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All Journal BALI MEDICAL JOURNAL (BMJ) The Indonesian Biomedical Journal AKSONA Neurona
Mohammad Hasan Machfoed
Departemen Neurologi FK Universitas Airlangga/RSUD Dr. Soetomo, Surabaya
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience Public Health

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Journal : The Indonesian Biomedical Journal

 1 
The Effect of Thymoquinone Administration on Local Immunoglobulin-G Levels of Rattus norvegicus Strain Wistar Sciatic Nerve Crush Injury Model Valentinus Besin; Abdul Hafid Bajamal; Mohammad Hasan Machfoed; Jusak Nugraha; Budi Utomo; Paulus Budiono Notopuro
The Indonesian Biomedical Journal Vol 14, No 1 (2022)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v14i1.1772

Abstract

BACKGROUND: Immunoglobulin-G (IgG) is a product of the initial response to secondary immune response, which accumulates in distal segment of the nerve after crush injury. Thymoquinone modulates the adaptive immune response. Effect of thymoquinone administration on local IgG levels of Rattus norvegicus Wistar rats sciatic nerve crush injury model has not been elucidated.METHODS: This was an experimental study, with 63 Rattus norvegicus Wistar rats that divided into 9 groups. Three groups were given placebo, 3 groups were given 100 mg/kg/day thymoquinone, and 3 groups were given 250 mg/kg/day thymoquinone. The rats were terminated based on the assigned group at 5x24, 6x24, and 7x24 hours and then the IgG levels were measured using sandwich enzyme-linked immunosorbent assay (ELISA).RESULTS: There was a significant difference in IgG levels after administration of 100 and 250 mg/kg/day thymoquinone at 5x24 hours and 7x24 hours post-injury compared to the rats that were given no treatment. A significant difference of IgG levels was also found after administration of 100 mg/kg/day thymoquinone group at 6x24 hours post-injury. Critical point of decreasing local IgG of all groups happened at 6x24 hours after injury, however, there was no significant difference in the median levels of thymoquinone at doses of 100 mg/kg and 250 mg/kg.CONCLUSION: Local IgG levels in distal segment of the sciatic nerve crush injury is lower in rats that were given 100 mg/kg thymoquinone treatment compared to the rats that receive no thymoquinone treatment since 5x24 hours after injury. Thymoquione administration should be given immediately after the crush injury until before 6x24 hours post-injury to decrease antibodies in degeneration process.KEYWORDS: thymoquinone, immunoglobulin-G, crush injury, sciatic nerve
Thymoquinone Modulates Local MMP-9, IL-10, and IgG in Sciatic Nerve Crush Injury Animal Model Valentinus Besin; Abdul Hafid Bajamal; I Ketut Sudiana; Mangestuti Agil; Jusak Nugraha; Mohammad Hasan Machfoed; Paulus Budiono Notopuro; Naesilla Naesilla
The Indonesian Biomedical Journal Vol 14, No 3 (2022)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v14i3.2011

Abstract

BACKGROUND: Interleukin (IL)-10 is involved in Wallerian degeneration after peripheral nerve crush injury. Oral thymoquinone was previously observed to decrease local immunoglobulin-G (IgG) in a crush-injured rat model. No study has evaluated the pathway of various thymoquinone dosages on local IgG and IL-10 in this injury.METHODS: This experimental study used 126 Rattus norvegicus Wistar rats that were divided into 18 groups: six groups received a placebo, the other six groups received thymoquinone at 100 mg/kg/day and the last six groups received thymoquinone at 250 mg/kg/day, respectively. Rats were sacrificed at 12, 18, 24, 5x24, 6x24, and 7x24 hours. Matrix metalloproteinase-9 (MMP-9), IL-10, and local IgG levels were assessed by Enzyme-Linked Immunosorbent Assay (ELISA). The nuclear factor KappaB (NF-κB) expressions on Schwann cells were examined by flow cytometry. Path analysis was performed using SmartPLS.RESULTS: The path analysis showed that 100mg/kg/day of thymoquinone significantly decreased NF-κB expression. However, NF-κB did not affect local MMP-9, and MMP-9 had no significant relationship with local IL-10 and IgG. Thymoquinone 250 mg/kg/day also significantly inhibited NF-kB expression, decreased local MMP-9, and, in turn, decreased local IL-10 and IgG.CONCLUSION:  Administration of oral thymoquinone 250 mg/kg/day decreases local IgG and IL-10 levels via suppressing NF-κB expression and MMP-9 levels.KEYWORDS: thymoquinone, crush injury, IgG, IL-10, MMP-9, NF-κB
Co-Authors A.A. Ketut Agung Cahyawan W Abdul Hafid B Andi Kurnia Bintang, Andi Kurnia Besin, Valentinus Budi Riyanto Wreksoatmodjo, Budi Riyanto Budi Utomo Chrismawan Ardianto Dede Gunawan, Dede Dion Andriawan Wisnujono Dodik Tugasworo Fritz Sumantri Usman, Fritz Sumantri H. Harsono, H. Hanik Badriyah Hidayati,* Mohammad Hasan Machfoed,* Kuntoro,** Soetojo,*** Budi Santoso,**** Suroto,***** Budi Utomo****** Jimmy Barus, Jimmy Junaidi Khotib Jusak Nugraha Kuntoro Kuntoro Kurniawan, Mohammad Mangestuti Agil Naesilla Naesilla Notopuro, Paulus Budiono Paulus Sugianto Riza Sulthan, Riza Rusdi Lamsudin, Rusdi S.Pd. M Kes I Ketut Sudiana .