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Resistance Detection of Aedes aegypti Larvae To Cypermethrin from Endemic Area In Cimahi City West Java Endang Puji Astuti; Mara Ipa; Firda Yanuar Pradani
ASPIRATOR - Journal of Vector-borne Disease Studies Vol 6 No 1 (2014): Jurnal Aspirator Volume 6 Nomor 1 2014
Publisher : Loka Litbang Kesehatan Pangandaran

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Abstract. Vector control programs using chemical insecticide e.g organochlorin, organophosphate, carbamate, and pyrethroid (cypermethrin). When those insecticides were applied continuously, it may lead to vector resistance. The aim of this research was to detect any resistance of Ae. aegypti to cypermethrin in endemic areas of Cimahi. This research is a laboratory study that used biochemical test which referred to Lee’s method. Larva samples were collected from 8 villages, which are endemic area. Samples of larvae were collected from 15 villages belonged to dengue endemic areas in town of Cimahi, however, villages that meet the availability of larvae were only 8 villages. To detect the activity of monooxygenase enzyme, a biochemical assay was used in this research by created a reaction between larvae homogenate and sodium acetate substrate. The results of reaction were read using ELISA reader with spectrophotometer wave length of 595 nm. Overall, the results showed that most of the larvae in eight villages of Cimahi is still susceptible to cypermethrin. However, larvae from Cibabat village were 4% resistant, 2% tolerant, and 94% susceptible. On the other hand, Cigugur village showed that 12.7% larvae were tolerant and 87.3% still susceptible. Other villages like Cimahi, Cibeureum, Melong, Baros, Cipageran, and Pasirkaliki still remains susceptible. Resistance detection using biochemical assay of cypermethrin insecticide for Ae.aegypti resulting data stated that in 6 villages were still susceptible but in 3 other villages were already tolerant and 1 village was already resistance.
KONFIRMASI ANOPHELES BARBIROSTRIS SEBAGAI VEKTOR MALARIA DI WAIKABUBAK MELALUI DETEKSI PROTEIN CIRCUM SPOROZOITE Mara Ipa; Heni Prasetyowati; Yuneu Yuliasih
ASPIRATOR - Journal of Vector-borne Disease Studies Vol 4 No 1 (2012): Jurnal Aspirator Volume 4 Nomor 1 2012
Publisher : Loka Litbang Kesehatan Pangandaran

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Abstrak. Nyamuk Anopheles spp. dinyatakan sebagai vektor malaria apabila ditemukan sporozoitdi kelenjar ludahnya dan salah satu metode yang dapat dilakukan adalah melalui uji Enzyme-LinkedImmunosorbent Assay (ELISA). Penelitian ini dilakukan untuk mendeteksi protein circum sporozoite padatersangka vektor malaria Anopheles barbirostris melalui metode ELISA. Penelitian ini dilakukan di daerahendemis malaria di Desa Modu Waimaringu, Kecamatan Waikabubak, Kabupaten Sumba Barat pada bulanMaret 2011. Desain studi penelitian ini adalah cross sectional, nyamuk uji diperoleh melalui penangkapannyamuk sekitar kandang. Uji ELISA dilakukan pada bagian kepala dan dada nyamuk An. barbirostrisyang potensial mengandung sporozoit Plasmodium falciparum dan Plasmodium vivax. Hasil penelitianmenunjukkan dari 40 sampel An. barbirostris yang diuji di Desa Modu Waimaringu seluruhnya negatif(100%). Hal ini berarti tidak ditemukannya protein circum sporozoite dan An.barbirostris bukan vektorpada daerah tersebut.
Status Resistensi Aedes aegypti dengan Metode Susceptibility di Kota Cimahi terhadap Cypermethrin Firda Yanuar Pradani; Mara Ipa; Rina marina; Yuneu Yuliasih
ASPIRATOR - Journal of Vector-borne Disease Studies Vol 3 No 1 (2011): Jurnal Aspirator Volume 3 Nomor 1 2011
Publisher : Loka Litbang Kesehatan Pangandaran

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Abstracts: Vector control of dengue usually doing by using insecticides, whether by governmentor insecticides used in household. Using to much insecticides for long time can causedresistence of mosquito. This research aim to know resistance status of Aedes aegypti fromendemic rural in district Cimahi to cypermethrin (synthetic pyretroid). Resistance statusknowing by susceptibility methods (WHO standard) with using impregnated paper that containingcypermethrin 0.2% and 0.4%. Aedes aegypti spread by it for 15, 30, 45 and 60minutes. Number of mortality count by percentage death mosquito in each time and eachconcentration of cypermethrin. Data interpreted by WHO standard, which percentage ofdeath mosquito <80% is resistance, between 80-98% is tolerance and 99-100% is susceptible.Aedes aegypti from endemic rural in district Cimahi showed resistance of cypermethrin0.2% and 0.4%. This result showed that all mosquito still alive after 15 minutes spreadingby cypermethrin 0.2%, and only 6.7% mosquito death in cypermethrin 0.4%. After 30minutes, death mosquito counted 46.7% in cypermethrin 0.2% and 73.3% in 0.4%. 46.7%mosquito was death in 45 and 60 minutes spreading by cypermethrin 0.2% and 73.3% in0.4%. The result showed resistance ratio (RR50) of mosquito is 4.6. Aedes aegypti from endemicrural in district Cimahi showed a resistance to cypermethrin 0.2% and 0.4%.
Co-Authors Aryo Ginanjar Endang Puji Astuti Firda Yanuar Pradani Heni Prasetyowati Joni Hendri Rina Marina Yuneu Yuliasih