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All Journal HAYATI Journal of Biosciences Hemera Zoa Media Veteriner Forum Pasca Sarjana Jurnal Veteriner Ecotrophic, Journal of Environmental Science Microbiology Indonesia BERITA BIOLOGI Jurnal Matematika Sains dan Teknologi Indonesian Journal of Natural Pigments
BIBIANA W LAY
Department Of Biology, Faculty Of Biotechnology, Atma Jaya Catholic University, Jalan Jenderal Sudirman No. 51, Jakarta 12930, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Mathematics Medicine & Pharmacology Veterinary Other

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Peranan Zona Pelusida Sebagai Barier Terhadap Cemaran Escherichia coli K99 (THE ROLE OF ZONA PELLUCIADA AS A BARRIER OF E COLI K99 CONTAMINATION) I Wayan Batan; Bibiana Widiati Lay; Ita Djuwita; Supar .; Arief Boediono
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the study was to investigate the role of zona pelucida (ZP) as a barrier of embryo againstE.coli K99 contamination.   The complete randomized design was used in this study.  The embryos weregiven treatment as a follow : 1) embryos without ZP were contaminated with E.coli K99;  2) embryo withintact ZP were contaminated with E.coli K99;  and  3) embryos  with intact ZP were not contaminated withE.coli K99, as a control.  In each treatment there was 15 replication and in each replication there was oneembryo.  The embryos were incubated in incubator at 37°C and 5% CO2 atmosphere.  The embryos wereobserved every six hours in 24 hours using inverted microscope.  The result showed that embryos withintact ZP could develop in culture contaminated with  E.coli K99, while embryos without ZP becomedegenerated.  The viability of intact embryos was 75% and the embryos without ZP were 65%.  Embryosculture in contaminated medium could develop from eight cells embryo into morulla stage of embryo,compact morulla, and blastocyst. E.coli K99 contamination could inhibit embryo development.  In conclusion,ZP could protect embryo against E.coli K99 contamination.
PENGOLAHAN LIMBAH TEKSTIL SISTEM KOMBINASI ANAEROBIK-AEROBIK MENGGUNAKAN BIOFILM BAKTERI KONSORSIUM DARI LUMPUR LIMBAH TEKSTIL I Dewa K. Sastrawidana; Bibiana W. Lay; Anas Miftah Fauzi; Dwi Andreas Santosa
ECOTROPHIC : Jurnal Ilmu Lingkungan (Journal of Environmental Science) Vol 3 No 2
Publisher : Master Program of Environmental Science, Postgraduate Program of Udayana University

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The objective of this research are to assess the eficiency of biodegradation of textile dyes by using bacteriaconsortium biofim on combined anaerobic-aerobic system and also to asses the possibility for applicating this technologyin textile dyeing industry. Textile wastewater that used in this research are azo dyes total concentration 200 mg/L which isconsist of remazol yellow, remazol red, remazol black and remazol blue For anaerobik condition step, by using bacteriaconsortium selected from sludge of textile wastewater plant of Mama & Leon consist of Aeromonas sp ML6., Aeromonassp. ML14, Aeromonas sp. ML24, Pseudomonas sp. ML8 and Flavobacterium Sp ML20 whereas aerobic condition step,using bacteria consortium selected from Badung river sludge consist of Vibrio sp. and Plesiomonas sp. Both bacteriaconsortium is immobilized separatively on volcanous stone for 3 days in each reactor before used to treatment of synteticdyeswater (SDW). Further more, immobilized bacteria consortiom on volcanous stone is determined by four plate countmethod.The result ot this research showed the eficiency process in decreasing color, TDS, TSS, COD and BOD was96,94%,75,73%, 68,03%, 97,68% dan 94,60% respectively. So, wastewater treatment by using combined anaerobicaerobicsystem is potential to applied on wastewater treatment in textile industry.
Isolation and Identification of Bacteria from Raw Materials Contaminated by Rope-producing Bacteria ANASTASIA ASRI WIDYASARI; STELLA MAGDALENA; BIBIANA WIDIYATI LAY
Microbiology Indonesia Vol. 9 No. 3 (2015): September 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (328.587 KB) | DOI: 10.5454/mi.9.3.3

Abstract

Ropey bread is a bacterial spoilage condition of bread. The spoilage involved in ropey bread is primarily due to Bacillus subtilis. Several studies have shown that rope spoilage can be controlled using antimicrobial agents such as acetic acid, lactic acid, and quaternary ammonium cations (QACs/quats). This research consisted of five main steps: isolation, identification, confirmation, and molecular characterization of bacteria from raw materials contaminated by rope-producing bacteria, and antimicrobial test against rope-producing bacteria. The confirmation test was done in order to determine the rope-producing ability of isolates suspected as Bacillus sp. There were two different treatments in this test. In the first treatment, the inoculums were mixed with bread dough. In the second treatment, each slice of loaf was placed into a petri dish, uniformly soaked with inoculums. The first treatment did not show rope spoilage for all of the loaves, however, 6 of 11 loaves in the second treatment developed rope. The largest inhibition halos for antimicrobial test were produced by quats. This means quats is the strongest antimicrobial agent against rope-producing bacteria. The molecular characterization showed that all of the suspected isolates had 98–99% similarity to Bacillus subtilis.
Co-Authors A.A. Ketut Agung Cahyawan W Aisjiah Girinda Anas Miftah Fauzi ANASTASIA ASRI WIDYASARI Arief Boediono DWI ANDREAS SANTOSA Ekowati Handharyani Etty Riani Felianti, Felianti Hermonoadi Huminto Hernomoadi . Hernomoadi Hoeminto I Dewa Ketut Sastrawidana I Nyoman Suartha I Wayan Batan I wayan Teguh Wibawan Ita Djuwita KHUSNUL YAQIN latifah K Darusman Latifah K. Darusman M.B. Malole . Nurhasanah . Nurhasanah Nurhasanah Retno Damayanti Soejoedono Stella Magdalena Sugyo Hastowo Supar . Surjono Hadi Sutjahjo Warjoto, Renna Eliana Wiwin Winarsih