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UJI IRITASI SERTA EVALUASI SIFAT FISIK EMULGEL MINYAK ATSIRI BUNGA CENGKEH DENGAN METODE SIMPLEX LATTICE DESIGN Muhammad Fariez Kurniawan; Nining Sugihartini; Tedjo Yuwono
Farmasains : Jurnal Ilmiah Ilmu Kefarmasian Vol. 5 No. 1 (2018)
Publisher : Universitas Muhammadiyah Prof. DR. HAMKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/farmasains.v5i1.1111

Abstract

Salah satu bahan alam yang sudah terbukti mampu mengatasi inflamasi adalah minyak atsiri bunga cengkeh (MABC). Penelitian ini bertujuan untuk mengevaluasi sifat fisik dan uji iritasi sediaan emulgel MABC dengan penambahan enhancer berupa kombinasi asam oleat dan propilen glikol untuk meningkatkan kemampuan penetrasi MABC 10% MABC dengan ditambahkan enhancer 10% kombinasi propilen glikol dan asam oleat dengam metode Simplex Lattice Design (SLD) untuk mengetahui formula yang akan memiliki parameter uji fisik dan uji iritasi terbaik. Parameter uji fisik yang dilakukan adalah uji viskositas, pH, daya sebar, dan daya lekat emulgel sedangkan uji iritasi dilakukan dengan hewan uji kelinci putih jantan. Data yang diperoleh dihitung dengan metode Simplex Lattice Design. Emulgel MABC dengan penambahan enhancer kombinasi propilen glikol dan asam oleat yang dihasilkan mempunyai sifat fisik yang baik dan tidak mengiritasi kulit hewan uji. Komposisi optimum campuran enhancer sesuai metode SLD adalah 100% propilen glikol.
Optimization of Antibacterial Efficacy of Eucalyptus and Lemon Essential Oils in Hand Sanitizer with Simplex Lattice Design Muhammad Fariez Kurniawan; Revitha Nova Salsabila
PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Pharmacy, Vol. 22 No. 01 Juli 2025
Publisher : Pharmacy Faculty, Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pharmacy.v22i1.21339

Abstract

Hand sanitizer spray is a spray kind of hand sanitizer that contains active antibacterial chemicals to eliminate germs on hands. Eucalyptus globulus essential oil contains antimicrobial properties, specifically Eucalyptol (50-65%). Citrus limon essential oil exhibits antibacterial properties due to its primary component, limonene (59.7%), which damages bacterial cell walls and inhibits bacterial enzyme activity. This study aimed to assess the impact of a hand sanitizer spray containing a combination of Eucalyptus globulus essential oil and Citrus limon essential oil on the physical properties of the product, evaluate its antibacterial activity against Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli, and identify the most effective formula using the Simplex Lattice Design method. The study employed the Simplex Lattice Design approach to compare the effects of Eucalyptus globulus essential oil and Citrus limon essential oil in hand sanitizer spray formulations with ratios of 100% : 0%, 0%: 100%, and 50%: 50%. Every formula underwent physical pH, homogeneity, and viscosity tests. Antibacterial testing was conducted on Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli microorganisms—a method for testing antibacterial activity by paper disc diffusion. The ideal formula was determined by Simplex Lattice Design calculations, resulting in a blend of 70% Eucalyptus globulus essential oil and 30% Citrus limon essential oil. The hand sanitizer spray preparations were tested for antibacterial properties on Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli bacteria. The average inhibition zones were 17.33 ± 0.67 mm, 13.33 ± 0.57 mm, and 21.2 ± 4.75 mm, respectively. The preparation exhibits pseudoplastic flow behavior and has a pH of 5.5.