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All Journal Majalah Obat Tradisional JOURNAL OF COMMUNITY ENGAGEMENT AND EMPOWERMENT Jurnal Sintesis: Penelitian Sains, Terapan dan Analisisnya Jurnal Ilmiah Manuntung: Sains Farmasi dan Kesehatan
Tri Puji Lestari
Institut Ilmu Kesehatan Bhakti Wiyata Kediri
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Veterinary Other

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Physical Characteristic and Antibacterial Activity of Silver Nanoparticles from Green Synthesis Using Ethanol Extracts of Phaleria macrocarpa (Scheff.) Boerl Leaves Tri Puji Lestari; Fitia Abbas Tahlib; Johan Sukweenadhi; Kartini kartini; Christina Avanti
Majalah Obat Tradisional Vol 24, No 1 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4875.435 KB) | DOI: 10.22146/mot.37956

Abstract

The Green Synthesis method is widely developed due to its environmentally friendly, cost-effective, and easy application for nanoparticle-scale synthesis. Among all metal nanoparticles, silver nanoparticles are the most utilized products in the field of nanotechnology. Biomolecules contained in plant extracts can reduce the size of silver particles to nano size. This study aims to determine the antibacterial properties and activity of silver nanoparticles synthesized with the ethanol extract of mahkota dewa leaves. The formation of silver nanoparticles is monitored with UV-Vis absorption and its change in color. Parameters evaluated are shape, size, particle size distribution, composition, metal residue, and a functional group of nanoparticles, using Scanning Electron Microscopy (SEM), Particle Size Analyzer (PSA), X-Ray Diffraction (XRD), and Fourier Transform InfraRed (FTIR) instrument. The research on UV-Vis color and absorption show black silver color with the wavelength of 450-465nm. The characterization result shows spherical-shaped silver nanoparticle. Furthermore, PDI best value on concentration of 0.125% is 0,221±0,0482 with average particle size of 130,300±12,6858 nm. The diffraction pattern of silver nanoparticle with XRD test indicates that the nanoparticles contain the silver component. Antibacterial activity test shows that silver nanoparticles have a greater inhibition zone than AgNO3, and 0.125% ethanol extract of mahkota dewa leaves against Escherichia coli and Staphylococcus aureus. From the results of the study, it can be concluded that the ethanol extract from mahkota dewa leaves can be used as a bioreductor agent to produce silver nanoparticles which have greater antimicrobial activity compared to Ag and ethanol extract from mahkota dewa leaves.
Pengaruh HPMC Sebagai Gelling Agent Pada Optimasi Formula Gel Ekstrak Kasar Bromelin Kulit Nanas ( Ananas comossus L. Merr): - Anang Setyo Wiyono; Tri Puji Lestari; Very Setya Wardani
Jurnal Sintesis: Penelitian Sains, Terapan dan Analisisnya Vol 1 No 2 (2020): Desember 2020
Publisher : Fakultas Sains, Teknologi, dan Analsisi Institut ilmu Kesehatan Bhakti Wiyata

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (469.834 KB)

Abstract

Nanas (Ananas comosus (L.) Merr) merupakan salah satu jenis buah yang diminati oleh masyarakat, baik lokal maupun dunia. Pemanfaatan kulit nanas diketahui belum optimal sehingga penelitian ini menggunakan kulit nanas sebagai bahan aktif gel. Kulit nanas memiliki senyawa fitokimia yang khas yaitu bromelin yang dapat digunakan sebagai antimemar. Bromelin tergolong kelompok enzim protease sulfhidril yang mampu menguraikan struktur molekul protein menjadi asam - asam amino. Ekstrak kasar bromelin kulit nanas diperoleh dari ekstraksi menggunakan metode Herdyastuti (2006) kemudian dimasukkan ke dalam formulasi sediaan gel. Terdapat 3 variasi formulasi gel hasil Simplex Lattice Design dengan perbandingan konsentrasi HPMC : gliserin, yaitu FA(0% : 100%), FB (50% : 50%), FC (100% : 0%). Ketiga variasi formulasi dioptimasi berdasarkan hasil uji pH, daya sebar, dan viskositas untuk mendapatkan formulasi optimum. Formulasi optimum yang diperoleh yaitu konsentrasi HPMC : gliserin sebesar 30% : 70%. Gel hasil formulasi optimum kemudian dianalisis menggunakan uji T dan diketahui tidak terdapat perbedaan yang bermakna antara hasil prediksi dan kenyataan dilihat dari karakteristik gel meliputi pH, daya sebar, dan viskositas.
Sosialisasi dan Pelatihan Pembuatan Hand Body Lotion Dengan Memanfaatkan Lidah Buaya Untuk Pelembab Kulit Ida Kristianingsih; Evi Kurniawati; Tri Puji Lestari
Journal of Community Engagement and Empowerment Vol 4, No 1 (2022)
Publisher : Institut Ilmu Kesehatah Bhakti Wiyata Kediri

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Abstract

Permasalahan tentang perawatan kecantikan ini terjadi pada Santriwati yang ada di Pondok pesantren Trisakti. Mereka tidak cukup waktu untuk harus berlama-lama di klinik kecantikan. Kulit kering akibat kurang kelembaban menjadi permasalahan di antara mereka. Faktor lingkungan yang buruk dan kondisi stress dapat merusak kesehatan kulit, termasuk faktor alami yang berasal dari dalam tubuh seperti pertambahan usia. Salah satu upaya untuk menjaga kecantikan dan kesehatan kulit dengan menggunakan hand body lotion. Fungsi utama dari pelembab, khususnya hand body lotion adalah menjaga kelembaban kulit. eperti diketahui mandi dan memakai sabun dapat menghilangkan kelembaban alami kulit. Pemakaian hand body lotion setelah mandi akan mengganti kelembaban yang hilang sekaligus membantu menjaga kelembabankulit sepanjang hari. Tujuan : Pengabdian masyarakat ini bertujuan untuk memberikan edukasi remaja Putri di pondok pesantren Tri Sakti tentang cara pembuatan hand body lotion dengan memanfaatkan lidah buaya. Saat ini sangat penting untuk seorang Wanita bisa merawat dan memelihara kesehatan kulit mereka dengan memanfaatkan bahan alam yang ada disekitar mereka. Metode: Kegiatan yang digunakan untuk pengabdian ini dimulai dengan pemberian kuisioner yang digunakan sebagai pretest kepada para peserta, dilanjutkan dengan presentasi oleh pemateri, demontrasi cara pembuatan hand sanitizer dan di akhiri dengan post test. Hasil: Kegiatan ini memberiakan dampak yang positif terlihat terlihat dari antusiasme peserta dan terlihat dari peningkatan nilai hasil post test. Kegiatan ini bisa memberikan dampak ekonomi dengan mengurangi biaya pengeluaran untuk kosmetik.
AKTIVITAS ANTIBAKTERI DARI SEDIAAN GEL EKSTRAK TERPURIFIKASI KELOPAK ROSELLA TERHADAP BAKTERI Escherichia coli Fita Sari; Ida Kristianingsih; Tri Puji Lestari; Fathul Hidayatul; Yuyun Wulandari
Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan Vol. 9 No. 1 (2023): Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v9i1.674

Abstract

Natural materials had various types of primary and secondary metabolite compounds that were useful for treating a disease. Plant materials that contain high levels of secondary metabolites could be developed into raw materials for traditional medicines. An example of the development of natural medicine which was in high demand is making traditional medicinal raw materials which have antibacterial properties. These could add to the type of therapy for a disease with smaller side effects. The aim of this study was to determine the activity of the purified extract gel preparation of rosella petals as an antibacterial. The extract was obtained from the maceration method which was then followed by purification. The antibacterial activity test in this study used the well diffusion method. Detection of metabolites by Thin Layer Chromatography (TLC) includes the stationary phase of silica GF254 and the mobile phase Toluene: Acetic acid: Formic acid (6:6:1). The concentration of purified extract of rosella petals for the formulation was 5, 15 and 25%. Data analysis was carried out with the One Way Anova test with a 95% confidence level. The results of the research that the purified extract gel preparation of rosella petals has the potential to inhibit the growth of Escherichia coli bacteria in the sensitive category at a concentration of 25%. The results of a positive phytochemical screening for flavonoid compounds were indicated by the formation of a red-orange color in the test and identification using TLC produced an Rf of 0.85 which has similarities with previous studies. The conclusion of this study was that the ETKR gel preparation with a concentration of 25% was sensitive as an antibacterial
VALIDASI METODE ANALISIS KUERSETIN DARI EKSTRAK ETANOL DAUN KEMANGI (Ocimum sanctum L) SECARA SPEKTROFOTOMETRI UV-Vis Evi Kurniawati; Tri Puji Lestari; Krisna Kharisma Pertiwi
Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan Vol. 10 No. 1 (2024): Jurnal Ilmiah Manuntung: Sains Farmasi Dan Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51352/jim.v10i1.780

Abstract

Basil leaves, scientifically known as Ocimum sanctum L., are a medicinal plant commonly used by the public because of their abundant secondary metabolite chemical compounds. One of them is flavonoids, secondary metabolites found in basil leaves, which have antioxidant properties. Quercetin, a flavonoid compound, exhibits valuable biological properties beyond its antioxidant effects, including antibacterial, anticancer, antiviral and anti-inflammatory activities. This research was carried out with the aim of determining the quercetin content contained in the ethanol extract of basil leaves (Ocimum sanctum L) by validated UV-Vis Spectrophotometry. Basil leaves are macerated with 70% ethanol. The extract is then concentrated using a rotary evaporator. Next, phytochemical screening was carried out to identify secondary metabolites in the extract, followed by UV-Vis spectrophotometric quantification. Based on the test results, it can be seen that the analysis of quercetin levels using UV-Vis spectrophotometry is validated for all parameters that meet the requirements. A significant relationship is shown by the linearity test, the correlation coefficient (r) is 0.9963. These results show good specificity, with test accuracy ranging from 90% to 107%, namely 103.9%. In addition, the precision test shows the %RSD value is less than 2%. The ethanol extract of basil leaves has an average quercetin content of 10.811% w/w.
Co-Authors Anang Setyo Wiyono Christina Avanti Fathul Hidayatul Fitia Abbas Tahlib Ida Kristianingsih Kartini kartini KRISNA KHARISMA PERTIWI Kurniawati, Evi Sari, Fita Very Setya Wardani Yuyun Wulandari