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Kajian Fisiologis Status Kalsium Puyuh (Coturnix Coturnix Japonica) setelah Pemberian Cahaya Monokromatik Kasiyati, Kasiyati; Kusumorini, Nastiti; Maheshwari, Hera; Manalu, Wasmen
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 18, No 1 (2010): Vol. XVIII, No. 1, Maret 2010
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Deposited calsium makes of the eggshell is influnce of the light. The objective of the study was evaluate exposed of monochromatic light to obtain basic information of the physiology aspect of calcium satus in the quail which direct relation with quality of the eggshell. Two hundred and seventy quails were divided into nine treatments of light, with ten replications and three quails in each replication. The treatments were without light, controls with 15 and 25 W, red, green, and blue lights with intensities of 15 and 25 lux. Control treatment used incandescent bulb. The red, green, and blue lights were provided by light emitting diodes (LED). All lights treatment were given for 14 h daily, started from 17.00 to 07.00. Parameters measured were serum calcium concentrations, calcium contents in bone and the eggshell, weights and thichness of the eggshell. The data obtained were analyzed by analysis of variance (ANOVA), and the differences between treatments groups were subjected for Duncanâ€™s Multiple Range Test (DMRT). All of the data analyzed were done GLM (general linear model) procedure in the SAS program. Quails exposed to monochromatic light had higher serum calcium concentrations and calcium contents in bone (P<0,05). Blue light could be used to increase quality of the eggshell.

Penerapan Cahaya Monokromatik untuk Perbaikan Kuantitas Telur Puyuh (Coturnix coturnix japonica.L) Kasiati, Kasiyati; Kusumorini, Nastiti; Maheshwari, Hera; Manalu, Wasmen
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 19, No 1 (2011) : Volume XIX, Nomor 1, Maret 2011
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

The quailâ€™s egg have value of nutrient which similar to the chickenâ€™s egg and the duckâ€™s egg. Various of the programme of the light to improve repair of the quantity and the quality of the quail egg. The objectives of this research were to applied monochromatic light to repair the quality and quantity of the quail egg. Two hundred and seventy female quails (DOQ) were divided into nine treatments of light, with ten replications and three quails in each replication, the treatments were without light, controls with 15 and 25 W, red, green, and blue lights with intensities of 15 and 25 lux. Control treatment used incandescent bulb. The red, green, and blue lights were provided by light emitting diodes (LED). All lights treatment were given for 14 h daily, started from 17.00 to 07.00. Parameters measured were amount of the egg weekly, henday egg production, and quality of the egg consist of the weights of the egg, protein and fat of the egg. The result showed that quails exposed to blue light had higher of the amount of the egg, henday production, and the weights of the egg, while the quality of the egg, such as protein and fat in the egg were not change (P>0,05). Blue light could be used to improve repairing quantities of the quail egg.

KADAR ESTROGEN DAN PROFIL OVIDUK PUYUH (Coturnix coturnix japonica) SETELAH PEMBERIAN CAHAYA MONOKROMATIK Kasiyati, Kasiyati; Kusumorini, Nastiti; Maheshwari, Hera; Manalu, Wasmen
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 17, No 2 (2009): Volume XVII, Nomor 2, Oktober 2009
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Light plays an important role in the avian life. The present study was designed to evaluate the effects of monochromatic light on serum estrogen concentrations and profile the oviduct, that were the length and weight of the oviduct in the quail. Two hundred and seventy quails were divided into nine treatments of light, with ten replications and three quails in each replication. The treatments were without light,Â controlsÂ with 15 and 25 W, red, green, and blue lights with intensities ofÂ 15 and 25 lux. ControlÂ treatment used incandescent bulb. Â The red, Â green, Â and Â blue lights Â were Â provided Â byÂ Â light emittingÂ Â diodes Â (LED). All lightsÂ Â treatment Â Â wereÂ Â given Â Â forÂ Â 14 h Â daily,Â Â started from 17.00 to 07.00. ParametersÂ Â measuredÂ wereÂ serum estrogenÂ concentrations, andÂ weightsÂ andÂ lengthsÂ of Â Â theÂ oviductÂ onÂ weeks 5, Â 7,Â and Â 9.Â QuailsÂ Â exposed Â toÂ monochromatic Â light had Â higher Â serum Â estrogen Â concentrations (P<0,05). Quails Â exposed to monochromatic light had Â better Â weightsÂ and Â lenghts of Â the Â oviduct Â development. Blue light could be used to increase serum estrogen concentrations and stimulate shell gland development.

Nilai Indeks Aterogenik Plasma (IAP) Dan Indeks Castelli (IC) Mencit Model yang Diinduksi Minyak Trans Supiyani, Atin; Sukmawati, Dalia; Kusumorini, Nastiti; Santoso, Koekoeh; Satyaningtijas, Aryani Sismin
Jurnal MIPA Vol 10, No 2 (2021)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jmuo.10.2.2021.34094

dikembangkan melalui pendekatan hewan model untuk mempelajari tentang PJK. Minyak goreng yang digunakan secara berulang dapat meningkatkan kadar asam lemak bebas yang berdampak negatif bagi kesehatan terutama jantung dan pembuluh. Tujuan dari penelitian ini mengukur indeks aterogenik plasma (IAP) dan indeks Castelli (IC) dari mencit model yang diinduksi minyak trans (MT). Sebanyak 16 ekor mencit galur DDY dibagi menjadi 4 kelompok yaitu mencit yang diinduksi 20% minyak trans (MT20), 40% minyak trans (MT40) dan 60% minyak trans (MT60) dan kontrol (K). Persentase minyak trans diberikan berdasarkan dari total energi konsumsi pakan harian. Minyak trans diberikan per oral sehari sekali selama 70 hari. Darah diambil pada hari ke-70 dari vena lateral ekor tikus untuk mengukur profil lipid plasma yaitu kolesterol total, trigliserida (TG), High Density Lippoprotein (HDL) dan Low Density Lipoprotein (LDL) plasmaÂ menggunakan alat uji cepat Lipid ProÂ®. Nilai Indeks Aterogenik Plasma (IAP) yaitu log (TG/HDL) dan Indeks Castelli (IC) yaitu LDL/HDL. Hasil pengkuran profil lipid plasma menunjukkan kolesterol total, trigliserida, HDL dan LDL plasma pada kelompok MT meningkat secara nyata dibandingkan kontrol (sig<0,05). Nilai IA pada mencit yang diberi minyak trans berbeda nyata dari kontrol (sig<0,05) dan memiliki risiko tinggi. Nilai IC pada mencit yang diberi minyak trans berbeda nyata dari kontrol (sig<0,05) dan memiliki risiko sedang-tinggi. Minyak trans meningkatkan nilai indeks aterosklerosis dan indeks resiko koroner mencit.Coronary Heart Disease (CHD) is a "silent killer" disease. Various studies were developed through an animal model approach to studying CHD. Cooking oil that is used repeatedly can increase levels of free fatty acids which have a negative impact on health, especially the heart and vessels. The aim of this study was to measure the plasma atherogenic index (IAP) and Castelli index (IC) of trans oil-induced model mice (MT). A total of 16 DDY mice were divided into 4 groups, namely mice induced with 20% trans oil (MT20), 40% trans oil (MT40) and 60% trans oil (MT60) and control (K). The percentage of trans oil is given based on the total energy consumption of the daily feed. Trans oil is given orally once a day for 70 days. Blood was taken on the 70th day from the lateral vein of the rat tail to measure the plasma lipid profile, namely total cholesterol, triglycerides (TG), High Density Lippoprotein (HDL) and plasma Low Density Lipoprotein (LDL) using the Lipid ProÂ® rapid test kit. The Plasma Atherogenic Index (IAP) values are log (TG / HDL) and the Castelli Index (IC) is LDL / HDL. The results of measuring the plasma lipid profile showed that plasma total cholesterol, triglycerides, HDL and LDL in the MT group increased significantly compared to the control (sig<0,05). The IA value in mice treated with trans oil was significantly different from the control (sig<0.05) and had a high risk. The IC value in mice treated with trans oil was significantly different from the control (sig<0.05) and had a medium-high risk. Trans oil increased the atherosclerosis index and coronary risk index of mice.

Improvements Glutation Levels in the Hippocampus of Aged and Oxidative-Stressed Rats by Supplementation of Alanine-Glutamine Dipeptide Sunarno, Sunarno; Manalu, Wasmen; Kusumorini, Nastiti; Agungpriyono, Dewi Ratih
Sains Medika : Jurnal Kedokteran dan Kesehatan Vol 4, No 1 (2012): Januari-Juni 2012
Publisher : Fakultas Kedokteran; Universitas Islam Sultan Agung (UNISSULA)

Background: Physiological aging or aging due to oxidative stress causes to disruption of glutathione homeostasis of the hippocampus. Disruption of glutathione homeostasis characterized by a decrease in hippocampus glutathione levels below the normal threshold. Decreased levels of glutathione in the hippocampus was demonstrated decreased hippocampus function. One way to increase glutathione levels in the hippocampus and the function of hippocampus is to increase the concentrations of glutathione precursor. One of the glutathione precursors is alanine-glutamine dipeptide. This research was designed to obtain the most effective level of alanine-glutamine dipeptide supplementation to improve glutathione synthesis in the hippocampus of aged and oxidative-stressed rats. The highest glutathione concentrations in the hippocampus showed the best improvement on the hippocampus function in physiological aging or oxidative-stress aging rats.Design and Methods: The experimental rats were assigned into a completely randomized design with 2x2x4 factorial arrangement. The first factor was the age of the experimental rats, consisted of two levels i.e., 12 and 24 months. The second factor was oxidative stress consisted of two levels, i.e., without or with oxidative stress. The third factor was the level of alanine-glutamine dipeptide administration consisted of 4 concentrations, i.e. 0%, 3%, 5%, and 7%.Results: The results showed that administration of 7% alanine-glutamine dipeptide has resulted the highest levels of glutathione hippocampus in younger (0.0154 mg/mg tissue) or aged (0.0140 mg/mg tissue) rats or in normal (0.0150 mg/mg tissue) and in oxidative-stressed (0.0144 mg/mg tissue) rats. The increased hippocampus glutathione levels were associated to the improved functions of the hippocampus. Conclusion: alanine-glutamine dipeptide administration of 7% consentrations gave the best results on repair function of the hippocampus and has the potential to slow aging, both physiological aging or oxidative-stress aging rats (Sains Medika, 4(1):1-12).

PENGAMATAN AKTIVITAS TIKUS WISTAR JANTAN DENGAN ALAT OPTO- VARIMEXÂ® PADA KONDISI DIET TINGGI MINYAK TRANS MASYITA, NURUL; SANTOSO, KOEKOEH; KUSUMORINI, NASTITI; SATYANINGTIJAS, ARYANI SISMIN; SUPIYANI, ATIN
Bioma Vol. 11 No. 1 (2015): Bioma
Publisher : LPPM Universitas Negeri Jakarta

ABSTRACT Excessive energi consumption of high-fat diet increase the bodyâ€™s adipose tissue which also affect the increased secretion of leptin, this is because leptin secretion is proportional to the mass of adipose tissue. Leptin activates the lateral hypothalamus as the center of hunger and feeding activity. The aim of this study was to obtain the relationship between high trans fat feeding on male Wistar rats and feeding activity. This method uses male Wistar rats (Rattus norvegicus) as an animal model. Sixteen rats were grouped into 4 treatment groups. The first group was given additional feed 20% trans fat, the second group was given additional feed 40% trans fat, a third group was given additional feed 60% trans fat, and the last as a control group. This feed was given once a day for 70 days. Observation of activities perform using Opto-varimexÂ® auto-track system ver. 4.31. The results showed an corellation between activity based doses of trans fat. Keywords: Opto-varimexÂ®, Trans fat, Activity, Wistar rat.

DETERMINASI PEMBERIAN MINYAK TRANS TERHADAP STRES OKSIDATIF PADA TIKUS GALUR WISTAR DENGAN MALONDIALDEHIDA PANKREAS SEBAGAI INDIKATOR Mawali, Maharja; Santoso, Koekoeh; Kusumorini, Nastiti; Satyaningtijas, Aryani Sismin; Supiyani, Atin
Bioma Vol. 11 No. 2 (2015): Bioma
Publisher : LPPM Universitas Negeri Jakarta

ABSTRACT Trans fatty acids (TFAs) are the entire unsaturated fatty acids containing one or more double bonds isolated in trans configuration believed as predisposing factor of oxidative stress, excluding conjugated double bond fatty acids. Malondialdehyde (MDA) is a product of lipid peroxidation used as oxidative stress marker. The objective of this study was to see the correlation between TFAs containing-oil (consists of 3-6% TFAs) intake at various administration doses and pancreatic MDA levels in Wistar rats. Twelve rats were grouped according to administration doses (20%, 40%, 60% of given feed total energy and control group). TFAs containing-oil was administered orally once a day for 70 days at given doses by force feeding. Pancreatic MDA were measured using TBARS assay adapted from Singh et al. (2006). Increase of TFAs containing-oil administration dose caused insignificant increase of pancreatic MDA level. Keywords: trans fatty acids, oxidative stress, malondialdehyde, Wistar rats, palm oil

DETERMINASI PEMBERIAN SUKROSA TERHADAP KADAR SGPT DAN SGOT TIKUS GALUR WISTAR SEBAGAI INDIKATOR FUNGSI HATI: Determination of High Sucrose Intake to SGPT and SGOT Concentration in Wistar Rats As Indicator of Liver Function. Andayani, Prawita Lintang; Santoso, Koekoeh; Kusumorini, Nastiti; Satyaningtijas, Aryani Sismin; Supiyani, Atin
Bioma Vol. 12 No. 1 (2016): Bioma
Publisher : LPPM Universitas Negeri Jakarta

The aim of the study was to determine the correlation between sucrose intake at various administration doses to SGPT and SGOT level in Wistar rats. SGPT and SGOT level in blood serum were used as parameter of liver function. Twelve rats were grouped according to administration doses (20%, 40%, 60% of given feed total energy and control group). Sucrose was administered orally once a day for 70 days at given doses by force feeding. SGPT dan SGOT level were measured using SGPT and SGOT Test Kit and read using spectrophotometer. The result indicated that increased administration dose caused a significant increase of SGPT and SGOT level.

PERUBAHAN KUALITAS SPERMATOZOA DAN JUMLAH SEL-SEL SPERMATOGENIK TIKUS YANG TERPAPAR ASAP ROKOK Jems Akiles Unitly, Adrien; Kusumorini, Nastiti; Agungpriyono, Srihadi; Sismin Satyaningtijas, Aryani; Boediono, Arief
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i2.2629

Penelitian ini bertujuan mengetahui efek asap rokok terhadap perubahan kualitas spermatozoa dan jumlah sel-sel spermatogenik. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan perlakuan asap rokok 10 batang/ekor/hari selama 2,5 jam dalam smoking chamber terhadap 24 ekor tikus jantan yang dibagi menjadi 4 kelompok, masing-masing 6 ekor dalam tiap kelompok. Kelompok P1 adalah kelompok yang tidak diberi perlakuan (kontrol), P2 adalah kelompok yang dipapar asap rokok selama 20 hari, P3 adalah kelompok yang dipapar asap rokok selama 40 hari, P4 adalah kelompok yang dipapar asap rokok selama 60 hari. Parameter yang diamati adalah kualitas spermatozoa dan jumlah sel-sel spermatogenik. Hasil penelitian menunjukkan bahwa pemaparan asap rokok dapat menurunkan konsentrasi spermatozoa dan viabilitas spermatozoa serta meningkatkan abnormalitas spermatozoa. Disimpulkan bahwa pemaparan asap rokok 10 batang/ekor/hari menyebabkan penurunan kualitas spermatozoa dan jumlah sel-sel spermatogenik yang tidak kembali ke kondisi normal setelah proses penyembuhan.

PENURUNAN KADAR PROGESTERON SERUM DAN KOMPONEN MATRIKS EKSTRASELULER DAN SELULER KULIT SEBAGAI INDIKATOR PENUAAN PADA TIKUS s, Safrida; Kusumorini, Nastiti; Manalu, Wasmen; Maheshwari, Hera
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i1.557

Penelitian ini bertujuan mengetahui kadar kolagen kulit (matriks ekstraseluler), jumlah sel (kadar DNA), aktivitas sintetik (kadar RNA) padajaringan kulit, serta kadar hormon progesteron pada berbagai tingkatan umur tikus, dan memperoleh umur tikus ovariektomi yang cocok digunakan sebagai hewan model penuaan. Rancangan percobaan yang digunakan adalah rancangan acak lengkap (RAL) yang terdiri atas sembilan kelompok percobaan masing-masing terdiri atas tiga ekor tikus, yaitu umur 12 bulan (K1), 18 bulan (K2), 24 bulan (K3), 30 bulan (K4), 36 bulan (K5), umur 12 bulan dalam kondisi 1 bulan pascaovariektomi (K6), 12 bulan dalam kondisi 3 bulan pascaovariektomi (K7 ), 18 bulan dalam kondisi 1 bulan pascaovariektomi (K8), dan umur 24 bulan dalam kondisi 1 bulan pascaovariektomi (K9). Data yang diperoleh dianalisis dengan menggunakan analisis varian dan dilanjutkan dengan uji Duncan. Komponen matriks ekstraseluler dan matriks seluler menurun seiring dengan bertambahnya usia. Umur tikus normal yang cocok digunakan sebagai hewan model penuaan adalah tikus umur 24-36 bulan. Tikus ovariektomi yang cocok digunakan sebagai hewan model penuaan adalah tikus umur 12 bulan pascaovariektomi 3 bulan.

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