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All Journal Jurnal Biosains Pascasarjana THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Humanism : Jurnal Pengabdian Masyarakat Empowerment: Jurnal Pengabdian Masyarakat Prosiding Seminar Nasional Pengabdian Kepada Masyarakat
Tri Ade Saputro
Program Studi Sarjana Terapan Teknologi Laboratorium Medik UM Surbaya
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Variasi Profil Protein Daging Sapi yang Dibungkus Daun Pepaya dengan Elektrophoresis SDS-Page Tri Ade Saputro
Jurnal Biosains Pascasarjana Vol. 22 No. 2 (2020): Jurnal Biosains Pascasarjana
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbp.v22i2.2020.46-49

Abstract

Abstrak Daging merupakan bahan pangan yang penting dalam memenuhi kebutuhan gizi.Protein pada daging lebih mudah dicerna dibanding protein yang berasal dari tumbuhan (nabati). Daun pepaya merupakan tanaman obat-obatan karena mengandung senyawa alkaloida dan enzim proteolitik, papain, khimopapain, dan lisosom,  yang  berguna pada proses pencernaan dan  mempermudah kerja usus Dalam pepaya terkandung enzim-enzim protease (pengurai protein) yaitu papain dan kimopapain. Kedua enzim ini mempunyai kemampuan menguraikan ikatan- ikatan dalam melekul protein sehingga protein terurai menjadi polipeptida dan dipeptide.Berdasarkan penelitian yang dilakukan di dapatkan hasil sebagai berikut pada tiap  sampelnya  sampel dengan rendaman  daun papaya selama 10  menit memiliki 11 pita protein, sampel rendaman daun papaya selama 20 menit memiliki 16 pita protein dan sampel rendaman daun papaya selama 30 menit memiliki 15 pita protein. Keywords: prifil protein, daging sapi, SDS page, elektroforesis AbstractBeef is an important food ingredient in meeting nutritional needs. Protein in beef is  easier  to  digest  than  protein  from  plants  (vegetables).  Papaya  leaf    is  a medicinal plant because it contains alkaloids and proteolytic enzymes, papain, khimopapain,  and  lyosomes,  which  are  useful  in  the  digestive  process  and facilitate the work of the intestines. Papaya contains protease enzymes (protein decomposers), papain and khimopapain. Both of these enzymes have the ability to decompose the bonds in the protein molecule so that the protein breaks down into polypeptides and dipeptides. Based on the research done in getting the following results in each sample, samples marinated with papaya leaf for 10 minutes have 11 protein bands, samples marinated during 20 minutes have 16 protein bands and samples marinated for 30 minutes have 15 protein bands. Kata Kunci: prifil protein, beef, SDS page, elektroforesis
Prevalensi Hasil Uji Saring Hbsag Pada Darah Donor Di Unit Tranfusi Darah (UTD) PMI Sampang Madura Rahma Widyastuti; Nur Vita Purwaningsih; Ellies Tunjung Sari Maulidiyanti; Tri Ade Saputro
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.12824

Abstract

The screening blood test is a stage that is carried out by each PMI to ensure the safety before blood transfusion. It is usually done on antibodies (Ab) such as anti-HCV, anti-HIV, TPHA or antigen (Ag) such as HBsAg. HBsAg is the outer coat protein of hepatitis B virus (HBV) and a sign that the individual has been infected with the hepatitis B virus. Hepatitis B virus can cause acute problems and even cirrhosis of the liver. One of the screening blood test methods which is used to detect hepatitis B is ELISA (Enzyme Linked Immuno Sorbent Assay) method. This research was conducted at UTD PMI Sampang by using the data collection in February 2020, and aimed to find out the overview of HBsAg examination results on the screening blood test using ELISA method based on age and sex. Based on the screening blood test, 9 people (3%) were reactive HBsAg and 345 people (97%) were non-reactive. In the age category, the highest number of reactive HBsAg donors was in the age group of 18-24 years old, that was 4 donors (1.1%), while the highest number of non-reactive HBsAg donors was in the age group of 25-44 years old, that was 184 people ( 51.9%). In the gender category, donors with the most reactive HBsAg results were male, that was 8 people (2.3%) and donors with the highest non-reactive HBsAg results was male, that was 283 people (79.9%). In order to minimize hepatitis B, it is expected that society should avoid activities which can cause hepatitis B infection. Keywords        : HBsAg, Blood Transfusion, Screening Blood Test 
EDUKASI PENGARUH KONSUMSI AIR SUMUR TERHADAP FUNGSI GINJAL DI JEMBER Rahma Widyastuti; Ellies Tunjung Sari M; Nur Vita Purwaningsih; Tri Ade Saputro; Nastiti Kartikorini
Humanism : Jurnal Pengabdian Masyarakat Vol 3, No 2 (2022): Agustus
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/hm.v3i2.14361

Abstract

 Air merupakan kebutuhan yang sangat penting bagi manusia, baik secara langsung maupun tidak langsung. Air merupakan kebutuhan mutlak untuk kelangsungan hidup. Dimana diatur dalam Peraturan Menteri Kesehatan Republik Indonesia No.416/Menkes/Per/XI/1990, tentang kualitas air bersih di daerah perbukitan atau di daerah pegunungan kapur menyebabkan kondisi sumber air bersih tercemar terutama di sumur gali di sekitarnya. penggunaan air sumur yang tercampur kapur secara berlebihan dan berulang dapat berdampak buruk bagi kesehatan tubuh, salah satunya penyakit ginjal. Kegiatan pengabdian berupa edukasi kreatif terkait pengaruh konsumsi air minum terhadap fungsi ginjal ini dilakuakan dengan metode ceramah. Kegiatan pengabdian masyarakat berupa edukasi kreatif agar masyarakat dapat lebih mudah memahami penyakit ginjal yang disebabkan oleh konsumsi air yang mengandung kapur baik terkait penyebab, bahaya, dampak, dan pencegahan secara baik. Melalui kegiatan ini terjalin kerjasama antara institusi perguruan tinggi dengan pemerintah daerah yaitu desa setempat dalam upaya mengurang faktor resiko penyakit ginjal. Kegiatan edukasi ini diharapkan dapat berkelanjutan sehingga masyarakat lebih memahami cara-cara pencegahan.
Penggunaan Berbagai Jenis Antikoagulan Terhadap Mutu Hasil Pemeriksaan Activated Partial Thromboplastine Time (APTT) Ellies Tunjung Sari Maulidiyanti; Fitrotin Azizah; Tri Ade Saputro; Firdausi Nuzula
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.14604

Abstract

Activated Partial Thromboplastine Time (APTT) is a type of hemostasis examination with citrate anticoagulant 3.2% ratio 1:9. Ethylene Diamine Tetraacetic Acid (EDTA) is one of the examination materials for hematology which is often used as an anticoagulant. Apart from EDTA, other types of anticoagulants are sodium citrate and potassium oxalate. The three anticoagulants work to bind calcium ions. The purpose of this examination is to determine the use of various anticoagulants on the quality of the results of the Activated Partial Thromboplastine Time (APCT) examination. The method used in this study is an experimental study with 3 sample groups, each consisting of 9 samples in one group. The SPSS results showed a probability value of 0.000 with the Anova test, the average results of the APTT examination were administration of sodium citrate anticoagulant 25.9 seconds, potassium oxalate 70.92 seconds, and EDTA 37.5 seconds. Based on the results of these studies, there are significant differences related to the use of various anticoagulants on the quality of the results of the Activated Partial Thromboplastine Time (APTT) examination.Keywords : Activated Partial Thromboplastine Time, Antikoagulans, 
Edukasi dan Pemeriksaan pada Ibu Hamil tentang Bahaya Infeksi Saluran Kemih pada Kehamilan Nur Vita Purwaningsih; Rahma Widyastuti; Firdausi Nuzula; Ellies Tunjung Sari; Ainutajriani Ainutajriani; Tri Ade Saputro
Empowerment: Jurnal Pengabdian Masyarakat Vol. 2 No. 2 (2023): MARET 2023
Publisher : Pusat Riset Manajemen dan Publikasi Ilmiah Serta Pengembangan Sumber Daya Manusia Sinergi Cendikia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55983/empjcs.v2i2.380

Abstract

Hormonal changes in pregnant women are a process of preparing for the birth of a baby, pregnant women are very sensitive to infections from various microorganisms. Urinary tract infection is the most common bacterial infection in pregnancy, increasing the risk of morbidity and mortality of mothers and newborns. A white blood cell count of more than 15,000/mm3 is an indication of infection in pregnant women. Caused by viral, bacterial and protozoal infections. Infection in pregnant women must be detected early, in order to prevent preterm labor. One of the tests that can be done is to count the number of erythrocytes and leukocytes. This study was conducted to determine the results of erythrocytes and leukocytes in pregnant women. Community service education for pregnant women about the dangers of urinary tract infections in pregnancy to educate and inform health. Community service activities in the form of creative education through the presentation of poster and presentations are carried out directly at the bancaran village hall. Community service activities in the form of creative education related to education for pregnant women about the dangers of urinary tract infections in pregnancy in the Bancaran sub-district area were carried out well, and received a positive enthusiastic response.
Edukasi Pemanfaatan Biji Asam Jawa (Tamarindus Indica L.) Sebagai Tindakan Preventif Obesitas Pada Remaja Rahma Widyastuti; Nur Vita Purwaningsih; Vella Rohmayani; Tri Ade Saputro; Waras Budiman; Hartono Kahar
PROSIDING SEMINAR NASIONAL PENGABDIAN KEPADA MASYARAKAT Vol. 3 No. 1 (2023): Prosiding Seminar Nasional Pengabdian Kepada Masyarakat : MEMAKSIMALKAN POTENSI
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/snpm.v3i1.1280

Abstract

Angka kejadian obesitas pada remaja terus meningkat dan berkontribusi terhadap risiko penyakit metabolik seperti diabetes tipe 2 dan dislipidemia, yang merupakan faktor risiko utama untuk penyakit kardiovaskular. Perubahan gaya hidup masyarakat yang mementingkan kemudahan dan kecepatan dalam mengonsumsi makanan cepat saji menyebabkan kurangnya asupan nutrisi yang dibutuhkan tubuh, dan cenderung mengonsumsi makanan dengan kandungan kolesterol tinggi. Sebagai alternatif pencegahan obesitas pemanfaatan biji asam jawa (Tamarindus indica L.) merupakan tanaman herbal biji asam jawa yang mengandung berbagai senyawa bioaktif seperti flavonoid, asam fenolat, vitamin C dan serat pangan. Metode yang digunakan pada kegiatan pengabdian masyarakat ini adalah presentasi dan diskusi tentang bahaya obesitas pada remaja. Untuk mengukur tingkat pemahaman peserta terkait bahaya obesitas, dilakukan pretest di awal dan dilakukan postest diakhir kegiatan. Kegiatan ini diikuti sebanyak 65 remaja, baik laki – laki maupun perempuan. Adapun rata – rata nilai pretest adalah sebesar 56,15 poin, sedangkan rata – rata nilai postest sebesar 81,23 poin. Hasil ini menunjukkan bahwa terjadi peningkatan pengetahuan bahaya obesitas pada remaja. Kegiatan edukasi pemanfaatan biji asam jawa (Tamarindus L Indica) sebagai penurunan penyakit kardiovaskular pada remaja dapat terlaksana dengan baik. Hal ini dibuktikan dengan peningkatan pengetahuan remaja yang dibuktikan dengan meningkatnya nilai rata – rata post-test. Kegiatan ini diharapkan dapat berlanjut dengan cara melakukan pencegahan obesitas dengan pemanfaatan ramuan tradisional.
Co-Authors Ainutajriani Ainutajriani Ellies Tunjung Sari Maulidiyanti Firdausi Nuzula Fitrotin Azizah, Fitrotin Hartono Kahar, Hartono Nastiti Kartikorini Nur Vita Purwaningsih, Nur Vita Rahma Widyastuti Rahma Widyastuti Vella Rohmayani Waras Budiman