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All Journal Indonesian Journal of Tropical and Infectious Disease JIMKesmas (Jurnal Ilmiah Mahasiswa Kesehatan Masyarakat) Jurnal Ilmu dan Teknologi Kesehatan Biomolecular and Health Science Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Jurnal Layanan Masyarakat (Journal of Public Service) Hang Tuah Medical Journal Jurnal Indonesia Sosial Sains Andalasian International Journal of Entomology
Suhintam Pusarawati
Department Of Parasitology, Faculty Of Medicine, Airlangga University, Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Energy Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Distribution of Voltage Gated Sodium Channel (VGSC) Gene Mutational Variation And Acetylcholinesterase-1 (ACE-1) as A Marker For Insecticide Resistance In Culex spp. Mosquitoes In Surabaya Akbar Panjinegara, Muhammad; Basuki, Sukmawati; Husada, Dominicus; Pusarawati, Suhintam
Jurnal Indonesia Sosial Sains Vol. 5 No. 02 (2024): Jurnal Indonesia Sosial Sains
Publisher : CV. Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/jiss.v5i02.963

Abstract

Culex spp. is a vector for Japanese Encephalitis (JE) and filariasis in Indonesia. JE is caused by Japanese Encephalitis Virus (JEV). JEV attacks the central nervous system and causes inflammation of the brain, lifelong disability, and even death. Filariasis is a chronic infectious disease that can cause permanent disability. Transmission of JE and filariasis can increase due to the density of Culex spp mosquitoes. The purpose of this study was to identify the distribution of mutation variations in the Voltage Gated Sodium Channel (VGSC) and acetylcholinesterase-1 (ace-1) genes in Culex spp. mosquitoes in Surabaya. Mutation variations of the VGSC Culex spp. gene show that there are three genotypes, namely wildtype (LL), heterozygous (LS, FS), and homozygous (FF, SS). Heterozygous (FS) and homozygous (FF, FS) genotypes caused by point mutations in codon 1014 of the VGSC gene are found in the Culex spp. mosquito population in Surabaya. Heterozygous (LF) genotype was not detected in the Culex spp. mosquito population in Surabaya. Another study in Nigeria showed that homozygous/FF genotypes (45.1%) were more prevalent than heterozygotes/LF (36.3%) and wildtypes/LL (18.6%).
The Polymorphism of Voltage-Gated Sodium Channel Gene in Culex Mosquitoes in Banyuwangi District, Indonesia Yasmin Azzahra Arifin; Hanifah Dzakiyah; Catherine Canina Charunita; Sakura Alya Ashari; Winna Putri Veliarta; Muhammad Akbar Panjinegara; Francesca Giovanetti; Carlo Montagna; Wojciech Jankowski; Muhammad Fathoni; Binarri Augustya; Lynda Rossyanti; Suhintam Pusarawati; Basuki, Sukmawati
Andalasian International Journal of Entomology Vol. 2 No. 2 (2024)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/aijent.2.2.106-113.2024

Abstract

Culex mosquito is able to act as transmission vector of filariasis and Japanese encephalitis. Both diseases occur in Indonesia and filariasis exists in Banyuwangi district. The Voltage-Gated Sodium Channel (VGSC) gene mutations are related to pyrethroid insecticide resistance, especially L1014F/S mutations have not yet been fully studied in Indonesia. This study aimed to investigate the polymorphism of VGSC gene mutation in Culex mosquitoes in Banyuwangi District of East Java Province, Indonesia. Culex mosquitoes were collected using mosquito trap in a two-day collection from Banyuwangi Sub-district and two sub-villages in Cluring Sub-district of Banyuwangi District in August 2023. Culex mosquitoes were identified by morphological examination and Culex DNA was extracted by Chelex100 from each Culex mosquito. The polymorphism of VGSC gene mutations of L1014F/S were determined by Allele Specific-Polymerase Chain Reaction (AS-PCR). A total of 62 Culex mosquitoes were analyzed. The wild type Culex mosquitoes were found predominant (77%, 48/62). Two types of homozygous mutations were identified as 1014F and 1014 S (16%, 10/62 and 2%, 1/62, respectively). The heterozygous mutation was only 1014F/S type with frequency of 5% (3/62). The predominance of wild type Culex mosquitoes indicated that Culex population in the study areas is still susceptible to pyrethroid insecticide. However, the mutant types of Culex are already occurred, so insecticide resistance among Culex mosquitoes is required to be regular monitoring.
Co-Authors Achmad Ardianto Afifah, Alvira Nadya Nur Akbar Panjinegara, Muhammad Alpha Fardah Athiyyah Antonio Ayrton Widiastara Arnoldina Dolfina Dua Weni Aryati Aryati Avina Fimas Adissadah Binarri Augustya BUDI UTOMO Budi Utomo Carlo Montagna Catherine Canina Charunita David Nugraha Dewi Setyowati Dominicus Husada Dwi Susanti Fitriah Francesca Giovanetti Gabriel Pedro Mudjianto Gery Morsales Munthe Hanifah Dzakiyah Haruki Uemura Hengki Anggara Putra Heny Arwati INDAH TANTULAR Juniastuti Juniastuti KARTIKASARI, DWI PENI Kasiyama Desi Indriyani Manik R Wahyunitisari Martha Indah Widia Ningtyas Muhammad Akbar Panjinegara Nurul Wiqoyah, Nurul Putri S Wulandari R. Heru Prasetyo Rahmatullah, Ivan Rebekah Setiabudi, Rebekah Rohmah, Etik Ainun Rossyanti, Lynda Sakura Alya Ashari SRI HIDAJATI Sriwijayanti Sulistyawati SUBAGYO YOTOPRANOTO Sukmawati Basuki ukmawati Basuki Ulfa Dwi Karisma Winna Putri Veliarta Wojciech Jankowski Yasmin Azzahra Arifin Zukhaila Salma