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All Journal Jurnal Akuakultur Indonesia BERITA BIOLOGI Jurnal Mina Sains
Angela Mariana Lusiastuti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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APLIKASI VAKSIN Streptococcus agalactiae UNTUK PENCEGAHAN PENYAKIT STREPTOCOCCOSIS PADA BUDIDAYA IKAN NILA (Oreochromis niloticus) Taukhid Taukhid; Angela Mariana Lusiastuti; Tuti Sumiati
BERITA BIOLOGI Vol 13, No 3 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i3.662

Abstract

The research with the aim to know the effectivity (yield gap) of the application of Streptococcus agalactiae vaccine (pure whole cell) in prevention of streptococcosis on tilapia (Oreochromis niloticus) culture has been carried out. The isolate of S.agalactiae – N14G was used as a master seed on vaccine production. Priming vaccination was administered by immersion method, and booster vaccination was taken th place two months latter by oral method. Challenge test at the lethal dose (LD50) against active bacteria was done at 14 days post booster vaccination, and observation was taken place for 14 days post artificial infection. The results of the research showed that the highest survival rate and relative percent survival (RPS) was found in group treated with Streptovac vaccine (S. agalactiae and A. hydrophila combination) (65.58% and 35.36%) followed by S. agalactiae vaccine (52.08% and 10.01%). The lowest survival rate was found in control group (46.75%). The result of confirmation effectivity of the vaccines by challenge test in the laboratory showed that the highest survival rate and relative percent survival (RPS) was found in S. agalactiae vaccine (50.00% dan 37.50%) followed by Streptovac vaccine (40.00% and 25.00%), and the lowest survival rate was found in control group (20.00%). Vaccination is better than the non vaccinated.
Efektivitas Pemberian Ekstrak Kipahit (Tithonia diversifolia) Sebagai Imunostimulan Untuk Pencegahan Motile Aeromonas Septicemia (MAS) Pada Ikan Patin (Pangasionodon hypophthalmus) Edwina Edwina; Mulyana Mulyana; Angela Mariana Lusiastuti
JURNAL MINA SAINS Vol. 3 No. 1 (2017)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (856.633 KB) | DOI: 10.30997/jms.v3i1.862

Abstract

Motile Aeromonas Septicemia (MAS) merupakan penyakit yang sering ditemui pada kegiatan budidaya, dan salah satu komoditas yang sering terserang oleh penyakit ini adalah ikan patin.  Penelitian ini bertujuan mendapatkan dosis terbaik dari simplisia tanaman kipahit untuk mencegah (MAS) pada ikan patin. Percobaan dilaksanakan tanggal 10 Januari 2014 sampai dengan 22 Maret 2014 bertempat di Balai Penelitian dan Pengembangan Budidaya Air Tawar, Sempur, Bogor. Ikan patin dipelihara selama 28 hari sebelum uji tantang dan 10 hari setelah uji tantang dengan perlakuan pakan menggunakan campuran simplisia kipahit dengan dosis 2,44%; 4,76%; 9,09%; dan 16,67%. Perlakuan kontrol adalah pakan komersil tanpa penambahan simplisia kipahit. Hasil penelitian menunjukkan bahwa pemberian pakan seluruh dosis perlakuan, baik sebelum maupun sesudah uji tantang tidak mempengaruhi nilai tingkat kelangsungan hidup, rasio konversi pakan, dan laju pertumbuhan spesifik ikan patin. Pemberian perlakuan setelah dilakukan uji tantang diduga memberikan pengaruh terhadap respon imun terhadap ikan patin, tetapi tidak memiliki aktivitas bakterisidal. Dosis simplisia kipahit terbaik untuk pencegahan MAS pada ikan patin adalah 16,67% karena pada dosis tersebut aktivitas imunostimulan bekerja lebih baik dibandingkan dosis yang lainnya, serta aman terhadap organ hati berdasarkan uji fungsi hati.Kata kunci : Ikan patin, kipahit, Motile Aeromonas Septicemia, pakan.
Pathogenicity test bacteria from Oreochromis niloticus and Clarias gariepinus aquaculture ponds Hardi, Esti Handayani; Halim, Aldi M.; Nugroho, Rudy Agung; Mawardi, Mira; Isnansetyo, Alim; Mariana Lusiastuti, Angela; Rahayu, Widianingsih; Niklani, Andi; Saptiani, Gina
Jurnal Akuakultur Indonesia Vol. 22 No. 1 (2023): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.22.1.97-105

Abstract

Present research aimed to examine the pathogenicity opportunities of some bacteria which were isolated from freshwater aquaculture ponds to some fish Oreochromis niloticus, Clarias gariepinus, and Pangasius pangasius. The bacteria were isolated from the water and feces of O. niloticus and C. gariepinus aquaculture in Samarinda, which were identified as Escherichia coli, Enterobacter cloacae, and Enterobacter amnigenus. An 0.1 mL-1 of each bacteria was intramuscular injection and evaluated the clinical signs, eksternal pathology, and fish mortality. The freshwater fish using in this research were Oreochromis niloticus, Clarias gariepinus, and Pangasius pangasius with size 10-15 g from Loa Kulu, Kutai Kartanegara Regency, East Kalimantan, Indonesia. The result showed that each bacteria caused mortality in fish: E. coli bacteria caused fish mortality 23.33-66.67%; E. cloacae 10.00 to 90.00%; and E. amnigenus by 3.33-56.67%. The average death time of E. coli and E. amnigenus in all three fish were under 48 hours, while E. cloacae caused mortality less than 48 hours in C. gariepinus and P. pangasius. In conclusion, E. coli, E. cloacae, and E. amnigenus are putative pathogenic bacterium in O. niloticus, C. gariepinus, and P. pangasius.
Co-Authors ALIM ISNANSETYO Edwina Edwina Enang Harris Esti Handayani Hardi Gina Saptiani Halim, Aldi M. Hilma Putri Fidyandini Khairun Nissa Laksono Trisnantoro M. Zairin Junior Mira Mawardi Mulyana Mulyana Niklani, Andi Rahayu, Widianingsih Rudy Agung Nugroho Sri Nuryati Sukenda . Sukenda . Taukhid Taukhid Tuti Sumiati