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PENGARUH PENYULUHAN KESEHATAN TERHADAP PENGETAHUAN DAN SIKAP SANTRI DALAM MENCEGAH PENULARAN SKABIES DI PONDOK PESANTREN KECAMATAN KARANGGENENG KABUPATEN LAMONGAN TAHUN 2019 Afifah, Alvira Nadya Nur; Utomo, Budi; Pusarawati, Suhintam
(Jurnal Ilmiah Mahasiswa Kesehatan Masyarakat) Vol 6, No 1 (2021): JIMKesmas (Jurnal Ilmiah Mahasiswa Kesehatan Masyarakat)
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37887/jimkesmas.v6i1.16179

Angka kejadian penyakit skabies di Indonesia masih cukup tinggi terutama di area padat penghuni seperti PondokPesantren, namun pengetahuan mengenai skabies dan pencegahannya tidak diajarkan di Pondok Pesantren.Penelitian ini bertujuan untuk menganalisis pengaruh penyuluhan kesehatan terhadap pengetahuan dan sikapsantri dalam mencegah penularan skabies di Pondok Pesantren. Penelitian kuasi eksperimental dengan onegroup pretest-posttest design ini dilaksanakan pada bulan September 2019 di Pondok Pesantren KecamatanKaranggeneng Kabupaten Lamongan dengan melibatkan 96 responden yang diambil menggunakan teknikpurposive sampling. Pengumpulan data menggunakan kuesioner yang diberikan kepada responden sebelum dansesudah penyuluhan kesehatan. Hasil penelitian dapat diketahui bahwa kepadatan penghuni di PondokPesantren Kecamatan Karanggeneng Kabupaten Lamongan cukup tinggi dengan rata-rata luas ruangan setiapsantri adalah 0,5 meter. Analisis univariat pengetahuan dan sikap santri dalam mencegah penularan skabiessebelum diberikan penyuluhan kesehatan sebagian besar santri memiliki pengetahuan yang kurang (85,4%) dansikap yang cukup (78,1%), kemudian setelah diberikan penyuluhan kesehatan didapatkan peningkatan jumlahsantri dengan pengetahuan yang baik (44,8%) dan sikap yang baik (51%). Analisis bivariat pengaruh penyuluhankesehatan terhadap pengetahuan dan sikap santri dalam mencegah penularan skabies menggunakan ujiwilcoxon didapatkan nilai P sebesar 0,001. Dapat disimpulkan bahwa terdapat pengaruh penyuluhan kesehatanterhadap pengetahuan dan sikap santri dalam mencegah penularan skabies di Pondok Pesantren. Untuk itupenyuluhan kesehatan mengenai skabies dan pencegahannya sangat disarankan untuk dilaksanakan di PondokPesantren.Kata Kunci: Penyuluhan kesehatan, Pencegahan skabies, Pengetahuan, Sikap, Pondok PesantrenÂ

Breeding Preference and Bionomics of Anopheles spp. at the Malarial Endemic Area, Runut Village, East Nusa Tenggara Province, Indonesia Gery Morsales Munthe; David Nugraha; Gabriel Pedro Mudjianto; Etik Ainun Rohmah; Arnoldina Dolfina Dua Weni; Zukhaila Salma; Lynda Rossyanti; Fitriah; Suhintam Pusarawati; Budi Utomo; ukmawati Basuki; Haruki Uemura
Biomolecular and Health Science Journal Vol. 5 No. 1 (2022): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bhsj.v5i1.35278

Introduction: Anopheles mosquito is transmitting malaria, one of the health problems in Indonesia. Understanding Anopheles mosquito behaviour and its breeding preference is one of the crucial keys to prevent malaria transmission. This study aimed to identify the breeding place distribution and bionomics of Anopheles spp. in Runut village, Sikka district, East Nusa Tenggara. Methods: A descriptive observational study was conducted in Runut village, Waigete sub- district, in April 2018. Anopheles spp. larvae were collected in 7 suspected breeding places consisting of 2 rice fields, 3 fish ponds, and 2 puddles. Mosquitos behaviour was observed using bed-net traps located inside and outside the house from 7.15 PM to 1.15 AM after obtaining informed consent. Mosquito collection using bed-net trap were performed for 40 minutes then followed by resting mosquito collection for 10 minutes. Results: Anopheles spp. larvae were found in most of the suspected aquatic habitats, presenting different densities and together with larvae of the other mosquito species. Relatively high number of Anopheles spp. larvae was obtained from a puddle. Only one female mosquito of Anopheles spp. resting on the wall inside house was found around 00.55 – 01.05 AM and resulted in low mosquito density determination. Conclusion: Anopheles spp. larvae were harbouring in most of the aquatic habitats and one puddle contained moderately abundant larvae of Anopheles in Runut village, Sikka district, East Nusa Tenggara, Indonesia. Even only one Anopheles spp. mosquito was detected inside the house, residents in Runut village should regular use insecticide-treated bed nets and continuous observation of mosquito breeding places especially puddles to prevent malaria and other mosquito-borne diseases.

PENYULUHAN DAN PELATIHAN DENGUE PADA IBU SERTA DETEKSI DINI INFEKSI DENGUE PADA PASIEN DENGAN SUSPEK INFEKSI DENGUE DI TULUNGAGUNG, JAWA TIMUR Juniastuti Juniastuti; Lynda Rossyanti; Manik R Wahyunitisari; Achmad Ardianto; Rebekah J Setiabudi; Suhintam Pusarawati; Dewi Setyowati; Putri S Wulandari
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 4 No. 1 (2020): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v4i1.2020.230-236

Tulungagung Regency in East Java, is a Dengue endemic area, but the movement of 1 house 1 jumantik / larva monitor (Government program for controlling Dengue vectors), which involves every family in the inspection, monitoring and eradication of mosquito larvae, has not yet been implemented. The objectives of this community service activity are 1) increasing maternal knowledge about Dengue, 2) increasing maternal efforts in eradicating Aedes mosquito larvae (Dengue vectors) and 3) improving mother's skills in monitoring mosquito larvae in Karangrejo District; and 4) determine the proportion of early dengue infection at the Karangrejo Health Center. The method used is counseling, training and laboratory examinations (rapid test with the NS1 Dengue kit). This community service obtained the conclusion of counseling can increase the knowledge of dengue in mothers, counseling can increase maternal efforts in eradicating mosquito larvae, training to improve the skills of mothers in monitoring mosquito larvae, there are 10.8% of patients with symptoms of dengue infection who show positive NS1.abstrakKabupaten Tulungagung di Jawa Timur, merupakan daerah endemis Dengue, namun gerakan 1 rumah 1 jumantik/juru pemantau jentik (program Pemerintah untuk pengendalian vektor Dengue) yaitu yang melibatkan setiap keluarga dalam pemeriksaan, pemantauan, dan pemberantasan jentik nyamuk, masih belum banyak diterapkan. Tujuan kegiatan pengabdian masyarakat ini adalah 1) meningkatkan pengetahuan ibu tentang Dengue, 2) meningkatkan upaya ibu dalam memberantas tempat perindukan jentik nyamuk Aedes (vektor Dengue) dan 3) meningkatkan ketrampilan ibu dalam memantau jentik nyamuk di Kecamatan Karangrejo; serta 4) menentukan proporsi kejadian infeksi Dengue secara dini di Puskesmas Karangrejo. Metode yang digunakan adalah penyuluhan, pelatihan dan pemeriksaan laboratorium (uji cepat dengan kit NS1 Dengue). Pengmas ini memperoleh simpulan penyuluhan dapat meningkatkan pengetahuan dengue pada ibu, penyuluhan dapat meningkatkan upaya ibu dalam memberantas tempat perindukan jentik nyamuk, pelatihan meningkatkan ketrampilan ibu dalam memantau jentik nyamuk, ada 10,8% pasien dengan gejala infeksi Dengue yang menunjukkan NS1 positif.

Prevalence of Expanded Dengue Syndrome in Patients with Dengue Virus Infection at the Dr. Soetomo Hospital Surabaya in 2017 – 2018 Avina Fimas Adissadah; Aryati Aryati; Suhintam Pusarawati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 3 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i3.1589

Dengue Fever (DF) or Dengue Hemorrhagic Fever (DHF) is one of the infectious diseases that attracts much global attention, especially Indonesia because it impacts the mortality rate of the people in the world if adequate treatment is not given. Expanded Dengue Syndrome (EDS) is a clinical manifestation involving several organs such as lungs, liver, kidney, heart, and brain-related to dengue infections, with or without plasma leakage. This study aimed to determine the prevalence of expanded dengue syndrome in patients with dengue virus infection at the Dr. Soetomo Hospital in 2017–2018. Based on its purpose, the sampling technique used in this study was the total sampling of patients with DVI who admitted to Dr. Soetomo Hospital Surabaya. Every pediatric and adult patient who met the criteria were included in this study until a certain period in 2017–2018. After the data collection, only thirty samples of EDS from the 196 sample data were collected. Thirty patients with expanded dengue syndrome had a manifestation of different organs. Such as: neurological, cardiac, respiratory system, gastro-hepatic, and kidneys. The laboratory test results showed that most platelet counts of 51,000 – 100,000 were found in seventeen patients, while the range of hemoglobin and hematocrit was normal. From a total of thirty samples of dengue virus infection patients with EDS, there were only eighteen patients who did a serological test. The most found serological results were (+/-) anti-dengue IgM and (+) anti-dengue IgG (40%).

Prevalence of Escherichia Coli, Salmonella Sp, Staphylococcus Aureus Bacteria in Chicken Meat of Traditional Market Surabaya City Ulfa Dwi Karisma; Nurul Wiqoyah; Suhintam Pusarawati
Jurnal Ilmu dan Teknologi Kesehatan Vol 8 No 2 (2021): Maret 2021
Publisher : Poltekkes Kemenkes Jakarta III

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32668/jitek.v8i2.510

Foodborne disease is a disease caused by contaminated food. Chicken meat is very susceptible to contamination by bacteria. Escherichia coli, Salmonella sp, and Staphylococcus aureus are types of bacteria found in chicken meat. The purpose of this study was to see how many Escherichia coli, Salmonella sp, and Staphylococcus aureus colonies in raw chicken meat in traditional markets in Surabaya City. The research sample used was 30 samples of chicken meat taken in 5 traditional markets in Surabaya. Six chicken meat sellers were taken with 1 sample and put in the sterile tube from each market. The test was carried out using the Total Plate Count (TPC) method. The TPC test results showed that all samples were contaminated with E. coli, with the most colony being 2.2X10-7 CFU/g while the minor colony was 5X10-4 CFU/g. There are two samples contaminated with Salmonella sp, in the G market and the W market, with code D6 4.7X10-6 CFU/g and code E5 1X10-6 CFU/g. Meanwhile, S. aureus was only found in the W market with sample codes E1 & E6, and each sample had a colony of 2.7X 10-7 CFU/g and 2X10-5 CFU/g.

Profil Indeks Larva pada Delapan Wilayah Puskesmas di Surabaya LYNDA ROSSYANTI; DWI SUSANTI; IVAN RAHMATULLAH; DWI PENI KARTIKASARI; SUKMAWATI BASUKI; HENY ARWATI; INDAH TANTULAR; SUHINTAM PUSARAWATI; HERU PRASETYO; SRI HIDAJATI; SUBAGYO YOTOPRANOTO; SRI WIJAYANTI SULISTYAWATI
HANG TUAH MEDICAL JOURNAL Vol 17 No 2 (2020): Hang Tuah Medical Journal
Publisher : Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar

ABSTRACT Background : Dengue virus infection is still a health problem in Surabaya. On 2015 Insidence Rate (IR) for Surabaya is 22.50 and the Case Fatality Rate (CFR) is 2%. This figure has decreased compared to 2014, but has not met the target achievement indicator for the P2DBD target CFR <1%. Method : The study was conducted in the rainy season, May 2015 in several Puskesmas areas in Surabaya. Samples were randomly selected as many as 378 houses spread in eight health centers (Pegirian, Krembangan Selatan, Hamlet Wall, Tambak Rejo, Putat Jaya, Simomulyo, Sawahan and Tanahkali Kedinding). The larvae survey was conducted using the single larva method. Larvae were taken from breeding sites and stored in specimen bottles for species identification at the Parasitology Laboratory Faculty of Medicine Universitas Airlangga. Result : The highest House Index (HI) was obtained (60.46%) or the 39.54% larvae free rate in Simomulyo and the lowest HI (33.33%) or 66.67% ABJ in Pegirian Puskesmas. Larvae species identification showed 100% was Aedes aegypti. The high HI value at the Simomulyo Health Center is not in line with the number of cases of dengue virus infection in 2015 which was 4 cases. Whereas in Pegirian Health Center with the lowest HI value found 11 cases. Discussion : These results could be due to the possibility because the study did not calculate larval density and high population mobility in the area.

Prevalence of Expanded Dengue Syndrome in Patients with Dengue Virus Infection at the Dr. Soetomo Hospital Surabaya in 2017 – 2018 Avina Fimas Adissadah; Aryati Aryati; Suhintam Pusarawati
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 26 No. 3 (2020)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i3.1589

Dengue Fever (DF) or Dengue Hemorrhagic Fever (DHF) is one of the infectious diseases that attracts much global attention, especially Indonesia because it impacts the mortality rate of the people in the world if adequate treatment is not given. Expanded Dengue Syndrome (EDS) is a clinical manifestation involving several organs such as lungs, liver, kidney, heart, and brain-related to dengue infections, with or without plasma leakage. This study aimed to determine the prevalence of expanded dengue syndrome in patients with dengue virus infection at the Dr. Soetomo Hospital in 2017–2018. Based on its purpose, the sampling technique used in this study was the total sampling of patients with DVI who admitted to Dr. Soetomo Hospital Surabaya. Every pediatric and adult patient who met the criteria were included in this study until a certain period in 2017–2018. After the data collection, only thirty samples of EDS from the 196 sample data were collected. Thirty patients with expanded dengue syndrome had a manifestation of different organs. Such as: neurological, cardiac, respiratory system, gastro-hepatic, and kidneys. The laboratory test results showed that most platelet counts of 51,000 – 100,000 were found in seventeen patients, while the range of hemoglobin and hematocrit was normal. From a total of thirty samples of dengue virus infection patients with EDS, there were only eighteen patients who did a serological test. The most found serological results were (+/-) anti-dengue IgM and (+) anti-dengue IgG (40%).

Distribution of Voltage Gated Sodium Channel (VGSC) Gene Mutational Variation And Acetylcholinesterase-1 (ACE-1) as A Marker For Insecticide Resistance In Culex spp. Mosquitoes In Surabaya Muhammad Akbar Panjinegara; Sukmawati Basuki; Dominicus Husada; Suhintam Pusarawati
Jurnal Indonesia Sosial Sains Vol. 5 No. 02 (2024): Jurnal Indonesia Sosial Sains
Publisher : CV. Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/jiss.v5i02.963

Culex spp. is a vector for Japanese Encephalitis (JE) and filariasis in Indonesia. JE is caused by Japanese Encephalitis Virus (JEV). JEV attacks the central nervous system and causes inflammation of the brain, lifelong disability, and even death. Filariasis is a chronic infectious disease that can cause permanent disability. Transmission of JE and filariasis can increase due to the density of Culex spp mosquitoes. The purpose of this study was to identify the distribution of mutation variations in the Voltage Gated Sodium Channel (VGSC) and acetylcholinesterase-1 (ace-1) genes in Culex spp. mosquitoes in Surabaya. Mutation variations of the VGSC Culex spp. gene show that there are three genotypes, namely wildtype (LL), heterozygous (LS, FS), and homozygous (FF, SS). Heterozygous (FS) and homozygous (FF, FS) genotypes caused by point mutations in codon 1014 of the VGSC gene are found in the Culex spp. mosquito population in Surabaya. Heterozygous (LF) genotype was not detected in the Culex spp. mosquito population in Surabaya. Another study in Nigeria showed that homozygous/FF genotypes (45.1%) were more prevalent than heterozygotes/LF (36.3%) and wildtypes/LL (18.6%).

The Longevity of Aedes aegypti Larvae in Several Water Sources in Surabaya Antonio Ayrton Widiastara; Gabriel Pedro Mudjianto; Etik Ainun Rohmah; Hengki Anggara Putra; Martha Indah Widia Ningtyas; Sri Wijayanti Sulistyawati; Suhintam Pusarawati; Fitriah; Kasiyama Desi Indriyani; Alpha Fardah Athiyyah; Basuki, Sukmawati
Indonesian Journal of Tropical and Infectious Disease Vol. 10 No. 1 (2022)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v10i1.32209

Aedes aegypti transmits the dengue virus that causes Dengue Viring the high number of DVI cases is the existing breeding places of Ae. aegypti. The water sources used by the community and the surrounding environment are essential media for living Ae. aegypti larvae. This recent study aimed to detect the longevity of Ae. aegypti larvae in diï¬€ erent water sources in Surabaya and the killing eï¬€ect of temephos. An analytical observational and experimental study was conducted in August-September 2021. Twenty-instar III Ae. aegypti larvae were put in each 100 ml beaker glass containing diï¬€ erent water sources, such as rain, well, mineral, new and used bath water, and antiseptic soapy water. Fungi in water sources were examined. Two groups were set with and without temephos, the ï¬nal temephos concentration was of 0.00001 ppm. Live Ae. aegypti larvae, pupae, mosquitoes were observed every 24 hours for seven days without feeding. Living larvae were still found on Day 7 in all water sources with and without temephos. There were more larvae live in soapy water without temephos, particularly on Day 2 to Day 6, compared to other water sources either without or with temephos. In contrast, many larvae died in mineral water with temephos. Some larvae turned into pupae, started on Day 1. Pupae and mosquitoes were mostly found in rain water with temephos. Ae. aegypti larvae survived better in soapy water either with or without temephos. Temephos seemed to be eï¬€ective to kill Ae. aegypti larvae in mineral water, and might induce larvae in turning to pupae and mosquitoes quickly at low concentration.

The Polymorphism of Voltage-Gated Sodium Channel Gene in Culex Mosquitoes in Banyuwangi District, Indonesia Yasmin Azzahra Arifin; Hanifah Dzakiyah; Catherine Canina Charunita; Sakura Alya Ashari; Winna Putri Veliarta; Muhammad Akbar Panjinegara; Francesca Giovanetti; Carlo Montagna; Wojciech Jankowski; Muhammad Fathoni; Binarri Augustya; Lynda Rossyanti; Suhintam Pusarawati; Sukmawati Basuki
Andalasian International Journal of Entomology Vol. 2 No. 2 (2024)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/aijent.2.2.106-113.2024

Culex mosquito is able to act as transmission vector of filariasis and Japanese encephalitis. Both diseases occur in Indonesia and filariasis exists in Banyuwangi district. The Voltage-Gated Sodium Channel (VGSC) gene mutations are related to pyrethroid insecticide resistance, especially L1014F/S mutations have not yet been fully studied in Indonesia. This study aimed to investigate the polymorphism of VGSC gene mutation in Culex mosquitoes in Banyuwangi District of East Java Province, Indonesia. Culex mosquitoes were collected using mosquito trap in a two-day collection from Banyuwangi Sub-district and two sub-villages in Cluring Sub-district of Banyuwangi District in August 2023. Culex mosquitoes were identified by morphological examination and Culex DNA was extracted by Chelex100 from each Culex mosquito. The polymorphism of VGSC gene mutations of L1014F/S were determined by Allele Specific-Polymerase Chain Reaction (AS-PCR). A total of 62 Culex mosquitoes were analyzed. The wild type Culex mosquitoes were found predominant (77%, 48/62). Two types of homozygous mutations were identified as 1014F and 1014 S (16%, 10/62 and 2%, 1/62, respectively). The heterozygous mutation was only 1014F/S type with frequency of 5% (3/62). The predominance of wild type Culex mosquitoes indicated that Culex population in the study areas is still susceptible to pyrethroid insecticide. However, the mutant types of Culex are already occurred, so insecticide resistance among Culex mosquitoes is required to be regular monitoring.

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