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All Journal CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Jurnal Kimia (Journal of Chemistry) Buletin Udayana Mengabdi Bumi Lestari Ecotrophic, Journal of Environmental Science JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health)

I Gusti Ayu Kunti Sri Panca Dewi

Jurusan Kimia Fakultas Metematika Dan Pengetahuan Alam (MIPA) Universitas Udayana

Author-ID : 252594

Agriculture, Biological Sciences & Forestry Humanities Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Environmental Science Public Health

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VALIDASI METODE UJI MERKURI MENGGUNAKAN INDUCTIVELY COUPLED PLASMA EMISSION SPECTROMETRY (ICPE) 9000 Ni Nyoman Trisnawati; I Gst. Ayu Kunti Sri Panca Dewi; Putu Primantari Vikana Suari; Ni Putu Ayu Krismayanti
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 9 No 1 (2021): Cakra Kimia (Indonesian E-Journal of Applied Chemistry)
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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ABSTRAK: UPT Laboratorium Analitik merupakan laboratorium pendidikan yang berfungsi sebagai penunjang kegiatan Tri Dharma Perguruan Tinggi yaitu memberikan praktikum kepada mahasiswa, praktek kerja lapangan, membantu uji kimia dalam penelitian baik mahasiswa maupun dosen dan melayani masyarakat untuk uji kimia dan fisika pada bahan-bahan baik air, limbah, makanan, tanah, kompos dan lain lain. Salah satu parameter yang di uji oleh pelanggan baik mahasiswa, dosen maupun dari masyarakat adalah logam merkuri (Hg). Logam tersebut merupakan salah satu logam yang sangat berbahaya dan beracun sehingga sangat membahayakan kesehatan. Metoda uji logam Merkuri dilakukan dengan menggunakan instrument ICPE 9000 dengan larutan standar Merkuri (Hg). Larutan standar tersebut belum CRM sehingga perlu dilakukan validasi standar. Validasi standar digunakan untuk membuktikan bahwa larutan standar yang digunakan dalam metoda uji memenuhi persyaratan sehingga diperoleh data yang akurat dan dapat dipercaya. Metode yang digunakan dalam penelitian ini adalah validasi metode yang dibagi menjadi 5 validasi metode yaitu uji presisi, uji akurasi, uji LOD, LOQ, dan uji linearitas. Hasil yang diperoleh pada penelitian ini adalah nilai r sebesar 0,9969, nilai LOD dan LOQ sebesar 0,1 mg/L dan 0,3 mg/L, dan sudah terkonfirmasi, hasil uji akurasi sudah masuk dalam rentang yang ditetapkan yakni 90 - 110%, hasil analisis uji presisi memiliki nilai % RSD < 2/3 CV Horwitz. Hasil yang diperoleh sudah memenuhi persyaratan sehingga standar merkuri dengan HgSO4 sudah valid sebagai standar Hg sehingga dapat digunakan dalam metode uji. ABSTRACT: The UPT. Analytical Laboratory of Udayana University is located in Bukit Jimbaran Campus the Laboratory work follows the three responsibilities of Higher Education in Indonesia, namely teaching, research and social services. The laboratory serves academic staff, students of Udayana University and clients outside Udayana University in the area of chemical and physical analysis, research and training. The Laboratory also serves thesociety through providing analysis of waters, waste water, food products, soil, compost, and others. One parmeter that often tested in this laboratory by academic staffs, students and other clients is Mercury (Hg). ICPE 9000 (Inductively Coupled Plasma Emission) Spectroscopyis used in the determining mercury in this laboratory. To calibrate the instrument, certified standard solutions are usually used but because CRM is difficult to find then standard solution is made in the laboratory using HgSO4 Pro Analysis grade.Validation methods in this study consisted of 6 tests namely precision, accuracy, LOD, LOQ and Linearty tests. The results of the five tests were: r value was 0,9969; the value of LOD and LOQ were 0,10 mg/L and 0,30 mg/L respectively and they were already confirmed: result of accuracy was in the range of 90-100 %. Result of precision test was % RSD < 2/3 CV Hortwitz. From the above results it could be concluded that the standard solutions of Hg made from HgSO4 were valid and could be used for Hg analysis with a reliable result. From the above results it can be concluded that the standard solutions made in the laboratory using HgSO4 was valid to use in determination of mercury (Hg) and a reliable resesult was obtained

UJI AKTIVITAS PROTEASE GETAH LABU SIAM DAN TALAS SERTA PERBANDINGANNYA TERHADAP GETAH PEPAYA Ketut Ratnayani; A. A. Ayu Septri Juwarni; A. A. I. A. Mayun Laksmiwati; I G. A. Kunti Sri Panca Dewi
Jurnal Kimia (Journal of Chemistry) Vol. 9, No. 2 Juli 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Papaya (Carica papaya L.) latex has been used commercially as a protease (papain) sources. However it can cause itchy on the skin. Some other latex plants that can cause itchy were taro and chayote. They were strongly predicted to have protease components. This research aimed to determine protease activity of plants latex of taro (Xantosoma sagittifolium (L.) Schott and chayote (Sechium edule (Jacq.) Sw.). The protease activity of these latex then were compared to the protease activity of papaya latex. Latex of papaya and taro were collected from fruit, while latex of chayote was collected from corm. The assay of protease activity was based on spectrofotomeric methods (Anson’s method) using casein as substrat. The result of protease activity assay showed that taro and chayote latex positively contains protease but their protease activities were less than papaya latex which were 0.0123 U/mL and 0.0264 U/mL respectively. It can be concluded that taro and chayote latex were potentially used as alternative protease sources. The protease activity ratio of taro latex to papaya was 1: 74.75 and the ratio of chayote latex to papaya latex was 1: 34.82.

PEMBERIAN EKSTRAK DAUN SIRSAK (Annona muricuta) UNTUK MEMPERBAIKI KERUSAKAN SEL BETA PANKREAS MELALUI PENURUNAN KADAR GLUKOSA DARAH, ADVANCED GLYCATION AND PRODUCT DAN 8-HIDROKSI-2-DIOKSIGUANOSIN PADA TIKUS WISTAR HIPERGLIKEMIA Ni G. A. M. Dwi Adhi Suastuti; I G. A. Kunti Sri Panca Dewi; Ni Komang Ariati
Jurnal Kimia (Journal of Chemistry) Vol. 9, No. 2 Juli 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

This research aimed to determine the effectivity of soursop leaf extract to repair beta pancreatic cells damaged in Wistar rats induced by alloxan. This experiment used randomized posttest-only control group design. In this experiment, 24 Wistar rats were used which divided into four groups, control group (K), and three treatment groups (P). P1 was the group tretment with a dose of 50 mg/kg bw/day, P2 with a dose of 100 mg/kg bw/day, and P3 was the group treatment with a dose of 150 mg/kg bw/day extract. All groups were induced by alloxan with a dose of 150 mg/kg bw to obtain condition of hyperglycemia. The results showed that blood glucose levels decreased after treatment with the extracts. Glucose levels in group K, P1, P2, and P3 were 208.0 mg/dL; 177.5mg/dL; 164.7 mg/L and 137.0 mg/dL respectively. After 2 weeks of treatment, the level of 8-OHdG and AGEs were also reduced. The lowest level was observed in P3 treatment which the level of 8-OHdG 1.664ng.mL and 0.033 mol/L for AGEs level. In conclusion the soursop leaf (Annona muricata) extract given at a dose of 150 mg/kg bw/day can improve beta pancreatic cells resulted from antioxidant activity content in the extract which function as antihyperglycemia. Based on GC-MS spectra analysis, the the soursop leaf (Annona muricata) extract consisted of caryophyllene, ethyl tetradekanoat, acid 2-cyclohexane-1-one, 4-hydroxy-3,5,6-trimethyl-4-(3-oxo-1-butenyl), hexa ethyl decanoate , phytol, thiazole-2,4 (3H, 5H) _dion, 5 benzilideno-3 [etifenilamino) metal], 9-octadecanoic acid, ethyl linoteat, ethyl oleate and ethyl octadecanoic.

SPESIASI DAN BIOAVAILABILITAS LOGAM TEMBAGA (Cu) PADA BERBAGAI UKURAN PARTIKEL SEDIMEN DI KAWASAN PANTAI SANUR Kristina Indra Yanthy T; Emmy Sahara; I G. A. Kunti Sri Panca Dewi
Jurnal Kimia (Journal of Chemistry) Vol. 7, No. 2 Juli 2013
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

A research on bioavailability and speciation of Cu in various sediment particle sizes collected from three sampling sites namely, Sanur, Sindhu and Mertasari Beaches along Sanur Beach was carried out. This study was aimed to determine the fractions of Cu available for aquatic organism and the geochemical forms of Cu in the sediments. Samples pretreatment were carried out by digestion with the use of the mixture of HNO3 and HCl (3:1). The bioavailability and speciation were accomplished by the appliation of single exraction (with EDTA and HCl ) and sequential extraction techniques. Generally, it was found that EDTA extraction yielded higher bioavailability than that of HCl extraction. The highest bioavailability of Cu was showed by the sediment from Sanur Beach and Sindhu Beach at particle size of < 63 µm and from Mertasari Beach at particle size of 63-106 µm. From Cu speciation results it was found that the bioavailable (fractions of easily, freely, leachable, and exchangeable (EFLE), Fe/Mn oxide, organic and sulphide) and the resistant fractions were as follows: Sanur Beach at particle size of <63 µm were 8,28% and 10,31%, particle size of 63-106 µm were 5,34% and 9,13%, and particle size of >106 µm were 18,07% and 5,26%. Sindhu Beach at particle size of <63 µm were 17,32% and 15,69%, particle size of 63-106 µm were 6,98% and 9,92%, and particle size of >106 µm were 7,28% and 7,77%. Mertasari Beach at particle size of <63 µm 8,85% and 11,79%, particle size of 63-106 µm were 13,33% and 20,85%, and particle size of >106 µm were 17,99% and 38,42%.

SPESIASI DAN BIOAVAILABILITAS LOGAM Cu DAN Zn DALAM SEDIMEN DI PELABUHAN BENOA YANG DIAYAK BASAH DAN KERING Arik Agustina; Emmy Sahara; I G.A. Kunti Sri Panca Dewi
Jurnal Kimia (Journal of Chemistry) Vol. 8, No. 1 Januari 2014
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

This paper discusses the speciation and bioavailability of Cu and Zn in sediments of Benoa Bay during which the sediments were sieved in wet and dry conditions. The objective of the study was to determine the concentrations of Cu and Zn in various geochemical forms, therefore the bioavalabilities of the metals could be determined. The determination of the total concentrations of Cu and Zn in the sediments were carried out by the application of extraction with the mixture of HCl and HNO3 (3:1). The speciations of the metals were accomplished with the sequential extraction procedure. The metals concentration measurements were performed by Atomic Absorption Spectroscopy technique with the calibration curve method. It was found that the total Cu concentrations in sediment sieved in wet and dry conditions were 178.4144 mg/kg and 179.9797 mg/kg, respectively. The speciation results for Cu in sediment sieved in wet and dry conditions were as follow: freely, leachable and exchangeable (EFLE) fraction 0.79 and 0.49%, Fe / Mn-oxide fraction 67.75% and 58.71%; organic and sulfide fractions 24.34% and 34.71%, while resistant fraction 2.89% and 2.82%. Therefore, the bioavalable and resistant fractions of Cu in wet sieved sediment were 92,88 % and 2.89%, respectively, while those of dried sieved were 93,92 % and 2,82%, respectively. The total Zn concentrations in sediment sieved in wet and dry conditions were 195.4015 mg/kg and 196.9482 mg/kg, respectively. The speciation results for Zn in sediment sieved in wet and dry conditions were as follow: EFLE fraction 18.83% and 17.40%, Fe / Mn-oxide fraction 35.86 and 37.65%; organic and sulfide fractions 17.16% and 31.09%, while resistant fraction 5.76% and 4.09%. Therefore, the bioavalable and resistant fractions of Cu in wet sieved sediment were 71.85% and 5.70%, respectively, while those of dried sieved were 86.14% and 4.09%, respectively.

SPESIASI DAN BIOAVAILABILITAS LOGAM Cu DAN Zn DALAM PERAIRAN DAN SEDIMEN MUARA SUNGAI BADUNG PADA JALUR TAMAN HUTAN RAYA NGURAH RAI DENPASAR BALI Dewa Ayu Puspasari; Iryanti Eka Suprihatin; I G. A. Kunti Sri Panca Dewi
Jurnal Kimia (Journal of Chemistry) Vol. 8, No. 2 Juli 2014
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Metal concentration in water enviroment and sediment cannot give sufficient information about bioavailability of those metals in the waterworks. The aims of this study are to determine the bioavailability and specification of Cu and Zn in water enviroment and sediment in the estuary of Badung river in the “Taman Hutan Raya Ngurah Rai” Denpasar, Bali area. Determination of bioavailability and speciation of those metals were accomplished by sequencial extraction method. The results of this study show that total concentrations of Cu, and Zn in the water samples are 0,1792 mg/L, and 0,0647 mg/L. The sum of the fraction of non-resistant and resistant fractions of Cu and Zn in the sediment obtained in this study indicates the total concentration of metals in the sediment, i.e 33,9036 mg / kg and 50,8296 mg/kg. Results of the metal speciation are as follow: fraction of Cu is dominated by resistant fraction that is 64,38 %, whereas non-resistant fractions are: 3,70 % for acid reducible and 31,89% for oxidisable organic. Fraction of Zn is dominated by acid reducible fraction of 39,27 %, followed by organic oxidisable of 25,46 % and EFLE fraction of 1,80%, whereas the resistant fraction is 33,47 %. The percentages of Cu and Zn bioavailabilities respectively are 35,59 % ; and 66,53 %.

KAPASITAS ADSORPSI BEBERAPA JENIS KULIT PISANG TERAKTIVASI NaOH SEBAGAI ADSORBEN LOGAM TIMBAL (Pb) Putu Eka Purnama; I Gusti Ayu Kunti Sri Panca Dewi; Ketut Ratnayani
Jurnal Kimia (Journal of Chemistry) Vol. 9, No. 2 Juli 2015
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Adsorption capacity of various types of banana skin (green, kepok and susu) activated with NaOH as adsorbents for lead (Pb) has been studied. This research consisted of several steps included determination of the surface area of activated and unactivated adsorbents, equilibrium time, adsorption isotherms and adsorption capacity of adsorbents from green, kepok and susu banana skins activated with NaOH . The results showed that adsorbents from green, kepok and susu banana skin activated with NaOH had surface were of 36.2181 m2/g, 35.5531 m2/g and 35.8378 m2/g respectively. On the other hand, the surface area of unactivated adsorbents of green, kepok and susu banana skin were 35.3105 m2/g, 35.3199 m2/g, and 35.7928 m2/g respectively. Equilibrium time for green, kepok and susu banana skin adsorbents activated with NaOH were 30; 30 and 90 minutes. Adsorption isotherms of adsorbents from green, kepok and susu banana skin activated with NaOH were at concentration 100 ppm. Adsorption capacity of activated adsorbents from green banana, kepok banana and susu banana skin were 7.022 mg/g, 5.3078 mg/g and 6.6850 mg/g respectively.

PEMANFAATAN KALENG ALUMINIUM BEKAS DALAM MENANGANI PENCEMARAN AIR WIWIK SUSANAH RITA; I.A. RAKA ASTITI ASIH; WAHYU DWIDJANI S; I W. SUIRTA; I G.A. KUNTI SRI PANCA DEWI
Buletin Udayana Mengabdi Vol 7 No 2 (2008): Volume 7 No.2 – September 2008
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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ABSTRACT Community service by giving a lecture and practical work with a title: “Utilizing of Aluminum Cans used for Water Purification (Pemanfaatan Kaleng Aluminium Bekas dalam Upaya Penjernihan Air)” was be held on Wednesday, 22nd of August 2007. The participants of this activity were about 45 of the Third degree Students of SMAN Abiansemal Badung. The aim of the activity was to give information that aluminum cans can be recycled into alum (tawas), where it can be used for water purification. The result of this activity indicated that the knowledge of the students have broaden. This was showed by Their enthusiastic to give some questions and ideas. They paid attention about the environmental cleanliness, so this activity can help them to solve many environmental problems and motivate them to run a private enterprise.

PEMANFAATAN KOTORAN BABI MELALUI KOMPOSTING DAN PENGARUHNYA TERHADAP PERTUMBUHAN LOMBOK RAWIT (CAPSICUM FRUTESCENS) I W. Budiarsa Suyasa; I G.A Kunti Sri Panca Dewi
Bumi Lestari Journal of Environment Vol 8 No 1 (2008)
Publisher : Environmental Research Center (PPLH) of Udayana University

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The research was conducted about dirt pig compost with variation of composition, Toksicity of it was detected by influence to Growth Of Lombok Rawit ( Capsicum Frutescens). The composition of it is I ( pig dirt 40%, hay 40%, dregs know 18%, dolomit 20%), II ( pig dirt 60%, hay 22%, dregs know 13%, dolomit 2%), and III ( pig dirt 70%, hay 13%, dregs know 15%, dolomit 2%). Growth of microorganism in compost process cover two phase that is ferment phase and maturation Process of composting with composition of III ( pig dirt 70%, hay 13%, dregs know 15%, dolomit 2%) owning best quality. That shown from speed of maturation time posed at stability of temperature and pH reached at period six week, influence to growth of lombok rawit ( Capsicum Frutescens) tried give best level of growth that seen amount of leaf more than composition I and II.

KEMAMPUAN ADSORPSI BATU PASIR YANG DILAPISI BESI OKSIDA (Fe2O3) UNTUK MENURUNKAN KADAR Pb DALAM LARUTAN I.G.A. Kunti Sri Panca Dewi
Bumi Lestari Journal of Environment Vol 9 No 2 (2009)
Publisher : Environmental Research Center (PPLH) of Udayana University

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The capability of sandstone coated with Fe2O3 as adsorbent to reduce lead (Pb) content in solution has been stadied in this research. The adsorption process of Pb in solution by sandstone was carried out by immersing sandstone (uncoated A0) in Pb(NO3)2. Coating sandstone was done using Fe(NO3)3.9 H2O with different concentration as follows : 10 g (A1), 20 g (A2) and 30 g (A3) in 50 ml aquadest. To find out the optimum concentration of Fe(NO3)3 as coating agent. The adsorption process of coated sandstone was done by immersing them in Pb(NO3)2 solution. To determine the time of optimum adsorption, the sandstone (coated and uncoated) were immersed for a period of their equilibrium adsorption time. The absorbance of the filtrat resulted from adsorption process was determined by atomic absorption spectrophotometer at ? 217 nm. The result showed that optimum concentration of Fe(NO3)3 as coating agent was 20 g (A2) with the capability absorbing lead (Pb) 4.653 mg/g. The capability of A3 as adsorbent was 4.644 mg/g and the capability of A1 was 4.529 mg/g while the capability uncoated sandstone as adsorbent was 2.784 mg/g.

Co-Authors A. A. Ayu Septri Juwarni Abd. Rahman As-syakur Anak Agung Istri Agung Mayun Laksmiwati Arik Agustina Atita Arli Tiara Dewa Ayu Puspasari Emmy Sahara I Gede Herry Purnama I Ketut Budiarta I Made Sara Wijana I W BUDIARSA SUYASA I Wayan Suirta I. A. R. Astiti Asih Iryanti Eka Suprihatin Ketut Ratnayani Kristina Indra Yanthy T M. Sudiana Mahendra Muliana Rofida Ni G. A. M. Dwi Adhi Suastuti Ni Ketut Sutiari Ni Komang Ariati Ni Made Suaniti Ni Made Utami Dwipayanti Ni Nyoman Trisnawati Ni Nyoman Trisnawati Ni Putu Ayu Krismayanti Ni Putu Ayu Krismayanti Ni Putu Gita Saraswati Palgunadi Putu Eka Purnama Putu Primantari Vikana Suari Putu Primantari Vikana Suari Putu Suarya WAHYU DWIDJANI S Wahyu Dwijani S. WIWIK SUSANAH RITA

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