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Toxicity test of mangosteen peel extract (Garcinia mangostana L.) as denture cleanser of heat-cured acrylic resin: in vitro experimental laboratory Tertya, Bertha Bening; Kristiana, Dewi; Naini, Amiyatun
Padjadjaran Journal of Dentistry Vol 36, No 1 (2024): March 2024
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol36no1.53363

Abstract

ABSTRACTIntroduction: Mangosteen peel extract can be used as a denture cleaning agent to effectively inhibit the growth of C. albicans because mangosteen peel contains various compounds such as mangostin, tannins, flavonoids, saponins. Denture cleaning materials that will be used in the dental field must meet biocompatibility requirements, one of which is a toxicity test. The aim of this study was to analyze the toxicity of mangosteen peel extract as a cleaner of heat cured acrylic resin denture on BHK-21 as cell culture of fibroblast cells. Methods: Toxicity test used the MTT assay method. Acrylic resin samples had a diameter of 3 mm and a thickness of 2 mm. The acrylic resin was soaked for 4 days, equivalent to soaking 15 minutes per day for 1 year in mangosteen peel extract with concentrations of 50, 60, 70%, and sodium hypochlorite. Analyzed data using normality and homogeneity test results showed (p<0.05). Kruskal Wallis test results (0.000<0.05) and Mann Whitney (0.000<0.05). Results: The greatest cell viability value was shown in mangosteen peel extract with a concentration of 50%, namely 97.16939%. Based on research data, it can be said that the lower the concentration of mangosteen peel extract, the lower the level of toxicity of a denture cleanser agent. Mangosteen peel extract in concentrations of 60, 70%, and sodium hypochlorite are toxic when used as a denture cleanser. Conclusion: Mangosteen peel extract with a concentration of 50% is not toxic to BHK-21 fibroblast cells as cell culture of and as a cleaner of heat cured acrylic resin denture on.Keywordheat-cured acrylic resin, mangosteen peel extract, MTT assay.
KARAKTERISASI SCAFFOLD HYDROXIAPATITE GYPSUM PUGER KOMBINASI ALGINAT RUMPUT LAUT COKLAT (SARGASSUM SP) SEBAGAI BONE GRAFT DENGAN METODE FREEZE DRYING Safira Annisa Yasmin Pambudi; Amiyatun Naini; Agus Sumono
Cakradonya Dental Journal Vol 14, No 2 (2022): Agustus 2022
Publisher : FKG Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/cdj.v14i2.29947

Abstract

Tujuan dari penelitian ini adalah menganalisis karakterisasi bahan Scaffold HAGP dengan kombinasi alginat rumput laut coklat (sargassum sp) menggunakan FTIR (Fourier Transform Infra Red. Menganalisis bentuk porositas dari bahan Scaffold HAGP dengan kombinasi alginat rumput laut coklat (sargassum sp) dengan SEM (Scanning Electron Microscopy) dan melihat prosentase porositas dari bahan Scaffold HAGP dengan kombinasi alginat rumput laut coklat (sargassum sp) dengan menggunakan uji porositas. Metode penelitian menggunakan metode deskriptif. Metode analisis data menggunakan FITR, dan uji porositas. Hasil penelitian menunjukkan bahwa karakterisasi bahan Scaffold HAGP dengan kombinasi alginat rumput laut coklat (sargassum sp) menggunakan FTIR (Fourier Transform Infra Red) dijelaskan bahwa pola FTIR hidroksiapatit gipsum Puger (HAGP) dan gabungan pola FTIR HAGP dengan Alginat nampak ada kemiripan pola FTIR HAGP. Bentuk porositas dari bahan Scaffold HAGP dengan kombinasi alginat rumput laut coklat (sargassum sp).
Pelatihan dan Pendampingan Kader Kesehatan Lansia di wilayah Kerja Puskesmas Gladakpakem untuk Meningkatkan Kesehatan Gigi dan Mulut Dewi Kristiana; Amiyatun Naini; Ady Soesetijo; Rahardyan Parnaadji; Achmad Gunadi; Afif Surya Adena; Ardhianing Hardita
Dental Agromedis Vol. 2 No. 2 (2024): November
Publisher : Fakultas Kedokteran Gigi, Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/da.v2i2.1719

Abstract

In order to achieve dental and oral health indicators, promotive and preventive efforts are needed in addition to curative and rehabilitative measures. Promotive action is by conducting training for elderly cadres about dental and oral health for the elderly and counseling about dental and oral health for the elderly. The dental and oral health status of the elderly in the working area of the Gladapakem Health Center has received little attention. As well as the knowledge and attitude of the elderly towards dental and oral health is also low; it can be seen that many older adults with no teeth are left without wearing dentures. Elderly cadres at Posyandu have a significant role. Based on interviews with nurses holding the elderly Posyandu program in the working area of the Gladakpakem Health Center, Sumbersari District, Jember Regency, the activities carried out so far are checking blood pressure, body weight, if there are complaints referred to the Health Center. Meanwhile, dental and oral examination activities and counseling on dental and oral health carried out by health cadres are rarely done. Knowledge of dental and oral health cadres also needs to be improved. So the servant carried out the service by holding training and mentoring for elderly health cadres in Posyandu Catleya Kebonsari Village and Kranjingan Village regarding dental and oral health. From the results of community service activities, it can be concluded that there is an increase in skill knowledge about elderly dental and oral health in elderly cadres.Keywords: dental and oral health, elderly patient, posyandu.
The effect of cocoa (Theobroma cacao L) on the basic color stability of thermoplastic nylon resin dentures Naini, Amiyatun
Padjadjaran Journal of Dentistry Vol 23, No 3 (2011): November 2011
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1567.414 KB) | DOI: 10.24198/pjd.vol23no3.14039

Abstract

Nylon thermoplastic resin is material of choice for the making of flexible. This denture do not use wire retention, but has the physical properties of water absorption. In the oral cavity, it will always be in contact with food and beverages consumed. One of the foods that are consumed by the public is chocolate. This study aimed to determine the effect of cocoa (Theobroma cacao L) on color stability of the thermoplastic nylon denture base. The study sample was thermoplastic nylon (valplast) with a size of 10x10x2 mm soaked in the chocolate solution for 7 and 14 days. As the control, the sample soaked with distilled water. The color testing stability used was densitometer. There were significant differences between the control group (distilled water) and the chocolate solution. This was due to dissolved components/tannin having a capillary flow diffusion into thermoplastic nylons that causing discoloration. The conclusion of this study, there was the effect of cocoa (Theobroma cacao L) against the color stability of the nylon thermoplastic denture base. The longer time of immersion of nylon thermoplastic the greater the change in color.
Effect of 25% Tobacco Leaf Extract (Nicotiana Tabacum L.) as a Denture Cleaning Paste on Surface Roughness and Color Change Thermoplastic Nylon Parnaadji, Rahardyan; Naini, Amiyatun; Soesetijo, FX Ady; Kristiana, Dewi
Jurnal Riset Kesehatan Vol 13 No 1 (2024): MEI 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jrk.v13i1.10677

Abstract

Thermoplastic nylon as a denture base requires mechanical cleaning with denture paste. Previous research showed that tobacco leaf extract paste (TLEP) 25% was fungistatic on thermoplastic nylon denture bases. The aim is to evaluate the roughness and colour changes of thermoplastic nylon against tobacco leaf extract paste 25% during one year of simulated use. This research hypothesizes that thermoplastic nylon's roughness and colour value will decrease further after brushing using 25% TLEP. Sample groups include brushing without paste, paste without TLEP 25% and TLEP 25%. The sample size for each group was 15 samples; measurements use a surface roughness tester and a colour reader. Brushing was carried out for 23.5 minutes on a plate measuring 60x10x2.5 mm as a surface roughness test and 3.90 minutes on a plate measuring 10x10x2.5 mm as a colour change test. The sample size for each group was 15 samples; measurements use a surface roughness tester and a colour reader. The research results were tested using Kolmogorov-Smirnov and Levene and One-way ANOVA with a significance level of 0.05. There were no differences in surface roughness and colour changes on thermoplastic nylon plates after brushing with 25% TLEP (p>0.05). This study concluded that 25% TLEP did not affect increasing surface roughness and changing the colour of thermoplastic nylon plates with values below the threshold.
Socket Preservation with Hydroxyapatite Gipsum Puger Scaffold and Aloe vera on Fibroblast and Type 1 Collagen Cells Amiyatun Naini; Clarissa Astiasari; Muhammad Nurul Amin
Denta Journal Kedokteran Gigi Vol 16 No 1 (2022): February
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v16i1.6

Abstract

Background: The prevalence of tooth extraction was 7.29% according to RISKESDAS 2018. Post-tooth extraction will cause injury and bone resorption. Scaffold material combination of hydroxyapatite gypsum puger (HAGP) and aloe vera (AV) can be used for preservation of extraction socket. Objective: To analyze fibroblast and type 1 collagen cells in the extraction socket after being induction by a combination of hydroxyapatite gypsum puger scaffold and aloe vera. Materials and Methods: Making a combination of gypsum puger hydroxyapatite scaffold and aloe vera, divided into four groups, namely negative control, aloe vera scaffold, HAGP scaffold, and HAGP+AV scaffold. Extraction of the mandibular 1st molar in Wistar rats, the application on the extraction socket was waited for 3, 5, 7, and 14 days. Preparation of preparations using Hemaktocillin Eosin staining method. Results: LSD test on the HAGP+AV scaffold group between days 3 and 5, days 3 and 7, days 3 and 14, days 5 and 7, days 5 and 14, days 7, and 14 were obtained (p=0.000), this shows a significant difference between the treatment groups. The results of type 1 collagen in the HAGP+AV scaffold group between days 3 and 14 were obtained (p=0.005) showing a significant difference. Each group on day 14 showed the highest number of fibroblast and type 1 collagen cells. Conclusion: The combination of HAGP+AV+gelatin scaffold can increase fibroblast cells and type 1 collagen after tooth extraction. The socket healing process is getting faster on day 14.
Role of cassava starch (Manihot esculenta Crantz) in reducing candida albicans adhesion on soft denture liners: an experimental study Adena, Afif Surya; Naini, Amiyatun; Soesetijo, FX Ady; Kristiana, Dewi; Parnaadji, Rahardyan; Hardita, Ardhianing
Padjadjaran Journal of Dentistry Vol 38, No 1 (2026): March 2026
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol38no1.68181

Abstract

Introduction: Soft denture liners can be an alternative solution for various removable denture problems, but they still have several limitations, such as promoting Candida albicans adhesion to dentures. Modifications incorporate cassava starch. The aim of this result is toanalyze the role of cassava starch in reducing Candida albicans adhesion on soft denture liners. Methods: This laboratory experimental study used twenty-seven disc-shaped heat-cured acrylic plates with a diameter of 10 mm and a thickness of 2 mm divided into three groups: acrylic plate without a coating (negative control group), acrylic plate coated with soft denture liners (positive control group), and acrylic plate coated with a soft denture liner containing cassava starch 15% (treatment group). The samples were soaked in artificial saliva for 1 hour, then contaminated with Candida albicans by placing them in a test tube containing a Candida albicans suspension, and incubated for 24 hours at 37°C. Samples were rinsed twice with PBS, then placed in a test tube containing 10 mL of Sabouraud's broth and vortexed for 30 seconds. The turbidity of the released Candida albicans was measured using a spectrophotometer. Absorbance data were analyzed using one-way ANOVA and post hoc LSD. Results: Turbidity testing using a spectrophotometer at 600 nm showed the average absorbance values were 0.013 for negative control group, 0.028 for the positive control group, and 0.019 treatment group. The results of the one-way ANOVA test showed a p-value of 0.004 (<0.05), indicating a significant difference between the groups. Further post hoc LSD tests showed that the positive control group had the highest absorbance value. At the same time, there was no significant difference between the negative control group and treatment group. Conclusion: Cassava starch inhibits Candida albicans adhesion to the soft denture liners.