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The Effect of Dietary Vitamin E (-Tocopherol) Level on the Lipid, Essensial Fatty Acid Level and the Hatching Rate of Catfish, Clarias batrachus Linn. Mokoginta, I.; Syahrizal, ,; Jr., M. Zairin
Jurnal Akuakultur Indonesia Vol. 1 No. 2 (2002): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This experiment was conducted to evaluate the dietary level of vitamin E (a-tocopherol) on the lipid, essential fatty acid level and the hatching rate of catfish, Clarias batrachus Linn. Five experimental diets with isoenergy and isonitrogen, but different vitamin E (a-tocopherol) level were used in this experiment. Dietary vitamin E levels are 18, 114, 210, 308 and 416 mg/kg diet respectively. Fish with body weight of 18,55 a 5,11 g per fish, fed on the experimental diets at satiation, for six months. The vitamin E, the lipid and the n-6 and n-3 fatty acid level of egg increase as the dietary vitamin E level of broodstock diet increase. However, the ratio of n-6/n-3 fatty acid of egg produced by treatment 210 mg vitamin E/kg diet was higher than that of other treatments, which finally produced the highest hatching rate of egg (p<0,05).Key words : Vitamin E (a-tocopherol), reproduction, catfish (Clarias batrachus Linn.)

The Effect of Enriched Daphnia sp. with Different Source of Oil on the Survival Rate and the Growth of Oreochromis niloticus Larvae Mokoginta, I.; Jusadi, Dedi; Pelawi, T.L.
Jurnal Akuakultur Indonesia Vol. 2 No. 1 (2003): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTThis experiment was conducted to evaluate best source of oil to enriched Daphnia sp. before fed it to Oreochromis niloticus larvae. Four treatments were used in this experiment; first, Daphnia sp. without encrichment, second Daphnia sp. enriched with fish oil, third Daphnia sp. enriched with corn oil and the fourth Daphnia sp. enriched with coconut oil. Three days old larvae fed on Daphnia sp. with size of £ 0,5 mm at the first week and 0,6 – 1,0 mm as the second week of this experiment. Larvae fed on Daphnia sp. 5 times daily, ad libitum, for 14 days. Larvae was reared in the small cages (2,25 l), and all cages was placed in the aquarium. Larvae density was 48 larvae/l. This experiment showed that the lipid level in Daphnia sp. enriched with oil was higher than that of no enrichment Daphnia sp. The highest n3- fatty acid level was found in Daphnia sp. enriched with fish oil, and the highest n6- fatty acid level was found in Daphnia sp. enriched with corn oil. Larvae fed on Daphnia sp. enriched with oil have a higher relative growth rate than that fed on Daphnia sp. without enrichment. The highest survival rate of larvae was found by feeding them with Daphnia sp. enriched with corn oil (p < 0,05).Key words : Daphnia sp., enrichment, larvae, Oreochromis niloticus ABSTRAKPenelitian ini dilakukan untuk mengevaluasi sumber minyak yang terbaik bagi pengkayaan Daphnia sp. sebelum diberikan ke larva ikan nila, Oreochromis niloticus. Ada 4 perlakuan dalam penelitian ini yaitu Daphnia sp. yang tidak diperkaya minyak; Daphnia sp. yang diperkaya minyak ikan; Daphnia sp. yang diperkaya minyak jagung; dan Daphnia sp. yang diperkaya minyak kelapa. Larva ikan nila, Oreochromis niloticus berumur 3 hari ditebar dalam hapa kecil (volume 2,25 l) sebanyak 48 ekor/l, dan seluruh hapa diletakkan dalam akuarium berukuran 100x50x40 cm. Daphnia sp. diperkaya terlebih dahulu dengan minyak sesuai perlakuan sebelum diberikan ke larva. Pada minggu pertama pemeliharaan larva, ukuran Daphnia sp. yang digunakan adalah £ 0,5 mm dan pada minggu ke dua 0.6 – 1.0 mm. Daphnia sp. diberikan sebanyak 5 kali dalam sehari secara ad libitum dan pemberian pakan dilakukan selama 14 hari. Hasil penelitian ini memperlihatkan bahwa kadar lemak Daphnia sp. yang diperkaya minyak lebih tinggi dari Daphnia sp. yang tidak diperkaya, dan hal ini berpengaruh pula pada kadar lemak tubuh larva. Kadar asam lemak –n3 tertinggi terdapat pada Daphnia sp. yang diperkaya minyak ikan dan kadar asam lemak –n6 tertinggi terdapat pada Daphnia sp. yang diperkaya minyak jagung. Larva yang diberi Daphnia sp. yang diperkaya dengan minyak mempunyai pertambahan bobot relatif dan tingkat kelangsungan hidup yang lebih tinggi dari larva yang diberi Daphnia sp. yang tidak diperkaya; dan tingkat kelangsungan hidup larva yang tertinggi dihasilkan oleh perlakuan pemberian Daphnia sp. yang diperkaya minyak jagung (P < 0,05), walaupun pertumbuhan bobot relatifnya sama dengan perlakuan lainnya (p > 0,05).Kata kunci : Daphnia sp., pengkayaan, larva, Oreochromis niloticus

The Improvement of Protein Retention through a Higher Utilization of Carbohydrate in the Diet with Chromium for Common Carp Cyprinus carpio Linn. Mokoginta, I.; Hapsyari, F.; Suprayudi, M. Agus
Jurnal Akuakultur Indonesia Vol. 3 No. 2 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This experiment was conducted to improve the protein retention through carbohydrate efficiency in the chromium diet of common carp Cyprinus carpio. Four experimental diets with isonitrogen and isocaloric but different Cr+3 level 0,00; 1,33; 2,23 and 3,96 ppm were used in this experiment. Fish with body weight of 5,31 ± 0,02 gram were reared in aquaria (50 x 40 x 35 cm); 20 fish per aquarium. Fish fed upon the experimental diets three times daily, at satiation for 50 days. Results of this experiment showed that the protein retention could be improved by given Cr+3 in the diet. Based on the respon curve of protein retention (Yi = -1.1678X2 + 3,7223X + 24,818 ; R2 = 0,9) it was found that the optimum Cr*J in the diet was 1,59 ppm. The maximum relative growth rate was found at 2,16 ppm Cr3+ (Y2 = -14,592 X: + 63,18IX + 426,43 ; R = 0,9). The feed efficiency and the survival rate of the fish are the same between treatment (p>0.05). The protein level of the fish from treatment 3,96 ppm Cr+3 was lower than the others; the lipid level of Cr+3 diets was higher than that of non Cr+3 diet; however the carbohydrate level of fish from diet 2,23 Cr+3 was lower than the others. It was concluded that the optimum level of Cr +3 in the diet of common carp was 1,59-2,16 ppm Key words : dietary chromium, common carp, Cyprinus carpio ABSTRAK Penelitian ini bertujuan untuk meningkatkan retensi protein dan pertumbuhan ikan mas Cyprinus carpio melalui peningkatan efisiensi karbohidrat pakan yang diberi kromium (Cr"3). Empat macam pakan dengan kadar Cr+3 yang berbeda yaitu 0,00; 1,33; 2,23 dan 3,96 ppm tetapi kadar protein dan energi yang sama digunakan pada penelitian ini. Ikan mas berbobot 5,31 ± 0,02 gram per ekor dipelihara dalam akuarium (50x40x35 cm) dengan kepadatan 20 ekor. Ikan diberi pakan tiga kali sehari secara at satiation. Pemberian pakan dilakukan selama 50 hari. Hasil penelitian memperlihatkan bahwa adanya pemberian Cr+3 dalam pakan akan mengefisienkan penggunaan karbohidrat pakan sehingga retensi protein akan meningkat pula. Retensi protein tertinggi dicapai pada kadar Cr+3 1,59 ppm (dihitung dari persamaan y, = -1,1678X2 + 3,7223X + 24,818; R2 = 0,9). Pertumbuhan relatif tertinggi dicapai pada kadar Cr+3 2,16 ppm (dihitung dari y2 = -14,592 X2 + 63.181X + 426,43; R2 = 0,9); sedangkan efiseinsi pakan dan tingkat kelangsungan hidup antar perlakuan sama (p>0,05). Kadar protein tubuh perlakuan 3,96 ppm Cr+3 relatif lebih rendah dari perlakuan lainnya. Kadar lemak tubuh ikan yang diberi Cr+3 lebih tinggi dari yang tidak diberi Cr+3; sedangkan kadar karbohidrat tubuh terendah pada perlakuan 2,23 ppm Cr+3. Secara keseluruhan dapat disimpulkan bahwa kadar Cr+3 optimum dalam pakan ikan adalah 1,59-2,16 ppm. Kata kunci : Kromium pakan. ikan mas, Cyprinus carpio

Effect of Different Feeding Method on Feed Conversion and Growth of Common Carp (Cyprinus carpio) in Floating Net Cage Utomo, N.B.P; Hasanah, P.; Mokoginta, I.
Jurnal Akuakultur Indonesia Vol. 4 No. 1 (2005): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This experiment was conducted to evaluate the effectiveness of two feeding methods commonly used in cage culture of carp Cyprinus carpio at Jatiluhur Lake. The results showed that the application of "at satiation feeding method" was more effective than "fixed method" (8% of body weight) indicating with food conversion ratio of 1.79 versus 1.84 and daily growth rate of 3.79% versus 3.42%. Keywords: common carp, Cyprinus carpio, FCR, at satiation, growth, cage culture ABSTRAK Penelitian ini dilakukan untuk mengevaluasi efektivitas dua metode pemberian pakan yang umum digunakan dalam budidaya keramba jaring apung untuk ikan mas di Waduk Jatiluhur. Hasil penelitian menunjukkan bahwa pemberian pakan secara satiasi lebih efektif dibandingkan dengan pemberian pakan sebanyak 8% dari bobot biomassa, dengan nilai konversi pakan sebesar 1,79 yang lebih kecil dibandingkan perlakuan pakan sebanyak 8% (1,84) dan pertumbuhan harian sebesar 3,79% (vs. 3,42%). Kata kunci: ikan mas, Cyprinus carpio, FCR, at satiation, pertumbuhan, keramba jaring apung

Effect of Different Feeding on Feed Conversion and Growth of Common Carp (Cyprinus carpio) in Floating Net Cage Culture at Jatiluhur Dike Utomo, N.B.P; Kumalasari, F.; Mokoginta, I.
Jurnal Akuakultur Indonesia Vol. 4 No. 1 (2005): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This experiment was conducted to evaluate the effectiveness of two feeding methods commonly used in cage culture of carp Cyprinus carpio at Jatiluhur Lake, Purwakarta. Common carp in mean weight of 24.29±4.29 gram were reared in floating net cage 7×7×3 m3, for 70 days rearing. Fish were fed on a commercial diet containing 30% protein at 5 times daily. Experimental treatment was feeding technique, i.e., by 6% of body weight, and at satiation for the second treatment. The results showed that the application of "at satiation feeding method" was more effective than "fixed method" (6% of body weight) indicating with food conversion ratio of 1.86 versus 1.91. Production of fish fed on the diet using at satiation method for 70 days was 1,241 kg/cage. Keywords: common carp, Cyprinus carpio, FCR, floating net cage, at satiation ABSTRAK Salah satu cara untuk menekan biaya dalam usaha budidaya ikan secara intensif adalah dengan penggunaan pakan secera efisien agar ikan tumbuh optimal dan pakan yang terbuang seminimal mungkin. Penelitian dilakukan di Waduk Jatiluhur, Purwakarta. Ikan mas (Cyprinus carpio) ukuran bobot awal rata-rata 24,29±4,29 gram dipelihara dalam jaring apung ukuran 7×7×3 m3, selama 70 hari. Ikan diberi pakan dengan frekuensi yang sama sebanyak 5 kali/hari. Perlakuan pada penelitian ini adalah teknik pemberian pakan, yaitu ikan pada jaring pertama diberi pakan sebanyak 6% dari bobot biomassa, sementara pada jaring kedua ikan diberi pakan sekenyangnya (at satiation). Hasil penelitian menunjukkan bahwa pemberian pakan dengan metode sekenyangnya (at satiation) menghasilkan nilai FCR sebesar 1,86 yang relatif lebih efisien dibandingkan dengan sebanyak 6% berdasarkan bobot biomassa (1,91). Produktivitas akhir ikan dengan pemberian pakan sekenyangnya 70 hari pemeliharaan dalam jaring apung di waduk Jatiluhur mencapai 1.241 kg. Kata kunci: ikan mas, Cyprinus carpio, FCR, Keramba jaring apung, at satiation

THE EFFECT OF DIFFERENT LEVELS OF VITAMIN E ON THE REPRODUCTIVE PERFORMANCE OF ZEBRAFISH (Danio rerio) Utomo, N.B.P; Junior, M. Zairin; Yusuf, T.L.; Mokoginta, I.; Bintang, M.
Jurnal Akuakultur Indonesia Vol. 4 No. 2 (2005): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This experiment was conducted to determine the dietary Vitamin E requirement for reproduction of broodstock zebrafish Danio rerio. Four isonitrogenous (39% crude protein) and isocaloric (3,260 kcal digestible energy/kg diet) practical diets, namely diets A, B, C, and D with different levels of Vitamin E were fed to zebrafish broodstock. The broodstock were cultivated in aquaria. Diet A contained low dosage of Vitamin E (5 mg Vitamin E /kg diet), while diets B (62 mg Vitamin E /kg diet), C (125 mg Vitamin E /kg diet), and diet D (187 mg Vitamin E /kg diet), combined respectively with 1% n-3 fatty acids and 2% n-6 fatty acids. Fish were fed ad satiation for 60 days using these diets. During feeding period, gonad maturation stages were examined. The dietary with different level of Vitamin E affected egg size, chemical content, total number of larvae, normal larvae, and survival rate of larvae produced, fecundity, fertilization rate, and hatching rate. On the other hand, fish fed on experimental diets did not show any significance differences in the gonad somatic index, fecundity, fertilization rate, and hatching rate. Fish fed on diet D produced the highest fecundity (633.65 ± 71.86 eggs/grams of fish), fertilization rate (92.3 ± 7.7%), and hatching rate (80.31 ± 9.8%). The total lipid content of eggs were significant, ranging from D (39.7%), C (33.5%), B (32.1%), and A (29.5%). At a dosage of 1% of dietary n-3 fatty acids and 2% n-6 fatty acids, zebrafish require 187 mg Vitamin E/kg feed in the diet for reproduction. . Keywords: vitamin E, reproductive performance, Danio rerio ABSTRAK Penelitian ini ditujukan untuk mengetahui kebutuhan Vitamin E pada pakan induk ikan zebra (Danio rerio) untuk reproduksi. Empat pakan perlakuan yang iso-protein (39%) dan iso-kalori (3.260 kcal digestible energy/kg pakan), dinamakan pakan A, B, C, dan pakan D dengan kandungan Vitamin E yang berbeda diberikan kepada induk ikan zebra. Induk ikan dipelihara pada akuarium. Pakan A mengandung Vitamin E terendah (5 mg Vitamin E/kg pakan), sedangkan pakan B mengandung Vitamin E 62 mg Vitamin E/kg pakan, pakan C asam mengandung Vitamin E 125 mg /kg pakan, dan pakan D mengandung Vitamin E 187 mg /kg pakan. Semua pakan perlakuan mempunyai kombinasi asam lemak n-3 berbanding n-6 sebesar 1:2. Ikan diberi pakan secara ad satiation selama 60 hari pemeliharaan. Selama masa pemberian pakan, tingkat kematangan gonad diperiksa secara teratur. Perbedaan kandungan vitamin E dalam pakan mempengaruhi fekunditas, derajat pembuahan telur, serta derajat penetasan telur. Pakan perlakuan tidak mempengaruhi nilai gonad somatik indeks dan kelangsungan hidup larva umur 3 hari. Pemberian pakan D menghasilkan fekunditas (633.65 ± 71.86 telur/gram induk), derajat pembuahan telur (92.3 ± 7.7%), serta derajat penetasan telur (80.31 ± 9,8%) terbaik. Total kandungan lemak pada telur berbeda nyata, yaitu pada pakan D (39.7%), C(33.5%), B(32.1%), dan pakan A(29.5%). Pada kandungan 1% asam lemak n-3 dan 2% asam lemak n-6, ikan zebra membutuhkan 187 mg Vitamin E/kg pakan untuk reproduksi yang normal. Kata kunci: Vitamin E, penampilan reproduksi, Danio rerio

Effect of Enriched Feed by n-3 fatty acids and 2% of n-6 fatty acid on Danio rerio Reproduction Utomo, N.B.P; Nurmalia, L.; Mokoginta, I.
Jurnal Akuakultur Indonesia Vol. 4 No. 2 (2005): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This experiment was conducted to determine the optimum n-3 fatty acid level in the diet containing 2 % of n-6 fatty acid on the reproductive performance of zebra fish (Danio rerio). There experimental diets containing 0.0; 1.0; 1.5 % n-3 fatty acid with 2.0 % n-6 fatty acid was fed to the fish, three times daily, at satiation, for two months. In order to evaluate the gonadal development of the broodstock, two gonads og fish was used for histologis preparation in every 7 days. At the end of the second month, reproductive performance was evaluated through parameters of gonad somato indeks, fecundity, fertilization rate, hatching rate, yolk egg absorbtion rate, survival rate of 3 days old larvae. Sample of fish also was taken for proximate composition as the end of this experiment. Results shows that at the fifth weeks of this experiment, gonad of fish fed on 1.0 % of n-3 fatty acid and 2.0 % n-6 fatty acid already produce eggs with the some size, while others. Still produce small size of eggs. It was found also that the whole body of fish fed an diet with 1.0% n-3 fatty acid contain the highest protein level compare to two other diets. Based on the evaluation of reproduction performance parameters, it was concluded that the optimum dietary level of n-3 fatty acid with 2.0 % n-6 fatty acid for Danio rerio was 0.81 - 0.90 %. Keywords: essential fatty, acids, reproduction, zebra fish, Danio rerio ABSTRAK Penelitian ini bertujuan untuk menentukan kadar asam lemak n-3 optimum dalam pakan yang mempunyai kadar asam lemak n-6 tetap. Tiga macam pakan dengan kadar asam lemak n-3 berbeda yaitu 0.0; 1.0; dan 2.0 % diberikan pada ikan dengan bobot rata-rata 0.12 g. Pakan diberikan secara at satiation, 4 kali sehari selama 60 hari. Setiap 7 hari sekali diambil sampel ikan untuk pembentukan preparat histologi gonad dengan tujuan untuk mengevaluasi perkembangan gonad. Pada akhir penelitian, induk dipijahkan dan dievaluasi performan reproduksi berdasarkan parameter. Gonad Somato Indeks, fekunditas, derajat pembuahan, derajat penetasan dan kelangsungan hidup larva berumur 3 hari. Sampel tubuh ikan diakhir penelitian juga dievaluasi komposisi proksimatnya. Hasil penelitian ini memperlihatkan bahwa pada minggu ke-5 setelah pemberian pakan, gonad dari ikan yang diberi pakan asam lemak n-3 1.0 % mengandung telur-telur dengan ukuran yang seragam sedangkan ukuran telur ke dua perlakuan lainnya masih bervariasi. Kadar protein tertinggi juga terdapat pada tubuh ikan yang diberi pakan 1.0 % asam lemak n-3. Berdasarkan parameter performan reproduksi secara keseluruhan dapat disimpulkan bahwa kadar asam lemak n-3 optimum dalam pakan ikan zebra, Danio rerio adalah 0.81 - 0.90 %. Kata kunci: asam lemak, reproduksi, ikan zebra, Danio rerio

Effect of L-Ascorbyl-2-Phosphate Magnesium as a Vitamin C Source in Different Doses on Growth of Patin Pangasius Hypophthalmus Fingerlings Jusadi, D.; Dewantara, B.A.; Mokoginta, I.
Jurnal Akuakultur Indonesia Vol. 5 No. 1 (2006): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This study was aimed to determine optimum dose of L-Ascorbyl-2-Phosphate Magnesium in the diet of patin, Pangasius hypophthalmus fingerlings. Five isoprotein and isocaloric diets containing different dose of L-Ascorbyl-2-Phosphate Magnesium, i.e. 0, 25, 75 and 100 mg/kg diet were used in this experiment. Fish with an average size of 5.00±0.11 g were maintained at a density of 15 fish per aquarium. Fish were fed three times a day at satiation, for 40 days. The results of study shows that vitamin C content in fish body increased as the vitamin C level of the diet increased. Similar pattern to vitamin C content was also found in protein retention, lipid retention, daily growth rate, and feed efficiency (p< 0.05). Thus, it can be concluded that the most optimum dose of L-Ascorbyl-2-Phosphate Magnesium as vitamin C source for patin fingerlings in this experiment was 100 mg Vit C/kg diet. Higher dose of L-Ascorbyl-2-Phosphate Magnesium than 100 mg/kg diet however need to be further verified. Keywords: vitamin C, patin, Pangasius hypophthalmus. ABSTRAK Penelitian ini bertujuan untuk menentukan kadar optimum L-Ascorbyl-2-Phosphate Magnesium dalam pakan ikan patin, Pangasius hypophthalmus ukuran sejari. Lima macam pakan isoprotein dan isokalori mempunyai kandungan L-Ascorbyl-2-Phosphate Magnesium berbeda, berturut-turut 0, 25, 50, 75 dan 100 mg/kg pakan telah digunakan dalam penelitian ini. Benih berukuran rata-rata 5,00±0,11 g dipelihara dalam akuarium dengan kepadatan 15 ekor per akuarium. Ikan diberi pakan tiga kali sehari, secara at satiation, selama 40 hari. Hasil penelitian menunjukkan bahwa kadar vitamin C tubuh meningkat sejalan dengan kadar vitamin C pakan. Demikian pula retensi protein, retensi lemak laju pertumbuhan harian, efisiensi pakan mengikuti pola yang sama seperti kandungan vitamin C tubuh (p

Effect of Enriched Feed by Different n-6 Fatty Acids Levels at 0% of n-3 on Danio rerio Reproductive Performance Utomo, N.B.P; Rosmawati, A.; Mokoginta, I.
Jurnal Akuakultur Indonesia Vol. 5 No. 1 (2006): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Lipid and fatty acids are two factors determining reproductive performance of fish and survival rate of hatched larvae. Lipid has important role as energy source and to maintain the stability of membrane permeability. The requirement of fatty acids differs among fish species. Freshwater fishes need more n-6 than n-3 fatty acids, or appropriate combination of them. In several fish species, feeding HUFA increase their fecundity, fertilization rate and eggs quality. In this study, zebra fish, Danio rerio were fed with diet containing 0% n-3 essential fatty acids and different n-6 level; 0, 1 and 2%. The result showed that there were no significant differences in gonado somatic index and several reproductive performances observed. However, 1% of n-6 fatty acids supplementation produced a higher protein and lipid content in the body by 16.85% and 55.10%, respectively. Keywords: fatty acid, n-3, n-6, Danio rerio, reproductive performance ABSTRAK Lemak dan asam lemak merupakan faktor yang sangat mempengaruhi performa reproduksi dan kelangsungan hidup larva yang menetas. Lemak berperan penting sebagai sumber energi dan menjaga kestabilan permeabilitas membran. Kebutuhan asam lemak berbeda untuk setiap jenis ikan. Ikan air tawar biasanya lebih banyak membutuhkan asam lemak n-6 daripada asam lemak n-3 atau campuran asam lemak n-6 dan n-3. Pada beberapa ikan , pemberian HUFA melalui pakan induk dapat meningkatkan fekunditas, derajat pembuahan dan kualitas telur. Pada penelitian ini ikan zebra diberi pakan mengandung asam lemak esensial dengan kadar n-3 tetap (0%) dan n-6 berbeda yaitu 0, 1 dan 2%. Hasil penelitian menunjukkan bahwa tidak ada perbedaan nyata terhadap nilai Gonado Somatik Indeks serta beberapa parameter penampilan reproduksi lainnya pada ikan zebra, Danio rerio. Namun kadar asam lemak n-3 sebesar 0% dan n-6 sebesar 1% menghasilkan kadar protein dan kadar lemak tubuh tertinggi yang masing-masing mencapai 16,85% dan 55,10% Kata kunci: asam lemak, n-3, n-6, Danio rerio, performa reproduksi

Requirement of n-6 and n-3 Fatty Acid in Broodstock Diets on Reproductive Performance of Green catfish, Hemibagrus nemurus Blkr. Utiah, Adharto; Junior, M. Zairin; Mokoginta, I.; Affandi, R.; Sumantadinata, K.
Jurnal Akuakultur Indonesia Vol. 6 No. 1 (2007): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This experiment was conducted to determine the optimum dietary level of n-6 and n-3 fatty acids on reproduction performance of green catfish. Four experimental diets with different level of n-6 and n-3 fatty acid (diet A: 0.77% n-6 fatty acid, 0.07% n-3 fatty acid; diet B: 1.56%,0.10 %; diet C: 1.74%, 0.13 % and diet D: 2%, 0.28%) were used in this experiment during seven month. Diets given twice every day in the morning and evening. The 28 broodstock used in this research with density every waring seven broodstock. Samples of eggs were analyzed for fatty acid composition. The diameter of eggs, fecundity, hatching rate of the eggs, survival rate and percentage of abnormal larvae were determined. Results showed that different dietary level of n-6 and n-3 fatty acids of the broodstock significantly affect the reproductive performance especially fecundity and hatching rate of eggs. The maximum fecundity, egg diameter, and hatching rate were produced broodstock fed on 1.56% n-6 fatty acid and 0.10 % n-3 fatty acid in diet by at the level of 12.29% lipid. Keywords : n-6 and n-3 fatty acids, reproduction performance, green catfish, Hemibagrus nemurus. Abstrak Penelitian ini dilakukan pada berbagai level asam lemak n-6 dan n-3 dalam pakan untuk melihat pengaruhnya terhadap penampilan reproduksi dari ikan baung. Penelitian dilaksanakan selama 7 bulan. Empat jenis pakan digunakan dalam percobaan ini dengan perbedaan kandungan asam lemak n-6 dan n-3 (pakan A: 0,77% asam lemak n-6, 0,56% asam lemak n-3; pakan B: 1,56%, 0,78%; pakan C: 1,74%, 1,00% and pakan D: 2,03%, 1,82%). Dalam penelitian ini 28 ekor induk digunakan dan ditebarkan kedalam waring dengan kepadatan 7 ekor tiap waring. Pakan diberikan pagi dan sore hari secara at satiation. Sampel telur dan larva dianalisa komposisi asam lemak. Penampilan reproduksi yang diamati adalah diameter telur, fekunditas, derajat penetasan telur, derajat kelangsungan hidup larva dan persentase larva abnormal. Hasil penilitian menunjukan bahwa perbedaan kandungan asam lemak n-6 dan n-3 dalam pakan berpengaruh pada komposisi asam lemak n-6 dan n-3 telur dan juga memberikan pengaruh yang berbeda nyata terhadap penampilan reproduksinya khusus pada fekunditas dan derajat penetasan telur. Lama waktu matang yang dicapai oleh tiap induk relatif sama antar perlakuan. Fekunditas, diameter telur, derajat penetasan telur dan derajat kelangsungan hidup larva tertinggi diperoleh pada induk yang memperoleh pakan yang mengandung 1,56% asam lemak n-6 dan 0,78% asam lemak n-3. Kata kunci: Asam lemak n-6 and n-3, penampilan reproduksi, ikan baung, Hemibagrus nemurus.

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