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All Journal Indonesian Journal of Cancer Chemoprevention Journal of Mathematical and Fundamental Sciences Indonesian Journal of Biotechnology Majalah Farmaseutik Jurnal Ilmu Kefarmasian Indonesia
Ratna Asmah Susidarti
Cancer Chemoprevention Research Center, Faculty Of Pharmacy, Universitas Gadjah Mada
Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health

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Chemical Constituents of Indonesian Micromelum minutum Leaves and Their Cytotoxicity Against MCF-7 and 4T1 Breast Cancer Cells Ratna Asmah Susidarti; Edy Meiyanto; Muthi' Ikawati; Normaidah; Nurramadhani Armada Sida
Journal of Mathematical and Fundamental Sciences Vol. 53 No. 1 (2021)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2021.53.1.7

Abstract

Micromelum minutum is used widely in traditional folk medicine. Although this species has been investigated extensively and several bioactive compounds have been isolated, little work has been done on Indonesian M. minutum. This research aimed to study the chemical constituents and biological activities of M. minutum cultivated in Bantimurung Bulusaraung National Park, South Sulawesi, Indonesia. The isolated compounds were assessed for their cytotoxicity towards MCF-7 and 4T1 cell lines by MTT method. The dried ground leaves of M. minutum were sequentially macerated with n-hexane, ethyl acetate, and methanol. The n-hexane and ethyl acetate extracts contained a flavonoid 5,7-dihydroxy-3,4',8-trimethoxyflavone (1) which inhibited MCF-7 and 4T1 cell viability by 50% at concentrations of 369±8 and 227±5 µM, respectively. Further separation of the ethyl acetate extract by column chromatography yielded acetyldihydromicromelin A (2) and a mixture of dihydromicromelin A (3) and dihydromicromelin B (4), which were not active toward MCF-7 and 4T1 cells.
Anti-metastatic effect of curcumin analog pentagamaboronon-0-fructose (PGB-0-F) against 4T1 breast cancer cells Yogi Ertanto; Rohmad Yudi Utomo; Riris Istighfari Jenie; Ratna Asmah Susidarti; Edy Meiyanto
Indonesian Journal of Biotechnology Vol 23, No 2 (2018)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8.602 KB) | DOI: 10.22146/ijbiotech.36431

Abstract

Development of a chemotherapeutic agent and boron carrying pharmaceutical based on triple-negative breast cancer is important due to its metastatic progression. Metastases are often more dangerous than the primary tumor and they are responsible for 90% of all cancer deaths. The purpose of this study was to explore the anti-metastatic activities of the PGB-0 complex with fructose (PGB-0-F) against 4T1 breast cancer cells. A scratch wound healing assay was carried out to determine the migration inhibition ability of PGB-0-F, while MMP-9 expression was analysed using gelatin zymography. The testing of anti-migration activity showed that PGB-0-F inhibited in 4T1 cells, whereas the gelatin zymography assay revealed a suppression of MMP-9 expression. PGB-0-F inhibited closure on 4T1 metastatic breast cancer cells line compared with the control. PGB-0-F decreased the MMP-9 expression level compared with the control. Based on these results, PGB-0-F has the potential to be developed as a chemotherapeutic agent, and especially as an anti-metastatic agent.
Pemodelan Molekular Enzim 3β-Hydroxysteroid Dehydrogenase Tipe 2: Pemodelan Kombinasi Homologi, Docking dan Pendekatan QSAR BAMBANG SULISTYO ARI SUDARMANTO; AGUSTINUS YUSWANTO; RATNA ASMAH SUSIDARTI; SRI NOEGROHATI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 15 No 1 (2017): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

A homology model of human 3β-HSD type 2 has been developed from homology modeling techniques using Phyre2 server and refi ned by ModRefi ner. The PROCHECK, QMEAN and ProSA-web online tools were carried out to evaluate the stereochemical quality of the model. The Ramachandran plot resulted from PROCHECK showed that 84.5% residues are in the most favored region, 13.7% are in the additional allowed region, 1.5% are in the generously allowed region and 0.3% are in the disallowed region. The QMEAN (Z-score) are 0.509 (-3.006) and Z-score of ProSA-web is -7.10. The negative values of protein fold energies also found in almost all sequences. Furthermore, molecular docking was also applied to validate the model using MOE. The hydrogen bonding interactions with Tyr154, Ser124, and Ser218 are found in all docked substrates as well as known inhibitors (trilostane and epostane). A dataset of azasteroid inhibitors were also docked into the substrate active site of human 3β-HSD2. These docked structures were utilized to construct corresponding docking-based QSAR equation by employing genetic algorithm (GA) statistical analysis. The contructed best QSAR equation has a robust predictive power according to its statistical parameters, hence may be applied to supersede the default scoring function provided by docking software. These results indicate that the human 3β-HSD2 model was successfully evaluated as a good model.
Peningkatan Efek Sitotoksik Doxorubicin oleh Naringenin pada Sel Kanker Payudara T47D melalui Induksi Apoptosis SENDY JUNEDI; RATNA ASMAH SUSIDARTI; EDY MEIYANTO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 8 No 2 (2010): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Doxorubicin is one of the standard regiment for breast cancer chemotherapy, but resistance to this agent is often occured and long period usage will induce cardiotoxicity, Therefore, combination therapy (co-chemotherapy) is needed to improve the efficacy of doxorubicin and to reduce its systemic toxicity. Naringenin is one of the most abundant ilavonoids in citrus fruits which showed cytotoxicity in various human cancer cell lines and has mechanisms through pathways except p53. This research is aimed to examine the effect of naringenin in combination with doxorubicin against T47D breast cancer cell which is resistant to doxorubicin due to p53 mutation. The IC50 dan CI (combination index) values were detennined by the MTT assay, The apoptotic stimulation effect of narigenin and doxorubicin was performed by DNA staining using cthidum bromide-acridine orange. Naringenin and doxorubicin exhibited cytotoxic effect with IC50 of 509 µM and 15 nM, respectively. The CI value in all ratios of naringenin-doxorubicin showed synergistic effect (CI 0.20-0.89). Combination ofnaringenin-doxorubicin with concentration smaller than 12,5 µM-0.6 nM in 1:1 ratio exhibited an additive combination effect, The combined treatment increased the apoptotic effect of doxorubicin.These results show that naringenin is potential to be developed as co-chemotherapeutic agent with doxonibicin, although the molecular mechanism is still needed to be explored.
Synergistic Effect of Areca catechu L. Ethanolic Extract and Its Chloroform Fraction with Doxorubicin on MCF7 EDY MEIYANTO; SRI HANDAYANI; ENDAH PUJI SEPTISETYANI; RATNA ASMAH SUSIDARTI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 7 No 1 (2009): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Ekstrak etanol biji buah pinang (Areca catechu L.) menunjukkan efek sitotoksik pada sel kanker MCF7 dan WiDr. Penelitian ini bertujuan untuk mempelajari efek sinergisme antara ekstrak etanol biji buah pinang (AE) dan fraksi kioroforrnnya (ACF) dengan doxorubicin (Dox) dalam pemacuan apoptosis sel MCF7. Ekstrak etanol dipartisi dengan n-heksan dan kloroform untuk mendapatkan fraksi kloroforin. Efek sitotoksik.AE, ACF, dan Dox pada perlakuan tunggal dan kornbinasi ditentukan dengan metode MTT. Penganiatan apoptosis dilakukan dengan pengecatan DNA dengan akridin oranye-etidium bromida (double staining). Imunositokimia dilakukan untuk melihat ekspresi COX-2 dan Bax. Kombinasi Dox 100, 250, 334, dan 500 nM dengan AE 8 µg/ml; Dox 100 nM dengan AE 20 µg/ml; serta Dox 100 dan 250 nM dengan ACF 7 dan 18 µg/ml memperlihatkan efek sinergistis yang kuat (CI 0,1-0,3). Sernentara itu, kombinasi Dox 250, 334, dan 500 nM dengan AE 20, 27, dan 40 µg/ml; Dox 100 nM dengan AE 27 dan 40 µg/ml; Dox 100 nM dengan AE 20 µg/mi; Serta 500 nM dengan ACF 24 dan 35 µg/ml menunjukkan efek sinergistis (CI 0,3-0,7). Secara keseluruhan, kombinasi AE dan ACF dengan Dox memperiihatkan efek sinergistis pada MCF7 dengan indeks kombinasi (CI) kurang dari 0,9 (P<0,05). Periakuan kombinasi juga memacu apoptosis. Penekanan ekspresi Bcl-2 terjadi padaperlakuan kombinasi ACF-Dox. Hasil penelitian ini menunjulckan bahwa koinbinasi AE dan ACF dengan Dox memberikan efek sinergistis dalam pemacuan apoptosis dengan kemungkinan mekanisme meialui penekanan ekspresi Bcl-2.
Ekstrak Air J amur Ling Zhi (Ganoderma lucidum (Leysser) Karsten) Meningkatkan Persentase Sel Limfosit T CD8+ Relatif pada Tikus yang Dipejani Doxorubicin MUTHI IKAWATI; ANNISA KARAMITA; EDIATI EDIATI; RATNA ASMAH SUSIDARTI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 9 No 1 (2011): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1963.698 KB)

Abstract

Cancer therapy using chemotherapeutic agents associated with many adversed side effects, including immunosupressant. The use of immunostimulator together with chemotherapeutic agent (co-chemotherapy) can be the alternative method to solve that problem. Ganoderma lucidum (Ling Zhi) has been reported to have immunostimulatory activity. The aim of this research was to evaluate the immunostimulatory activity of water extract of G. Zucidum by determining the relative CD8+ T lymphocyte cell percentage in rats induced by doxorubicin. Extraction of plant material was carried out by infusion method. Sprague Dawley female rats were divided into six groups, they were doxorubicin as control, commercial product as comparing control, 100 mg/kgBW and 450 mg/kgBW extract treatment, extract control, and without treatment control. Relative CD8+ T lymphocyte cell percentages of blood samples were obtained by flow cytometry by using Multiset program. The data were analyzed statistically using paired sample t test and one way ANOVA continued by Post Hoc test. The result showed that the water extract of G. lucidum increased the relative CD8+ T lymphocyte cell percentage in rats induced by doxorubicin. The water extract of G. lucidum is promising to be developed as co-chemotherapy immunostimulatory agent.
Pemacuan Apoptosis oleh Fraksi Kloroform Kulit Batang Bruguiera gymnorhiza pada Sel Kanker Rahim HeLa WARSINAH WARSINAH; SISMINDARI SISMINDARI; RATNA ASMAH SUSIDARTI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 10 No 2 (2012): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

PENGARUH KONSENTRASI MAHKOTA DEWA TERHADAP STABILITAS LOTION – KRIM SERTA UJI TABIR SURYA SECARA SPEKTROFOTOMETRI Abdul Karim Zulkarnain; Marchaban Marchaban; Subagus Wahyuono; Ratna Asmah Susidarti
Majalah Farmaseutik Vol 11, No 3 (2015)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (972.362 KB) | DOI: 10.22146/farmaseutik.v11i3.24124

Abstract

Ekstrak daun mahkota dewa mengandung senyawa turunan benzofenon yang memiliki aktivitas sebagai tabir surya. Penelitian ini bertujuan untuk mengetahui stabilitas fisik dan kimia lotion dan krim o/w serta aktivitasnya sebagai tabir surya dengan spektrofotometer. Ekstrak diperoleh dengan metode maserasi metanol lalu diformulasi menjadi Lotion dan krim o/w serta diuji stabilitas fisik dan kimianya serta diuji SPF nya secara in vitro dengan spektrofotometer. Hasil studi menunjukkan bahwa formula lotion dan krim o/w ekstrak mahkota dewa stabil selama penyimpanan 6 minggu. Kenaikan konsentrasi mahkota dewa akan menaikkan viskositas Lotion dan krim o/w secara signifikan. Krim selama penyimpanan lebih stabil homogenitasnya dibanding dengan lotion yaitu pada minggu ke enam minyak dari sediaan lotion mulai terlihat warna coklat dipermukaannya sedangkan krim lebih viskes dibanding dengan lotion. Sediaan selama penyimpanan 6 minggu memiliki kandungan phalerin yang relatif stabil. Aktivitas sediaan secara in vitro menunjukkan bahwa nilai SPF pada kadar ekstrak mahkota dewa 6 %, 8 % dan 10 % berturut turut untuk krim adalah 8,60, 11,51, 16,04 sedangkan SPF untuk lotion adalah 7,45, 10,83 dan 15,01 %. Sediaan lotion dan krim mahkota dewa stabil selama penyimpanan dan memiliki aktivitas sebagai tabir surya.
Co-Authors Abdul Karim Zulkarnain Adam Hermawan AGUSTINUS YUSWANTO ANNISA KARAMITA Aulia Katarina BAMBANG SULISTYO ARI SUDARMANTO EDIATI EDIATI Edy Meiyanto EDY MEIYANTO Edy Meiyanto Edy Meiyanto ENDAH PUJI SEPTISETYANI Fany Mutia Cahyani Herwandhani Putri Indri Kusharyanti Inna Armandari Jenie, Riris Istighfari Kartika Dyah Palupi Kholid Alfan Nur Larasati Larasati Layung Sekar Sih Wikanthi Marchaban Marchaban Marvin Lambertus Muthi Ikawati Nindi Wulandari Normaidah, Normaidah Nur Fitra Sari Nurramadhani A. Sida Rahmi Khamsita Rahmi Khamsita Raisatun Nisa Sugiyanto Raisatun Nisa Sugiyanto Riris Istighfari Jenie Rohmad Yudi Utomo SENDY JUNEDI SISMINDARI SISMINDARI Sofa Farida Sri Handayani SRI HANDAYANI Sri Handayani Sri Noegrohati Subagus Wahyuono WARSINAH WARSINAH Yogi Ertanto Yuni Fajar Esti Zalinar Udin