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All Journal Jurnal Akuakultur Indonesia Jurnal Veteriner BERITA BIOLOGI Jurnal Mina Sains
Angela Mariana Lusiastuti
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

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Benih Keturunan Induk Ikan Nila yang Divaksinasi pada Tingkat Kematangan Gonad-2 Lebih Tahan Terhadap Infeksi Streptococcus agalactiae (RESISTANCE OF TILAPIA (OREOCHRIMIS NILOTICUS) FRY VACCINATED AT DIFFERENT GONADAL DEVELOPMENTAL STAGES TOWARD STREPTOCO Khairun Nissa; Sukenda Sukenda; Muhammad Zairin Junior; Angela Mariana Lusiastuti; Sri Nuryati
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to evaluate the effectiveness of vaccination based on gonad maturationstages on tilapia brood stocks in which the released antibodies was able to be transferred to the seed.Vaccine composed with whole cells and extracellular product (ECP) was injected at stage 2 and stage 3 ofthe gonad development stages at concentration of 109 CFU mL1 as much as 4 mL to 1 kg of brood fish.Control fish was unvaccinated treatment. Challenge study at seed was conducted by immersing S. agalactiaefor 30 minutes at 7, 14, 21, and 28 days post hatching (DPH) in 107 CFU/mL. Antibody levels on broodstocks, eggs, and body fluids of seed, and relative percentage survival (RPS) of seed post challenge studywere evaluated. The results showed that stage 2 of gonad developmental stages was found on 7 days postinitial spawning and stage 3 found on 14 days post initial spawning of brood fish. Vaccinated done in stage 2 of gonad developmental stages gave immunoglobulin serum in brood (0,166±0,001), egg (0,165±0,002),and seed aged 7, 14, 21, and 28 days post hatching (0,164±0,002, 0,162±0,005, 0,155±0,006, and 0,14±0,008respectively) were significantly higher (P<0,05) compared to other treatment. Challenged test that doneby immersing with S. agalactiae suspension on larval aged 7, 14, 21, and 28 days had highest RPS(95,24%, 83,33%, 72,22%, and 56,02% respectively) formed on seed from brood stock vaccination in gonaddevelopment stage 2. Vaccination in tilapia brood stocks at stage 2 of gonad developmental stages gavehighest protection by maternal immunity to the seed against S. agalactiae.
Pemberian Probiotik Multispesies dalam Media Budi Daya Ikan Lele Dumbo untuk Mencegah Penyakit Motile Aeromonads Septicemia (ADDITION OF MULTISPECIES PROBIOTICS IN THE CULTURE MEDIUM OF AFRICAN CATFISH TO PREVENT THE MOTILE AEROMONADS SEPTICEMIA DISEASE) Hilma Putri Fidyandini; Munti Yuhana; Angela Mariana Lusiastuti
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

One of the disease that often led to mortality in catfish resulting in harvest failure is Motile AeromonadsSepticemia (MAS) caused by infection of Aeromonas hydrophila. To avoid this situation, the preventionand control of disease is very crucial. An environmentally friendly approach acceptable in aquaculture isthe use of probiotics to control the pathogenic infection. African catfishes used in the study weighted of1,97±0,7g/fish, and were maintained in the aquarium size of 56x39x34 cm3filled with 30 liters of waterwith stock density of 25 fishes . Probiotic was given daily for 28 days. This research was consisted of 6treatments; (A) AH26 NAR103 CFU/mL, probiotics ND2 CefR 105 CFU/mL dan P23 CipR 105 CFU/mL ; (B)AH26 NAR103 CFU/mL, probiotics ND2 CefR 103 CFU/mL dan L1k TetR 103 CFU/mL; (C) AH26 NAR103CFU/mL, probiotics P23 CipR 104 CFU/mL dan L1k TetR 104 CFU/mL; (D) AH26 NAR103 CFU/mL, probioticsND2 CefR 105 CFU/mL, P23 CipR 105 CFU/mL dan L1k TetR 105 CFU/mL; (K+) AH26 NAR103 CFU/mL, without probiotics; (K-) Without the addition of probiotics and without AH26 NAR in the media. Eachtreatment has 3 replications. The results showed that the combination of probiotics ND2CefR103CFU/mLand L1k TetR103CFU/mLcan supressedA. hydrophila cell density up to 40% lower, induced immuneresponses (hematocrit level and respiratory burst activity) and increased the survival rate of catfishuntilthe end of the research.
Kandidat Vaksin Potensial Streptococcus agalactiae untuk Pencegahan Penyakit Streptococcosis pada Ikan Nila (Oreochromis niloticus) (POTENTIAL VACCINE CANDIDATE OF STREPTOCOCCUS AGALACTIAE FOR PREVENT STREPCOCOCOSIS ON NILA TILAPIA (OREOCHROMIS NILOTICUS) Esti Handayani Hardi; Sukenda .; Enang Harris; Angela Mariana Lusiastuti
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The effectiveness of Streptococcus agalactiae vaccine in tilapia (Oreochromis niloticus) was evaluatedfor prevention of streptococcal disease. The vaccine was prepared using formalin-killed whole cell andconcentrated extracellular products/ECP (62.3 and 55.8 kDa) of â-haemolityc isolate and 62.3; 55,8 and51.8 kDa protein of  non-haemolityc ECP of S. agalactiae.  Vaccination and challenged (103 colony-formingunits (CFU)/fish of â-haemolityc and 105 CFU/fish of non-haemolityc S. agalactiae) trial was conducted byintraperitonial (IP) injection into fish with average body weight of 15 g.  Fish were vaccinated with wholecell, ECP and mixed (whole cell and ECP) vaccine.  Tilapia vaccinated with whole cell of â-haemolitycisolate had a relative percent survival (RPS) rates higher than those of ECP â-haemolityc vaccine. However,fish  vaccinated with mixed (whole cell and ECP) of â-haemolityc has a better protection rates as comparedto those of two type of S. agalactie infection. Whereas those vaccinated with mixed (whole cell non-haemolitycand ECP of â-haemolityc) vaccine has protection rate of 79% from â-haemolityc and 42% from non-haemolitycinfection.  Tilapia vaccinated with whole cell of non-haemolityc was only able to protect fish from non-haemolityc infection and was unable to protect fish from other types.  Tilapia vaccinated with ECP non-haemolityc had a worse RPS than others vaccines in which mix whole cell and ECP vaccine of non-haemolitychad a protection 50-56% from  S. agalactiae infection. Whereas vaccinated with mixed (whole cell â-haemolityc and ECP of non-haemolityc) vaccine showed a better to protect from â-haemolityc than non-haemolityc infection.  It showed thatvaccination with mixed (whole-cell and extracellular product)  vaccineof S. agalactiae â-haemolityc  was more effective to protect tilapia against Streptococcosis.
Karakteristik dan Patogenisitas Streptococcus Agalactiae Tipe ?-hemolitik dan Non-hemolitik pada Ikan Nila Esti Handayani Hardi; Sukenda -; Enang Harris; Angela Mariana Lusiastuti
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Streptococcus agalactiae was isolated from cultured Nile tilapia (Oreochromis niloticus) in Cirata gulfand Klaten. The isolates were Gram positive cocci, oxidative fermentative positive, motility, and catalasenegative, grown on media containing NaCl 6.5%, ?-haemolytic and non-haemolytic. Two types of S. agalactiae(?-haemolytic and non-haemolytic) are different from their variety of sugars fermentation. Strains ?-haemolytic can ferment more sugars, including arabinose, sorbitol, lactose, and trehalose. Experimentalinfectivity trials on Nile tilapia (size 15 g), non-haemolytic type showed more virulent. This type causedfaster mortality, more severe behavior changes, and pathology changes than â-haemolytic type. NonhemoliticS. agalactiae caused 48% mortality 6-24 hours after injection, whereas â-haemolitic type caused17% mortality which it occured in 48 hours after injection (mortality of fish control 2,22%). Behaviordisease signs caused by non-haemolitic S. agalactiae started to happen 6 hours after injection whereas 12hours in ?-haemolytic type infection. Histopatological changes were observed on fish eye, spleen, andbrain. Hyperaemia, hyperthrophi, degeneration, and necrosis were also found on infected fish. Thisresearch was concluded that non-haemolytic of S. agalactiae was more virulent than ?-haemolytic.
APLIKASI VAKSIN Streptococcus agalactiae UNTUK PENCEGAHAN PENYAKIT STREPTOCOCCOSIS PADA BUDIDAYA IKAN NILA (Oreochromis niloticus) Taukhid Taukhid; Angela Mariana Lusiastuti; Tuti Sumiati
BERITA BIOLOGI Vol 13, No 3 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i3.662

Abstract

The research with the aim to know the effectivity (yield gap) of the application of Streptococcus agalactiae vaccine (pure whole cell) in prevention of streptococcosis on tilapia (Oreochromis niloticus) culture has been carried out. The isolate of S.agalactiae – N14G was used as a master seed on vaccine production. Priming vaccination was administered by immersion method, and booster vaccination was taken th place two months latter by oral method. Challenge test at the lethal dose (LD50) against active bacteria was done at 14 days post booster vaccination, and observation was taken place for 14 days post artificial infection. The results of the research showed that the highest survival rate and relative percent survival (RPS) was found in group treated with Streptovac vaccine (S. agalactiae and A. hydrophila combination) (65.58% and 35.36%) followed by S. agalactiae vaccine (52.08% and 10.01%). The lowest survival rate was found in control group (46.75%). The result of confirmation effectivity of the vaccines by challenge test in the laboratory showed that the highest survival rate and relative percent survival (RPS) was found in S. agalactiae vaccine (50.00% dan 37.50%) followed by Streptovac vaccine (40.00% and 25.00%), and the lowest survival rate was found in control group (20.00%). Vaccination is better than the non vaccinated.
Efektivitas Pemberian Ekstrak Kipahit (Tithonia diversifolia) Sebagai Imunostimulan Untuk Pencegahan Motile Aeromonas Septicemia (MAS) Pada Ikan Patin (Pangasionodon hypophthalmus) Edwina Edwina; Mulyana Mulyana; Angela Mariana Lusiastuti
JURNAL MINA SAINS Vol. 3 No. 1 (2017)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (856.633 KB) | DOI: 10.30997/jms.v3i1.862

Abstract

Motile Aeromonas Septicemia (MAS) merupakan penyakit yang sering ditemui pada kegiatan budidaya, dan salah satu komoditas yang sering terserang oleh penyakit ini adalah ikan patin.  Penelitian ini bertujuan mendapatkan dosis terbaik dari simplisia tanaman kipahit untuk mencegah (MAS) pada ikan patin. Percobaan dilaksanakan tanggal 10 Januari 2014 sampai dengan 22 Maret 2014 bertempat di Balai Penelitian dan Pengembangan Budidaya Air Tawar, Sempur, Bogor. Ikan patin dipelihara selama 28 hari sebelum uji tantang dan 10 hari setelah uji tantang dengan perlakuan pakan menggunakan campuran simplisia kipahit dengan dosis 2,44%; 4,76%; 9,09%; dan 16,67%. Perlakuan kontrol adalah pakan komersil tanpa penambahan simplisia kipahit. Hasil penelitian menunjukkan bahwa pemberian pakan seluruh dosis perlakuan, baik sebelum maupun sesudah uji tantang tidak mempengaruhi nilai tingkat kelangsungan hidup, rasio konversi pakan, dan laju pertumbuhan spesifik ikan patin. Pemberian perlakuan setelah dilakukan uji tantang diduga memberikan pengaruh terhadap respon imun terhadap ikan patin, tetapi tidak memiliki aktivitas bakterisidal. Dosis simplisia kipahit terbaik untuk pencegahan MAS pada ikan patin adalah 16,67% karena pada dosis tersebut aktivitas imunostimulan bekerja lebih baik dibandingkan dosis yang lainnya, serta aman terhadap organ hati berdasarkan uji fungsi hati.Kata kunci : Ikan patin, kipahit, Motile Aeromonas Septicemia, pakan.
Pathogenicity test bacteria from Oreochromis niloticus and Clarias gariepinus aquaculture ponds Hardi, Esti Handayani; Halim, Aldi M.; Nugroho, Rudy Agung; Mawardi, Mira; Isnansetyo, Alim; Mariana Lusiastuti, Angela; Rahayu, Widianingsih; Niklani, Andi; Saptiani, Gina
Jurnal Akuakultur Indonesia Vol. 22 No. 1 (2023): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.22.1.97-105

Abstract

Present research aimed to examine the pathogenicity opportunities of some bacteria which were isolated from freshwater aquaculture ponds to some fish Oreochromis niloticus, Clarias gariepinus, and Pangasius pangasius. The bacteria were isolated from the water and feces of O. niloticus and C. gariepinus aquaculture in Samarinda, which were identified as Escherichia coli, Enterobacter cloacae, and Enterobacter amnigenus. An 0.1 mL-1 of each bacteria was intramuscular injection and evaluated the clinical signs, eksternal pathology, and fish mortality. The freshwater fish using in this research were Oreochromis niloticus, Clarias gariepinus, and Pangasius pangasius with size 10-15 g from Loa Kulu, Kutai Kartanegara Regency, East Kalimantan, Indonesia. The result showed that each bacteria caused mortality in fish: E. coli bacteria caused fish mortality 23.33-66.67%; E. cloacae 10.00 to 90.00%; and E. amnigenus by 3.33-56.67%. The average death time of E. coli and E. amnigenus in all three fish were under 48 hours, while E. cloacae caused mortality less than 48 hours in C. gariepinus and P. pangasius. In conclusion, E. coli, E. cloacae, and E. amnigenus are putative pathogenic bacterium in O. niloticus, C. gariepinus, and P. pangasius.
Co-Authors ALIM ISNANSETYO Edwina Edwina Enang Harris Esti Handayani Hardi Gina Saptiani Halim, Aldi M. Hilma Putri Fidyandini Khairun Nissa Laksono Trisnantoro M. Zairin Junior Mira Mawardi Mulyana Mulyana Niklani, Andi Rahayu, Widianingsih Rudy Agung Nugroho Sri Nuryati Sukenda . Sukenda . Taukhid Taukhid Tuti Sumiati