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All Journal Folia Medica Indonesiana Jurnal Media Gizi Indonesia (MGI) The Indonesian Biomedical Journal Indonesian Journal of Cardiology Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) JOURNAL OF COMMUNITY MEDICINE AND PUBLIC HEALTH RESEARCH Cardiovascular and Cardiometabolic Journal (CCJ) Journal of Global Pharma Technology
Budi Susetyo Pikir
Departemen Kardiologi Dan Kedokteran Vaskuler, Fakultas Kedokteran –RSUD Dr. Soetomo. Kelompok Peneliti Sel Punca – Institut Penyakit Tropik, Universitas Airlangga, Surabaya
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Other

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Gene Expression of SOX2, OCT4, and Nanog by Small Molecule Compound VC6TFZ on Peripheral Blood Mononuclear Cell Rizka Amalia; Budi Susetyo Pikir; Andrianto Andrianto
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 28 No. 2 (2022)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v28i2.1759

Abstract

Peripheral blood mononuclear cells are a potential source of cells to be induced into pluripotent stem cells because the collection procedure is easy, minimally invasive, and can be stored in a frozen form. Small molecule compound VC6TFZ consisting of valproic acid (VPA), CHIR990210 (CHIR), 616452, Tranylcypromine, Farsokline, 3-deazaneplanocin (DZnep) and TTNPB has been shown to induce pluripotency in mouse fibroblasts, but this has not been proven in peripheral blood cells. This chemical reprogramming strategy has the potential to be used in producing the desired functional cell types for clinical applications. This study aims to determine whether the small molecule compound VC6TFZ can induce pluripotency of peripheral blood mononuclear cells to become induced pluripotent stem cells. Mononuclear cells were isolated from peripheral venous blood by density gradient centrifugation method. The cells are grouped into 4 groups. Group 1 was the control group, which was not exposed to the small molecule. Groups 2-4 were experimental groups exposed to different doses of the small molecule VC6TFZ. Identification of induced pluripotent stem cells was carried out by identifying colony morphology and pluripotent gene expression of Octamer-binding transcription factor-4 (OCT4), Sex-determining region Y-box 2 (SOX2), and Nanog using Real-Time Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR). Colonies with a round shape, large, cobble stone like, and clear boundaries resembling pluripotent stem cell colonies appeared on the 9th day of the induction process. OCT4 and Nanog gene expression were significantly increased in the treatment group compared to the control.
The Relationship Between Energy Drinks A and B with Blood Pressure and Pulse Rate Increase Among College Students Setiabudi, Narendra Arya; Pikir, Budi Susetyo; Lestari, Pudji; Purwanto, Bambang
Journal of Community Medicine and Public Health Research Vol. 5 No. 1 (2024): Journal Community Medicine and Public Health Research
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jcmphr.v5i1.52676

Abstract

Energy drinks are popular among various groups of people, such as teenagers and working individuals. These beverages are consumed to provide additional energy and improve focus. This research investigates the relationship between energy drinks and blood pressure and heart rate in students of the Faculty of Medicine at Universitas Airlangga, Surabaya, Indonesia. The study used the open trial – randomized cross-over study method and collected primary data through experiments. The sample consisted of students from the Faculty of Medicine at Universitas Airlangga, aged 19 to 22 years, with a normal body mass index ranging from 18.5 to 24.9. The systolic, diastolic, and heart rate values were measured before and after consuming energy drinks. A total of 38 research samples were used, including 22 males (57.8%) and 16 females (42.1%). Energy drinks such as Kratingdaeng and Extra Joss showed a significant increase in blood pressure. However, no significant changes were observed in heart rate. The results of the Wilcoxon statistical test, comparing systolic and diastolic conditions before and after consuming Extra Joss, showed p<0.0001. A significance level of p<0.05 indicates a significant effect of energy drinks on the students. The Wilcoxon test for Kratingdaeng also yielded a p<0.0001. For the p-value through the Wilcoxon test on diastolic values before and after consuming Extra Joss and Kratingdaeng, the result was p<0.05. However, the Wilcoxon test for heart rate before and after consuming Kratingdaeng and Extra Joss had a at p<0.05. In the Mann-Whitney statistical test, a p<0.05 was found only in the post-systolic condition, while other conditions had at p≥0.05. In conclusion, energy drinks significantly affected blood pressure, but no significant changes were observed in heart rate.
Co-Authors Andrianto Annisa Trissatharra Bambang Purwanto Budi Bakti Dharmadjati David Setyo Budi Fita Triastuti Hendri Susilo Ilma Alfia Isaridha Imam Mahbub Zam Zami Irma Maghfirah Jannatin Nisa Arnindita Laurentius Andre Mochamad Yusuf Alsagaff MOCHAMMAD THAHA Mochammad Yusuf Alsagaff Mohammad Satya Bhisma Pakpahan, Cennikon Pudji Lestari Rendra Mahardhika Putra Rizka Amalia Setiabudi, Narendra Arya Sinta Dwi Juniar Suryantoro, Satriyo Dwi Wungu, Citrawati Dyah Kencono