Garuda - Garba Rujukan Digital

p-Index From 2021 - 2026

0.947

P-Index

This Author published in this journals

All Journal CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Jurnal Kimia (Journal of Chemistry) Itepa : Jurnal Ilmu dan Teknologi Pangan Jurnal Media Sains

Anak Agung Istri Agung Mayun Laksmiwati

Progam Studi Kimia Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Udayana

Author-ID : 304285

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Electrical & Electronics Engineering Immunology & microbiology Medicine & Pharmacology

Published : 28 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3

KADAR THIAMIN HIDROKLORIDA (VITAMIN B1) PADA NASI BERAS PUTIH DAN BERAS MERAH PADA BERBAGAI WAKTU PENYIMPANAN PADA ALAT MAGIC-COM A. A. I. A. Mayun Laksmiwati; Ketut Ratnayani; Ni Wayan Agustini
Jurnal Kimia (Journal of Chemistry) Vol. 6, No. 1 Januari 2012
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.08 KB)

This paper discusses the influence of storage time on the thiamine hydrochloride content in rice. White rice (C36) and red rice were stored in a magic-com for a period of time, of 0 hours, 6 hours and 12 hours. Thiamine hydrochloride contents in rice were separated with the use of a chromatography column with alumina oxide, while and the measurement was carried out by UV-Vis spectrophotometry.It was found that the thiamine hydrochloride content in red rice stored within 0 hours, 6 hours and 12 hours were, 5.3 mg/kg, 4.5 mg/kg and 2.8 mg/kg, respectively, while in white rice they were found to be 2.6 mg/kg, 2.0 mg/kg and 1.4 mg/kg, respectively.

UJI AKTIVITAS PROTEASE GETAH LABU SIAM DAN TALAS SERTA PERBANDINGANNYA TERHADAP GETAH PEPAYA Ketut Ratnayani; A. A. Ayu Septri Juwarni; A. A. I. A. Mayun Laksmiwati; I G. A. Kunti Sri Panca Dewi
Jurnal Kimia (Journal of Chemistry) Vol. 9, No. 2 Juli 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Papaya (Carica papaya L.) latex has been used commercially as a protease (papain) sources. However it can cause itchy on the skin. Some other latex plants that can cause itchy were taro and chayote. They were strongly predicted to have protease components. This research aimed to determine protease activity of plants latex of taro (Xantosoma sagittifolium (L.) Schott and chayote (Sechium edule (Jacq.) Sw.). The protease activity of these latex then were compared to the protease activity of papaya latex. Latex of papaya and taro were collected from fruit, while latex of chayote was collected from corm. The assay of protease activity was based on spectrofotomeric methods (Anson’s method) using casein as substrat. The result of protease activity assay showed that taro and chayote latex positively contains protease but their protease activities were less than papaya latex which were 0.0123 U/mL and 0.0264 U/mL respectively. It can be concluded that taro and chayote latex were potentially used as alternative protease sources. The protease activity ratio of taro latex to papaya was 1: 74.75 and the ratio of chayote latex to papaya latex was 1: 34.82.

AKTIVITAS PROTEASE PADA GETAH BAGIAN BATANG DARI TIGA JENIS SPESIES TANAMAN KAMBOJA (Plumeria L) K. Ratnayani; M. Nazib; J. Sibarani; A.A.I.A M. Laksmiwati
Jurnal Kimia (Journal of Chemistry) Vol.12 No.2 Juli 2018
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Penelitian ini bertujuan untuk melakukan uji aktivitas protease pada getah bagian batang dari tiga spesies tanaman kamboja yaitu Plumeria rubra L, Plumeria obtusa L dan Plumeria pudica Jacq yang banyak dibudidayakan di wilayah Bali. Pengujian aktivitas protease dilakukan secara spektrofotometri (metode Kurnitz termodifikasi) menggunakan kasein sebagai substrat. Hasil penentuan aktivitas protease pada tiga spesies tanaman kamboja menunjukkan bahwa spesies Plumeria rubra L. memiliki aktivitas protease rata-rata relatif lebih tinggi yaitu 0,0398 ± 0,00034 U/mL, disusul oleh spesies Plumeria pudica Jacq. yaitu 0,0371 ± 0,00019 U/mL, dan Plumeria obtusa L. yaitu 0,0365 ± 0,00032 U/mL. Spesies Plumeria rubra L. merupakan jenis tanaman kamboja yang paling umum, memiliki paling banyak varietas dan paling banyak dibudidayakan di wilayah Bali dibandingkan spesies lainnya. Dari hasil penelitian ini dapat diketahui bahwa jenis ini berpotensi sebagai sumber protease alternatif. Kata kunci: aktivitas protease, getah kamboja, tiga spesies

PENGARUH EKSTRAKSI TERHADAP EFEKTIVITAS SERBUK BIJI SEMANGKA (CURCUBITACEAE) DAN SERBUK BIJI ASEM (FABACEAE) SEBAGAI KOAGULAN ALAMI PENGGANTI TAWAS - Yunita; Anak Agung Istri Agung Mayun Laksmiwati; Ni Komang Ariati
Jurnal Kimia (Journal of Chemistry) Vol. 11. No. 1 Januari 2017
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Koagulan alami adalah koagulan yang berasal dari tanaman biji yang mengandung protein polikationik sehingga dapat menetralisir partikel muatan dalam rantai koloid. Protein yang larut dalam air akan bermuatan positif, dan memiliki sifat polielektrolit yang sama dengan tawas. Penelitian ini menggunakan serbuk biji asem dan semangka sebagai koagulan alami, dengan membandingkan hasil filtrat per hari yang dimaserasi dengan n-heksan, etanol, tanpa maserasi serta residu hasil maserasi. Pengukuran tingkat kekeruhan dilakukan dengan menggunakan metode jar test. Sedangkan penurunan kekeruhan dilakukan dengan alat turbidimeter. Hasil penelitian menunjukkan bahwa penurunan kekeruhan (NTU) filtrat serbuk biji asem yang dimaserasi n-heksan terbaik di hari keenam dan yang dimaserasi etanol terbaik di hari pertama dengan NTU berurutan 72,16% dan 82,16%, sedangkan yang tanpa maserasi NTUnya 60,00%. Pada filtrat serbuk biji semangka yang dimaserasi n-heksan terbaik di hari ketiga dan yang dimaserasi etanol terbaik di hari kelima dengan NTU 69,75% dan 65,50%, sedangkan yang tanpa maserasi NTUnya 52,50%. Nilai tingkat kekeruhan dari residu serbuk biji asem yang dimaserasi dengan n-heksan dan etanol berturut-turut 70,16% dan 62,00%, sedangkan NTU dari residu biji semangka yang dimaserasi dengan n-heksan dan etanol menunjukkan hasil yang sama yaitu 60,00%. Dari hasil penelitian dapat dikatakan bahwa filtrat dari serbuk biji asem yang dimaserasi etanol paling baik sebagai koagulan alami pengganti tawas, dibandingkan filtrat serbuk biji semangka dan residunya.

PENGARUH WAKTU INKUBASI TERHADAP AKTIVITAS LIPASE YANG DIINDUKSI DENGAN MINYAK JELANTAH PADA TANAH DARI HUTAN MANGROVE PANTAI SUWUNG KAUH BALI A A I A Mayun Laksmiwati; I Nengah Wirajana; Diah Suci
Jurnal Kimia (Journal of Chemistry) Vol. 10, No. 2 Juli 2016
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Tanah hutan mangrove Pantai Suwung Kauh Bali dimanfaatkan sebagai sumber penghasil enzim, salah satunya adalah lipase. Penelitian ini bertujuan untuk mengetahui besarnya aktivitas lipase dengan dan tanpa penambahan minyak jelantah dan pengaruh waktu inkubasi terhadap aktivitas lipase tanah hutan mangrove. Metode titrasi asam-basa digunakan dalam pengukuran aktivitas lipase dengan waktu inkubasi selama 0,1,2,3,4,5,6 dan 7 hari. Hasil penelitian menunjukkan bahwa penambahan minyak jelantah dapat meningkatkan aktivitas lipase. Peningkatan aktivitas lipase diduga disebabkan oleh lipida yang terkandung dalam minyak jelantah dapat menginduksi lipase dari mikroorganisme lipolitik yang ada dalam tanah. Aktivitas lipase tertinggi diperoleh sebesar 0,0996 U/mL dengan penambahan minyak jelantah pada inkubasi hari ke-6. Aktivitas lipase dengan penambahan minyak jelantah dan dengan aerasi dihasilkan aktivitas lipase yang lebih tinggi sebesar 0,1250 U/mL dengan waktu inkubasi 5 hari. Waktu inkubasi berpengaruh nyata terhadap aktivitas lipase pada tanah hutan mangrove dengan dan tanpa penambahan minyak jelantah.

KADAR TOTAL SENYAWA FENOLAT PADA MADU RANDU DAN MADU KELENGKENG SERTA UJI AKTIVITAS ANTIRADIKAL BEBAS DENGAN METODE DPPH (Difenilpikril Hidrazil) Ketut Ratnayani; A. A. I. A Mayun Laksmiwati; Ni P. Indah Septian P.
Jurnal Kimia (Journal of Chemistry) Vol. 6, No. 2 Juli 2012
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.645 KB)

Honey contains a variety of compounds which function as antioxidants one of which is a phenolic compound. Monoflora honey used in this study were randu and kelengkeng honey from certain brand distributed on the market. Qualitative test with 5% FeCl3 result showed that both types of honey contain phenolic compounds. The total phenolic compounds was determined by spectrophotometry using the Folin-Ciocalteu method and gallic acid as standards, while the free radical activity was tested using the method of DPPH (Difenilpikril hidrazil). The result of research showed that the total content of phenolic compound in randu was 1375,89 ± 134,10 mg GAE/kg, while kelengkeng honey was 1136,49 ± 39,62 mg GAE/kg. The % reduction of free radical in randu honey at the 5th minute was 62,55 ± 4,4407 % and at the 60th minute was 95,39 ± 8,5507 %. The % reduction of free radical for kelengkeng honey at the 5th minute was 44,12 ± 1,3433 %, 60th minute was 62,00 ± 0,8612 %, and for the standard of gallic acid the % reduction of free radical at the 5th minute was 41,03% and the 60th minute was 92,00%. Therefore, there was a linear correlation between the total phenolic compound of randu honey and kelengkeng honey with % reduction of its free radical.

ANALISIS KADAR ASAM ASKORBAT (VITAMIN C ) PADA MINUMAN SUPLEMEN DALAM KEMASAN DENGAN METODE SPEKTROFOTOMETRI SECARA LANGSUNG DAN TIDAK LANGSUNG I W. Sudiarta; I P. G. A. Suandi; A. A. I. A. M. Laksmiwati
Jurnal Kimia (Journal of Chemistry) Vol. 15, No.2, Juli 2021
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JCHEM.2021.v15.i02.p03

Asam askorbat atau vitamin C banyak terdapat pada minuman suplemen kemasan yang mudah ditemukan dimanapun dan penggunaanya yang relatif lebih praktis. Untuk mengetahui kadar vitamin C yang tepat dalam minuman suplemen dilakukan pengukuran dengan metode spektrofotometri UV-Vis secara langsung dan tidak langsung. Penelitian ini bertujuan untuk mengetahui metode manakah yang lebih baik untuk analisis vitamin C dan mengetahui apakah kadar vitamin C yang tercantum pada label minuman suplemen kemasan sesuai dengan hasil metode langsung dan tidak langsung. Sampel dipreparasi menggunakan metode spektrofotometri secara langsung dan tidak langsung menggunakan logam pereduksi Cr6+ yang direaksikan dengan vitamin C pada sampel dan dianalisis menggunakan spektrofotometer UV-Vis. Hasilnya menunjukkan bahwa analisis vitamin C dengan metode spektrofotometri lebih baik secara tidak langsung daripada secara langsung. Kadar vitamin C pada sampel minuman suplemen dalam kemasan A, B, dan C yang dianalisis dengan metode spektrofotometri secara langsung diperoleh masing-masing sebesar 1074,34 mg/L ; 5956,44 mg/L dan 6646,28 mg/L dengan nilai %kesesuaian kadar 88,91%, 89,35%, dan 89,66%. Sedangkan kadar vitamin C pada sampel minuman suplemen dalam kemasan A, B, dan C yang dianalisis dengan metode spektrofotometri secara tidak langsung diperoleh masing-masing 1178,17 mg/L ; 6162,41 mg/L dan 6718,58 mg/L dengan % kesesuaian kadar 97,50%, 92,44% dan 90,63%. Hasil uji -t menyatakan tidak ada perbedaan secara signifikan antara kadar vitamin C sampel A yang dianalisis dengan metode tidak langsung dengan kadar yang tertera di label kemasan. Sedangkan kadar vitamin C pada sampel B tidak berbeda secara signifikan baik ditentukan dengan metode langsung maupun tidak langsung. Kata kunci: Cr6+, minuman suplemen dalam kemasan, spektrofotometer UV-Vis, Vitamin C. Ascorbic acid or vitamin C contained in many supplement drinks are easily found anywhere and its use is relatively more practical. To find out the exact content of vitamin C in supplement drinks, measurements were carried out directly and indirectly using UV-Vis spectrophotometry method. This study aims to find out which spectrophotometric method is better for vitamin C analysis and whether the vitamin C content listed on the supplement drink packaging label is in accordance with the results of the direct and indirect methods. Samples were prepared directly and indirectly using Cr6+ reducing metals reacted with the vitamin C in the samples and analyzed by using a UV-Vis spectrophotometer methods. The results showed that the analysis of vitamin C by indirect spectrophotometric method was better than the direct one. Vitamin C contents in the supplement beverage samples of packaging A, B, and C analyzed using direct spectrophotometric method were 1074.34 mg/L; 5956.44 mg/L and 6646.28 mg/L with percentage of conformity of 88% 91.91%, 89.35% and 89.66%, respectively. Meanwhile, the vitamin C contents in the supplement drink samples of packaging A, B, and C analyzed by indirect spectrophotometric method were 1178.17 mg/L; 6162.41 mg/L and 6718.58 mg/L with percentage of conformity of 97.50%, 92.44% and 90.63%. The -t test results indicated that there was no significant difference of vitamin C content in the sample A analyzed by indirect methods from the content listed on the packaging label. The -t test results also stated that there is no significant difference of vitamin C content in the sample B analyzed either by direct or indirect method. Keywords: bottled supplement drinks, Cr6+, UV-Vis Spectrophotometer, vitamin C.

ANALISIS VARIASI NUKLEOTIDA DAERAH D-LOOP DNA MITOKONDRIA PADA SATU INDIVIDU SUKU BALI NORMAL Ketut Ratnayani; I Nengah Wirajana; A. A. I. A. M. Laksmiwati
Jurnal Kimia (Journal of Chemistry) Vol. 1, No. 1 Januari 2007
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (459.578 KB)

Human mitochondrial DNA (mtDNA) has higher polimorfism level than nucleous genom, especially in theD-Loop region, which is a non coding region and the most polymorfic region in the mitochondrial genom. Theanalysis of variation of nucleotide sequence of D-Loop region can be used to determine the individual or ethnicidentity and also maternal familiar relationship. The research aims to determine nucleotide variant on Balineseindividue, which can be used as data base in determination of mtDNA genetical profile of Balinese ethnic in a biggerscale.To achieve the aims of the research, way the nucleotide sequence of one normal Balinese individue usingthe epithelia cells in the saliva. The methods were :1) the isolation of sample mtDNA, 2) the amplification of the DLoopregion of mtDNA by PCR, 3) sequencing and analysis of nucleotides sequence.The 0,4 kb fragment of the D-loop region mtDNA of the sample were successfully amplified, andsequenced of 402 pb. The research found 6 new variants or morfe different from Cambridge or Anderson sequence :variant 16223C®T, 16249T®C, 16259C®T, 16278C®T, 16316A®G, 16375C®A. The research also found thedeletion of T nucleotide on position 16362.

PENENTUAN LAJU REAKSI MAKSIMAL (Vmaks) DAN KONSTANTA MICHAELIS-MENTEN (KM) ENZIM LIPASE PANKREAS PADA SUBSTRAT MINYAK KELAPA, MINYAK SAWIT, DAN MINYAK ZAITUN Ketut Ratnayani; A. A. I. A. Mayun Laksmiwati; Maman Sudiarto
Jurnal Kimia (Journal of Chemistry) Vol. 9, no. 1 Januari 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

A main element in the Michaelis-Menten equation is Km, which is typical for a particular enzyme, with a specific substrate at a certain pH and temperature conditions. The aim of this study is determining the difference in the maximum rates (Vmax) and Michaelis-Menten constant (Km) of pancreatic lipase on the coconut oil, palm oil, and olive oil substrates and the most effective hydrolysis by the pancreatic lipases. Km value was calculated by measuring the rate of the catalyzed hydrolysis with various concentrations of pH, temperature, and the optimum incubation time. Before calculating the value of Vmax and Km, the initial rate (v0) was calculated with the titrimetric method. The results showed that Vmax was 2,11 × 10-3 mmol/min on coconut oil substrate; 2,30 × 10-3 mmol/min on palm oil substrates; and 1,60 × 10-3 mmol/minutes on olive oil substrate. While the pancreatic lipase Km values ??were 1,21 × 104 ppm on coconut oil; 2,29 × 104 ppm on palm oil; and 1,60 × 104 ppm on the olive oil. This results suggested the pancreatic lipase catalyzed the hydrolysis was most effective on coconut oil compared with palm oil and olive oil.

FRAKSINASI DAN BIOAVAILABILITAS LOGAM Pb DAN Cr DALAM SEDIMEN DI PELABUHAN BENOA Ni Luh Eka Lusiana Dewi; Emmy Sahara; A. A. I. A Mayun Laksmiwati
Jurnal Kimia (Journal of Chemistry) Vol. 8, No. 1 Januari 2014
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

The fractionation and the bioavailability determination of Pb and Cr in sediment of Benoa Bay have been studied. This study was aimed to determine the metals concentration in their various chemical forms. A 63 µm nylon mesh screen was used for wet and dry sieving the sediment samples. Through fractionation, the bioavailabilities of both metals could be determined. For the total metals determinations, the samples were digested with aqua regia which is the mixture of HCl and HNO3(3:1). Fractionation was done with the application of the four steps sequential extraction technique. All metals measurements were carried out with the use of the technique of Atomic Absorption Spectrophotometry. The total concentrations of Pb in sediments sieved in wet and dry conditions were 18.4852 mg/kg and 23.3974 mg/kg, respectively, whereas the total concentrations of Cr in those of wet and dry conditions were 17.7131 mg/kg and 24.9371 mg/kg, respectively. The fractionations of Pb in sediments sieved in wet and dry conditions showed the same paterns which were as follow: freely, leachable, dan exchangeable (EFLE) fraction < Fe/Mn-oxide fraction < resistant fraction < organic dan sulfide fraction. The fractionation of Cr in wet sieved sediment was as follows: EFLE fraction < resistant fraction < organic and sulfide fraction < Fe/Mn-oxide fraction whereas that of Cr in dry sieved sediment was as follows EFLE< Fe – Mn oxide fraction < resistant fraction < organic and sulfide fraction. The bioavailable and resistant fractions found for Pb in wet sieved sediment were 74.13% and 22.88%, respectively, whereas those of Pb found in dry sieved sediment were 82.84% dan 13.85%, respectively. The bioavailable and resistant fractions found for Cr in wet sieved sediment were 77.59% and 11.23%, respectively, whereas those of Cr in dry sieved sediment were 90.55% and13.21%, respectively.

Co-Authors - Yunita A. A. Ayu Septri Juwarni Amanda Awalia Ramadhani Ariati, N. K. Devi Esteria Hasianna Purba Diah Suci E. Sahara Emmy Sahara Fikriatun Nurhikmawati Helen Helda Prastika I Gusti Ayu Kunti Sri Panca Dewi I Nengah Wirajana I Nyoman Mika Adi Santosa I P. G. A. Suandi I Wayan Sudiarta I. A. Preharsini Kusuma I. A. R. Astiti Asih Ida Bagus Putu Eristya Putra Inten Hardianti Nizar Iryanti Eka Suprihatin Ita Hidayatus S James Sibarani Ketut Ratnayani Listiyanti, N. K. L. M. Nazib Made Arsa Maman Sudiarto Manuntun Manurung N. M. T. Juliasari N. W. Bogoriani Ni G. A. M. Dwi Adhi Suastuti Ni Komang Ariati Ni Luh Eka Lusiana Dewi Ni Luh Made Noviana Dewi Ni Made Puspawati Ni Made Suaniti Ni P. Indah Septian P. Ni Wayan Agustini Putu Setya Sri Antary Putu Suarya Riyadi, T. W. D. Sulihingtyas Yulius Leo Adeputra

Title

Found 28 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 28 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 28 Documents
Search