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All Journal Jurnal Ilmu Kedokteran (Journal of Medical Science) Medical Journal of Indonesia Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia Indonesian Journal of Legal and Forensic Sciences
Sugiharto, Ade Firmansyah
Departemen Forensik Dan Medikolegal, Rumah Sakit Cipto Mangunkusumo - Fakultas Kedokteran Universitas Indonesia
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health

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Penentuan Umur Bercak Darah Manusia pada Kain Katun dengan Menggunakan High Performance Liquid Chromatography Mohammad Tegar Indrayana; Wibisana Widiatmaka; Ade Firmansyah Sugiharto; Ridla Bakri
Jurnal Ilmu Kedokteran Vol 8, No 1 (2014): Jurnal Ilmu Kedokteran
Publisher : Fakultas Kedokteran Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.923 KB) | DOI: 10.26891/JIK.v8i1.2014.8-17

Abstract

This research is to study the method in determining the age of human bloodstain due the rarity of human bloodstainresearches in Indonesia, by analyzing the X and Y peaks that appeared in High Performance Liquid Chromatography(HPLC). This research is experimental with time series for 11 days observation by exposing the bloodstain onto 33pieces of cotton fabric, each measuring 1 cm x 1 cm that originated from 3 respondents with environmental conditionsof the FKUI/RSUPN Dr. Cipto Mangunkusumo Forensic and Medico-legal Department which represents the area ofCentral Jakarta. The results of this research is the X and Y peaks do not point to a linearity so that the criteria for linearregression equation is not met. The conclusion of this research is age of human bloodstain on cotton fabric byanalyzing X peak with HPLC cannot yet be used pragmatically in the field in accord to the conditions at the crimescene.
DNA quality and quantity in adipose tissue: a comparison of the effects of bomb explosion Leonardo; Sugiharto, Ade Firmansyah; Indriatmi, Wresti; Atmadja, Djaja Surya; Yudianto, Ahmad; Herkutanto; Widodo, Wahyu
Medical Journal of Indonesia Vol. 32 No. 4 (2023): December
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.oa.247206

Abstract

BACKGROUND Adipose tissue is often overlooked in DNA testing due to misconceptions about its DNA content. However, its shock-absorbing qualities may be useful for high-pressure scenarios like bomb blasts. This study aimed to evaluate DNA quality and quantity in adipose tissue affected by blasts compared to that in unaffected tissue. METHODS 10 adipose tissue samples were taken from regions near and far from the blast, representing the blast-exposed and non-blast-exposed groups. The adipose tissue was stored at a low temperature for 5 days, after which an organic extraction method was applied. The purity of the DNA extract was assessed using a NanoDrop spectrophotometer, and its integrity was evaluated using 0.8% concentration gel electrophoresis at 60 V for 90 min. DNA typing was conducted using the GlobalFiler™ kit, and DNA quantity was determined with the Quantifiler™ Trio DNA Quantification kit. RESULTS Of 20 DNA extracts from adipose tissue, all samples demonstrated purity, integrity, and complete typing results. Adequate integrity was found in 90% of samples in both groups. A 50% incidence of allele shifting was observed at the D7S820 locus within the blast-exposed group. CONCLUSIONS DNA from blast-exposed adipose tissue exhibited no significant quality or quantity differences from non-blast-exposed tissue. This suggested adipose tissue’s potential as an alternative DNA source in a bomb explosion.
Perbandingan Teknik Deteksi Restorasi Resin Komposit dalam Identifikasi Gigi pada Mayat (Review) Fahriza Rizki Oktaviana; Elza Ibrahim Auerkari; Ferry Pergamus Gultom; Ade Firmansyah Sugiharto
Indonesian Journal of Legal and Forensic Sciences Vol. 14 No. 1 (2024): Indonesian Journal of Legal and Forensic Sciences
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJLFS.2024.v14.i01.p04

Abstract

Peningkatan kualitas resin komposit dari segi estetika dapat mempersulit ahli odontologi forensik untuk mengidentifikasi jenazah. Komposisi resin komposit yang ada membuat restorasi gigi terlihat alami karena sifat translusensi, fluoresensi, opalesensi, dan adaptasi margin yang baik. Selain itu, pada bencana massal, identifikasi korban yang dilakukan di lokasi yang tidak memiliki akses air dan listrik, akan menghambat proses pemeriksaan visual, terutama karena adanya cairan pembusukan dan darah pada rongga mulut mayat dan adanya kondisi rigor mortis (kesulitan membuka mulut jenazah). Review ini bertujuan untuk menganalisis metode yang dapat digunakan untuk mendeteksi restorasi resin komposit pada jenazah manusia dan implikasinya dalam odontologi forensik. Ada berbagai teknik untuk mengidentifikasi restorasi komposit dalam pemeriksaan forensik. Perbandingan radiografi gigi ante-mortem dan post-mortem adalah teknik yang paling umum digunakan untuk membedakan tambalan resin komposit dari struktur gigi asli (email dan dentin), tetapi tidak dianjurkan untuk mendeteksi restorasi kecil. Namun, fluorescence-aided identification technique (FIT) menunjukkan akurasi yang lebih tinggi dalam hal ini karena perbedaan intensitas fluoresensi antara restorasi dan struktur gigi. Metode optik lainnya seperti FOTI (fiber optic trans-illumination) dan DiFOTI (digital fiber optic trans-ilumination) dianggap sebagai alternatif tetapi FIT (fluorescence-aided identification technique) tetap lebih unggul dibandingkan teknik ini. Teknik pewarnaan juga menjadi pilihan untuk identifikasi tambalan komposit jika fluoresensi tidak dapat dibedakan. Investigasi lebih lanjut diperlukan untuk menemukan teknik terbaik atau kombinasi teknik untuk menghindari kesalahan dokumentasi dalam pemeriksaan gigi forensik. Kata kunci: restorasi resin komposit; identifikasi gigi; teknik fluorescence-aided identification; radiografi gigi; teknik pewarnaan; odontologi forensik
Aspek Biomekanika Forensik Pada Trauma Peluru Plastik Airsoft Gun Ade Firmansyah Sugiharto; Ira Zefanya Pattihahuan
Majalah Kedokteran Indonesia Vol 75 No 1 (2025): Journal of The Indonesian Medical Association - Majalah Kedokteran Indonesia, Vo
Publisher : PENGURUS BESAR IKATAN DOKTER INDONESIA (PB IDI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47830/jinma-vol.75.1-2025-1854

Abstract

The biomechanical aspects of trauma caused by airsoft gun plastic pellets depend on the energy generated by the pellet and the tissue resistance. The airsoft gun plastic pellet creates lower energy compare to firearm, thus creating non-lethal effect to human body, but sufficient for creating tissue damage. This energy is determined by the velocity, weight, and diameter of the plastic pellet.. Indonesia allows for a maximum of 2 Joule energy, 8 mm in diameter, and 0,4 gram in maximum weight for airsoft gun plastic pellet. When it hits body surface, this characteristics will create energy of 3,98 J/cm2, and 7 J/cm2 with 6 mm plastic pellet diameter. The most common case of injury due to airsoft gun plastic pellet is eye ball injury. With a resistance of 4.3-4.8 J/cm², trauma from airsoft gun plastic pellets can result in corneal abrasion, hyphema, contusion, and even rupture.. Skin has much higher resistance, which is 23,99 J/cm2. Some injury may still be found, including erythema and 5 mm skin penetration.
Co-Authors Ahmad Yudianto Djaja Surya Atmadja Elza Ibrahim Auerkari Fahriza Rizki Oktaviana Ferry P. Gultom, Ferry P. Ganda, Michael Herkutanto Idham R. Dewantara Ira Zefanya Pattihahuan Leonardo Mohammad Tegar Indrayana Muhammad Ilham Dhiya Rakasiwi Muhammad Taufik Ridla Bakri Wahyu Widodo Wibisana Widiatmaka Wresti Indriatmi B. Makes Yuli M. Shufiyani