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All Journal Microbiology Indonesia LEMBARAN PUBLIKASI MINYAK DAN GAS BUMI Scientific Contributions Oil and Gas
CUT NANDA SARI
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Medicine & Pharmacology

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Construction and Expression of Single Recombinant Peptide Surfactant for EOR Application CUT NANDA SARI; USMAN USMAN; RIESA KW ROHMAT; LENI HERLINA; KEN SAWITRI SULIANDRI; ONIE KRISTIAWAN; DWIYANTARI DWIYANTARI; TATI KRISTIANTI; SONY SUHANDONO
Microbiology Indonesia Vol. 11 No. 1 (2017): March 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1420.163 KB) | DOI: 10.5454/mi.11.1.5

Abstract

Surfactant is generally synthetic chemical, which is effective and reliable. However, the chemicals usually did not degraded easily in the environment and could cause damage to the environment. The other possible alternative to produce surfactant is using genetic engineering in order to produce peptide based surfactant. In this research, peptide surfactant was produced using a gene construct which was created using overlapped polymerase chain reaction method (OE-PCR). PAGE analysis shows that single surfactant peptide construction can be expressed by induction of IPTG 1 mM and after at least twice sonication. This research proves that both two constructions have been successfully expressed by producing peptide in expected size (approximately 15 kDa).
PENGAMATAN MEOR MENGGUNAKAN FORMULA NUTRISI RENDAH GLUKOSA DENGAN METODA IMBIBISI Sugihardjo; Zulkifliani; Onie Kristiawan; Cut Nanda Sari; Syafrizal
Lembaran Publikasi Minyak dan Gas Bumi Vol. 53 No. 1 (2019): LPMGB
Publisher : BBPMGB LEMIGAS

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Abstract

Kegiatan skala laboratorium telah dilakukan untuk merancang formula nutrisi MEOR (Microbial Enhanced Oil Recovery) dengan bahan dasar rendah-glukosa. Bahan yang dipakai meliputi: limbah cair tahu, air kelapa, limbah cair ikan, limbah pengolahan pati, ekstrak teh, pupuk cair, ekstrak daging sapi, dan pepton. Bahan-bahan tersebut diracik untuk menjadi beberapa formula yang dapat merangsang pertumbuhan mikroba yang terkandung pada percontoh fluida dari sumuran SMR-01, SMR-02, dan SMR-03 sehingga menghasilkan bioproduk yang diperlukan untuk MEOR Pada seleksi kajian awal ada 48 formula nutrisi, kemudian diseleksi dan diperoleh yang potensial dalam pertumbuhan mikroba sebanyak 8 formula, yaitu 2 macam formula untuk SMR-01, 3 untuk SMR-02, dan 3 juga untuk SMR-03. 8 formula tersebut diinkubasi selama 7 hari dan diamati bioproduknya yang meliputi: pertumbuhan mikroba, pH, IFT, densitas, dan viskositas minyak. Pada tiap formula juga ditambahkan konsorsium mikroba exogenous untuk memperkaya jenis mikroba. Uji imbibisi dilakukan terhadap 8 formula tersebut serta ditambah 8 formula lagi dengan menambahkan konsorsium mikroba exogenous selama 71 hari. Hasil yang terbaik ternyata ada pada formula Ef1 dengan penambahan ekstrak teh dan pepton dan Ez1 dengan tambahan ekstrak teh dan ekstrak beef pada fluida SMR-02. Dengan nilai masing masing nilai RF (recovery factor) 56,91 untuk Ef1 dan 55,86% untuk Ez1. Oleh karena itu kedua formula tersebut dapat dijadikan acuan untuk implementasi lapangan. Secara ekonomis mungkin Ez1 akan lebih murah karena prosentase kandungan extract beef hanya 10%.
KONSTRUKSI DAN EKSPRESI REKOMBINAN TUNGGAL PEPTIDA SURFAKTAN (SINGLE SUPEL CONSTRUCTION) UNTUK APLIKASI EOR Cut Nanda Sari; Usman P; Riesa K. W. Rohmat; Leni Herlina; Ken Sawitri Suliandari; Onie Kristiawan; Dwiyantari; Tati Kristianti; Sony Suhandono
Lembaran Publikasi Minyak dan Gas Bumi Vol. 50 No. 3 (2016): LPMGB
Publisher : BBPMGB LEMIGAS

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Abstract

Surfaktan yang digunakan pada aplikasi peningkatan perolehan minyak tahap lanjut pada umumnya merupakan hasil sintesis kimia. Hasil sintesis ini bersifat cepat dan efektif namun secara kuantitas sangat kecil, sehingga bila dibutuhkan dalam jumlah banyak akan membutuhkan banyak biaya untuk memproduksinya. Alternatif lain yang bisa digunakan untuk menghasilkan surfaktan adalah dengan rekayasa genetika melalui produksi rekombinan dalam mikroorganisme seperti bakteri untuk menghasilkan surfaktan berbasis peptida. Teknologi ini relatif murah dan simpel untuk dilakukan yaitu dengan manipulasi ekspresi sel inang agar menghasilkan peptida surfaktan yang dikonstruk kedalam vektor ekspresi berbasis bakteri. Pada penelitian ini dilakukan konstruksi peptida surfaktan dengan menggunakan metode overlaped reaksi berantai polimerase untuk menghasilkan surfaktan peptida sebagai peptida tunggal. Hasil analisis SDS PAGE (Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis) menunjukkan konstruksi peptida surfaktan tunggal dapat diekspresikan dengan cara diinduksi IPTG 1 mM dan dilakukan pemecahan sel untuk mendapatkan protein yang diproduksi diperiplasma. Penelitian ini membuktikan bahwa kedua konstruk berhasil diekspresikan dengan menghasilkan peptida pada ukuran yang sesuai.
BIOREMEDIATION IN PETROLEUM CONTAMINATED SOIL TREATMENT USING PLANT-MICROORGANISMS COMBINATION (Case Study: Reduction Level of TPH and BTEX in Bioremediation Process) Cut Nanda Sari; Tyas Putri Sativa; Setyo Sarwanto Moersidik
Scientific Contributions Oil and Gas Vol 39 No 1 (2016)
Publisher : Testing Center for Oil and Gas LEMIGAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29017/SCOG.39.1.532

Abstract

Oil spills, in both aquatic and terrestrial environments, are very detrimental to people and the environment due to hydrocarbon compounds that are contained in oil which are not only be harmful for the balance of the ecosystem and the environment but also carcinogenic to humans and animals. Therefore remediation needs to be done. One of the methods is by using a combination of microorganisms and plants. The aim of this research is to analyze the in􀃀 uences between several different treatments that are applied for TPH and BTEX removal in the process of remediation. In this research, bioremediation was conducted by using four different treatments which are: by adding compost (C), plants and compost (P), microorganisms and compost (B), and compost, plants and microorganisms (BP), to soil with oil content of 5% and 10%. The following test results of TPH in soil contaminated with 5% oil content are: 2.10% (C); 1.31% (B); 1.66% (P); and 0.68% (BP). The TPH test results in soil contaminated with oil content of 10% are: 3.30% (C); 2.54 (B); 3.91% (P); and 3.31% (BP). The highest percentage of TPH degradation in contaminated soil of 5% oil content was found in BP treatment (87.1%), while in the contaminated soil of 10% oil content the largest TPH removal percentage is by the treatment of adding bacteria (B) which is 76.19%. BTEX removal percentage in 5% oil contaminated soil in BP treatment is 68.35% while in 10% oil contaminated soil with B treatment the removal percentage is 84.91%. Based on statistical tests, both on contaminated soil with 5% and 10% oil content, TPH degradation signi􀂿 cantly affects the pH value as p < 0.05 but TPH degradation does not affect temperature values as p > 0.05.
Dilute Acid Pretreatment And Enzymatic Hydrolysis Of Lignocellulosic Biomass For Butanol Production As Biofuel Devitra Saka Rani; Cut Nanda Sari
Scientific Contributions Oil and Gas Vol 35 No 1 (2012)
Publisher : Testing Center for Oil and Gas LEMIGAS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29017/SCOG.35.1.776

Abstract

Biobutanol is one of the promising biofuel for substituting gasoline. Biobutanol produced from biomass fermentation using solventogenic clostridia which are able to convert a wide range of carbon sources to fuels such as butanol. Therefore, lignocellosic biomass has great potential as fermentation substrate for biobutanol production. Lignocellosic biomass should be hydrolized before fermentation by a pretreatment process and enzymatic hydrolysis. The various lignocellulosic biomass pretreatment will infl uence in butanol production depending on fermentable sugars content. The objective of this research is to get potential lignocellulosic biomass using dilute acid pretreatment and enzymatic hydrolysis process for biobutanol production. Eight types of biomass from sugarcane bagasse, rice straw, rice husk, empty fruit bunch (EFB) of palm oil, corn cob, pulp waste, traditional market organic waste, and microalgae were used in this experiment. After hydrolysis, the high result of total fermentable sugars in corn cobs, bagasse, rice straw, and rice husk, shows good opportunity of these biomass to be used as fermentation feedstocks for biobutanol production. In addition, pulp waste, organic waste, and microalgae are prospective as raw material but require more appropriate treatment either for to break down the cellulose/hemicellulose or to enhance reducing sugar content. Fine milling and delignifi cation have no signifi cant effect on cellulosic biomass conversion into fermentable sugars. Therefore, the production cost can be reduced. In order to enhance the sugar content and reduce the formation of inhibitor product, it is necessary to examine dilute acid pretreatment variations and appropriate operating conditions of enzymatic hydrolysis process
Co-Authors Devitra Saka Rani Dwiyantari DWIYANTARI DWIYANTARI Ken Sawitri Suliandari Ken Sawitri Suliandari KEN SAWITRI SULIANDRI Leni Herlina LENI HERLINA Lina Lubnah Nafian Awaludin Onie Kristiawan ONIE KRISTIAWAN Refiana Lestary Riesa K. W. Rohmat Riesa Khairunnisa W.R. RIESA KW ROHMAT RUKMAN HERTADI Setyo Sarwanto Moersidik Sony Suhandono SONY SUHANDONO Sugihardjo syafrizal Tati Kristianti TATI KRISTIANTI Tegar Nurwahyu Wijaya Tyas Putri Sativa Usman Usman P Usman Usman Zulkifliani