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Journal : Journal of Fisheries

Pengaruh Pemberian Hormon Igf-I (Insulin-Like Growth Factor-I) Recombinant Mouse terhadap Pertumbuhan Ikan Gurame (Osphronemus Gouramy) [ Effect of hormones Recombinant Mouse IGF-I (Insulin-Like Growthfactor-I) on The Growth of Osphronemus gouramy] Woro Hastuti Satyantini; Prayogo Prayogo; Dimas Eraparamarta Harkitiyanto
Jurnal Ilmiah Perikanan dan Kelautan Vol. 8 No. 1 (2016): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v8i1.11190

Abstract

Abstrak Konsumsi ikan gurame semakin meningkat setiap tahun mengakibatkan tingginya jumlah permintaan, belum dapat diimbangi produksi yang mencukupi. Laju pertumbuhan ikan gurame dikenal lambat, sehingga untuk mencapai ukuran konsumsi diperlukan waktu pemeliharaan relatif lama. Peningkatan kualitas produksi dengan meningkatkan kemampuan metabolisme sel terhadap asupan pakan ikan melalui penyuntikan hormon insulin-like growth factor-I(IGF-I) diharapkan dapat memacu pertumbuhan, dan produksi ikan dapat ditingkatkan. Tujuan dari penelitian ini adalahuntuk mengetahui pengaruh pemberian hormon (IGF-I) recombinant mouse terhadap peningkatan pertumbuhan ikan gurame(Osphronemus gouramy). Penelitian adalah metode eksperimental rancangan acak lengkap (RAL), menggunakan ikan gurame sebanyak 80 ekor yang terbagi dalam 4 perlakuanP0 : tanpa disuntik hormon IGF-I, P1 : disuntik hormon IGF-I dengan dosis 10 ng/ml, P2 : disuntik hormon dengan dosis 20 ng/ml dan P3 : disuntik hormon dengan dosis 40 ng/ml masing-masing 5 ulangan setiap ulangan 4 ekor ikan gurame. Perlakuan dengan injeksi hormon IGF-I recombinant mouse. Parameter yang diukur adalah pertumbuhan. Hasil penelitian menunjukkan bahwa perlakuan penyuntikan hormon IGF-I recombinant mouse dengan dosis yang berbeda memberikan perbedaan yang nyata (p<0,05); pertumbuhan berat (P0: 0 ng/ml = 7,23 gram± 0,37 gram dan P3: 40 ng/ml = 26,35 gram± 1,89 gram) ;pertumbuhan panjang (P0 : 0 ng/ml = 5,76 cm ± 0,38 cm dan P3 : 40 ng/ml = 7,93 cm± 0,07 cm).). Kesimpulan penelitian bahwa IGF-I recombinant mouse berpengaruh meningkatkan pertumbuhan pada peningkatan berat dan panjang ikan gurame pada perlakuan penyuntikan 40 ng/ml.  Abstract Osphronemus gouramy consumption is increasing every year which causes in a high number of inquiries for this fish, cannot be offset sufficiently by the amount of production. The growth rate of O. gouramy are known relatively slow, so that to achieve the required consumption is relatively long. Quality improvement of productivity by improving the ability of cell metabolism to intake of fish feed through hormone injection recombinant mouse insulin-like growth factor-I (IGF-I) is expected to spur growth, so that fish production can be increased. The aim of this study is to determine the effect of the hormone IGF-I recombinant mouse to the increased growth of O.gouramy. The benefits of this research are expected to deliver the benefitsof science in the form of scientific information to the public in general and fish farmers especially about the effect of the hormone recombinant mouse IGF-I on the growth of O. gouramy.The research is methodis carried out experimentally by using completely randomized design (CRD),this study uses 80 O. gouramy were divided into 4 treatment P0: without hormone injectionsof IGF-I, P1: injected with the hormone IGF-I at a dose of 10 ng/ml, P2: injected with the hormone IGF-I at a dose of 20 ng/ml and P3: injected with the hormone ata dose of 40ng/ml each of 5 replicates each test 4 O. gouramy. The hormone IGF-I recombinant mouse is done by injection. The treatment with hormone injection of recombinant mouse IGF-I. Parameters measured were growth. The results showed that the treatment hormone injection of recombinant mouse IGF-I with different doses gave significant differences (p <0.05);weight growth (P0: 0 ng/ml = 7,23 gram ± 0,37 gram dan P3: 40 ng/ml = 26,35 gram ± 1,89 gram.); length growth (P0 : 0 ng/ml = 5,76 cm ± 0,38 cm dan P3 : 40 ng/ml = 7,93 cm ± 0,07 cm). The conclusion that recombinant mouse IGF-Iincreased the growth by increasing in weight and length of O. gouramy and improvement of the growth occurred in the treatment injection of the dose: 40ng/ml. 
Substitusi Silase Secara Kimiawi Limbah Padat Surimi Ikan Swanggi (Priacanthus macracanthus) pada Tepung Ikan terhadap Retensi Energi dan Rasio Konversi Pakan Benih Ikan Nila (Oreochromis niloticus) [Chemical Silage Substitution of Swanggi Surimi Solid Waste (Priacanthus macracanthus) of Fish Meal Toward Energy Retention and Tiplapia Seeds (Oreochromis niloticus) Feed Conversion Rate] Boedi Setya Rahardja; Prayogo Prayogo; Wiku Bakti Bawono
Jurnal Ilmiah Perikanan dan Kelautan Vol. 7 No. 2 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v7i2.11203

Abstract

Abstract Swanggi surimi waste can be processed into economically valuable source of protein through chemical treatment processes produce crude protein content ranging from 51%. Results of high energy retention and reduced feed conversion ratio of solid waste as a substitute swanggi fish surimi fish meal is expected to optimize the growth of tilapia with lower feed prices, thereby reducing the high cost of feed in aquaculture. This study aims to determine the chemical silage substitution of solid waste swanggi fish surimi (P. macracanthus) in fish meal. The experimental design completely randomized design (CRD) with four treatments with five replications. The treatment used is the number of different proteins in each feed rations. The main parameters are observed energy retention (%) and feed conversion ratio. Analysis of data using analysis of variance (ANOVA) and followed Duncan's multiple range test. These results indicate that substitution is chemically silage solid waste swanggi surimi fish in fish meal provides highly significant effect (p <0.05) on the retention of energy and feed conversion ratio tilapia fish. The average retention of the highest energy on a few observations are in treatment P3 (67,90a ± 0.86%) and the lowest at P0 (64,72b ± 1.17%). Average feed conversion ratio was lowest for the treatment P3 (2,41c ± 0.028) and the highest pda P0 (2,50a ± 0.018).
Pengaruh Bioabsorpsi Mangrove Avicennia alba terhadap Limbah Amoniak (NH3) [The Bioabsorbtion Effects of Mangrove Avicennia alba against Ammonia (NH3) ] Moch. Amin Alamsjah; Mega Puspa Sari; Prayogo Prayogo
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 2 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i2.11308

Abstract

Abstract The pressure on the aquatic environment progressively increasing due to the influx of waste from various activities in areas that have been built in coastal areas. Waste is one of the issues that must be handled properly. Inadequate waste management with the application of inappropriate technology will cause negative effects on the environment because the waste contains harmful chemicals and toxic. One of the common chemicals contained in the waste is ammonia. Utilization of aquatic plants can be used to overcome the problem of ammonia. Mangrove Avicennia alba is a water plant that can be used to absorb ammonia in the water. This research is conducted to determine the effect and the ability of mangrove A. alba as bioabsorption of ammonia in the water. Based on the research conducted, A. alba can act as bioabsorbtion for ammonia in the water. A. alba can absorb ammonia content in water from 10 mg/L to 0 mg/L within seven days with the highest uptakes are in treatment of P3 (5 mg/L) and treatment of P4 (10 mg/L). Treatment of P2 (1 mg/L) is the highest average nitrogen contain with 0.9244 %.
Pengaruh Padat Tebar Ikan Lele Terhadap Laju Pertumbuhan dan Survival Rate Pada Sistem Akuaponik [ The Effect Of Stocking Density On Survival Rate and Grow Rate Of Aquaponic System] Boedi Setya Rahardja; Ongky Wijaya; Prayogo Prayogo
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 1 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i1.11382

Abstract

AbstractIncreasing consumption of catfish and dairy products encourage increased domestic production of catfish. During the period of 5 years (2005-2009), catfish production has been increased significantly with an average annual growth reached 32%. In 2008 production reached 114.371 tonnes and in 2009 production increased by almost 75% to about 200 thousand tons. Aquaponic is a bio-integration that links recirculating aquaculture principles to the production of crops / vegetables hydroponically (Diver, 2006). Aquaponic technology has proven to successfully produce an optimal fish on less land and limited water resources, including in urban areas (Ahmad, 2007). This study aims to find out the influences of catfish stocking density difference on the growth rate and survival rate of catfish on aquaponic. The experimental design used was completely randomized design (CRD). Analysis of the data processed using Analysis of Variance (ANOVA) to know whether there is influance the growth rate and survival rate between the treatment given. If there are significantly difference then proceed by Duncan's Multiple Range Test. Based on the research obtained the result that there are significant differences in the survival rate (p> 0.01) and there is a significant difference to the rate of growth (p> 0,01). From the results of statistical tests that have been done show that the highest survival rate is in treatment 4 (87.5333%) and the highest growth rate is in treatment 4 (30.5333%). The lowest Survival found in treatment 1 (53.134%). Lowest growth rate found in treatment 1 (16.6%).
Potensi Senyawa Metabolit Sekunder Infusum Daun Durian (Durio zibethinus) Terhadap Kelulushidupan Ikan Nila (Oreochromis niloticus) Pada Transportasi Ikan Hidup Sistem Kering [ The Secondary Metabolites Potential Of Infusum Durian's (Durio zibethinus) Leaves Effect To Survival Rate Of Nila (Oreochromis niloticus) On The Livefish Dry Transport System] Endang Dewi Masithah; Muhammad Sholihul Abid; Prayogo Prayogo
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 1 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i1.11386

Abstract

Abstract Nila (Oreochromis niloticus) is one of the leading commodity and each year will always be increased in both local and export markets. It's in line and will continue to grow due to the tendency of shifting market demand for commodities is of the dead form of fish (frozen) or other processed into a form of life, especially for the needs of breeders on commodity stocks. One of the latest post-harvest technologies that can be applied in the handling of live fish is a fish transport system in the dry media without use of water as a medium of transport with the use of anesthesia using plants that naturally contain active compounds that have the potential as a anesthesia drugs. This study aimed to determine the length of time different fish living on the transportation system with particular concentration infusum dried leaves of durian (Durio zibethinus) effect on the survival rate of nila (O. niloticus). Methods of this study is an experiment with three replications with different concentration intervals. It consisted of a preliminary study to determine the potential infusum durian leaves as anesthesia and primary research material as a form of media applications on the system dry. This study was conducted at the Laboratory of the Faculty of Fisheries and Marine Airlangga University. The results showed that the effective concentration of durian leaves infusum used to transport test was 4100 ppm with an average induction time is 40 minutes and recovery time is 48 minutes. Durian leaves infusum use in nila with 4100 ppm concentration able to achieve the level of survival and long transportation times in a row 100%, 0 h, 88.89%; 2 h, 22.22%; 4 h, 0%; 6 h, and 0%, 8 h on media packaging live fish without water.
Efektivitas Perasan Asam Jawa (Tamarindus indica L) Terhadap Pertumbuhan Bakteri Aeromonas hydrophila dengan Metode Difusi Kertas Cakram [Effectiveness Of Thamarin Juice (Tamarindus indica L) On Growth Of Bacteria Aeromonas hydrophila With Paper Disc Diffusion Method] Laksmi Sulmartiwi; Rizky Aprilia Chrisanti; Prayogo Prayogo
Jurnal Ilmiah Perikanan dan Kelautan Vol. 4 No. 2 (2012): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v4i2.11560

Abstract

Abstract Fish disease is one of the serious problems that faced by the farmers potentially to make high disadvantages as follows, mortality of fish and fish quality decrease, therefore economically will caused price decrease. Bacterial pathogens were one of the problems that were faced by fish farmers, which cause 90% of mortality. Diseases control caused by Aeromonas hydrophila in general using by antibiotics. Antibiotics are commonly used were tetracycline, oxolinic acid, erytromicin, streptomycin, and chloramphenicol. Using of antibiotics has shown good results, but on the other hand using of antibiotics or antibacterial cultivation would increased the bacteria number of resistant to antibiotics. This research was purposed to find the alternative treatment of Motile Aeromonas Septicemia by determination ability of a squeeze of tamarind (Tamarindus indica L) and the minimum concentration of tamarind (Tamarindus indica L) to inhibit the growth of Aeromonas hydrophila. The function of tamarind as an antibacterial because they contain antibacterial substance such as flavonoids and saponins. The results showed that the antibacterial power of tamarind juice has antibacterial power against Aeromonas hydrophila. Based on a sentsitivity test was performed according to standard of antibiotic concentrations that are common to Aeromonas hydrophila, was Tetracycline inhibition zone with <15 mm diameter (not sensitive), 15-18 mm (moderately susceptible), >18 mm (very sensitive) is A (10%), and B (12.5%), treatment inhibition zone subsequently 13 mm and 14.3 mm (insensitive), C (15%), D (17.5%), with inhibition zone 15.5 mm and 16.6 mm (self-sensitive) and 20% with inhibition zone diameter of 19 mm (highly sensitive). Therefore, it was concluded that A treatment (15%) with inhibition zone 15.5 mm showed the inhibition ability of Aeromonas hydrophila growth.
Eksplorasi Bakteri Indigen pada Pembenihan Ikan Lele Dumbo (Clarias sp.) Sistem Resirkulasi Tertutup [Exploration Of Indigen Bacteria From Catfish (Clarias sp.) Breeding On Closed Resirculation System ] Prayogo Prayogo; Boedi Setya Rahardja; Abdul Manan
Jurnal Ilmiah Perikanan dan Kelautan Vol. 4 No. 2 (2012): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v4i2.11571

Abstract

Abstract The efforts of the catfish hatchery fish are generally confined to the central areas of hatchery that has abundant water resources. Solving the problem of limited water, appears a catfish hatchery system with a closed recirculation system. In such systems the process of biological filtration is the most important thing. It shows the handling of organic materials by utilizing the degrading bacteria is the key to successful management of the system. Thus necessary to be studied the role of bacteria degrading organic matter indigen (local bacteria) in the closed hatchery recirculation system of catfish. This study aimed to obtain bacterial strains degrading bacteria indigen as organic materials are very useful in improving water quality in the closed hatchery recirculation system of catfish and knowing the methods to the use of bacteria indigen as degrading organic matter. The method used in this study was designed based on the stages of research carried out in accordance with the objectives to be achieved. The results of the isolation and identification to the species level showed Pseudomonas stutzeri and Pseudomonas pseudomallei obtain the greatest value for hydrolysis index representing each trait protease, lipase and amylase. Bioremediation effectiveness test in vitro showed effective results in the treatment using consortia of bacteria inoculant. Consortia of bacteria inoculant effective in improving the growth rate and survival rate (SR) in the closed hatchery recirculation system of catfish.
Uji Potensi Sari Buah Belimbing Wuluh (Averrhoa bilimbi L.) dalam Menghambat Pertumbuhan Bakteri Aeromonas salmonicida Smithia Secara In Vitro [Potential Test Cucumber Fruit Juice (Averrhoa bilimbi L.) In Inhibiting Growth Of Aeromonas salmonicida Smithia Bacteria By In Vitro] Prayogo Prayogo; Boedi Setya Rahardja; Rena Wilis Putri
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 2 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i2.11602

Abstract

Abstract Giving antibiotics continuously can cause A. salmonicida smithia become resistant and residues of antibiotics may pollute the water environment. One alternative to using traditional medicinal plants that are antibacterial. Advantages of using traditional medicinal plants are relatively more secure, easily available, inexpensive, does not cause resistance, and relatively harmless to the surrounding environment. The content of natural chemicals from cucumber fruit is known to have antibacterial effects namely, flavonoids and phenols. The purpose of this study to determine ability the cucumber fruit juice inhibit bacterial growth A. salmonicida smithia and to determine the best concentration to inhibit bacterial growth A. salmonicida smithia with ampicillin drugs for comparison by in vitro. This research was conducted in the Bacteriology Laboratory of Fish Quarantine Center of Sidoarjo and Human Genetic Laboratory the Institute of Tropical Disease Centre, Airlangga University, Surabaya. This research use done eksperimental method by in vitro, with a large measure of cucumber fruit juice inhibition zone around the existing paper disc and comparing it to a large zone of inhibition using ampicillin. Concentration dilution series cucumber fruit juice used during the research, began 2 gr/ 2 ml to the concentration of 0,0625 gr/ml. Result of research show at concentration 0,125 gr/ml still has ability to pursue bacterium A. smithia salmonicida, so that can be anticipated that effective cucumber fruit juice if used as by drug, because at small concentration can to pursue bacteria A. smithia salmonicida by in vitro.
Efektivitas Rasio Jumlah Pasangan Induk Ikan Hias Black Tetra (Gymnocorymbus ternetzi) Terhadap Hasil Pemijahan [The Effectiveness Of The Ratio Of The Number Of Pairs Of Parents Of Pair Of Parents Of Black Tetra (Gymnocorymbus ternetzi) Against The Result Of Spawning] Prayogo Prayogo
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 2 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i2.11610

Abstract

Abstract The purpose of this research is to know the effectiveness of the ratio of the number of pairs of parents against the result of the spawning of Black Tetra fish (Gymnocorymbus ternezi) about the number of egg produced, the rate conception (fertilization rate), the rate of hatching (hatching rate) and the survival rate of the fish (survival rate). The results shows that the ratio of number of pairs of black tetra fish parent has no effect but the greater the increase ratio of number of pairs of male parent black tetra fish then the number of eggs produced decrease.
Pemanfaatan Isi Rumen Sapi yang Difermentasikan dengan Bakteri Bacillus pumilus terhadap Kandungan Klorofil pada Kultur Dunaliella salina [Use of Content Cow Rumen Fermented With Bacillus pumilus Bacterial Chlorophyll Content On The Culture Dunaliella salina] Endang Dewi Masithah; Nur Choiriyah; Prayogo Prayogo
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 1 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i1.11630

Abstract

Abstract Dunaliella salina is one type of natural food that can be used in an attempt pembeniha rumen contents of cows is one of the waste obtained from the abattoir which is rich in nutrients. Synthesis of chlorophyll a and b require N and P. The purpose of this study was to determine the effect and the best dose utilization of rumen contents of cows and Bacillus pumilus in the culture of Dunaliella salina in chlorophyll content. The research design used was Completely Randomized Design (CRD) followed by Duncan multiple range test. Treatment research Dunaliella salina was cultured in 500 mL glass bottle with 5 treatments and 4 replications. Culture medium used was sea water that has salinity 30 ppt as much as 0.5 liters of rumen fluid is added 0.5 ml of solution that has been fermented with Bacillus pumilus in accordance with the treatment dose. The results showed that the use of fermenter bacteria Bacillus pumilus in cow rumen contents effect on chlorophyll content and dose fermenter rumen bacterium Bacillus pumilus in cattle that give the best chlorophyll content in Dunaliella salina culture fermentation is the dose intreatment C (7.5 % dose fermentation) reached 0.715653211aí¬molchl/cell on the first day.