Article Per Year (5 Year)
p-Index From 2021 - 2026
2.386
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Jurnal Sumberdaya Hayati Jurnal Biologi Tropis Jurnal Pusat Inovasi Masyarakat Makara Journal of Science Jurnal Pengabdian Masyarakat Bangsa Berita Biologi
Sri Listiyowati
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary

Published : 19 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 19 Documents
Search

 1   2 
Pengenalan dan Pengembangan Produk Dari Jamur Liar Edible Di Sukabumi, Jawa Barat Ivan Permana Putra; Sri Listiyowati; Elis Nina Herliyana; Muhammad Iqbal Nurulhaq
Jurnal Pengabdian Masyarakat Bangsa Vol. 1 No. 8 (2023): Oktober
Publisher : Amirul Bangun Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59837/jpmba.v1i8.388

Abstract

Berdasarkan hasil observasi awal melalui komunikasi kami dengan penduduk usia produktif sekitar kawasan Goalpara, Sukabumi, Jawa Barat, Desa ini merupakan salah satu lokasi yang memiliki pengetahuan lokal mengenai koleksi, preservasi, dan konsumsi jamur liar edible (JLE). Namun pengetahuan tersebut hanya dimiliki oleh sebagian kecil masyarakat yang berada di sana sehingga perlu untuk diwariskan ke generasi berikutnya. JLE sangat berpotensi untuk digunakan sebagai salah satu alternatif pemenuhan kebutuhan pangan karena jumlahnya banyak, mudah ditemukan, dan telah dilaporkan memiliki berbagai macam manfaat dan nutrisi yang baik. Selain itu, Kelompok perambah JLE di desa tersebut belum memiliki keterampilan untuk mengolah JLE menjadi produk turunannya untuk memberikan nilai tambah serta menyimpannya dalam jangka waktu tertentu. Tujuan dari kegiatan pengabdian ini adalah untuk mengenalkan keragaman LE kepada mitra dan melakukan pendampingan pembuatan produk dari JLE. Metode yang dilakukan meliputi inventarisasi dan validasi identitas taksonomi JLE, sosialisasi dan diseminasi potensi JLE dan cara merambah jamur yang aman, serta pelatihan sederhana preservasi dan pembuatan olahan JLE. Hasil kegiatan pengabdian menunjukkan sebanyak 3 spesies JLE yang bisa ditemukan di sekitar lokasi pengabdian yaitu: supa jambu (Armillaria nova-zelandiae), jamur kuping liar (Auricularia delicata), dan jamur tiram phoenix (Pleurotus pulmonarius). Pada kegiatan pengabdian ini juga telah dibuat beberapa produk diantaranya adalah: kripik jamur liar, jamur tiram krispi, dan jamur kuping kering. Hasil dari kegiatan pengabdian ini diharapkan mampu menjadi alternatif pemenuhan bahan pangan masyarakat dan penambahan nilai dari JLE.
Antagonistic Mechanism of Entomopathogenic Fungi Against Fusarium oxysporum f. sp. cubense, The Causal Agents of Banana’s Panama Disease Listiyowati, Sri; Rustiani, Tya; Rahayu, Gayuh
Jurnal Fitopatologi Indonesia Vol 19 No 3 (2023): Mei 2023
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.3.99-110

Abstract

Fusarium oxysporum f. sp. cubense merupakan cendawan tular tanah penyebab penyakit panama pada tanaman pisang. Agens hayati dari kelompok cendawan telah banyak dilakukan untuk mengendalikan penyakit tanaman. Oleh sebab itu, penelitian ini bertujuan mengidentifikasi empat koleksi cendawan entomopatogen berdasarkan pada ciri morfologinya dan mengevaluasi mekanisme antagonismenya terhadap F. oxysporum f. sp. cubense IPBCC 19 1472. Galur cendawan entomopatogen dengan kode PS 4, PS 9, PS 11 berasal dari kawasan Situ Gunung, Sukabumi, Jawa Barat dan galur KRC berasal dari Kebun Raya Cibodas, Jawa Barat. Identifikasi cendawan dilakukan berdasarkan ciri morfologi pada medium agar-agar dekstrosa kentang. Mekanisme antagonistik diteliti menggunakan metode biakan ganda dan sebagai kontrol digunakan biakan tunggal F. oxysporum f. sp. cubense. Pengamatan dilakukan terhadap daya hambat cendawan entomopatogen dan pertumbuhan koloni F. oxysporum f. sp. cubense. Semua cendawan entomopatogen tidak dapat diidentifikasi secara morfologi karena tidak bersporulasi. Pertumbuhan koloninya lebih lambat daripada F. oxysporum f. sp. cubense. Semua cendawan entomopatogen menghambat F. oxysporum f. sp. cubense melalui mekanisme kompetisi ruang. Galur KRC memiliki aktivitas antagonisme paling besar, diikuti berturut-turut oleh galur PS 9, PS 11, dan PS 4. F. oxysporum f. sp. cubense membentuk klamidospora sebagai respons terhadap cendawan entomopatogen galur PS9 dan vakuolisasi ketika berinteraksi dengan tiga galur cendawan entomopatogen lainnya.
Bioethanol Production from Non-Conventional Yeasts Wickerhamomyces anomalus (Pichia anomala) and Detection of ADH1 Gene Fathiah, Muhammad Fadhil; Hartono, Faisal Diniamal; Astuti, Rika Indri; Listiyowati, Sri; Meryandini, Anja
HAYATI Journal of Biosciences Vol. 31 No. 2 (2024): March 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.2.221-228

Abstract

Bioethanol is an organic compound resulted from the fermentation of sugar substrates by microorganisms which is used as alternative energy sources. During bioethanol fermentation yeast are exposed to various fermentation stresses, including temperature, osmotic, and oxidative stresess. Such conditions may decrease ethanol production. We previously isolated fermentation-stress tolerance yeast isolates from traditional Balinese beverages, identified as Wickerhamomyces anomalus BT2, BT5, and BT6. However no data available regarding the bioethanol production of those isolates. Our study indicates that these strains could utilize various sugar substrates (glucose, xylose, maltose, sucrose) in oxidative fermentative media. The highest value of substrate utilization efficiency following 48 hours fermentation was shown by BT6 on glucose (61.02%), BT 2 on xylose (55.44%) and maltose (60.90%). Measurement of ethanol production by Gas Chromatography showed that the strains were able to produce higher ethanol on the glucose substrate than other substrates. For instance, BT6 could produce the highest ethanol production (5.00 g/L) amongst strains tested by using glucose as substrate. Yet, the particular strains could only produce 0.30 g/L and 0.65 g/L by using xylose and maltose, respectively. For further genetic engineering purposes, we detected ADH1 gene from all three isolates, with high homology to the alcohol dehydrogenase from Saccharomyces cerevisiae, Geobacillus stearothermophilus and Pseudomonas aeruginosa. Further strain development can be carried out targeting the ADH1 gene, important for ethanol fermentation.
Karakterisasi dan Potensi Acremonium sp. Secara In Vitro Terhadap Cendawan Patogen Fusarium oxysporum: Characterization and Potential of Acremonium sp. Against Pathogenic Fungi Fusarium oxysporum In Vitro Listiyowati, Sri; Irvanto, David
Jurnal Sumberdaya Hayati Vol. 10 No. 2 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.2.108-113

Abstract

Various natural interactions exist between fungi and plants, including mutualism and pathogenesis. This study evaluated the interaction of fungi Acremonium sp. to plants and pathogenic fungi. Acremonium sp. was isolated from the root tissue of an oil palm plant using surface sterilization methods before isolation. The potential pathogenicity interaction of Acremonium sp. was assayed towards Chinese white cabbage (Brassica rapa L. var. pekinensis) in vitro. Based on the PDA medium assay, isolated Acremonium sp. exhibited pathogenic traits against Chinese white cabbage within 14 days post-inoculation. Interestingly, Acremonium sp. showed antifungal activity against Fusarium oxysporum IPBCC.19.1468, with an average inhibition rate of 69.11%. Our data suggest the pathogenicity interaction of Acremonium sp. toward cabbage plant and fungi F. oxysporum IPBCC.19.1468.
Isolasi dan Identifikasi Cendawan Endofit Akar Anggrek Epifit Vanda sp. dan Anggrek Terestrial Spathoglottis plicata: Isolation and Identification of Root Endophytic Fungi in Epiphytic orchid Vanda sp. and Terrestrial Orchid Spathoglottis plicata Rahayu, Nadiya Dwi; Listiyowati, Sri; Rafi, Mohamad; Sandra, Edhi; Mursidawati, Sofi; Risna; Sukarno, Nampiah
Jurnal Sumberdaya Hayati Vol. 10 No. 4 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.4.198-204

Abstract

Endophytic fungi play an important role in the orchid lifecycle by aiding seed germination and plant development. Information on the diversity of endophytic fungi in Indonesian orchids, particularly Vanda epiphytic orchid and Spathoglottis terrestrial orchid, is still limited. This study aimed to isolate and identify endophytic fungi from the roots of Vanda sp. and Spathoglottis plicata orchids collected from Banjarbaru, South Kalimantan, and Kendari, Southeast Sulawesi. Isolation was performed using Potato Sucrose Agar (PSA) medium, and morphological identification was carried out through macroscopic and microscopic observations. A total of four endophytic fungal isolates were identified, including Nigrospora sp. and Penicillium sp. from Vanda sp. roots, and two isolates of Fusarium sp. from Spathoglottis plicata roots. The four fungal isolates obtained had aerial mycelium and septate hyphae, as well as conidia with diverse shapes. The diversity of endophytic fungi in orchid species could be influenced by the fungal affinity on the specific microhabitat conditions of each tissue and host plant species.
Morphological and Molecular Identification of Culturable Arbuscular Mycorrhizal Fungi (AMF) Associated with Pternandra azurea from Martabe Batang Toru Forest, North Sumatra, Indonesia Maulani, Nail Izzatul; Sukarno, Nampiah; Yulandi, Adi; Listiyowati, Sri; Kramadibrata, Kartini; Subagya, Mahmud; Anwar, Syaiful
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1240-1250

Abstract

Arbuscular Mycorrhizal Fungi (AMF) form a mutualistic symbiosis with almost all host plant species and exist in various ecosystems. Studies of AMF diversity in Sumatra tropical rainforest plants are still limited. This research aimed to isolate and identify AMF associated with Pternandra azurea from Martabe Batang Toru forest, North Sumatra. The spores were obtained from the rhizosphere of P. azurea and propagated by trap culture using Pueraria javanica, Sorghum vulgare, and Zea mays. AMF spores were isolated using wet sieving and decanting techniques, then inoculated into roots of P. javanica grown in sterile zeolite to obtain a single-species culture. The AMF were identified based on spore morphology and molecular analysis using AML1/AML2 specific primers. A total of 13 AMF single-species cultures were obtained, and based on morphological characteristics, they were identified as Claroideoglomus lamellosum, C. claroideum, Acaulospora rehmii, A. longula, and Glomus ambisporum. Further identification using molecular analysis, the cultures were identified as C. etunicatum, A. spinosa, A. longula, and G. ambisporum. Molecular identification resulted in different AMF species from morphological identification. Claroideoglomus was dominant AMF observed. All cultures formed internal hyphae, arbuscules, and vesicles within the roots. The AMF cultures obtained can be used as biofertilizers to restore degraded ecosystems.
Pendampingan Pembuatan Herbal Mineral Blok sebagai Suplemen Tambahan Ternak Ruminansia di Desa Tawangrejo Widyaningrum, Dyah Puspa; Natasya, Dhea; Raihan, Raka; Quddusy, Fairuz Nabil; Rosalia, Annisa Wyne; Arum, Dewi; Hafizhoh, Putri Hasna; Syahidah, Hilyah; Listiyowati, Sri
Jurnal Pusat Inovasi Masyarakat Vol. 7 No. 1 (2025): April 2025
Publisher : Direktorat Pengembangan Masyarakat Agromaritim, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpim.7.1.25-33

Abstract

The village of Tawangrejo possesses several potential developments, particularly in the livestock sector, but the maintenance of livestock generally relies on forage without additional mineral supplements. Therefore, assistance is necessary to facilitate the production of Herbal Mineral Block (HMB) supplements for the breeders. This program is designed to impart knowledge and skills on producing additional mineral supplements for ruminant livestock feed to enable breeders to produce HMB supplements independently. The methods used were socialization, distribution of HMB informational leaflets, hands-on demonstrations of HMB production, and pre-test and post-test evaluations to measure the program's success. The results of the assessment of changes in conditions before and after the program showed that there was an increase in the knowledge, attitudes, and breeders’ skills of Tawangrejo. In the high category, the knowledge aspect increased from 14.3% to 100%, the attitude aspect increased from 57.1% to 100%, and the skills aspect increased from 4.8% to 95.2%. Through this program, Tawangrejo breeders are assessed as having an understanding of additional mineral supplements as important for livestock health and having the skills to make HMB independently. Adding HMB supplements to livestock can increase productivity, nutrient absorption, and body weight.
A CONTROL OF OXYGEN SUPPLY IN BIOCONVERSION OF SUGARCANE TRASH INTO XYLITOL BY Meyerozyma guilliermondii InaCCY65 Thontowi, Ahmad; Kanti, Atit; Listiyowati, Sri; Meryandini, Anja
Berita Biologi Vol 23 No 3 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2024.7720

Abstract

Xylitol is a sugar alcohol used as a sugar substitute for several prevention of health cases such as dental diseases, diabetes, and other health problems. Bioconversion of xylose into xylitol needs an optimum oxygen supply for xylitol synthesis. This research aims to determine the effect of dissolved oxygen on hydrolysate fermentation from sugar cane leaf as a source of xylose to xylitol by Meyerozyma guilliermondii InaCCY65. Dissolved oxygen was varied in aeration and fermentation agitation using a 3L scale bioreactor. Analysis of cell growth for several kinetic parameters during fermentation, xylose reductase, and xylitol dehydrogenase activity. Bioconversion of sugarcane trash hydrolysate into xylitol by M. guilliermondii InaCCY65 is influenced by the oxygen transfer coefficient (kLa) and aeration conditions. The increase in kLa number showed increased cell growth, xylose consumption, xylitol production, and decreased InaCCY65 cells. The optimum conditions of kLa were obtained at 45/h with 39 g/L xylitol production under the aeration effect. Optimum aeration in the bioconversion of sugarcane trash (SCT) hydrolysate become xylitol by M. guilliermondii InaCCY65 is 1.0%; under these conditions, xylitol yield and xylitol productivity are 0.78 g/g and 1.20 g/Lh. The effect of oxygen in the bioconversion of xylose to xylitol also has an impact on xylose reductase (XR) and xylose dehydrogenase (XDH) activities of M. guiellermondii InaCCY65. The results showed that the dissolved oxygen concentration must be carefully controlled during xylitol bioconversion to obtain efficient xylitol.
IDENTIFICATION OF Aspergillus flavus AND DETECTION OF ITS AFLATOXIN GENES ISOLATED FROM PEANUT AND PEANUT PROCESSED PRODUCTS Nagur, Kemala S.; Sukarno, Nampiah; Listiyowati, Sri
BIOTROPIA Vol. 21 No. 1 (2014): BIOTROPIA Vol. 21 No. 1 June 2014
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.864 KB) | DOI: 10.11598/btb.2014.21.1.398

Abstract

Aspergillus flavus is one of the main fungi that are able to produce aflatoxin.The presence of the fungi and its aflatoxin are become serious problem on food safety. This research was aimed to isolate and identify A. flavus from peanut and its processed products collected from some traditional markets in Bogor, Depok and Jakarta, and detection of their aflatoxin genes. Fungal isolation was using AFPA media. Fungal identification was carried out by combining morphological and molecular analysis used species specific primers FVAVIQ1/FLAQ2 and AFLA-F/AFLA-R, while detection of  aflatoxin genes employed four specific primers of apa-2 (aflR), nor-1 (aflD), ver-1 (aflM) and omt-1 (aflP). From 36 samples, the A. flavus group was only found in peanut kernels samples with viable count of specific colonies in the range of 0.01-5.52 x 10-4 cfu/g. The total 18 isolates were identified as A. flavus based on species specific primers FVAVIQ1/FLAQ2 and AFLA-F/AFLA-R by producing amplicons about 100 and 413 bp respectively. Based on aflatoxin gene analysis showed that all 18 isolates successfully amplified by both apa-2 and nor-1, 83.3 % by omt-1 and 72.2 % by ver-1 genes which taking part in aflatoxin production. The amplicons size of apa-2, nor-1, ver-1 and omt-1   primer pairs were about 1032, 400, 895 and 1024 bp respectively.
Co-Authors ADI YULANDI Ahmad Thontowi ALEX HARTANA Alex Hartana Anja Meryandini Arum, Dewi Astri Ariyani Atit Kanti Cici Rahmawati Delfiani Anggias Putri Edhi Sandra Elis Nina Herliyana Fathiah, Muhammad Fadhil Firda Sri Efendi Firyal Noviatanti Nabilah Gayuh Rahayu Hafizhoh, Putri Hasna Handika Dwi Prasetyo Hartono, Faisal Diniamal Ira Zahara Nurwulansari Irmanida Batubara IRVANTO, David Ivan Permana Putra Kartini Kramadibrata Kemala S. Nagur Lenka, Audria Bayu M. Rafi Maulani, Nail Izzatul Maulida Aqlinia MUHAMMAD JUSUF Muhammad Jusuf Nadila Fany Ashafani Nampiah Sukarno Natasya, Dhea Nurulhaq, Muhammad Iqbal Okky Setyawati Dharmaputra Quddusy, Fairuz Nabil Rahayu, Nadiya Dwi Raihan, Raka Rika Indri Astuti Risna Rosalia, Annisa Wyne Rustiani, Tya Sofi Mursidawati Subagya, Mahmud Syahidah, Hilyah Syaiful Anwar UTUT WIDYASTUTI UTUT WIDYASTUTI Vincentia Fenice Angger Maherani Wahyu Aji Mahardhika Wendi Nurul Fadillah Widyaningrum, Dyah Puspa Yanti Novera