Article Per Year (5 Year)
p-Index From 2021 - 2026
1.383
P-Index
This Author published in this journals
All Journal Tropical Animal Science Journal
C. Sumantri
Department of Animal Production and Technology, Faculty of Animal Science, IPB University
Agriculture, Biological Sciences & Forestry Energy

Published : 13 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 13 Documents
Search

 1   2 
Genetic Diversity Analysis and Determination of Specific Alleles of Kuantan Cattle Using Microsatellite Markers R. Misrianti; S. H. Wijaya; C. Sumantri; J. Jakaria
Tropical Animal Science Journal Vol. 45 No. 2 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.2.134

Abstract

Kuantan cattle have an important role in Riau Province, Indonesia. Identification of the genetic diversity of these cattle is important to get the basic information for breeding and conservation strategies. The aim of this research was to identify the genetic diversity of Kuantan cattle using microsatellite markers. A total of thirty-nine DNA samples from three breeds were used in this study. The polymerase chain reaction was conducted using four labeled primers of microsatellite (INRA035, ILSTS06, HEL9 and ETH225). The data were analyzed using GenAlEx 6.41, Cervus 3.0, POPTREE, and STRUCTURE Software. A total of thirty-two alleles were found from microsatellite loci. Two alleles in INRA035 locus 112 and 118 occurred as specific allele candidates for Kuantan cattle. The mean of observed heterozygosity value of the Kuantan-2 population (0.602) was higher than Kuantan-1 (0.471) but lower than Madura (0.688) and Pesisir cattle (0.625). PIC value was higher in HEL9 loci. The dendrogram showed that Kuantan cattle existed at different clusters with Pesisir and Madura cattle. This finding indicated that microsatellite markers successfully distinguished clusters of the cattle and could serve as information for conducting conservation and breeding program.
Differential Expressions of Protamine 1 (PRM1) and Protamine 2 (PRM2) Genes as Markers of Semen Quality in Pasundan Bulls Santoso; A. Gunawan; C. Sumantri; R. I. Arifiantini; Herdis
Tropical Animal Science Journal Vol. 45 No. 4 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.4.423

Abstract

Several gene expressions are related to the success of spermatogenesis. Protamine plays an important role in sperm DNA packaging, spermatogenesis, and sperm quality. This study aimed to isolate RNA from spermatozoa and determine the gene expression profiles of protamine 1 (PRM1) and protamine 2 (PRM2). Six Pasundan bulls aged 5-8 years with a body weight of 380 kg-430 kg were used for this study. The Pasundan bulls were classified into group A (70%-79%) and group B (80%-89%) based on their sperm motility in fresh semen. In this study, correlation analysis was performed between fresh semen characteristics (volume, motility, concentration, abnormality, viability, intact plasma membrane (IPM), DNA integrity) and frozen semen characteristics after thawing (motility, viability, IPM, and DNA integrity). Total RNA was isolated from the frozen sperm pellet, and cDNA was synthesized. Specific PCR primers were used for the transcription of PRM1 and PRM2 from sperm. PRM1 and PRM2 gene expressions were evaluated by qRT-PCR, and ACTB was used as a control. The results showed that progressive sperm motility in the fresh sperm positively correlated with sperm viability and IPM. PRM1 and PRM2 were higher (p<0.05) in group B than in group A. This condition indicates the existence and influence of protamine on the parameters of progressive sperm motility. The expression of PRM1 and PRM2 genes could use as markers of semen quality in Pasundan bulls.
Association and Expression of Cluster of Differentiation 4 (CD4) Gene in IPB-D2 Chicken Related to Immunocompetence Index D. Lestari; S. Murtini; N. Ulupi; A. Gunawan; C. Sumantri
Tropical Animal Science Journal Vol. 46 No. 3 (2023): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2023.46.3.287

Abstract

The CD4 gene plays an important role in the immune process by producing CD4 molecules that aid in producing antibodies. IPB-D2 chickens are selected from IPB-D2 chickens based on IgY concentration and ND antibody titer. This study aimed to analyze the polymorphism of the CD4 gene, unravel the mRNA expression of CD4 gene in IPB-D2 chicken related to the immunocompetence index, and detect the CD4 gene pathway. The total samples used were 100 IPB-D2 G2 chickens aged 21 weeks. Blood samples were collected for ELISA test, HI test, sequencing test, and seca tonsil tissue for relative mRNA expression. Polymorphism and association data were analyzed using MEGAX, FinchTV, SNPstat, and DNAsp. The relative mRNA expression analysis was conducted using qRT-PCR. The pathway analysis of the CD4 gene was performed using the Kyoto Encyclopedia of Genes and Genome (KEGG) pathway analysis. The result showed there were 4 SNPs of the CD4 gene in IPB-D2 chicken, i.e., g.7526 C>T, g.7825 C>T, g.8100 C>A, and g.8157 T>A. All CD4 SNPSs showed no association with IgY concentration and ND antibody titers. Relative mRNA expression shows that IPB-D2 chickens with high ND antibody titers have a higher level of expression when compared to IPB-D2 chickens with low ND antibody titers. Furthermore, pathway analysis showed the CD4 gene involved in the T cell receptor (TcR) signaling process. This study concludes that the CD4 gene is polymorphic and involved in the T cell receptor signaling process. This study demonstrated that polymorphisms of the CD4 gene in IPB-D2 chicken might not contribute to the IgY concentration and ND antibody titer but can serve as a reference in the study of CD4 genes in the other chicken breeds related to the other immunocompetence index.
Co-Authors A. Anggraeni A. Anggraeni A. B. L. Ishak A. Gunawan A. Hafid Asep Gunawan C. Budiman C. Talib D. D. Solihin D. Lestari F. Saputra F. Saputra Herdis Herdis Herdis I. I. Arief I. Khaerunnisa Jakaria Jakaria L. Praharani M. Ardiyana M. F. Al-Habib M. Yusuf Masruroh Muh. Rusdin N. Ulupi R. I. Arifiantini R. I. Arifiantini R. Misrianti S. H. Wijaya S. Murtini S. Murtini S. Murtini S. Murtini Santoso Santoso Santoso T. Sartika