; Primahana, Gian; Dewijanti, Indah Dwiatmi
Jurnal Sumberdaya Hayati Vol. 11 No. 3 (2025): 2025
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.11.3.122-129

Abstract

Baduy indigenous plants have great potential as natural sources of bioactive compounds, yet scientific studies on their biological activities remain limited. This study aimed to evaluate the antioxidant, antibacterial activities, and total phenolic content of the leaves from four Baduy plant species: Gardenia jasminoides, Strobilanthes crispus, Ficus callosa, and Artocarpus camansi. The leaves were extracted with methanol, followed by fractionation using n-hexane and ethyl acetate. Antioxidant activity was assessed using DPPH and ABTS assays, total phenolic content was determined by the Folin–Ciocalteu method, and antibacterial activity was tested against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli using the broth dilution method to determine Minimum Inhibitory Concentration (MIC). The results showed that the ethyl acetate fraction of A. camansi leaves exhibited the highest total phenolic content (23.53 mg GAE/g extract). This fraction demonstrated strong antioxidant activity in the ABTS assay (IC50 = 18.55 ppm) and moderate activity in the DPPH assay (IC50 = 50.67 ppm). Moreover, it showed strong antibacterial activity against S. aureus, B. subtilis, and P. aeruginosa. These findings indicate that A. camansi leaves possess promising potential as a natural source of antioxidant and antibacterial agents.
Co-Authors Anggraini, Meisi Asep Saefurohman Atwita, Syelziva Yonda Chang, Helen Dewijanti, Indah D. Dewijanti, Indah Dwiatmi Dwi Retnowati Harlita Harlita Hasidu, La Ode Abdul Fajar Hening, Egiyanti Nur Widhia Jekmal Malau Jepri Agung Priyanto, Jepri Agung Meti Indrowati Prastya, Muhamad Eka Prastya, Muhammad Eka Primahana, Gian Rhesi Kristiana Ridha, Rafifa Riskiana, Angger Arfi Rizna Triana Dewi Rosita, Lisa Simbolon, Sumihartati Slamet Santosa Sri Pujiyanto Suryanti, Erma UMI FATMAWATI yati, indri Yuswan, Apriza Zienitha, Anggie Meilinda