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Journal : REKAYASA

Efektivitas Isolat-Isolat Bacillus sebagai Pengendali Penyakit Bulai dan Pemacu Pertumbuhan Tanaman Jagung pada Kondisi Terkontrol Khoiri, Syaiful; Badami, Kaswan; Pawana, Gita; Utami, Ciwuk Sri
Rekayasa Vol 14, No 2: Agustus 2021
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/rekayasa.v14i2.10270

Abstract

The main disease in maize is downy mildew caused by the fungus Peronosclerospora spp.. This pathogen can cause yield losses of up to 100%. Therefore, efforts to control this disease are continuously carried out, including technical culture, assembly of resistant plants, and use of synthetic fungicides. At the farm level, the use of metalaxyl, synthetic fungicides is the most common practice. On the other hand, it has been reported that some Peronosclerospora groups are starting to become resistant to metalaxyl. These problems lead to the need for alternative controls, for example with biological agents. Biological agents from bacterial groups have been developed to control plant disease, but for downy mildew is still limited. The purpose of this study is to screen and test Bacillus spp. ability to suppress downy mildew and promote the growth of maize. The assay was carried out on seeds by invitro to investigate growth-promoting reactions and also testing under controlled conditions in greenhouses to investigate the suppression ability of downy mildew disease development. The results showed B. polymyxa strain BP18, Bacillus subtilis strain BS41, Bacillus sp. strain BT1, and Bacillus sp. strains can stimulate the growth of corn seedlings and suppress downy mildew. The best isolate in suppressing downy mildew was Bacillus sp. strain BT1 with the smallest AUDPC value (3.94) and the highest protection index (82.71%). It is hoped that these results will find potential isolates and have the potential to be developed into biopesticides and biofertilizers.
Karakter Kuantitatif Melon Generasi S0-S4 Siti Rohmatin; Kaswan Badami; Gita Pawana
Rekayasa Vol 15, No 2: Agustus 2022
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/rekayasa.v15i2.15109

Abstract

One of the obstacles to melon cultivation is the availability of seeds and the high price of melon seeds. This condition causes the need to assemble superior melon varieties with the expected characters. This study aims to form a pure line of melon with certain superior characteristics and can be proposed as a new high-yielding variety in the open-pollinated category. The research was conducted at the Experimental Garden of the Agrotechnology Study Program, Faculty of Agriculture, Trunojoyo University, Madura, from September-December 2022. This study used a randomized block design method. The treatments used were nine pure lines of melon (PK-669, D-162, PK-361, PK-165, PK-114, D-029, D-5089, PK-211, and comparison). Each treatment was repeated three times, and each experimental unit consisted of 12 plants. Research data were analyzed using analysis of variance (ANOVA) and HSD test α = 0.05. The selection advancement was analyzed by comparing character data for each generation. The research showed that the results of the characterization of ten melon lines S0-S4 generation on the characters of fruit length, fruit diameter, flesh thickness, and melon fruit weight showed a decreasing trend in each generation. The S4 generation (9.17 °brix) in the character of the average sugar content there was an increase that was not significantly different from the S3 generation (9.03 °brix), while the number of seeds in each generation had an average value that was not significantly different but occurred an increase in the number of seeds in the S3 (372) and S4 (381) generations. An increase in one generation of the population in the linear regression equation is estimated to reduce the character of fruit length by 0.50 cm, fruit diameter by 0.465 cm, flesh thickness by 0.140 cm, fruit weight by 0.199 Kg, sugar content by 0.752 °brix, and a number of seeds by 5.4.